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Title: Compositions and methods for making selenocysteine containing polypeptides

Abstract

Non-naturally occurring tRNA.sup.Sec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNASec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNASec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNASec.

Inventors:
; ;
Issue Date:
Research Org.:
Yale Univ., New Haven, CT (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1466033
Patent Number(s):
10023893
Application Number:
15/274,541
Assignee:
Yale University (New Haven, CT)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
FG02-98ER20311
Resource Type:
Patent
Resource Relation:
Patent File Date: 2016 Sep 23
Country of Publication:
United States
Language:
English

Citation Formats

Soll, Dieter, Aldag, Caroline, and Hohn, Michael. Compositions and methods for making selenocysteine containing polypeptides. United States: N. p., 2018. Web.
Soll, Dieter, Aldag, Caroline, & Hohn, Michael. Compositions and methods for making selenocysteine containing polypeptides. United States.
Soll, Dieter, Aldag, Caroline, and Hohn, Michael. Tue . "Compositions and methods for making selenocysteine containing polypeptides". United States. https://www.osti.gov/servlets/purl/1466033.
@article{osti_1466033,
title = {Compositions and methods for making selenocysteine containing polypeptides},
author = {Soll, Dieter and Aldag, Caroline and Hohn, Michael},
abstractNote = {Non-naturally occurring tRNA.sup.Sec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNASec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNASec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNASec.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {7}
}

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