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Title: Gene-matched enrichment and polymerase chain reaction for rapid detection of microorganisms

Abstract

A method for amplifying and detecting microorganisms, such as species of Listeria, is described. The method utilizes gene-matched enrichment media and PCR-based detection. The enrichment media is spent media produced using a modified microorganism containing a plurality of mutations in a selected gene such that the modified microorganism does not contain the PCR signature. Thus, PCR detects only the amplified microorganism of interest, not the modified microorganism. Exemplary methods and kits for amplification and detection of Listeria species are described.

Inventors:
; ; ;
Issue Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1489680
Patent Number(s):
10,100,369
Application Number:
14/742,367
Assignee:
Battelle Memorial Institute (Richland, WA)
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015 Jun 17
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Ozanich, Richard M., Hutchison, Janine R., Victry, Kristin D., and Hess, Becky M. Gene-matched enrichment and polymerase chain reaction for rapid detection of microorganisms. United States: N. p., 2018. Web.
Ozanich, Richard M., Hutchison, Janine R., Victry, Kristin D., & Hess, Becky M. Gene-matched enrichment and polymerase chain reaction for rapid detection of microorganisms. United States.
Ozanich, Richard M., Hutchison, Janine R., Victry, Kristin D., and Hess, Becky M. Tue . "Gene-matched enrichment and polymerase chain reaction for rapid detection of microorganisms". United States. https://www.osti.gov/servlets/purl/1489680.
@article{osti_1489680,
title = {Gene-matched enrichment and polymerase chain reaction for rapid detection of microorganisms},
author = {Ozanich, Richard M. and Hutchison, Janine R. and Victry, Kristin D. and Hess, Becky M.},
abstractNote = {A method for amplifying and detecting microorganisms, such as species of Listeria, is described. The method utilizes gene-matched enrichment media and PCR-based detection. The enrichment media is spent media produced using a modified microorganism containing a plurality of mutations in a selected gene such that the modified microorganism does not contain the PCR signature. Thus, PCR detects only the amplified microorganism of interest, not the modified microorganism. Exemplary methods and kits for amplification and detection of Listeria species are described.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {10}
}

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Works referenced in this record:

Association of rpoB mutations with rifampicin resistance in Mycobacterium avium
journal, January 2006


Differential expression of InlB and ActA in Listeria monocytogenes in selective and nonselective enrichment broths
journal, March 2008


Starvation survival in Listeria monocytogenes: characterization of the response and the role of known and novel components
journal, August 2001


Assessment of the pathogenic potential of two Listeria monocytogenes human faecal carriage isolates
journal, June 2002

  • Rousset, André; Divies, Charles; Lemaı̂tre, Jean-Paul
  • Microbiology, Vol. 148, Issue 6, p. 1855-1862
  • DOI: 10.1099/00221287-148-6-1855

Multiple point mutations in virulence genes explain the low virulence of Listeria monocytogenes field strains
journal, March 2008


SEL, a Selective Enrichment Broth for Simultaneous Growth of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes
journal, June 2008

  • Kim, H.; Bhunia, A. K.
  • Applied and Environmental Microbiology, Vol. 74, Issue 15, p. 4853-4866
  • DOI: 10.1128/AEM.02756-07

Catellibacterium nectariphilum gen. nov., sp. nov., which requires a diffusible compound from a strain related to the genus Sphingomonas for vigorous growth
journal, May 2004

  • Tanaka, Yasuhiro; Hanada, Satoshi; Manome, Akira
  • International Journal of Systematic and Evolutionary Microbiology, Vol. 54, Issue 3, p. 955-959
  • DOI: 10.1099/ijs.0.02750-0

Strategies for culture of ‘unculturable’ bacteria: Culturing the unculturable
journal, April 2010


QUORUM SENSING: Cell-to-Cell Communication in Bacteria
journal, November 2005


Conditioned Medium from Listeria innocua Stimulates Emergence from a Resting State: Not a Response to E. coli Quorum Sensing Autoinducer AI-2
journal, April 2006

  • Yang, L.; Portugal, F.; Bentley, W. E.
  • Biotechnology Progress, Vol. 22, Issue 2, p. 387-393
  • DOI: 10.1021/bp050282c