skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Nucleic acids useful for integrating into and gene expression in hyperthermophilic acidophilic Archaea

Abstract

The present invention provides for a novel recombinant or isolated nucleic acid useful for integrating or being maintained in an Archaea or acidophilic hyperthermophilic eubacteria. The nucleic acid encodes a nucleotide sequence that is capable of stably integrating into the chromosome of a host cell, or being maintained as an extrachromosomal element in a host cell, that is an Archea, and a nucleotide sequence of interest. The present invention also provides for an Archaea host cell comprising the nucleic acid stably integrated into the chromosome or maintained episomally in the host cell, and a method of expressing the nucleotide sequence of interest in the host cell and/or directing glycosylation, multimerization, and/or membrane association or integration.

Inventors:
;
Issue Date:
Research Org.:
Lawrence Berkeley National Laboratory, E-Scholarship Repository, Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1476878
Patent Number(s):
10066223
Application Number:
14/646,673
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 Nov 21
Country of Publication:
United States
Language:
English

Citation Formats

Yannone, Steven M., and Barnebey, Adam. Nucleic acids useful for integrating into and gene expression in hyperthermophilic acidophilic Archaea. United States: N. p., 2018. Web.
Yannone, Steven M., & Barnebey, Adam. Nucleic acids useful for integrating into and gene expression in hyperthermophilic acidophilic Archaea. United States.
Yannone, Steven M., and Barnebey, Adam. Tue . "Nucleic acids useful for integrating into and gene expression in hyperthermophilic acidophilic Archaea". United States. https://www.osti.gov/servlets/purl/1476878.
@article{osti_1476878,
title = {Nucleic acids useful for integrating into and gene expression in hyperthermophilic acidophilic Archaea},
author = {Yannone, Steven M. and Barnebey, Adam},
abstractNote = {The present invention provides for a novel recombinant or isolated nucleic acid useful for integrating or being maintained in an Archaea or acidophilic hyperthermophilic eubacteria. The nucleic acid encodes a nucleotide sequence that is capable of stably integrating into the chromosome of a host cell, or being maintained as an extrachromosomal element in a host cell, that is an Archea, and a nucleotide sequence of interest. The present invention also provides for an Archaea host cell comprising the nucleic acid stably integrated into the chromosome or maintained episomally in the host cell, and a method of expressing the nucleotide sequence of interest in the host cell and/or directing glycosylation, multimerization, and/or membrane association or integration.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {9}
}

Patent:

Save / Share:

Works referenced in this record:

Archaeal flagellar ATPase motor shows ATP-dependent hexameric assembly and activity stimulation by specific lipid binding
journal, July 2011


A reporter gene system for the hyperthermophilic archaeon Sulfolobus solfataricus based on a selectable and integrative shuttle vector
journal, June 2003


Improvement of reporter activity by IRES-mediated polycistronic reporter system
journal, January 2008


Structural Organization of Essential Iron-Sulfur Clusters in the Evolutionarily Highly Conserved ATP-binding Cassette Protein ABCE1
journal, March 2007


Thermostable beta-galactosidase from the archaebacterium Sulfolobus solfataricus Purification and properties
journal, January 1990


Production of Recombinant and Tagged Proteins in the Hyperthermophilic Archaeon Sulfolobus solfataricus
journal, January 2006


An upstream activation element exerting differential transcriptional activation on an archaeal promoter
journal, October 2009