Synthetic methylotrophy to liquid fuels and chemicals
Abstract
A non-naturally occurring microbe capable of growing in a medium comprising methanol is provided. The methanol contributes to a significant percentage (e.g., at least 40%) of the carbon source for the non-naturally occurring microbe, which expresses heterologous methanol dehydrogenase (MDH) and heterologous ribulose monophosphate (RuMP) pathway enzymes. Methods for producing liquid fuels and chemicals by the non-naturally occurring microbe and methods for preparing the non-naturally occurring microbe are also provided.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Delaware, Newark, DE (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1477456
- Patent Number(s):
- 10059920
- Application Number:
- 15/112,364
- Assignee:
- University of Delaware (Newark, DE)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- AR0000432
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2015 Jan 09
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Papoutsakis, Eleftherios T., Nicolaou, Sergios, Fast, Alan, Falara, Vasiliki, Bennett, Robert Kyle, Whitaker, William Brian, Sandoval, Nicholas Richard, Gonzalez, Jacqueline, and Antoniewicz, Maciek. Synthetic methylotrophy to liquid fuels and chemicals. United States: N. p., 2018.
Web.
Papoutsakis, Eleftherios T., Nicolaou, Sergios, Fast, Alan, Falara, Vasiliki, Bennett, Robert Kyle, Whitaker, William Brian, Sandoval, Nicholas Richard, Gonzalez, Jacqueline, & Antoniewicz, Maciek. Synthetic methylotrophy to liquid fuels and chemicals. United States.
Papoutsakis, Eleftherios T., Nicolaou, Sergios, Fast, Alan, Falara, Vasiliki, Bennett, Robert Kyle, Whitaker, William Brian, Sandoval, Nicholas Richard, Gonzalez, Jacqueline, and Antoniewicz, Maciek. Tue .
"Synthetic methylotrophy to liquid fuels and chemicals". United States. https://www.osti.gov/servlets/purl/1477456.
@article{osti_1477456,
title = {Synthetic methylotrophy to liquid fuels and chemicals},
author = {Papoutsakis, Eleftherios T. and Nicolaou, Sergios and Fast, Alan and Falara, Vasiliki and Bennett, Robert Kyle and Whitaker, William Brian and Sandoval, Nicholas Richard and Gonzalez, Jacqueline and Antoniewicz, Maciek},
abstractNote = {A non-naturally occurring microbe capable of growing in a medium comprising methanol is provided. The methanol contributes to a significant percentage (e.g., at least 40%) of the carbon source for the non-naturally occurring microbe, which expresses heterologous methanol dehydrogenase (MDH) and heterologous ribulose monophosphate (RuMP) pathway enzymes. Methods for producing liquid fuels and chemicals by the non-naturally occurring microbe and methods for preparing the non-naturally occurring microbe are also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {8}
}
Works referenced in this record:
Metabolic Engineering of Corynebacterium glutamicum for Methanol Metabolism
journal, January 2015
- Witthoff, Sabrina; Schmitz, Katja; Niedenführ, Sebastian
- Applied and Environmental Microbiology, Vol. 81, Issue 6, p. 2215-2225
Growth dynamics of a methylotroph (Methylomonas L3) in continuous cultures. I. Fast transients induced by methanol pulses and methanol accumulation
journal, January 1987
- Chu, I-Ming; Papoutsakis, Eleftherios Terry
- Biotechnology and Bioengineering, Vol. 29, Issue 1, p. 55-64
Sort-Seq Approach to Engineering a Formaldehyde-Inducible Promoter for Dynamically Regulated Escherichia coli Growth on Methanol
journal, May 2017
- Rohlhill, Julia; Sandoval, Nicholas R.; Papoutsakis, Eleftherios T.
- ACS Synthetic Biology, Vol. 6, Issue 8, p. 1584-1595
Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase
journal, May 2007
- Orita, Izumi; Sakamoto, Naoki; Kato, Nobuo
- Applied Microbiology and Biotechnology, Vol. 76, Issue 2, p. 439-445
Plasmid-Dependent Methylotrophy in Thermotolerant Bacillus methanolicus
journal, February 2004
- Brautaset, T.; Jakobsen, O. M.; Flickinger, M. C.
- Journal of Bacteriology, Vol. 186, Issue 5, p. 1229-1238
Formaldehyde Fixation Contributes to Detoxification for Growth of a Nonmethylotroph, Burkholderia cepacia TM1, on Vanillic Acid
journal, October 2003
- Mitsui, R.; Kusano, Y.; Yurimoto, H.
- Applied and Environmental Microbiology, Vol. 69, Issue 10, p. 6128-6132
The colorimetric estimation of formaldehyde by means of the Hantzsch reaction
journal, October 1953
- Nash, T.
- Biochemical Journal, Vol. 55, Issue 3
Engineering Escherichia coli for methanol conversion
journal, March 2015
- Müller, Jonas E. N.; Meyer, Fabian; Litsanov, Boris
- Metabolic Engineering, Vol. 28, p. 190-201
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
journal, May 2000
- Datsenko, K. A.; Wanner, B. L.
- Proceedings of the National Academy of Sciences, Vol. 97, Issue 12, p. 6640-6645
Upregulated Transcription of Plasmid and Chromosomal Ribulose Monophosphate Pathway Genes Is Critical for Methanol Assimilation Rate and Methanol Tolerance in the Methylotrophic Bacterium Bacillus methanolicus
journal, April 2006
- Jakobsen, O. M.; Benichou, A.; Flickinger, M. C.
- Journal of Bacteriology, Vol. 188, Issue 8, p. 3063-3072
Production of bio-oil and biochar from soapstock via microwave-assisted co-catalytic fast pyrolysis
journal, February 2017
- Dai, Leilei; Fan, Liangliang; Liu, Yuhuan
- Bioresource Technology, Vol. 225, p. 1-8