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Title: Method for producing 3-hydroxypropionic acid and other products


This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes 3-hydroxypropionic acid.

; ;
Issue Date:
Research Org.:
Cargill, Incorporated, Wayzata, MN (United States)
Sponsoring Org.:
OSTI Identifier:
Patent Number(s):
Application Number:
Cargill, Incorporated (Wayzata, MN)
Patent Classifications (CPCs):
DOE Contract Number:  
Resource Type:
Resource Relation:
Patent File Date: 2016 May 27
Country of Publication:
United States

Citation Formats

Lynch, Michael D., Gill, Ryan T., and Lipscomb, Tanya E. W. Method for producing 3-hydroxypropionic acid and other products. United States: N. p., 2018. Web.
Lynch, Michael D., Gill, Ryan T., & Lipscomb, Tanya E. W. Method for producing 3-hydroxypropionic acid and other products. United States.
Lynch, Michael D., Gill, Ryan T., and Lipscomb, Tanya E. W. Tue . "Method for producing 3-hydroxypropionic acid and other products". United States.
title = {Method for producing 3-hydroxypropionic acid and other products},
author = {Lynch, Michael D. and Gill, Ryan T. and Lipscomb, Tanya E. W.},
abstractNote = {This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes 3-hydroxypropionic acid.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {10}

Works referenced in this record:

The overexpression and complete amino acid sequence of Escherichia coli 3-dehydroquinase
journal, September 1986

Construction of an Escherichia coli K-12 Mutant for Homoethanologenic Fermentation of Glucose or Xylose without Foreign Genes
journal, January 2007

Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine
journal, September 1999

Engineering Central Metabolic Pathways for High-Level Flavonoid Production in Escherichia coli
journal, April 2007

Enzymatic Conversion of β-Hydroxypropionate to Malonic Semialdehyde
journal, July 1959

Developments in Directed Evolution for Improving Enzyme Functions
journal, August 2007

Metabolic engineering of aromatic group amino acid pathway in Bacillus subtilis for L-phenylalanine production
journal, November 2004

SCALEs: multiscale analysis of library enrichment
journal, November 2006

Induction of specific base-pair substitutions in E. coli trpA mutants by chloroenthylene oxide, a carcinogenic vinyl chloride metabolite
journal, November 1985

Challenges in Engineering Microbes for Biofuels Production
journal, February 2007

Sequence and overexpression of the menD gene from Escherichia coli.
journal, August 1989

Lactate-Utilizing Bacteria, Isolated from Human Feces, That Produce Butyrate as a Major Fermentation Product
journal, October 2004

Inhibitory activity spectrum of reuterin produced by Lactobacillus reuteri against intestinal bacteria
journal, January 2007

Role of bicarbonate/CO2 in the inhibition of Escherichia coli growth by cyanate.
journal, June 1995

A mild and efficient method for esterification and transesterification catalyzed by iodine
journal, January 2002

Variant tricarboxylic acid cycle in Mycobacterium tuberculosis: Identification of α-ketoglutarate decarboxylase
journal, July 2005

Interdomain Communication between the Thiolation and Thioesterase Domains of EntF Explored by Combinatorial Mutagenesis and Selection
journal, August 2006

DNA shuffling of a family of genes from diverse species accelerates directed evolution
journal, January 1998

Simultaneous synthesis of enantiomerically pure (S)-amino acids and (R)-amines using coupled transaminase reactions
journal, January 2003

Expression of proteins encoded by the Escherichia coli cyn operon: carbon dioxide-enhanced degradation of carbonic anhydrase.
journal, September 1994

Beta-alanine synthesis in Escherichia coli.
journal, January 1980

Engineering primary metabolic pathways of industrial micro-organisms
journal, March 2007

Effect of poxB gene knockout on metabolism in Escherichia coli based on growth characteristics and enzyme activities
journal, September 2006

The qmeA (ts) mutation of Escherichia coli is localized in the fabI gene, which encodes enoyl-ACP reductase
journal, January 1996

Effect of process parameters on 3-hydroxypropionic acid production from glycerol using a recombinant Escherichia coli
journal, April 2009

A genomics approach to improve the analysis and design of strain selections
journal, May 2008

Cloning and sequencing of Escherichia coli ubiC and purification of chorismate lyase.
journal, August 1992

CHEMISTRY: Sugary Recipe Boosts Grow-Your-Own Plastics
journal, June 2006

Structure of Cellulases and Their Applications
journal, April 1997

Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli
journal, December 2002

Aminotransferases: demonstration of homology and division into evolutionary subgroups
journal, June 1993

Improving cellular malonyl-CoA level in Escherichia coli via metabolic engineering
journal, May 2009

Engineering of a functional human NADH-dependent cytochrome P450 system
journal, January 2001

Regulation of fatty acid biosynthesis in Escherichia coli.
journal, January 1993

A new perspective on thiamine catalysis
journal, August 2004

Effect of Overexpression of Actinobacillus succinogenes Phosphoenolpyruvate Carboxykinase on Succinate Production in Escherichia coli
journal, February 2004

The catabolite repressor/activator (Cra) protein of enteric bacteria.
journal, June 1996

The Rut Pathway for Pyrimidine Degradation: Novel Chemistry and Toxicity Problems
journal, April 2010

Fe2(SO4)3·4H2O/concentrated H2SO4: an efficient catalyst for esterification
journal, March 2004

Polyphosphate Kinase Protects Salmonella enterica from Weak Organic Acid Stress
journal, April 2005

Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different
journal, April 2001

Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids
journal, November 1998

Silent Polymorphisms Speak: How They Affect Pharmacogenomics and the Treatment of Cancer
journal, October 2007

A new preparation of trifluoromethanesulfinate salts
journal, July 2007

Extractive recovery of products from fermentation broths
journal, April 1999

Feasibility of acrylic acid production by fermentation
journal, February 2005

The IdhA Gene Encoding the Fermentative Lactate Dehydrogenase of Escherichia Coli
journal, January 1997

Nucleotide sequence of the Escherichia coli cad operon: a system for neutralization of low extracellular pH.
journal, April 1992

Sequencing and overexpression of the Escherichia coli aroE gene encoding shikimate dehydrogenase
journal, January 1988

Structure−Cytoprotective Activity Relationship of Simple Molecules Containing an α,β-Unsaturated Carbonyl System
journal, June 1997

Characterization of two temperature-inducible promoters newly isolated from B. subtilis
journal, July 2007

Control Mechanisms in the Synthesis of Saturated Fatty Acids
journal, June 1977

Thermal regulation of membrane lipid fluidity in bacteria
journal, February 1983

Genetic and biochemical analyses of pantothenate biosynthesis in Escherichia coli and Salmonella typhimurium.
journal, January 1982

Polypeptide Release Factors in Prokaryotes and Eukaryotes
journal, January 2002

Cloning, crystallization and preliminary characterization of a β-carbonic anhydrase from Escherichia coli
journal, September 2000

Engineering a mevalonate pathway in Escherichia coli for production of terpenoids
journal, June 2003

Network rigidity and metabolic engineering in metabolite overproduction
journal, June 1991

On the Role of Helix 0 of the Tryptophan Synthetase α Chain of Escherichia coli
journal, June 1996

The commercial production of chemicals using pathway engineering
journal, December 2000

Selective production of L-aspartic acid and L-phenylalanine by coupling reactions of aspartase and aminotransferase in Escherichia coli
journal, July 2000

Exploring the diversity of complex metabolic networks
journal, December 2004

Prediction of protein function from protein sequence and structure
journal, January 1999

Molecular breeding of carotenoid biosynthetic pathways
journal, July 2000

Genes restoring redox balance in fermentation-deficient E. coli NZN111
journal, November 2009

Improving lycopene production in Escherichia coli by engineering metabolic control
journal, May 2000

Directed evolution of RuBisCO hypermorphs through genetic selection in engineered E.coli
journal, January 2006

Reactions of β-lactones with potassium alkoxides and their complexes with 18-crown-6 in aprotic solvents
journal, July 1993

De novo biosynthetic pathways: rational design of microbial chemical factories
journal, October 2008

?-hydroxypropionic acid production by Byssochlamys sp. grown on acrylic acid
journal, November 1993

3-phenylpropionate catabolism and the Escherichia coli oxidative stress response
journal, April 2005

FolM, A New Chromosomally Encoded Dihydrofolate Reductase in Escherichia coli
journal, November 2003

Cloning and Overexpression in Soluble Form of Functional Shikimate Kinase and 5-Enolpyruvylshikimate 3-Phosphate Synthase Enzymes from Mycobacterium tuberculosis
journal, August 2001

Fermentation of Glucose to Lactic Acid Coupled with Reactive Extraction:  A Review
journal, September 2004

Biosynthetic pathways for 3-hydroxypropionic acid production
journal, February 2009

Human Catechol-O-Methyltransferase Haplotypes Modulate Protein Expression by Altering mRNA Secondary Structure
journal, December 2006

A T42M Substitution in Bacterial 5-Enolpyruvylshikimate-3-phosphate Synthase (EPSPS) Generates Enzymes with Increased Resistance to Glyphosate
journal, January 2003

A novel methodology employing Corynebacterium glutamicum genome information to generate a new L -lysine-producing mutant
journal, January 2002

Identification of PhoB binding sites of the yibD and ytfK promoter regions in Escherichia coli
journal, April 2011

Overproduction of phage Lambda repressor under control of the lac promotor of Escherichia coli
journal, January 1976

Naturally Occurring Adenines within mRNA Coding Sequences Affect Ribosome Binding and Expression in Escherichia coli
journal, November 2006

Inhibiting Bacterial Fatty Acid Synthesis
journal, April 2006

The biological degradation of cellulose
journal, January 1994

A core catalytic domain of the TyrA protein family: arogenate dehydrogenase from Synechocystis
journal, August 2004

Genetic Evidence for an Interaction of the UbiG O-Methyltransferase with UbiX in Escherichia coli Coenzyme Q Biosynthesis
journal, August 2006

Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design
journal, August 2005

Purification and characterization of 2-oxoglutarate decarboxylase of Leuconostoc oenos
journal, August 1990

Genome shuffling of Lactobacillus for improved acid tolerance
journal, July 2002

Toward a science of metabolic engineering
journal, June 1991

Dihydrolipoamide Dehydrogenase Mutation Alters the NADH Sensitivity of Pyruvate Dehydrogenase Complex of Escherichia coli K-12
journal, March 2008

Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis
journal, May 2003

Biorefineries:  Current Status, Challenges, and Future Direction
journal, July 2006

Light-directed, spatially addressable parallel chemical synthesis
journal, February 1991

Characterization of Mutations Contributing to Sulfathiazole Resistance in Escherichia coli
journal, January 1998

Unprecedented acetoacetyl-coenzyme A synthesizing enzyme of the thiolase superfamily involved in the mevalonate pathway
journal, June 2010

Rapid dissection of a complex phenotype through genomic-scale mapping of fitness altering genes
journal, May 2010

A process for microbial hydrocarbon synthesis: Overproduction of fatty acids in Escherichia coli and catalytic conversion to alkanes
journal, June 2010

A New Enzymatic Method of Acrylamide Production
journal, May 1982

Inverse metabolic engineering: A strategy for directed genetic engineering of useful phenotypes
journal, July 2002

Switch of coenzyme specificity of p -hydroxybenzoate hydroxylase 1 1Edited by A. R. Fersht
journal, September 1999

Characterization of the major promoter for the plasmid-encoded sucrose genes scrY, scrA, and scrB.
journal, December 1991

Synthesis of labeled acrylamide andN-methylolacrylamide (NMA):15N-acrylamide,13C-NMA,15N-NMA, and13C,15N-NMA
journal, January 2005

Application of Functional Genomics to Pathway Optimization for Increased Isoprenoid Production
journal, March 2008

Identification of a 21 amino acid peptide conferring 3-hydroxypropionic acid stress-tolerance to Escherichia coli
journal, January 2012

The tyrosine repressor negatively regulates aroH expression in Escherichia coli.
journal, June 1991

Method for purifying lactic acid
patent, December 2002

Identification and cloning of the Mycobacterium avium folA gene, required for dihydrofolate reductase activity
journal, November 1997

A "Silent" Polymorphism in the MDR1 Gene Changes Substrate Specificity
journal, January 2007

A Single Mutation Converts a Novel Phosphotyrosine Binding Domain into a Dual-specificity Phosphatase
journal, November 1995

Purification and Characterization of Methylmalonate-semialdehyde Dehydrogenase from Rat Liver
journal, September 1989

A Physiology Study of Escherichia coli Overexpressing Phosphoenolpyruvate Carboxykinase
journal, April 2008

Sequences of the envM gene and of two mutated alleles in Escherichia coli
journal, October 1992

A Novel Quinone-forming Monooxygenase Family Involved in Modification of Aromatic Polyketides
journal, February 2005

Bioengineering for Microbial Inulinases: Trends and Applications
journal, July 2017

The inhibition by substrate analogues of γ-aminobutyrate aminotransferase from mitochondria of different subcellular fractions of rat brain
journal, April 1977

Enoyl Coenzyme A Hydratase (Crotonase)
journal, August 1972

Structure of the EntB Multidomain Nonribosomal Peptide Synthetase and Functional Analysis of Its Interaction with the EntE Adenylation Domain
journal, April 2006

Development and evaluation of efficient recombinant Escherichia coli strains for the production of 3-hydroxypropionic acid from glycerol
journal, January 2009

Binding of Two Flaviolin Substrate Molecules, Oxidative Coupling, and Crystal Structure of Streptomyces coelicolor A3(2) Cytochrome P450 158A2
journal, January 2005

Genome-wide screening for trait conferring genes using DNA microarrays
journal, May 2002