Title: Gene-matched enrichment and polymerase chain reaction for rapid detection of microorganisms

Abstract

A method for amplifying and detecting microorganisms, such as species of Listeria, is described. The method utilizes gene-matched enrichment media and PCR-based detection. The enrichment media is spent media produced using a modified microorganism containing a plurality of mutations in a selected gene such that the modified microorganism does not contain the PCR signature. Thus, PCR detects only the amplified microorganism of interest, not the modified microorganism. Exemplary methods and kits for amplification and detection of Listeria species are described.

Inventors:

Ozanich, Richard M.; Hutchison, Janine R.; Victry, Kristin D.; Hess, Becky M.

Issue Date:

Tue Oct 16 00:00:00 EDT 2018

Research Org.:

Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1489680

Patent Number(s):

10100369

Application Number:

14/742,367

Assignee:

Battelle Memorial Institute (Richland, WA)

Patent Classifications (CPCs):

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K14/195 - from bacteria

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N1/20 - Bacteria {

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/689 - for bacteria

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DOE Contract Number:  

AC05-76RL01830

Resource Type:

Patent

Resource Relation:

Patent File Date: 2015 Jun 17

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES