Chemical amplification based on fluid partitioning
Abstract
A system for nucleic acid amplification of a sample comprises partitioning the sample into partitioned sections and performing PCR on the partitioned sections of the sample. Another embodiment of the invention provides a system for nucleic acid amplification and detection of a sample comprising partitioning the sample into partitioned sections, performing PCR on the partitioned sections of the sample, and detecting and analyzing the partitioned sections of the sample.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1485053
- Patent Number(s):
- RE47,080
- Application Number:
- 15/421,141
- Assignee:
- Lawrence Livermore National Security, LLC (Livermore, CA)
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2017 Jan 31
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Anderson, Brian L., Colston, Bill W., and Elkin, Christopher J. Chemical amplification based on fluid partitioning. United States: N. p., 2018.
Web.
Anderson, Brian L., Colston, Bill W., & Elkin, Christopher J. Chemical amplification based on fluid partitioning. United States.
Anderson, Brian L., Colston, Bill W., and Elkin, Christopher J. Tue .
"Chemical amplification based on fluid partitioning". United States. https://www.osti.gov/servlets/purl/1485053.
@article{osti_1485053,
title = {Chemical amplification based on fluid partitioning},
author = {Anderson, Brian L. and Colston, Bill W. and Elkin, Christopher J.},
abstractNote = {A system for nucleic acid amplification of a sample comprises partitioning the sample into partitioned sections and performing PCR on the partitioned sections of the sample. Another embodiment of the invention provides a system for nucleic acid amplification and detection of a sample comprising partitioning the sample into partitioned sections, performing PCR on the partitioned sections of the sample, and detecting and analyzing the partitioned sections of the sample.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {10}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Method and Device for Detecting the Presence of a Single Target Nucleic Acid in Samples
patent-application, September 2008
- Brown, James F.
- US Patent Application 11/837581; 20080213766
Methods of screening for lung neoplasm based on stool samples containing a nucleic acid marker indicative of a neoplasm
patent, May 2008
- Shuber, Anthony P.; Ahlquist, David A.
- US Patent Document 7,368,233
Method for detecting the presence of a single target nucleic acid in a sample
patent, July 2011
- Brown, James F.; Silver, Jonathan E.
- US Patent Document 7,972,778
Method for chemical amplification based on fluid partitioning in an immiscible liquid
patent, February 2017
- Anderson, Brian L.; Colston, Bill W.; Elkin, Christopher J.
- US Patent Document RE46,322
Microfluidic devices for the controlled manipulation of small volumes
patent, February 2003
- Ramsey, J. Michael; Jacobson, Stephen C.
- US Patent Document 6,524,456
Single molecule amplification and detection of DNA
patent-application, November 2004
- Knapp, Michael R.; Baker, Jill M.; Chow, Andrew W.
- US Patent Application 10/741162; 20040224325
Optical detection of dental disease using polarized light
patent-application, July 2002
- Everett, Matthew J.; Colston, JR., Billy W.; Sathyam, Ujwal S.
- US Patent Application 10/100824; 20020093655
Methods and systems for releasing intracellular material from cells within microfluidic samples of fluids
patent, March 2007
- Wu, Betty; Ganesan, Karthik; Handique, Kalyan
- US Patent Document 7,192,557
Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture
patent-application, July 2008
- Beer, Neil; Hindson, Benjamin; Colson, Billy
- US Patent Application 11/650363; 20080166793
Chemical amplification based on fluid partitioning in an immiscible liquid
patent, September 2010
- Anderson, Brian L.; Colston, Bill W.; Elkin, Christopher J.
- US Patent Document RE41,780
A microfluidic droplet generator based on a piezoelectric actuator
journal, January 2009
- Bransky, Avishay; Korin, Natanel; Khoury, Maria
- Lab Chip, Vol. 9, Issue 4, p. 516-520
Microfluidic devices for electrophoretic analysis of materials
patent, January 2002
- Parce, J. Wallace
- US Patent Document 6,337,740
Micromachined magnetohydrodynamic actuators and sensors
patent, November 2000
- Lee, Abraham P.; Lemoff, Asuncion V.
- US Patent Document 6,146,103
In Vitro sorting method
patent, August 2007
- Griffiths, Andrew David; Tawfik, Dan
- US Patent Document 7,252,943
Method for Quantifying Number of Molecules of Target Nucleic Acid Contained in a Sample
patent-application, July 2008
- Brown, James F.; Silver, Jonathan E.
- US Patent Application 11/837569; 20080171324
PCR and hybridization methods utilizing electrostatic transportation and devices therefor
patent-application, August 2006
- Higuchi, Toshiro; Torii, Toru; Taniguchi, Tomohiro
- US Patent Application 11/392505; 20060172336
PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets
journal, September 2005
- Kojima, Takaaki; Takei, Yoshiaki; Ohtsuka, Miharu
- Nucleic Acids Research, Vol. 33, Issue 17, p. e150-e150
Device and method for pressure-driven plug transport and reaction
patent, October 2006
- Ismagilov, Rustem F.; Tice, Joshua David; Song, Helen
- US Patent Document 7,129,091
Microfluidic devices for controlled viscous shearing and formation of amphiphilic vesicles
patent, September 2009
- Lee, Abraham P.; Tan, Yung-Chieh
- US Patent Document 7,595,195
Sigmoidal curve-fitting redefines quantitative real-time PCR with the prospective of developing automated high-throughput applications
journal, January 2004
- Rutledge, R. G.
- Nucleic Acids Research, Vol. 32, Issue 22, p. e178-e178
Method and Device for Detecting the Presence of a Single Target Nucleic Acid in a Sample
patent-application, July 2008
- Brown, James F.; Silver, Jonathan E.
- US Patent Application 11/837620; 20080171325
Method for creating chemical sensors using contact-based microdispensing technology
patent-application, October 2005
- Carter, J. Chance; Colston, Billy W. JR.; Brown, Steve B.
- US Patent Application 11/099274; 20050221279
Mesoscale polynucleotide amplification devices
patent, December 1996
- Wilding, Peter; Kricka, Larry J.
- US Patent Document 5,587,128
Microfluidic systems and methods of genotyping
patent, June 2002
- Knapp, Michael R.; Parce, John Wallace; Bousse, Luc
- US Patent Document 6,403,338
Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
patent, December 1996
- Zanzucchi, Peter John; Cherukuri, Satyam Choudary; McBride, Sterling Edward
- US Patent Document 5,585,069
Variable flexure-based fluid filter
patent, March 2007
- Brown, Steve B.; Colston, JR., Billy W.; Marshall, Graham
- US Patent Document 7,188,731
Microfluidic structure and process for its manufacture
patent, December 1994
- Ekstrom, Bjorn; Jacobson, Gunilla; Ohman, Ove
- US Patent Document 5,376,252
Magnetic levitation stirring devices and machines for mixing in vessels
patent, March 2002
- Cleveland, Patrick H.; Markle, John R.
- US Patent Document 6,357,907
Moving microdroplets
patent, October 2000
- Handique, Kalyan; Gogoi, Bishnu Prasanna; Burns, Mark A.
- US Patent Document 6,130,098
Highly parallel genomic assays
journal, August 2006
- Fan, Jian-Bing; Chee, Mark S.; Gunderson, Kevin L.
- Nature Reviews Genetics, Vol. 7, Issue 8, p. 632-644
X-ray compass for determining device orientation
patent, June 1999
- Da Silva, Luiz B.; Matthews, Dennis L.; Fitch, Joseph P.
- US Patent Document 5,912,945
Method for detecting the presence of a single target nucleic acid in a sample
patent, September 2012
- Brown, James F.; Silver, Jonathan E.
- US Patent Document 8,257,925
Device and method for the automated cycling of solutions between two or more temperatures
patent, December 1993
- Corbett, John; Reed, Kenneth Todd; Riggs, Arthur D.
- US Patent Document 5,270,183
Nucleic acid amplification reaction apparatus
patent, October 1998
- Haff, Lawrence A.; Picozza, Enrico; Bloch, Will
- US Patent Document 5,827,480
Microfluidic system and methods of use
patent, May 2005
- Harrison, Daniel Jed; Andersson, Per-Erik; Li, Paul
- US Patent Document 6,900,021
Formation of droplets using branch channels in a microfluidic circuit
conference, January 2002
- Nisisako, T.; Torii, T.; Higuchi, T.
- Proceedings of the 41st SICE Annual Conference
Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
patent, February 1997
- Atwood, John G.; Mossa, Albert Carmelo; Goven, Lisa May
- US Patent Document 5,602,756
Nanoliter scale PCR with TaqMan detection
journal, May 1997
- Kalinina, Olga; Lebedeva, Irina; Brown, James
- Nucleic Acids Research, Vol. 25, Issue 10, p. 1999-2004
Method and apparatus for analysis and sorting of polynucleotides based on size
patent, April 2001
- Quake, Stephen R.; Volkmuth, Wayne
- US Patent Document 6,221,654
Optical fiber head for providing lateral viewing
patent, October 2002
- Everett, Matthew J.; Colston, JR., Billy W.; James, Dale L.
- US Patent Document 6,466,713
Microfluidic particle-analysis systems
patent, December 2007
- Chou, Hou-Pu; Daridon, Antoine; Farrell, Kevin
- US Patent Document 7,312,085
High-Throughput Quantitative Polymerase Chain Reaction in Picoliter Droplets
journal, December 2008
- Kiss, Margaret Macris; Ortoleva-Donnelly, Lori; Beer, N. Reginald
- Analytical Chemistry, Vol. 80, Issue 23, p. 8975-8981
Capillary flow device
patent, August 1990
- Hillman, Robert S.; Cobb, Michael E.; Allen, Jimmy D.
- US Patent Document 4,948,961
Methods for detecting and sorting polynucleotides based on size
patent, December 2004
- Quake, Stephen R.; Chou, Hou-Pu
- US Patent Document 6,833,242
Optical coherence domain reflectometry guidewire
patent, January 2001
- Colston, JR., Billy W.; Everett, Matthew J.; Da Silva, Luiz B.
- US Patent Document 6,175,669
Integrated systems and methods for diversity generation and screening
patent-application, November 2001
- Bass, Steven H.; Davis, S. Christopher; Patten, Phillip A.
- US Patent Application 09/760010; 20010039014
Methods for detecting nucleic acids indicative of cancer
patent, November 2005
- Shuber, Anthony P.
- US Patent Document 6,964,846
Method for conducting PCR protected from evaporation
patent, December 2003
- Sato, Yoshihiro
- US Patent Document 6,664,044
Device and method for the detection of an analyte utilizing mesoscale flow systems
patent, April 2003
- Wilding, Peter; Kricka, Larry J.; Zemel, Jay N.
- US Patent Document 6,551,841
Cell flow apparatus and method for real-time measurements of patient cellular responses
patent, May 2003
- Veerapandian, Pandi; Kaler, Gregory
- US Patent Document 6,558,916
Surface coating for microfluidic devices that incorporate a biopolymer resistant moiety
patent, December 2003
- Yang, Hua; Sundberg, Steven A.
- US Patent Document 6,660,367
Method for the complete chemical synthesis and assembly of genes and genomes
patent, February 2003
- Evans, Glen A.
- US Patent Document 6,521,427
Digital quantification using amplified single-molecule detection
journal, August 2006
- Jarvius, Jonas; Melin, Jonas; Göransson, Jenny
- Nature Methods, Vol. 3, Issue 9
Microscale devices and reactions in microscale devices
patent, May 2000
- Burns, Mark A.; Mastrangelo, Carlos H.; Sammarco, Timothy S.
- US Patent Document 6,057,149
Apparatus for packaging a chip
patent, August 1999
- Besemer, Donald; Goss, Virginia; Winkler, James L.
- US Patent Document 5,945,334
Microfabricated crossflow devices and methods
patent-application, May 2002
- Quake, Stephen R.; Thorsen, Todd
- US Patent Application 09/953103; 20020058332
Single molecule amplification and detection of DNA length
patent-application, February 2005
- Knapp, Michael R.; Baker, Jill M.; Chow, Andrea W.
- US Patent Application 10/845996; 20050042639
Emulsion compositions
patent-application, March 2005
- Holliger, Phillip; Ghadessy, Farid
- US Patent Application 10/866392; 20050064460
Portable pathogen detection system
patent-application, February 2003
- Colston, Billy W.; Everett, Matthew; Milanovich, Fred P.
- US Patent Application 10/260066; 20030027244
Multi-channel microfluidic system design with balanced fluid flow distribution
patent, October 2003
- Lei, Ming; Adey, Nils B.; McNeely, Michael R.
- US Patent Document 6,637,463
System for autonomous monitoring of bioagents
patent-application, February 2004
- Langlois, Richard G.; Milanovich, Fred P.; Colston, JR., Billy W.
- US Patent Application 10/643797; 20040038385
Hybrid automated continuous nucleic acid and protein analyzer using real-time PCR and liquid bead arrays
patent-application, October 2005
- Nasarabadi, Shanavaz L.; Langlois, Richard G.; Colston, JR., Billy W.
- US Patent Application 11/159008; 20050239192
The Contribution of Combinatorial Chemistry to Lead Generation An Interim Analysis
journal, July 2001
- Adang, Anton; Hermkens, Pedro
- Current Medicinal Chemistry, Vol. 8, Issue 9
Optical detection dental disease using polarized light
patent, February 2003
- Everett, Matthew J.; Colston, JR., Billy W.; Sathyam, Ujwal S.
- US Patent Document 6,522,407
Method and Device for Detecting the Presence of Target Nucleic Acids in a Sample, and Microfluidic Device for Use in Such Methods
patent-application, June 2008
- Brown, James F.; Siver, Jonathan E.
- US Patent Application 11/837613; 20080153091
Portable pathogen detection system
patent-application, January 2003
- Colston, Billy W.; Everett, Matthew; Milanovich, Fred P.
- US Patent Application 09/880515; 20030003441
Droplet-based synthetic method using microflow focusing and droplet fusion
journal, September 2006
- Liu, Kan; Ding, Huijiang; Chen, Yong
- Microfluidics and Nanofluidics, Vol. 3, Issue 2, p. 239-243
Integrated active flux microfluidic devices and methods
patent, July 2004
- Quake, Stephen R.; Chou, Hou-Pu
- US Patent Document 6,767,706
Apparatus for chemical amplification based on fluid partitioning in an immiscible liquid
patent, May 2012
- Anderson, Brian L.; Colston, Bill W.; Elkin, Christopher J.
- US Patent Document RE43,365
Dual resonance energy transfer nucleic acid probes
patent, July 2006
- Bao, Gang; Tsourkas, Andrew; Xu, Yangqing
- US Patent Document 7,081,336
Heat integration in micro-fluidic devices
book, January 2006
- Zhelev, Toshko; Strelow, Olaf
- Computer Aided Chemical Engineering, Vol. 21, p. 1863-1868
Method and Device for Detecting the Presence of a Single Target Nucleic Acid in a Sample
patent-application, July 2008
- Brown, James F.; Silver, Jonathan E.
- US Patent Application 11/837656; 20080171327
On-Chip, Real-Time, Single-Copy Polymerase Chain Reaction in Picoliter Droplets
journal, November 2007
- Beer, N. Reginald; Hindson, Benjamin J.; Wheeler, Elizabeth K.
- Analytical Chemistry, Vol. 79, Issue 22, p. 8471-8475
Bend test for a wire harness and device for such a test
patent, December 2002
- Kawakita, Yuki; Kawabe, Hitoshi; Inoue, Takuya
- US Patent Document 6,494,104
Nucleic acid amplification using microfluidic devices
patent-application, October 2005
- Enzelberger, Markus M.; Hansen, Carl L.; Liu, Jian
- US Patent Application 11/133805; 20050221373
Thermocapillary valve for droplet production and sorting
journal, April 2007
- Baroud, Charles N.; Delville, Jean-Pierre; Gallaire, Francois
- Physical Review E, Vol. 75, Issue 4, Article No. 046302
Integrated nucleic acid diagnostic device
patent, January 1999
- Lipshutz, Robert J.; Rava, Richard P.; Anderson, Rolfe
- US Patent Document 5,856,174
Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations
journal, July 2003
- Dressman, D.; Yan, H.; Traverso, G.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 15, p. 8817-8822
Method and systems for laser treatment of presbyopia using offset imaging
patent, December 2003
- Odrich, Marc; Greenberg, Kenneth; Legerton, Jerome A.
- US Patent Document 6,663,619
Coupling to microstructures for a laboratory microchip
patent, August 2003
- Zimmermann, Hans-Peter; Ple, Gerhard
- US Patent Document 6,602,472
High throughput screening assay systems in microscale fluidic devices
patent, August 2006
- Parce, J. Wallace; Kopf-Sill, Anne R.; Bousse, Luc
- US Patent Document 7,091,048
Controlled fluid transport microfabricated polymeric substrates
patent, December 2000
- Parce, John Wallace; Knapp, Michael R.; Chow, Calvin Y. H.
- US Patent Document 6,156,181
Klenow fragment and DNA polymerase α-primase fromserva calf thymus in water-in-oil microemulsions
journal, May 1998
- Anarbaev, Rashid O.; Elepov, Ilja B.; Lavrik, Olga I.
- Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 1384, Issue 2
Methods of detecting amplified product
patent, November 2011
- Brown, James F.; Silver, Jonathan E.
- US Patent Document 8,067,159
Method for detecting the presence of a single target nucleic acid in a sample
patent-application, September 2004
- Brown, James F.
- US Patent Application 10/798857; 20040171055
Method and Device for Detecting the Presence of a Single Target Nucleic Acid in a Sample
patent-application, July 2008
- Brown, James F.; Silver, Jonatan E.
- US Patent Application 11/837559; 20080160525
System for autonomous monitoring of bioagents
patent-application, March 2006
- Dzwnitis, John M.; Brown, STeve B.; Colston, JR., Billy W.
- US Patent Application 11/000491; 20060057599
Late-PCR
patent, April 2007
- Wangh, Lawrence J.; Pierce, Kenneth; Hartshorn, Cristina
- US Patent Document 7,198,897
On-Chip Single-Copy Real-Time Reverse-Transcription PCR in Isolated Picoliter Droplets
journal, March 2008
- Beer, N. Reginald; Wheeler, Elizabeth K.; Lee-Houghton, Lorenna
- Analytical Chemistry, Vol. 80, Issue 6, p. 1854-1858
Methods and systems for processing microfluidic samples of particle containing fluids
patent-application, October 2002
- Parunak, Gene; Handique, Kalyan; Wu, Betty
- US Patent Application 10/014520; 20020141903
Methods of amplifying and sequencing nucleic acids
patent, January 2008
- Leamon, John H.; Lohman, Kenton L.; Rothberg, Jonathan M.
- US Patent Document 7,323,305
Device for displacement of small liquid volumes along a micro-catenary line by electrostatic forces
patent, May 2006
- Fouillet, Yves; Charles, Raymond; Constantin, Olivier
- US Patent Document 7,052,244
Microfluidic systems incorporating varied channel dimensions
patent, December 1998
- Kopf-Sill, Anne R.; Parce, John Wallace
- US Patent Document 5,842,787
Microfluidic devices for the controlled manipulation of small volumes
patent, July 2007
- Ramsey, J. Michael; Jacobson, Stephen C.
- US Patent Document 7,238,268
Catheter guided by optical coherence domain reflectometry
patent, May 2002
- Everett, Matthew J.; Colston, JR., Billy W.; Da Silva, Luiz B.
- US Patent Document 6,384,915
Using Three-Phase Flow of Immiscible Liquids To Prevent Coalescence of Droplets in Microfluidic Channels: Criteria To Identify the Third Liquid and Validation with Protein Crystallization
journal, February 2007
- Chen, Delai L.; Li, Liang; Reyes, Sebastian
- Langmuir, Vol. 23, Issue 4
Chemical amplification based on fluid partitioning
patent, May 2006
- Anderson, Brian L.; Colston, JR., Billy W.; Elkin, Chris
- US Patent Document 7,041,481
Apparatus for repeated automatic execution of a thermal cycle for treatment of samples
patent, January 1993
- Larzul, M. Daniel
- US Patent Document 5,176,203
Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
patent, May 2002
- Brown, James F.; Silver, Jonathan E.; Kalinina, Olga
- US Patent Document 6,391,559
Assay apparatus and method using microfluidic arrays
patent, March 2010
- Linton, John; Yoder, Karl; Hess, Robert
- US Patent Document 7,682,565
Nucleic acid amplification using microfluidic devices
patent, November 2010
- Enzelberger, Markus; Hansen, Carl L.; Liu, Jian
- US Patent Document 7,833,708
Device Having Regions of Differing Affinities to Fluid, Methods of Making Such a Devices, and Methods of Using Such Devices.
patent-application, July 2008
- Brown, James F.; Silver, Jonathan E.
- US Patent Application 11/837600; 20080169184
Fluorescence monitoring device with textured optical tube and method for reducing background fluorescence
patent, October 1999
- Ragusa, Robert P.; Woudenberg, Timothy; Marmaro, Jeffrey M.
- US Patent Document 5,972,716
Microparticle Concentration and Separation by Traveling-Wave Dielectrophoresis (twDEP) for Digital Microfluidics
journal, December 2007
- Zhao, Yuejun; Yi, Ui-Chong; Cho, Sung Kwon
- Journal of Microelectromechanical Systems, Vol. 16, Issue 6, p. 1472-1481
Electric Control of Droplets in Microfluidic Devices
journal, April 2006
- Link, Darren R.; Grasland-Mongrain, Erwan; Duri, Agnes
- Angewandte Chemie International Edition, Vol. 45, Issue 16, p. 2556-2560
Device for multiple analyte detection
patent, October 2000
- Woudenberg, Timothy; Albin, Michael; Kowallis, Reid B.
- US Patent Document 6,126,899
Bead emulsion nucleic acid amplification
patent-application, April 2005
- Berka, Jan; Chen, Yi-Ju; Leamon, John H.
- US Patent Application 10/767899; 20050079510
Droplet-based microfluidic oligonucleotide synthesis engine
patent-application, September 2003
- Gascoyne, Peter; Vykoukal, Jody V.; Schwartz, Jon
- US Patent Application 10/336609; 20030170698
Microfluidic devices for controlled viscous shearing and formation of amphiphilic vesicles
patent-application, February 2005
- Lee, Abraham Phillip; Tan, Yung-Chieh
- US Patent Application 10/777470; 20050032240
Digital quantification of mutant DNA in cancer patients
journal, January 2007
- Diehl, Frank; Diaz, Luis A.
- Current Opinion in Oncology, Vol. 19, Issue 1, p. 36-42
Microfluidic device and methods of using same
patent, October 2006
- Unger, Marc A.; Manger, Ian D.; Lucero, Michael
- US Patent Document 7,118,910
Continuous manufacturing method for microspheres and apparatus
patent, January 2001
- Nakajima, Mitsutoshi; Kikuchi, Yuji; Sano, Yoh
- US Patent Document 6,177,479
Methods and systems for processing microfluidic samples of particle containing fluids
patent, September 2007
- Parunak, Gene; Handique, Kalyan; Wu, Betty
- US Patent Document 7,270,786
Methods for analysis and sorting of polynucleotides
patent, February 2002
- Quake, Stephen R.; Volkmuth, Wayne
- US Patent Document 6,344,325
Coupling Microdroplet Microreactors with Mass Spectrometry: Reading the Contents of Single Droplets Online
journal, May 2009
- Fidalgo, Luis M.; Whyte, Graeme; Ruotolo, Brandon T.
- Angewandte Chemie International Edition, Vol. 48, Issue 20, p. 3665-3668
Principle and applications of digital PCR
journal, January 2004
- Pohl, Gudrun; Shih, Ie-Ming
- Expert Review of Molecular Diagnostics, Vol. 4, Issue 1
System for real time detection of nucleic acid amplification products
patent, July 1999
- Woudenberg, Timothy; Bodner, Kevin S.; Connell, Charles R.
- US Patent Document 5,928,907
Method for preparing single-stranded DNA libraries
patent-application, September 2004
- Costa, Gina L.; Leamon, John H.; Rothberg, Jonathan M.
- US Patent Application 10/767894; 20040185484
Microfabricated cell sorter for chemical and biological materials
patent, April 2003
- Spence, Charles F.; Fu, Anne Y.; Quake, Stephen R.
- US Patent Document 6,540,895
Microfabricated crossflow devices and methods
patent, November 2007
- Quake, Stephen R.; Thorsen, Todd A.
- US Patent Document 7,294,503
Droplet fusion by alternating current (AC) field electrocoalescence in microchannels
journal, October 2005
- Chabert, Max; Dorfman, Kevin D.; Viovy, Jean-Louis
- ELECTROPHORESIS, Vol. 26, Issue 19, p. 3706-3715
Nucleic acid amplification and detection using microfluidic diffusion based structures
patent-application, November 2001
- Schulte, Thomas H.; Weigl, Bernhard H.
- US Patent Application 09/865093; 20010046701
Nucleic acid amplification with continuous flow emulsion
patent-application, October 2005
- Nobile, John R.; Lee, William L.; Leamon, John H.
- US Patent Application 11/045678; 20050227264
High Throughput Device for Performing Continuous-Flow Reactions
patent-application, June 2008
- Hahn, Jong Hoon; Park, Nokyoung; Lim, Kwanseop
- US Patent Application 10/588159; 20080145923
Method of Loading Sample Into a Microfluidic Device
patent-application, June 2008
- Brown, James F.; Silver, Jonathan E.
- US Patent Application 11/837608; 20080138815
Optical sorting method
patent-application, December 2009
- Griffiths, Andrew; Tawfik, DAn; Sepp, Armin
- US Patent Application 12/317123; 20090325236
Droplet-Based Microfluidic Platforms for the Encapsulation and Screening of Mammalian Cells and Multicellular Organisms
journal, May 2008
- Clausell-Tormos, Jenifer; Lieber, Diana; Baret, Jean-Christophe
- Chemistry & Biology, Vol. 15, Issue 5, p. 427-437
Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
patent-application, November 2002
- Brown, James F.
- US Patent Application 10/131854; 20020164820
Digital PCR
journal, August 1999
- Vogelstein, B.; Kinzler, K. W.
- Proceedings of the National Academy of Sciences, Vol. 96, Issue 16
Digital PCR for the molecular detection of fetal chromosomal aneuploidy
journal, July 2007
- Lo, Y. M. D.; Lun, F. M. F.; Chan, K. C. A.
- Proceedings of the National Academy of Sciences, Vol. 104, Issue 32, p. 13116-13121
Cell fixative composition and method of staining cells without destroying the cell surface
patent, June 1995
- Connelly, Mark Carle; Chakraborty, Utpal R.; Brooks, Jr., Houston G.
- US Patent Document 5,422,277
Bead emulsion nucleic acid amplification
patent, November 2010
- Berka, Jan; Chen, Yi-Ju; Leamon, John H.
- US Patent Document 7,842,457
Methods and systems for fluid control in microfluidic devices
patent, June 2003
- Parunak, Gene
- US Patent Document 6,575,188
Method for chemical amplification based on fluid partitioning in an immiscible liquid
patent, June 2015
- Anderson, Brian L.; Colston, Bill W.; Elkin, Christopher J.
- US Patent Document RE45,539
Chemical Amplification: Continuous-Flow PCR on a Chip
journal, May 1998
- Kopp, Martin U.; de Mello, Andrew J.; Manz, Andreas
- Science, Vol. 280, Issue 5366, p. 1046-1048
Overview: methods and applications for droplet compartmentalization of biology
journal, July 2006
- Leamon, John H.; Link, Darren R.; Egholm, Michael
- Nature Methods, Vol. 3, Issue 7
Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends
journal, June 2007
- Zhang, Chunsun; Xing, Da
- Nucleic Acids Research, Vol. 35, Issue 13, p. 4223-4237
Microfluidic Assembly with Reagent
patent-application, July 2008
- Brown, James F.; Silver, Jonathan E.
- US Patent Application 11/837561; 20080171380
Droplet-Based Microfluidics for Emulsion and Solvent Evaporation Synthesis of Monodisperse Mesoporous Silica Microspheres
journal, February 2008
- Carroll, Nick J.; Rathod, Shailendra B.; Derbins, Erin
- Langmuir, Vol. 24, Issue 3, p. 658-661
Nucleic acid amplification with continuous flow emulsion
patent, April 2011
- Nobile, John R.; Lee, William L.; Leamon, John H.
- US Patent Document 7,927,797
Emulsion compositions
patent, November 2009
- Holliger, Phillip; Ghadessy, Farid
- US Patent Document 7,622,280
High-Throughput Single Copy DNA Amplification and Cell Analysis in Engineered Nanoliter Droplets
journal, May 2008
- Kumaresan, Palani; Yang, Chaoyong James; Cronier, Samantha A.
- Analytical Chemistry, Vol. 80, Issue 10, p. 3522-3529
Pre-disposed assay components in microfluidic devices and methods
patent, November 2002
- Parce, J. Wallace; Kopf-Sill, Anne R.; Bousse, Luc
- US Patent Document 6,479,299
Method and Device for Detecting Presence of a Single Target Nucleic Acid in a Sample
patent-application, July 2008
- Brown, James F.; Silver, Jonathan E.
- US Patent Application 11/837564; 20080171382
Microfluidic devices and methods for performing temperature mediated reactions
patent, December 2003
- Stern, Seth
- US Patent Document 6,670,153
Inline thermo-cycler
patent, April 1998
- Hayes, Donald J.; Wallace, David B.; Frederickson, Christopher J.
- US Patent Document 5,736,314
Genome sequencing in microfabricated high-density picolitre reactors
journal, July 2005
- Margulies, Marcel; Egholm, Michael; Altman, William E.
- Nature, Vol. 437, Issue 7057, p. 376-380
Megapixel digital PCR
journal, July 2011
- Heyries, Kevin A.; Tropini, Carolina; VanInsberghe, Michael
- Nature Methods, Vol. 8, Issue 8
Droplet Formation Utilizing Controllable Moving-Wall Structures for Double-Emulsion Applications
journal, June 2008
- Lin, Yen-Heng; Lee, Chun-Hong; Lee, Gwo-Bin
- Journal of Microelectromechanical Systems, Vol. 17, Issue 3, p. 573-581
Amplification of complex gene libraries by emulsion PCR
journal, July 2006
- Williams, Richard; Peisajovich, Sergio G.; Miller, Oliver J.
- Nature Methods, Vol. 3, Issue 7, p. 545-550
Crystal growth devices and systems, and methods for using same
patent, October 2007
- Nassef, Hany Ramez; Facer, Geoffrey; Barco, Joseph W.
- US Patent Document 7,279,146
High-throughput screening assay systems in microscale fluidic devices
patent, August 2002
- Parce, John Wallace; Kopf-Sill, Anne R.; Bousse, Luc
- US Patent Document 6,429,025
Early detection of contagious diseases
patent-application, October 2003
- Colston, JR., Billy W.; Milanovich, Fred P.; Estacio, Pedro
- US Patent Application 10/375273; 20030204130
Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
patent-application, November 2006
- Bohm, Sebastian; Gilbert, John
- US Patent Application 11/486186; 20060263264
Analysis system
patent, August 1981
- Cormier, Alan D.; Webster, Milo E.; Czaban, John D.
- US Patent Document 4,283,262
Methods for isolating mutant microoganisms using microcapsules coated with indicator material
patent, January 1989
- Perlman, Daniel
- US Patent Document 4,801,529
Automated nucleic acid assay system
patent-application, February 2003
- Colston, JR., Billy W.; Brown, Steve B.; Nasarabadi, Shanavaz L.
- US Patent Application 10/189319; 20030032172
Assay apparatus and method using microfluidic arrays
patent-application, October 2004
- Linton, John; Yoder, Karl; Hess, Robert
- US Patent Application 10/744580; 20040208792
Apparatus for Dispensing Liquid Sample
patent, April 1971
- Davis, Robert; Shlisky, Theodore; Silverman, John
- US Patent Document 3,575,220
Double ended sequencing
patent, July 2007
- Chen, Yi-Ju; Leamon, John H.; Lohman, Kenton L.
- US Patent Document 7,244,567
Development of A Microchamber Array for Picoliter PCR
journal, March 2001
- Nagai, Hidenori; Murakami, Yuji; Morita, Yasutaka
- Analytical Chemistry, Vol. 73, Issue 5, p. 1043-1047
Miniaturized assembly and method of filling assembly
patent, December 2008
- Brown, James F.
- US Patent Document 7,459,315
Method and Device for Detecting the Presence of a Single Target Nucleic Acid in a Sample
patent-application, July 2008
- Brown, James F.; Silver, Jonathan E.
- US Patent Application 11/837651; 20080171326
Microchannel apparatus and method of producing emulsions making use thereof
patent, August 2001
- Nakajima, Mitsutoshi; Nabetani, Hiroshi; Kikuchi, Yuji
- US Patent Document 6,281,254
High throughput screening for novel enzymes
patent, January 2001
- Short, Jay M.; Keller, Martin
- US Patent Document 6,174,673
Binding phenol oxidizing enzyme-peptide complexes
patent-application, February 2005
- Aehle, Wolfgang; Baldwin, Toby L.; Van Gastel, Franciscus J. C>
- US Patent Application 10/912512; 20050042684
Microfabricated Crossflow Devices and Methods
patent-application, February 2009
- Quake, Stephen R.; Thorsen, Todd
- US Patent Application 11/868942; 20090035838
Methods and systems for control of microfluidic devices
patent, March 2006
- Handique, Kalyan; Ganesan, Karthik; Brahmasandra, Sundaresh N.
- US Patent Document 7,010,391
Microfluidic Devices for the Controlled Manipulation of Small Volumes
patent-application, October 2007
- Ramsey, Michael J.; Jacobson, Stephen C.
- US Patent Application 11/696785; 20070227890
Single-molecule PCR using water-in-oil emulsion
journal, April 2003
- Nakano, Michihiko; Komatsu, Jun; Matsuura, Shun-ichi
- Journal of Biotechnology, Vol. 102, Issue 2, p. 117-124
Fabrication of microfluidic circuits by printing techniques
patent, January 2003
- Kennedy, Colin B.
- US Patent Document 6,509,085
Optical sorting method
patent-application, August 2002
- Griffiths, Andrew
- US Patent Application 09/896915; 20020119459
Multiplexed microfluidic devices, systems, and methods
patent, December 2002
- Kennedy, Colin B.
- US Patent Document 6,488,895
Magnetic tumble stirring method, devices and machines for mixing in vessels
patent, January 2001
- Cleveland, Patrick H.; Markle, John R.
- US Patent Document 6,176,609
Method For Monitoring Disease Progression or Recurrence
patent-application, July 2008
- Shuber, Anthony P.
- US Patent Application 11/666561; 20080161420
Look before You Leap: Active Surveillance for Multidrug-Resistant Organisms
journal, April 2007
- Weinstein, Robert A.; Diekema, Daniel J.; Edmond, Michael B.
- Clinical Infectious Diseases, Vol. 44, Issue 8, p. 1101-1107
Process for producing emulsion and microcapsules and apparatus therefor
patent, September 2007
- Higuchi, Toshiro; Torii, Toru; Nishisako, Takashi
- US Patent Document 7,268,167
Mathematics of quantitative kinetic PCR and the application of standard curves
journal, August 2003
- Rutledge, R. G.; Cote, C.
- Nucleic Acids Research, Vol. 31, Issue 16, p. 93e-93
Apparatus and method for performing electrodynamic focusing on a microchip
patent, January 1999
- Ramsey, John Michael; Jacobson, Stephen C.
- US Patent Document 5,858,187
Nucleic acid amplification utilizing microfluidic devices
patent, November 2005
- Enzelberger, Markus; Liu, Jian; Quake, Stephen R.
- US Patent Document 6,960,437
Method for detecting the presence of a single target nucleic acid in a sample
patent, October 2012
- Brown, James F.; Silver, Jonathan E.
- US Patent Document 8,278,071
Optical fiber head for providing lateral viewing
patent-application, February 2002
- Everett, Matthew J.; Colston, Billy W.; James, Dale L.
- US Patent Application 09/883513; 20020021866
Digital amplification
patent, August 2002
- Vogelstein, Bert; Kinzler, Kenneth W.
- US Patent Document 6,440,706
Device for parallel and synchronous injection for sequential injection of different reagents
patent, August 2006
- Fouillet, Yves; Sarrut, Nicolas; Gruss, Antoine
- US Patent Document 7,094,379
Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions
journal, September 1993
- Higuchi, Russell; Fockler, Carita; Dollinger, Gavin
- Nature Biotechnology, Vol. 11, Issue 9
Thermal cycler for microfluidic array assays
patent-application, May 2006
- Yoder, Karl; Brenan, Colin; Linton, John
- US Patent Application 11/227425; 20060094108
Hydrophobic coating compositions, articles coated with said compositions, and processes for manufacturing same
patent, September 2007
- Brown, James F.
- US Patent Document 7,268,179
Chemical amplification based on fluid partitioning
patent-application, September 2004
- Anderson, Brian L.; Colston, JR., Billy W.; Elkin, Chris
- US Patent Application 10/389130; 20040180346
Behavioral Modeling and Performance Evaluation of Microelectrofluidics-Based PCR Systems Using SystemC
journal, June 2004
- Zhang, Tianhao; Chakrabarty, K.; Fair, R. B.
- IEEE Transactions on Computer-Aided Design of Integrated Circuits and Systems, Vol. 23, Issue 6, p. 843-858
Miniaturized flow-through PCR with different template types in a silicon chip thermocycler
journal, January 2001
- Schneegaß, Ivonne; Bräutigam, Reiner; Köhler, Johann Michael
- Lab Chip, Vol. 1, Issue 1
Variable flexure-based fluid filter
patent-application, April 2004
- Brown, Steve B.; Colston, JR., Billy W.; Marshall, Graham
- US Patent Application 10/648626; 20040074849
Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
patent, November 2000
- Brown, James F.; Silver, Jonathan E.; Kalinina, Olga
- US Patent Document 6,143,496
Continuous flow separations in microfluidic devices
journal, January 2007
- Pamme, Nicole
- Lab on a Chip, Vol. 7, Issue 12, p. 1644-1659
Portable pathogen detection system
patent, June 2005
- Colston, JR., Billy W.; Everett, Matthew J.; Milanovich, Fred P.
- US Patent Document 6,905,885
Controlling Nonspecific Protein Adsorption in a Plug-Based Microfluidic System by Controlling Interfacial Chemistry Using Fluorous-Phase Surfactants
journal, February 2005
- Roach, L. Spencer; Song, Helen; Ismagilov, Rustem F.
- Analytical Chemistry, Vol. 77, Issue 3
Electrostatic actuators for microfluidics and methods for using same
patent, August 2004
- Shenderov, Alexander
- US Patent Document 6,773,566