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Title: Synthetic methylotrophy to liquid fuels and chemicals

Abstract

A non-naturally occurring microbe capable of growing in a medium comprising methanol is provided. The methanol contributes to a significant percentage (e.g., at least 40%) of the carbon source for the non-naturally occurring microbe, which expresses heterologous methanol dehydrogenase (MDH) and heterologous ribulose monophosphate (RuMP) pathway enzymes. Methods for producing liquid fuels and chemicals by the non-naturally occurring microbe and methods for preparing the non-naturally occurring microbe are also provided.

Inventors:
; ; ; ; ; ; ; ;
Issue Date:
Research Org.:
Univ. of Delaware, Newark, DE (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1477456
Patent Number(s):
10059920
Application Number:
15/112,364
Assignee:
University of Delaware (Newark, DE)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
AR0000432
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015 Jan 09
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Papoutsakis, Eleftherios T., Nicolaou, Sergios, Fast, Alan, Falara, Vasiliki, Bennett, Robert Kyle, Whitaker, William Brian, Sandoval, Nicholas Richard, Gonzalez, Jacqueline, and Antoniewicz, Maciek. Synthetic methylotrophy to liquid fuels and chemicals. United States: N. p., 2018. Web.
Papoutsakis, Eleftherios T., Nicolaou, Sergios, Fast, Alan, Falara, Vasiliki, Bennett, Robert Kyle, Whitaker, William Brian, Sandoval, Nicholas Richard, Gonzalez, Jacqueline, & Antoniewicz, Maciek. Synthetic methylotrophy to liquid fuels and chemicals. United States.
Papoutsakis, Eleftherios T., Nicolaou, Sergios, Fast, Alan, Falara, Vasiliki, Bennett, Robert Kyle, Whitaker, William Brian, Sandoval, Nicholas Richard, Gonzalez, Jacqueline, and Antoniewicz, Maciek. Tue . "Synthetic methylotrophy to liquid fuels and chemicals". United States. https://www.osti.gov/servlets/purl/1477456.
@article{osti_1477456,
title = {Synthetic methylotrophy to liquid fuels and chemicals},
author = {Papoutsakis, Eleftherios T. and Nicolaou, Sergios and Fast, Alan and Falara, Vasiliki and Bennett, Robert Kyle and Whitaker, William Brian and Sandoval, Nicholas Richard and Gonzalez, Jacqueline and Antoniewicz, Maciek},
abstractNote = {A non-naturally occurring microbe capable of growing in a medium comprising methanol is provided. The methanol contributes to a significant percentage (e.g., at least 40%) of the carbon source for the non-naturally occurring microbe, which expresses heterologous methanol dehydrogenase (MDH) and heterologous ribulose monophosphate (RuMP) pathway enzymes. Methods for producing liquid fuels and chemicals by the non-naturally occurring microbe and methods for preparing the non-naturally occurring microbe are also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {8}
}

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Works referenced in this record:

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Growth dynamics of a methylotroph (Methylomonas L3) in continuous cultures. I. Fast transients induced by methanol pulses and methanol accumulation
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