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Title: Method for producing 3-hydroxypropionic acid and other products


This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes 3-hydroxypropionic acid.

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Issue Date:
Research Org.:
Cargill, Incorporated, Wayzata, MN (United States)
Sponsoring Org.:
OSTI Identifier:
Patent Number(s):
Application Number:
Cargill, Incorporated (Wayzata, MN)
DOE Contract Number:  
Resource Type:
Resource Relation:
Patent File Date: 2016 May 27
Country of Publication:
United States

Citation Formats

Lynch, Michael D., Gill, Ryan T., and Lipscomb, Tanya E. W. Method for producing 3-hydroxypropionic acid and other products. United States: N. p., 2018. Web.
Lynch, Michael D., Gill, Ryan T., & Lipscomb, Tanya E. W. Method for producing 3-hydroxypropionic acid and other products. United States.
Lynch, Michael D., Gill, Ryan T., and Lipscomb, Tanya E. W. Tue . "Method for producing 3-hydroxypropionic acid and other products". United States.
title = {Method for producing 3-hydroxypropionic acid and other products},
author = {Lynch, Michael D. and Gill, Ryan T. and Lipscomb, Tanya E. W.},
abstractNote = {This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes 3-hydroxypropionic acid.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {10}


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Works referenced in this record:

Method for purifying lactic acid
patent, December 2002

Engineering Central Metabolic Pathways for High-Level Flavonoid Production in Escherichia coli
journal, April 2007

  • Leonard, E.; Lim, K. -H.; Saw, P. -N.
  • Applied and Environmental Microbiology, Vol. 73, Issue 12
  • DOI: 10.1128/AEM.00200-07

Construction of an Escherichia coli K-12 Mutant for Homoethanologenic Fermentation of Glucose or Xylose without Foreign Genes
journal, January 2007

  • Kim, Y.; Ingram, L. O.; Shanmugam, K. T.
  • Applied and Environmental Microbiology, Vol. 73, Issue 6, p. 1766-1771
  • DOI: 10.1128/AEM.02456-06

Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design
journal, August 2005

  • Chica, Roberto A.; Doucet, Nicolas; Pelletier, Joelle N.
  • Current Opinion in Biotechnology, Vol. 16, Issue 4, p. 378-384
  • DOI: 10.1016/j.copbio.2005.06.004

Bioengineering for Microbial Inulinases: Trends and Applications
journal, July 2017

De novo biosynthetic pathways: rational design of microbial chemical factories
journal, October 2008

  • Prather, Kristala L. Jones; Martin, Collin H.
  • Current Opinion in Biotechnology, Vol. 19, Issue 5, p. 468-474
  • DOI: 10.1016/j.copbio.2008.07.009

Application of Functional Genomics to Pathway Optimization for Increased Isoprenoid Production
journal, March 2008

  • Kizer, L.; Pitera, D. J.; Pfleger, B. F.
  • Applied and Environmental Microbiology, Vol. 74, Issue 10
  • DOI: 10.1128/AEM.02750-07

Malonyl-Coenzyme A Reductase in the Modified 3-Hydroxypropionate Cycle for Autotrophic Carbon Fixation in Archaeal Metallosphaera and Sulfolobus spp.
journal, October 2006

  • Alber, B.; Olinger, M.; Rieder, A.
  • Journal of Bacteriology, Vol. 188, Issue 24, p. 8551-8559
  • DOI: 10.1128/JB.00987-06

Sequencing and overexpression of the Escherichia coli aroE gene encoding shikimate dehydrogenase
journal, January 1988

  • Anton, I. A.; Coggins, J. R.
  • Biochemical Journal, Vol. 249, Issue 2
  • DOI: 10.1042/bj2490319

A New Enzymatic Method of Acrylamide Production
journal, May 1982

Inverse metabolic engineering: A strategy for directed genetic engineering of useful phenotypes
journal, July 2002

  • Bailey, James E.; Sburlati, Adriana; Hatzimanikatis, Vassily
  • Biotechnology and Bioengineering, Vol. 79, Issue 5
  • DOI: 10.1002/bit.10441

Toward a science of metabolic engineering
journal, June 1991

Induction of specific base-pair substitutions in E. coli trpA mutants by chloroenthylene oxide, a carcinogenic vinyl chloride metabolite
journal, November 1985

  • Barbin, Alain; Besson, Francoise; Perrard, Marie-Hélène
  • Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 152, Issue 2-3, p. 147-156
  • DOI: 10.1016/0027-5107(85)90056-9

Engineered ketol-acid reductoisomerase and alcohol dehydrogenase enable anaerobic 2-methylpropan-1-ol production at theoretical yield in Escherichia coli
journal, May 2011

  • Bastian, Sabine; Liu, Xiang; Meyerowitz, Joseph T.
  • Metabolic Engineering, Vol. 13, Issue 3, p. 345-352
  • DOI: 10.1016/j.ymben.2011.02.004

The biological degradation of cellulose
journal, January 1994

Sequences of the envM gene and of two mutated alleles in Escherichia coli
journal, October 1992

Naturally Occurring Adenines within mRNA Coding Sequences Affect Ribosome Binding and Expression in Escherichia coli
journal, November 2006

  • Brock, J. E.; Paz, R. L.; Cottle, P.
  • Journal of Bacteriology, Vol. 189, Issue 2
  • DOI: 10.1128/JB.01356-06

Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids
journal, November 1998

Synthesis of labeled acrylamide andN-methylolacrylamide (NMA):15N-acrylamide,13C-NMA,15N-NMA, and13C,15N-NMA
journal, January 2005

  • Brown, N. R.; Frazier, C. E.
  • Journal of Labelled Compounds and Radiopharmaceuticals, Vol. 48, Issue 14
  • DOI: 10.1002/jlcr.1015

The IdhA Gene Encoding the Fermentative Lactate Dehydrogenase of Escherichia Coli
journal, January 1997

Directed Evolution of Toluene ortho-Monooxygenase for Enhanced 1-Naphthol Synthesis and Chlorinated Ethene Degradation
journal, January 2002

Simultaneous synthesis of enantiomerically pure (S)-amino acids and (R)-amines using coupled transaminase reactions
journal, January 2003

  • Cho, Byung-Kwan; Cho, Ho Jung; Park, Sung-Hee
  • Biotechnology and Bioengineering, Vol. 81, Issue 7
  • DOI: 10.1002/bit.10526

The commercial production of chemicals using pathway engineering
journal, December 2000

  • Chotani, Gopal; Dodge, Tim; Hsu, Amy
  • Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 1543, Issue 2
  • DOI: 10.1016/S0167-4838(00)00234-X

Inhibitory activity spectrum of reuterin produced by Lactobacillus reuteri against intestinal bacteria
journal, January 2007

  • Cleusix, Valentine; Lacroix, Christophe; Vollenweider, Sabine
  • BMC Microbiology, Vol. 7, Issue 1
  • DOI: 10.1186/1471-2180-7-101

Characterization of the major promoter for the plasmid-encoded sucrose genes scrY, scrA, and scrB.
journal, December 1991

DNA shuffling of a family of genes from diverse species accelerates directed evolution
journal, January 1998

  • Crameri, Andreas; Raillard, Sun-Ai; Bermudez, Ericka
  • Nature, Vol. 391, Issue 6664
  • DOI: 10.1038/34663

Cloning, crystallization and preliminary characterization of a β-carbonic anhydrase from Escherichia coli
journal, September 2000

  • Cronk, Jeff D.; O'Neill, Jason W.; Cronk, Michelle R.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 56, Issue 9, p. 1176-1179
  • DOI: 10.1107/S0907444900008519

Thermal regulation of membrane lipid fluidity in bacteria
journal, February 1983

Practical limits of function prediction
journal, January 2000

Engineering of a functional human NADH-dependent cytochrome P450 system
journal, January 2001

  • Dohr, O.; Paine, M. J. I.; Friedberg, T.
  • Proceedings of the National Academy of Sciences, Vol. 98, Issue 1
  • DOI: 10.1073/pnas.98.1.81

Structure of the EntB Multidomain Nonribosomal Peptide Synthetase and Functional Analysis of Its Interaction with the EntE Adenylation Domain
journal, April 2006

Lactate-Utilizing Bacteria, Isolated from Human Feces, That Produce Butyrate as a Major Fermentation Product
journal, October 2004

The overexpression and complete amino acid sequence of Escherichia coli 3-dehydroquinase
journal, September 1986

  • Duncan, K.; Chaudhuri, S.; Campbell, M. S.
  • Biochemical Journal, Vol. 238, Issue 2
  • DOI: 10.1042/bj2380475

Switch of coenzyme specificity of p -hydroxybenzoate hydroxylase 1 1Edited by A. R. Fersht
journal, September 1999

  • Eppink, Michel H. M.; Overkamp, Karin M.; Schreuder, Herman A.
  • Journal of Molecular Biology, Vol. 292, Issue 1
  • DOI: 10.1006/jmbi.1999.3015

Improving lycopene production in Escherichia coli by engineering metabolic control
journal, May 2000

  • Farmer, William R.; Liao, James C.
  • Nature Biotechnology, Vol. 18, Issue 5
  • DOI: 10.1038/75398

Biorefineries:  Current Status, Challenges, and Future Direction
journal, July 2006

  • Fernando, Sandun; Adhikari, Sushil; Chandrapal, Chauda
  • Energy & Fuels, Vol. 20, Issue 4, p. 1727-1737
  • DOI: 10.1021/ef060097w

Light-directed, spatially addressable parallel chemical synthesis
journal, February 1991

A Novel Quinone-forming Monooxygenase Family Involved in Modification of Aromatic Polyketides
journal, February 2005

  • Funa, Nobutaka; Funabashi, Masanori; Yoshimura, Etsuro
  • Journal of Biological Chemistry, Vol. 280, Issue 15
  • DOI: 10.1074/jbc.M500190200

Genome-wide screening for trait conferring genes using DNA microarrays
journal, May 2002

  • Gill, R. T.; Wildt, S.; Yang, Y. T.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 10, p. 7033-7038
  • DOI: 10.1073/pnas.102154799

Identification and cloning of the Mycobacterium avium folA gene, required for dihydrofolate reductase activity
journal, November 1997

Metabolic Analysis of Escherichia coli in the Presence and Absence of the Carboxylating Enzymes Phosphoenolpyruvate Carboxylase and Pyruvate Carboxylase
journal, May 2000

Overproduction of phage Lambda repressor under control of the lac promotor of Escherichia coli
journal, January 1976

  • Gronenborn, Bruno
  • MGG Molecular & General Genetics, Vol. 148, Issue 3
  • DOI: 10.1007/BF00332898

Genetic Evidence for an Interaction of the UbiG O-Methyltransferase with UbiX in Escherichia coli Coenzyme Q Biosynthesis
journal, August 2006

  • Gulmezian, M.; Zhang, H.; Javor, G. T.
  • Journal of Bacteriology, Vol. 188, Issue 17
  • DOI: 10.1128/JB.00668-06

Exploring the diversity of complex metabolic networks
journal, December 2004

A T42M Substitution in Bacterial 5-Enolpyruvylshikimate-3-phosphate Synthase (EPSPS) Generates Enzymes with Increased Resistance to Glyphosate
journal, January 2003

  • He, Ming; Nie, Yan-Fang; Xu, Peilin
  • Bioscience, Biotechnology, and Biochemistry, Vol. 67, Issue 6
  • DOI: 10.1271/bbb.67.1405

Enoyl-Acyl Carrier Protein Reductase ( fabI ) Plays a Determinant Role in Completing Cycles of Fatty Acid Elongation in Escherichia coli
journal, November 1995

Autotrophic CO2 Fixation by Chloroflexus aurantiacus: Study of Glyoxylate Formation and Assimilation via the 3-Hydroxypropionate Cycle
journal, July 2001

Oxidation of Cysteine 645 of Cobalamin-Independent Methionine Synthase Causes a Methionine Limitation in Escherichia coli
journal, March 2009

  • Hondorp, E. R.; Matthews, R. G.
  • Journal of Bacteriology, Vol. 191, Issue 10
  • DOI: 10.1128/JB.01722-08

Malonyl-Coenzyme A Reductase from Chloroflexus aurantiacus, a Key Enzyme of the 3-Hydroxypropionate Cycle for Autotrophic CO2 Fixation
journal, May 2002

Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis
journal, May 2003

  • Ivanova, Natalia; Sorokin, Alexei; Anderson, Iain
  • Nature, Vol. 423, Issue 6935, p. 87-91
  • DOI: 10.1038/nature01582

Biosynthetic pathways for 3-hydroxypropionic acid production
journal, February 2009

  • Jiang, Xinglin; Meng, Xin; Xian, Mo
  • Applied Microbiology and Biotechnology, Vol. 82, Issue 6
  • DOI: 10.1007/s00253-009-1898-7

Purification and characterization of 2-oxoglutarate decarboxylase of Leuconostoc oenos
journal, August 1990

Engineering primary metabolic pathways of industrial micro-organisms
journal, March 2007

Extractive recovery of products from fermentation broths
journal, April 1999

  • Kim, Joong Kyun; Iannotti, Eugene L.; Bajpai, Rakesh
  • Biotechnology and Bioprocess Engineering, Vol. 4, Issue 1
  • DOI: 10.1007/BF02931905

The Rut Pathway for Pyrimidine Degradation: Novel Chemistry and Toxicity Problems
journal, April 2010

  • Kim, K. -S.; Pelton, J. G.; Inwood, W. B.
  • Journal of Bacteriology, Vol. 192, Issue 16
  • DOI: 10.1128/JB.00201-10

Dihydrolipoamide Dehydrogenase Mutation Alters the NADH Sensitivity of Pyruvate Dehydrogenase Complex of Escherichia coli K-12
journal, March 2008

  • Kim, Y.; Ingram, L. O.; Shanmugam, K. T.
  • Journal of Bacteriology, Vol. 190, Issue 11, p. 3851-3858
  • DOI: 10.1128/JB.00104-08

Effect of Overexpression of Actinobacillus succinogenes Phosphoenolpyruvate Carboxykinase on Succinate Production in Escherichia coli
journal, February 2004

A "Silent" Polymorphism in the MDR1 Gene Changes Substrate Specificity
journal, January 2007

Polypeptide Release Factors in Prokaryotes and Eukaryotes
journal, January 2002

Expression of proteins encoded by the Escherichia coli cyn operon: carbon dioxide-enhanced degradation of carbonic anhydrase.
journal, September 1994

Role of bicarbonate/CO2 in the inhibition of Escherichia coli growth by cyanate.
journal, June 1995

Reactions of β-lactones with potassium alkoxides and their complexes with 18-crown-6 in aprotic solvents
journal, July 1993

  • Kurcok, Piotr; Jedlinski, Zbigniew; Kowalczuk, Marek
  • The Journal of Organic Chemistry, Vol. 58, Issue 16
  • DOI: 10.1021/jo00068a015

A Physiology Study of Escherichia coli Overexpressing Phosphoenolpyruvate Carboxykinase
journal, April 2008

  • Kwon, Yeong-Deok; Lee, Sang Yup; Kim, Pil
  • Bioscience, Biotechnology, and Biochemistry, Vol. 72, Issue 4
  • DOI: 10.1271/bbb.70831

A new preparation of trifluoromethanesulfinate salts
journal, July 2007

  • Langlois, Bernard R.; Billard, Thierry; Mulatier, Jean-Christophe
  • Journal of Fluorine Chemistry, Vol. 128, Issue 7, p. 851-856
  • DOI: 10.1016/j.jfluchem.2007.04.012

A process for microbial hydrocarbon synthesis: Overproduction of fatty acids in Escherichia coli and catalytic conversion to alkanes
journal, June 2010

  • Lennen, Rebecca M.; Braden, Drew J.; West, Ryan M.
  • Biotechnology and Bioengineering, Vol. 106, Issue 2, p. 193-202
  • DOI: 10.1002/bit.22660

Effect of poxB gene knockout on metabolism in Escherichia coli based on growth characteristics and enzyme activities
journal, September 2006

  • Li, Mai; Yao, Shanjing; Shimizu, Kazuyuki
  • World Journal of Microbiology and Biotechnology, Vol. 23, Issue 4
  • DOI: 10.1007/s11274-006-9267-5

Fe2(SO4)3·4H2O/concentrated H2SO4: an efficient catalyst for esterification
journal, March 2004

  • Liang, Yong-min; Xu, Qi-hai; Wu, Xiao-li
  • Journal of Chemical Research, Vol. 2004, Issue 3
  • DOI: 10.3184/0308234041640708

Feedback Inhibition of Chorismate Mutase/Prephenate Dehydrogenase (TyrA) of Escherichia coli: Generation and Characterization of Tyrosine-Insensitive Mutants
journal, November 2005

SCALEs: multiscale analysis of library enrichment
journal, November 2006

  • Lynch, Michael D.; Warnecke, Tanya; Gill, Ryan T.
  • Nature Methods, Vol. 4, Issue 1
  • DOI: 10.1038/nmeth946

Engineering a mevalonate pathway in Escherichia coli for production of terpenoids
journal, June 2003

  • Martin, Vincent J. J.; Pitera, Douglas J.; Withers, Sydnor T.
  • Nature Biotechnology, Vol. 21, Issue 7
  • DOI: 10.1038/nbt833

Aminotransferases: demonstration of homology and division into evolutionary subgroups
journal, June 1993

Nucleotide sequence of the Escherichia coli cad operon: a system for neutralization of low extracellular pH.
journal, April 1992

Effect of process parameters on 3-hydroxypropionic acid production from glycerol using a recombinant Escherichia coli
journal, April 2009

  • Mohan Raj, Subramanian; Rathnasingh, Chelladurai; Jung, Woo-Chel
  • Applied Microbiology and Biotechnology, Vol. 84, Issue 4
  • DOI: 10.1007/s00253-009-1986-8

The Lysine Decarboxylase CadA Protects Escherichia coli Starved of Phosphate against Fermentation Acids
journal, January 2007

The tyrosine repressor negatively regulates aroH expression in Escherichia coli.
journal, June 1991

Human Catechol-O-Methyltransferase Haplotypes Modulate Protein Expression by Altering mRNA Secondary Structure
journal, December 2006

  • Nackley, A. G.; Shabalina, S. A.; Tchivileva, I. E.
  • Science, Vol. 314, Issue 5807, p. 1930-1933
  • DOI: 10.1126/science.1131262

Structure of Cellulases and Their Applications
journal, April 1997

  • Ohmiya, Kunio; Sakka, Kazuo; Karita, Shuichi
  • Biotechnology and Genetic Engineering Reviews, Vol. 14, Issue 1, p. 365-414
  • DOI: 10.1080/02648725.1997.10647949

A novel methodology employing Corynebacterium glutamicum genome information to generate a new L -lysine-producing mutant
journal, January 2002

  • Ohnishi, S. Mitsuhashi, M. Hayashi,, J.
  • Applied Microbiology and Biotechnology, Vol. 58, Issue 2
  • DOI: 10.1007/s00253-001-0883-6

Unprecedented acetoacetyl-coenzyme A synthesizing enzyme of the thiolase superfamily involved in the mevalonate pathway
journal, June 2010

  • Okamura, E.; Tomita, T.; Sawa, R.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 25, p. 11265-11270
  • DOI: 10.1073/pnas.1000532107

Cloning and Overexpression in Soluble Form of Functional Shikimate Kinase and 5-Enolpyruvylshikimate 3-Phosphate Synthase Enzymes from Mycobacterium tuberculosis
journal, August 2001

  • Oliveira, Jaim S.; Pinto, Clotilde A.; Basso, Luiz A.
  • Protein Expression and Purification, Vol. 22, Issue 3
  • DOI: 10.1006/prep.2001.1457

Metabolic engineering of aromatic group amino acid pathway in Bacillus subtilis for L-phenylalanine production
journal, November 2004

  • Özçelik, İlknur Şenver; Çalık, Pinar; Çalık, Güzide
  • Chemical Engineering Science, Vol. 59, Issue 22-23, p. 5019-5026
  • DOI: 10.1016/j.ces.2004.07.108

Directed evolution of RuBisCO hypermorphs through genetic selection in engineered E.coli
journal, January 2006

  • Parikh, Monal R.; Greene, Dina N.; Woods, Kristen K.
  • Protein Engineering, Design and Selection, Vol. 19, Issue 3, p. 113-119
  • DOI: 10.1093/protein/gzj010

Genome shuffling of Lactobacillus for improved acid tolerance
journal, July 2002

  • Patnaik, Ranjan; Louie, Susan; Gavrilovic, Vesna
  • Nature Biotechnology, Vol. 20, Issue 7
  • DOI: 10.1038/nbt0702-707

A new perspective on thiamine catalysis
journal, August 2004

Polyphosphate Kinase Protects Salmonella enterica from Weak Organic Acid Stress
journal, April 2005

A mild and efficient method for esterification and transesterification catalyzed by iodine
journal, January 2002

Mutational analysis of the catalytic and feedback sites of the tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase of Escherichia coli.
journal, December 1988

Molecular Iodine in Ionic Liquid: A Green Catalytic System for Esterification and Transesterification
journal, May 2010

Structure−Cytoprotective Activity Relationship of Simple Molecules Containing an α,β-Unsaturated Carbonyl System
journal, June 1997

  • Rodríguez, Ana M.; Enriz, Ricardo D.; Santagata, Luis N.
  • Journal of Medicinal Chemistry, Vol. 40, Issue 12
  • DOI: 10.1021/jm960280m

The catabolite repressor/activator (Cra) protein of enteric bacteria.
journal, June 1996

Silent Polymorphisms Speak: How They Affect Pharmacogenomics and the Treatment of Cancer
journal, October 2007

Molecular breeding of carotenoid biosynthetic pathways
journal, July 2000

  • Schmidt-Dannert, Claudia; Umeno, Daisuke; Arnold, Frances H.
  • Nature Biotechnology, Vol. 18, Issue 7
  • DOI: 10.1038/77319

Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different
journal, April 2001

Developments in Directed Evolution for Improving Enzyme Functions
journal, August 2007

  • Sen, S.; Venkata Dasu, V.; Mandal, B.
  • Applied Biochemistry and Biotechnology, Vol. 143, Issue 3, p. 212-223
  • DOI: 10.1007/s12010-007-8003-4

CHEMISTRY: Sugary Recipe Boosts Grow-Your-Own Plastics
journal, June 2006

Genes restoring redox balance in fermentation-deficient E. coli NZN111
journal, November 2009

  • Singh, Amarjeet; Lynch, Michael D.; Gill, Ryan T.
  • Metabolic Engineering, Vol. 11, Issue 6, p. 347-354
  • DOI: 10.1016/j.ymben.2009.07.002

The ARO4 gene of Candida albicans encodes a tyrosine-sensitive DAHP synthase: evolution, functional conservation and phenotype of Aro3p-, Aro4p-deficient mutants
journal, May 2002

Network rigidity and metabolic engineering in metabolite overproduction
journal, June 1991

Challenges in Engineering Microbes for Biofuels Production
journal, February 2007

Nucleotide sequence of the adi gene, which encodes the biodegradative acid-induced arginine decarboxylase of Escherichia coli.
journal, March 1993

Feasibility of acrylic acid production by fermentation
journal, February 2005

  • Straathof, Adrie J. J.; Sie, Susana; Franco, Telma T.
  • Applied Microbiology and Biotechnology, Vol. 67, Issue 6
  • DOI: 10.1007/s00253-005-1942-1

Enzymes of a novel autotrophic CO2 fixation pathway in the phototrophic bacterium Chloroflexus aurantiacus, the 3-hydroxypropionate cycle
journal, August 1993

?-hydroxypropionic acid production by Byssochlamys sp. grown on acrylic acid
journal, November 1993

  • Takamizawa, Kazuhiro; Horitsu, Hiroyuki; Ichikawa, Toshio
  • Applied Microbiology and Biotechnology, Vol. 40, Issue 2-3
  • DOI: 10.1007/BF00170365

Variant tricarboxylic acid cycle in Mycobacterium tuberculosis: Identification of α-ketoglutarate decarboxylase
journal, July 2005

  • Tian, J.; Bryk, R.; Itoh, M.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 30, p. 10670-10675
  • DOI: 10.1073/pnas.0501605102

The inhibition by substrate analogues of γ-aminobutyrate aminotransferase from mitochondria of different subcellular fractions of rat brain
journal, April 1977

  • Tunnicliff, G.; Ngo, T. T.; Rojo-Ortega, J. M.
  • Canadian Journal of Biochemistry, Vol. 55, Issue 4, p. 479-484
  • DOI: 10.1139/o77-067

3-phenylpropionate catabolism and the Escherichia coli oxidative stress response
journal, April 2005

  • Turlin, Evelyne; Sismeiro, Odile; Le Caer, Jean Pierre
  • Research in Microbiology, Vol. 156, Issue 3, p. 312-321
  • DOI: 10.1016/j.resmic.2004.10.012

Characterization of Mutations Contributing to Sulfathiazole Resistance in Escherichia coli
journal, January 1998

  • Vedantam, Gayatri; Guay, Gordon G.; Austria, Natasha E.
  • Antimicrobial Agents and Chemotherapy, Vol. 42, Issue 1, p. 88-93
  • DOI: 10.1128/AAC.42.1.88

A genomics approach to improve the analysis and design of strain selections
journal, May 2008

  • Warnecke, T. E.; Lynch, M. D.; Karimpour-Fard, A.
  • Metabolic Engineering, Vol. 10, Issue 3-4, p. 154-165
  • DOI: 10.1016/j.ymben.2008.04.004

Identification of a 21 amino acid peptide conferring 3-hydroxypropionic acid stress-tolerance to Escherichia coli
journal, January 2012

  • Warnecke, T. E.; Lynch, M. D.; Lipscomb, M. L.
  • Biotechnology and Bioengineering, Vol. 109, Issue 5, p. 1347-1352
  • DOI: 10.1002/bit.24398

Rapid dissection of a complex phenotype through genomic-scale mapping of fitness altering genes
journal, May 2010

  • Warnecke, T. E.; Lynch, M. D.; Karimpour-Fard, A.
  • Metabolic Engineering, Vol. 12, Issue 3, p. 241-250
  • DOI: 10.1016/j.ymben.2009.12.002

Fermentation of Glucose to Lactic Acid Coupled with Reactive Extraction:  A Review
journal, September 2004

  • Wasewar, Kailas L.; Yawalkar, Archis A.; Moulijn, Jacob A.
  • Industrial & Engineering Chemistry Research, Vol. 43, Issue 19
  • DOI: 10.1021/ie049963n

Prediction of protein function from protein sequence and structure
journal, January 1999

  • Whisstock, James C.; Lesk, Arthur M.
  • Quarterly Reviews of Biophysics, Vol. 36, Issue 3, p. 307-340
  • DOI: 10.1017/S0033583503003901

A Single Mutation Converts a Novel Phosphotyrosine Binding Domain into a Dual-specificity Phosphatase
journal, November 1995

  • Wishart, Matthew J.; Denu, John M.; Williams, John A.
  • Journal of Biological Chemistry, Vol. 270, Issue 45, p. 26782-26785
  • DOI: 10.1074/jbc.270.45.26782

Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine
journal, September 1999

  • Witkowski, Andrzej; Joshi, Anil K.; Lindqvist, Ylva
  • Biochemistry, Vol. 38, Issue 36, p. 11643-11650
  • DOI: 10.1021/bi990993h

On the Role of Helix 0 of the Tryptophan Synthetase α Chain of Escherichia coli
journal, June 1996

  • Yee, Muh Ching; Horn, Virginia; Yanofsky, Charles
  • Journal of Biological Chemistry, Vol. 271, Issue 25
  • DOI: 10.1074/jbc.271.25.14754

Identification of PhoB binding sites of the yibD and ytfK promoter regions in Escherichia coli
journal, April 2011

  • Yoshida, Yusuke; Sugiyama, Shinichiro; Oyamada, Tomoya
  • The Journal of Microbiology, Vol. 49, Issue 2
  • DOI: 10.1007/s12275-011-0360-6

Improving cellular malonyl-CoA level in Escherichia coli via metabolic engineering
journal, May 2009

  • Zha, Wenjuan; Rubin-Pitel, Sheryl B.; Shao, Zengyi
  • Metabolic Engineering, Vol. 11, Issue 3, p. 192-198
  • DOI: 10.1016/j.ymben.2009.01.005

Binding of Two Flaviolin Substrate Molecules, Oxidative Coupling, and Crystal Structure of Streptomyces coelicolor A3(2) Cytochrome P450 158A2
journal, January 2005

  • Zhao, B.; Guengerich, F. P.; Bellamine, A.
  • Journal of Biological Chemistry, Vol. 280, Issue 12
  • DOI: 10.1074/jbc.M410933200

Interdomain Communication between the Thiolation and Thioesterase Domains of EntF Explored by Combinatorial Mutagenesis and Selection
journal, August 2006

Control Mechanisms in the Synthesis of Saturated Fatty Acids
journal, June 1977

A core catalytic domain of the TyrA protein family: arogenate dehydrogenase from Synechocystis
journal, August 2004

  • Bonner, Carol A.; Jensen, Roy A.; Gander, John E.
  • Biochemical Journal, Vol. 382, Issue 1
  • DOI: 10.1042/BJ20031809

Selective production of L-aspartic acid and L-phenylalanine by coupling reactions of aspartase and aminotransferase in Escherichia coli
journal, July 2000

Menaquinone (vitamin K2) biosynthesis: overexpression, purification, and characterization of a new isochorismate synthase from Escherichia coli.
journal, May 1997

FolM, A New Chromosomally Encoded Dihydrofolate Reductase in Escherichia coli
journal, November 2003

The qmeA (ts) mutation of Escherichia coli is localized in the fabI gene, which encodes enoyl-ACP reductase
journal, January 1996

Characterization of two temperature-inducible promoters newly isolated from B. subtilis
journal, July 2007

  • Li, Wang; Li, Heng-Xin; Ji, Sheng-Yue
  • Biochemical and Biophysical Research Communications, Vol. 358, Issue 4, p. 1148-1153
  • DOI: 10.1016/j.bbrc.2007.05.064

Cloning and sequencing of Escherichia coli ubiC and purification of chorismate lyase.
journal, August 1992

Sequence and overexpression of the menD gene from Escherichia coli.
journal, August 1989

Overexpression, Purification, and Characterization of Tyrosine-Sensitive 3-Deoxy-d-arabino-heptulosonic Acid 7-Phosphate Synthase fromEscherichia coli
journal, March 1997

  • Ramilo, Cecilia A.; Evans, Jeremy N. S.
  • Protein Expression and Purification, Vol. 9, Issue 2
  • DOI: 10.1006/prep.1996.0690

Development and evaluation of efficient recombinant Escherichia coli strains for the production of 3-hydroxypropionic acid from glycerol
journal, January 2009

  • Rathnasingh, Chelladurai; Raj, Subramanian Mohan; Jo, Ji-Eun
  • Biotechnology and Bioengineering, Vol. 104, Issue 4, p. 729-739
  • DOI: 10.1002/bit.22429

Inhibition of Escherichia coli growth by acetic acid: a problem with methionine biosynthesis and homocysteine toxicity
journal, July 2002

Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli
journal, December 2002

  • Welch, R. A.; Burland, V.; Plunkett, G.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 26, p. 17020-17024
  • DOI: 10.1073/pnas.252529799

Inhibiting Bacterial Fatty Acid Synthesis
journal, April 2006

  • Zhang, Yong-Mei; White, Stephen W.; Rock, Charles O.
  • Journal of Biological Chemistry, Vol. 281, Issue 26
  • DOI: 10.1074/jbc.R600004200