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Title: Gene-matched enrichment and polymerase chain reaction for rapid detection of microorganisms

Abstract

A method for amplifying and detecting microorganisms, such as species of Listeria, is described. The method utilizes gene-matched enrichment media and PCR-based detection. The enrichment media is spent media produced using a modified microorganism containing a plurality of mutations in a selected gene such that the modified microorganism does not contain the PCR signature. Thus, PCR detects only the amplified microorganism of interest, not the modified microorganism. Exemplary methods and kits for amplification and detection of Listeria species are described.

Inventors:
; ; ;
Issue Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1489680
Patent Number(s):
10100369
Application Number:
14/742,367
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12R - PROCESSES USING MICROORGANISMS
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015 Jun 17
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Ozanich, Richard M., Hutchison, Janine R., Victry, Kristin D., and Hess, Becky M. Gene-matched enrichment and polymerase chain reaction for rapid detection of microorganisms. United States: N. p., 2018. Web.
Ozanich, Richard M., Hutchison, Janine R., Victry, Kristin D., & Hess, Becky M. Gene-matched enrichment and polymerase chain reaction for rapid detection of microorganisms. United States.
Ozanich, Richard M., Hutchison, Janine R., Victry, Kristin D., and Hess, Becky M. Tue . "Gene-matched enrichment and polymerase chain reaction for rapid detection of microorganisms". United States. https://www.osti.gov/servlets/purl/1489680.
@article{osti_1489680,
title = {Gene-matched enrichment and polymerase chain reaction for rapid detection of microorganisms},
author = {Ozanich, Richard M. and Hutchison, Janine R. and Victry, Kristin D. and Hess, Becky M.},
abstractNote = {A method for amplifying and detecting microorganisms, such as species of Listeria, is described. The method utilizes gene-matched enrichment media and PCR-based detection. The enrichment media is spent media produced using a modified microorganism containing a plurality of mutations in a selected gene such that the modified microorganism does not contain the PCR signature. Thus, PCR detects only the amplified microorganism of interest, not the modified microorganism. Exemplary methods and kits for amplification and detection of Listeria species are described.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {10}
}

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Works referenced in this record:

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