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Title: Compositions and methods for making selenocysteine containing polypeptides

Non-naturally occurring tRNA.sup.Sec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNASec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNASec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNASec.
Inventors:
; ;
Issue Date:
OSTI Identifier:
1466033
Assignee:
Yale University (New Haven, CT) CHO
Patent Number(s):
10,023,893
Application Number:
15/274,541
Contract Number:
FG02-98ER20311
Resource Relation:
Patent File Date: 2016 Sep 23
Research Org:
Yale Univ., New Haven, CT (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English

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