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Title: Gene-matched enrichment and polymerase chain reaction for rapid detection of microorganisms

A method for amplifying and detecting microorganisms, such as species of Listeria, is described. The method utilizes gene-matched enrichment media and PCR-based detection. The enrichment media is spent media produced using a modified microorganism containing a plurality of mutations in a selected gene such that the modified microorganism does not contain the PCR signature. Thus, PCR detects only the amplified microorganism of interest, not the modified microorganism. Exemplary methods and kits for amplification and detection of Listeria species are described.
Inventors:
; ; ;
Issue Date:
OSTI Identifier:
1489680
Assignee:
Battelle Memorial Institute (Richland, WA) PNNL
Patent Number(s):
10,100,369
Application Number:
14/742,367
Contract Number:
AC05-76RL01830
Resource Relation:
Patent File Date: 2015 Jun 17
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES