skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Compositions and methods for making selenocysteine containing polypeptides

Abstract

Non-naturally occurring tRNA.sup.Sec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNA Sec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNA Sec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNA Sec.

Inventors:
; ;
Issue Date:
Research Org.:
Yale Univ., New Haven, CT (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1466033
Patent Number(s):
10,023,893
Application Number:
15/274,541
Assignee:
Yale University (New Haven, CT)
DOE Contract Number:  
FG02-98ER20311
Resource Type:
Patent
Resource Relation:
Patent File Date: 2016 Sep 23
Country of Publication:
United States
Language:
English

Citation Formats

Soll, Dieter, Aldag, Caroline, and Hohn, Michael. Compositions and methods for making selenocysteine containing polypeptides. United States: N. p., 2018. Web.
Soll, Dieter, Aldag, Caroline, & Hohn, Michael. Compositions and methods for making selenocysteine containing polypeptides. United States.
Soll, Dieter, Aldag, Caroline, and Hohn, Michael. Tue . "Compositions and methods for making selenocysteine containing polypeptides". United States. https://www.osti.gov/servlets/purl/1466033.
@article{osti_1466033,
title = {Compositions and methods for making selenocysteine containing polypeptides},
author = {Soll, Dieter and Aldag, Caroline and Hohn, Michael},
abstractNote = {Non-naturally occurring tRNA.sup.Sec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNASec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNASec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNASec.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {7}
}

Patent:

Save / Share:

Works referenced in this record:

Rewiring Translation for Elongation Factor Tu-Dependent Selenocysteine Incorporation
journal, November 2012

  • Aldag, Caroline; Bröcker, Markus J.; Hohn, Michael J.
  • Angewandte Chemie International Edition, Vol. 52, Issue 5, p. 1441-1445
  • DOI: 10.1002/anie.201207567

Basic local alignment search tool
journal, October 1990

  • Altschul, Stephen F.; Gish, Warren; Miller, Webb
  • Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
  • DOI: 10.1016/S0022-2836(05)80360-2

Cys-tRNACys formation and cysteine biosynthesis in methanogenic archaea: two faces of the same problem?
journal, October 2004

  • Ambrogelly, A.; Kamtekar, S.; Sauerwald, A.
  • Cellular and Molecular Life Sciences, Vol. 61, Issue 19-20, p. 2437-2445
  • DOI: 10.1007/s00018-004-4194-9

Structurally Modified Firefly Luciferase. Effects of Amino Acid Substitution at Position 286
journal, November 1997

  • Arslan, Tuncer; Mamaev, Sergey V.; Mamaeva, Natalia V.
  • Journal of the American Chemical Society, Vol. 119, Issue 45, p. 10877-10887
  • DOI: 10.1021/ja971927a

Mutagenesis-based definitions and probes of residue burial in proteins
journal, October 2005

  • Bajaj, K.; Chakrabarti, P.; Varadarajan, R.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 45, p. 16221-16226
  • DOI: 10.1073/pnas.0505089102

Phosphoserine Aminotransferase, the Second Step-Catalyzing Enzyme for Serine Biosynthesis
journal, November 1999

  • Basurko, Marie-Jose; Marche, Michele; Darriet, Monique
  • IUBMB Life, Vol. 48, Issue 5, p. 525-529
  • DOI: 10.1080/713803557

Photoreactive Bicyclic Amino Acids as Substrates for Mutant Escherichia coli Phenylalanyl-tRNA Synthetases
journal, March 2004

  • Bentin, Thomas; Hamzavi, Ramin; Salomonsson, Johan
  • Journal of Biological Chemistry, Vol. 279, Issue 19, p. 19839-19845
  • DOI: 10.1074/jbc.M401278200

Positive-selection vectors using the F plasmid ccdB killer gene
journal, October 1994


The unusual methanogenic seryl‐tRNA synthetase recognizes tRNASer species from all three kingdoms of life
journal, January 2004


Direct charging of tRNACUA with pyrrolysine in vitro and in vivo
journal, August 2004

  • Blight, Sherry K.; Larue, Ross C.; Mahapatra, Anirban
  • Nature, Vol. 431, Issue 7006, p. 333-335
  • DOI: 10.1038/nature02895

A method for increasing the yield of properly folded recombinant fusion proteins: Single-chain immunotoxins from renaturation of bacterial inclusion bodies
journal, September 1992

  • Buchner, Johannes; Pastan, Ira; Brinkmann, Ulrich
  • Analytical Biochemistry, Vol. 205, Issue 2, p. 263-270
  • DOI: 10.1016/0003-2697(92)90433-8

A dual-specificity aminoacyl-tRNA synthetase in the deep-rooted eukaryote Giardia lamblia
journal, November 2000

  • Bunjun, S.; Stathopoulos, C.; Graham, D.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 24, p. 12997-13002
  • DOI: 10.1073/pnas.230444397

Purification and Physical Characterization of Tyrosyl Ribonucleic Acid Synthetases from Escherichia coli and Bacillus subtilis
journal, May 1966

  • Calendar, Richard; Berg, Paul
  • Biochemistry, Vol. 5, Issue 5, p. 1681-1690
  • DOI: 10.1021/bi00869a033

The Catalytic Properties of Tyrosyl Ribonucleic Acid Synthetases from Escherichia coli and Bacillus subtilis
journal, May 1966

  • Calendar, Richard; Berg, Paul
  • Biochemistry, Vol. 5, Issue 5, p. 1690-1695
  • DOI: 10.1021/bi00869a034

Large Multiple Organism Gene Finding by Collapsed Gibbs Sampling
journal, July 2005

  • Chatterji, Sourav; Pachter, Lior
  • Journal of Computational Biology, Vol. 12, Issue 6, p. 599-608
  • DOI: 10.1089/cmb.2005.12.599

The Affinity of Elongation Factor Tu for an Aminoacyl-tRNA Is Modulated by the Esterified Amino Acid
journal, May 2004

  • Dale, Taraka; Sanderson, Lee E.; Uhlenbeck, Olke C.
  • Biochemistry, Vol. 43, Issue 20, p. 6159-6166
  • DOI: 10.1021/bi036290o

Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production
journal, January 2005

  • Daly, Rachel; Hearn, Milton T. W.
  • Journal of Molecular Recognition, Vol. 18, Issue 2, p. 119-138
  • DOI: 10.1002/jmr.687

Phenylalanyl-tRNA synthetase from the archaeon Methanobacterium thermoautotrophicum is an (αβ)2 heterotetrameric protein
journal, November 1999


One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
journal, May 2000

  • Datsenko, K. A.; Wanner, B. L.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 12, p. 6640-6645
  • DOI: 10.1073/pnas.120163297

Dynamics of Recognition between tRNA and Elongation Factor Tu
journal, April 2008

  • Eargle, John; Black, Alexis A.; Sethi, Anurag
  • Journal of Molecular Biology, Vol. 377, Issue 5, p. 1382-1405
  • DOI: 10.1016/j.jmb.2008.01.073

[15] Biosynthetic method for introducing unnatural amino acids site-specifically into proteins
book, January 1991

  • Ellman, Jon; Mendel, David; Anthony-Cahill, Spencer
  • Molecular Design and Modeling: Concepts and Applications Part A: Proteins, Peptides, and Enzymes, p. 301-336
  • DOI: 10.1016/0076-6879(91)02017-4

The insect tracheal system: a conduit for the systemic spread of Autographa californica M nuclear polyhedrosis virus.
journal, April 1994

  • Engelhard, E. K.; Kam-Morgan, L. N.; Washburn, J. O.
  • Proceedings of the National Academy of Sciences, Vol. 91, Issue 8, p. 3224-3227
  • DOI: 10.1073/pnas.91.8.3224

An aminoacyl tRNA synthetase whose sequence fits into neither of the two known classes
journal, May 2001

  • Fàbrega, Carme; Farrow, Mark A.; Mukhopadhyay, Biswarup
  • Nature, Vol. 411, Issue 6833, p. 110-114
  • DOI: 10.1038/35075121

High-level secretion of correctly processed recombinant human interleukin-1β in Kluyveromyces lactis
journal, November 1991


A proteomic view of the Plasmodium falciparum life cycle
journal, October 2002

  • Florens, Laurence; Washburn, Michael P.; Raine, J. Dale
  • Nature, Vol. 419, Issue 6906, p. 520-526
  • DOI: 10.1038/nature01107

Expression of genes transferred into monocot and dicot plant cells by electroporation.
journal, September 1985

  • Fromm, M.; Taylor, L. P.; Walbot, V.
  • Proceedings of the National Academy of Sciences, Vol. 82, Issue 17, p. 5824-5828
  • DOI: 10.1073/pnas.82.17.5824

Global analysis of a “simple” proteome: Methanococcus jannaschii
journal, December 2002

  • Giometti, Carol S.; Reich, Claudia; Tollaksen, Sandra
  • Journal of Chromatography B, Vol. 782, Issue 1-2, p. 227-243
  • DOI: 10.1016/S1570-0232(02)00568-8

Formation of de novo centromeres and construction of first-generation human artificial microchromosomes
journal, April 1997

  • Harrington, John J.; Bokkelen, Gil Van; Mays, Robert W.
  • Nature Genetics, Vol. 15, Issue 4, p. 345-355
  • DOI: 10.1038/ng0497-345

Emergence of the universal genetic code imprinted in an RNA record
journal, November 2006

  • Hohn, M. J.; Park, H.-S.; O'Donoghue, P.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 48, p. 18095-18100
  • DOI: 10.1073/pnas.0608762103

Isolation and identification of yeast messenger ribonucleic acids coding for enolase, glyceraldehyde-3-phosphate dehydrogenase, and phosphoglycerate kinase
journal, November 1978

  • Holland, Michael J.; Holland, Janice P.
  • Biochemistry, Vol. 17, Issue 23, p. 4900-4907
  • DOI: 10.1021/bi00616a007

The Adaptor hypothesis revisited
journal, July 2000

  • Ibba, Michael; Becker, Hubert D.; Stathopoulos, Constantinos
  • Trends in Biochemical Sciences, Vol. 25, Issue 7, p. 311-316
  • DOI: 10.1016/S0968-0004(00)01600-5

Drag&Drop cloning in yeast
journal, January 2005


Toward understanding phosphoseryl-tRNACys formation: The crystal structure of Methanococcus maripaludis phosphoseryl-tRNA synthetase
journal, February 2007

  • Kamtekar, S.; Hohn, M. J.; Park, H.-S.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 8, p. 2620-2625
  • DOI: 10.1073/pnas.0611504104

Applications and statistics for multiple high-scoring segments in molecular sequences.
journal, June 1993

  • Karlin, S.; Altschul, S. F.
  • Proceedings of the National Academy of Sciences, Vol. 90, Issue 12, p. 5873-5877
  • DOI: 10.1073/pnas.90.12.5873

High-velocity microprojectiles for delivering nucleic acids into living cells
journal, May 1987

  • Klein, T. M.; Wolf, E. D.; Wu, R.
  • Nature, Vol. 327, Issue 6117, p. 70-73
  • DOI: 10.1038/327070a0

Purification and characterization of IL6-PE4e, a recombinant fusion of interleukin 6 with Pseudomonas exotoxin
journal, November 1993

  • Kreitman, Robert J.; Pastan, Ira
  • Bioconjugate Chemistry, Vol. 4, Issue 6, p. 581-585
  • DOI: 10.1021/bc00024a025

Uniform Binding of Aminoacyl-tRNAs to Elongation Factor Tu by Thermodynamic Compensation
journal, October 2001

  • LaRiviere, Frederick J.; Wolfson, Alexey D.; Uhlenbeck, Olke C.
  • Science, Vol. 294, Issue 5540, p. 165-168
  • DOI: 10.1126/science.1064242

Cysteinyl-tRNA formation: the last puzzle of aminoacyl-tRNA synthesis
journal, November 1999


Usage of an intronic promoter for stable gene expression in Saccharomyces cerevisiae
journal, May 2005


Pathogenic mechanism of a human mitochondrial tRNAPhe mutation associated with myoclonic epilepsy with ragged red fibers syndrome
journal, September 2007

  • Ling, J.; Roy, H.; Qin, D.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 39, p. 15299-15304
  • DOI: 10.1073/pnas.0704441104

Phenylalanyl-tRNA synthetase editing defects result in efficient mistranslation of phenylalanine codons as tyrosine
journal, September 2007

  • Ling, J.; Yadavalli, S. S.; Ibba, M.
  • RNA, Vol. 13, Issue 11, p. 1881-1886
  • DOI: 10.1261/rna.684107

Synthesis of Cysteinyl-tRNACys by a Genome That Lacks the Normal Cysteine-tRNA Synthetase
journal, July 2000

  • Lipman, Richard S. A.; Sowers, Kevin R.; Hou, Ya-Ming
  • Biochemistry, Vol. 39, Issue 26, p. 7792-7798
  • DOI: 10.1021/bi0004955

Adenovirus tripartite leader sequence enhances translation of mRNAs late after infection.
journal, June 1984

  • Logan, J.; Shenk, T.
  • Proceedings of the National Academy of Sciences, Vol. 81, Issue 12, p. 3655-3659
  • DOI: 10.1073/pnas.81.12.3655

Probability-Based Validation of Protein Identifications Using a Modified SEQUEST Algorithm
journal, November 2002

  • MacCoss, Michael J.; Wu, Christine C.; Yates, John R.
  • Analytical Chemistry, Vol. 74, Issue 21, p. 5593-5599
  • DOI: 10.1021/ac025826t

PSI-BLAST-ISS: an intermediate sequence search tool for estimation of the position-specific alignment reliability
journal, January 2005

  • Margelevičius, Mindaugas; Venclovas, Česlovas
  • BMC Bioinformatics, Vol. 6, Issue 1, Article No. 185
  • DOI: 10.1186/1471-2105-6-185

Transfer RNA-dependent amino acid biosynthesis: An essential route to asparagine formation
journal, March 2002

  • Min, B.; Pelaschier, J. T.; Graham, D. E.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 5, p. 2678-2683
  • DOI: 10.1073/pnas.012027399

A novel O-phospho-L-serine sulfhydrylation reaction catalyzed by O-acetylserine sulfhydrylase from Aeropyrum pernix K1
journal, August 2003


Possible incorporation of phosphoserine into globin readthrough protein via bovine opal suppressor phosphoseryl-tRNA
journal, October 1986


Crystal Structure of the Ternary Complex of Phe-tRNAPhe, EF-Tu, and a GTP Analog
journal, December 1995


Changing the identity of a transfer RNA
journal, May 1986

  • Normanly, Jennifer; Ogden, Richard C.; Horvath, Suzanna J.
  • Nature, Vol. 321, Issue 6067, p. 213-219
  • DOI: 10.1038/321213a0

Design and Evolution of New Catalytic Activity with an Existing Protein Scaffold
journal, January 2006

  • Park, Hee-Sung; Nam, Sung-Hun; Lee, Jin Kak
  • Science, Vol. 311, Issue 5760, p. 535-538
  • DOI: 10.1126/science.1118953

Expanding the Genetic Code of Escherichia coli with Phosphoserine
journal, August 2011


Improved tools for biological sequence comparison.
journal, April 1988

  • Pearson, W. R.; Lipman, D. J.
  • Proceedings of the National Academy of Sciences, Vol. 85, Issue 8, p. 2444-2448
  • DOI: 10.1073/pnas.85.8.2444

Activation of the Pyrrolysine Suppressor tRNA Requires Formation of a Ternary Complex with Class I and Class II Lysyl-tRNA Synthetases
journal, August 2003


An aminoacyl-tRNA synthetase that specifically activates pyrrolysine
journal, August 2004

  • Polycarpo, C.; Ambrogelly, A.; Berube, A.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 34, p. 12450-12454
  • DOI: 10.1073/pnas.0405362101

Caged Phosphoproteins
journal, January 2005

  • Rothman, Deborah M.; Petersson, E. James; Vázquez, M. Eugenio
  • Journal of the American Chemical Society, Vol. 127, Issue 3, p. 846-847
  • DOI: 10.1021/ja043875c

Cysteinyl-tRNACys Formation in Methanocaldococcus jannaschii: the Mechanism Is Still Unknown
journal, December 2003


A Hypergeometric Probability Model for Protein Identification and Validation Using Tandem Mass Spectral Data and Protein Sequence Databases
journal, August 2003

  • Sadygov, Rovshan G.; Yates, John R.
  • Analytical Chemistry, Vol. 75, Issue 15, p. 3792-3798
  • DOI: 10.1021/ac034157w

Gene Transfer into Hepatocytes and Human Liver Tissue by Baculovirus Vectors
journal, October 1996

  • Sandig, Volker; Hofmann, Christian; Steinert, Susanne
  • Human Gene Therapy, Vol. 7, Issue 16, p. 1937-1945
  • DOI: 10.1089/hum.1996.7.16-1937

RNA-Dependent Cysteine Biosynthesis in Archaea
journal, March 2005

  • Sauerwald, Anselm; Zhu, Wenhong; Major, Tiffany A.
  • Science, Vol. 307, Issue 5717, p. 1969-1972
  • DOI: 10.1126/science.1108329

Understanding the Sequence Specificity of tRNA Binding to Elongation Factor Tu using tRNA Mutagenesis
journal, March 2009

  • Schrader, Jared M.; Chapman, Stephen J.; Uhlenbeck, Olke C.
  • Journal of Molecular Biology, Vol. 386, Issue 5, p. 1255-1264
  • DOI: 10.1016/j.jmb.2009.01.021

Targeting the mitogen-activated protein kinase cascade to treat cancer
journal, December 2004

  • Sebolt-Leopold, Judith S.; Herrera, Roman
  • Nature Reviews Cancer, Vol. 4, Issue 12, p. 937-947
  • DOI: 10.1038/nrc1503

Cysteinyl-tRNA synthetase is not essential for viability of the archaeon Methanococcus maripaludis
journal, November 2001

  • Stathopoulos, C.; Kim, W.; Li, T.
  • Proceedings of the National Academy of Sciences, Vol. 98, Issue 25, p. 14292-14297
  • DOI: 10.1073/pnas.201540498

One Polypeptide with Two Aminoacyl-tRNA Synthetase Activities
journal, January 2000


Protein production by auto-induction in high-density shaking cultures
journal, May 2005


DTASelect and Contrast:  Tools for Assembling and Comparing Protein Identifications from Shotgun Proteomics
journal, February 2002

  • Tabb, David L.; McDonald, W. Hayes; Yates, John R.
  • Journal of Proteome Research, Vol. 1, Issue 1, p. 21-26
  • DOI: 10.1021/pr015504q

The role of tightly bound ATP in Escherichia coli tRNA nucleotidyltransferase
journal, September 2000


Transformation of Methanococcus maripaludis and identification of a Pst I-like restriction system
journal, September 1994


Expanding the Genetic Code of Escherichia coli
journal, April 2001


The biosynthesis of cysteine and homocysteine in Methanococcus jannaschii
journal, December 2003


Isolation and characterization of 22 mesophilic methanococci
journal, May 1986

  • Whitman, William B.; Shieh, Jersong; Sohn, Seehyang
  • Systematic and Applied Microbiology, Vol. 7, Issue 2-3, p. 235-240
  • DOI: 10.1016/S0723-2020(86)80012-1

Modulation of tRNAAla identity by inorganic pyrophosphatase
journal, April 2002

  • Wolfson, A. D.; Uhlenbeck, O. C.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 9, p. 5965-5970
  • DOI: 10.1073/pnas.092152799

Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNACys
journal, April 2008

  • Zhang, Chun-Mei; Liu, Cuiping; Slater, Simon
  • Nature Structural & Molecular Biology, Vol. 15, Issue 5, p. 507-514
  • DOI: 10.1038/nsmb.1423

Shotgun Proteomics of Methanococcus jannaschii and Insights into Methanogenesis
journal, June 2004

  • Zhu, Wenhong; Reich, Claudia I.; Olsen, Gary J.
  • Journal of Proteome Research, Vol. 3, Issue 3, p. 538-548
  • DOI: 10.1021/pr034109s

Crystal Structure of Formate Dehydrogenase H: Catalysis Involving Mo, Molybdopterin, Selenocysteine, and an Fe4S4 Cluster
journal, February 1997

  • Boyington, Jeffrey C.; Gladyshev, Vadim N.; Khangulov, Sergei V.
  • Science, Vol. 275, Issue 5304, p. 1305-1308
  • DOI: 10.1126/science.275.5304.1305

Transfer RNAs That Insert Selenocysteine
book, January 2002

  • Carlson, Bradley A.; Hatfield, Dolph L.
  • Protein Sensors and Reactive Oxygen Species - Part A: Selenoproteins and Thioredoxin, p. 24-39
  • DOI: 10.1016/S0076-6879(02)47005-X

Structural insights into the first step of RNA-dependent cysteine biosynthesis in archaea
journal, March 2007

  • Fukunaga, Ryuya; Yokoyama, Shigeyuki
  • Nature Structural & Molecular Biology, Vol. 14, Issue 4, p. 272-279
  • DOI: 10.1038/nsmb1219

An engineered selenocysteine defines a unique class of antibody derivatives
journal, August 2008

  • Hofer, T.; Thomas, J. D.; Burke, T. R.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 34, p. 12451-12456
  • DOI: 10.1073/pnas.0800800105

Five-base codons for incorporation of nonnatural amino acids into proteins
journal, September 2001

  • Hohsaka, Takahiro; Ashizuka, Yuki; Murakami, Hiroshi
  • Nucleic Acids Research, Vol. 29, Issue 17, p. 3646-3651
  • DOI: 10.1093/nar/29.17.3646

Sulfur and Selenium: The Role of Oxidation State in Protein Structure and Function
journal, October 2003

  • Jacob, Claus; Giles, Gregory I.; Giles, Niroshini M.
  • Angewandte Chemie International Edition, Vol. 42, Issue 39, p. 4742-4758
  • DOI: 10.1002/anie.200300573

Selenocysteine in proteins—properties and biotechnological use
journal, October 2005

  • Johansson, Linda; Gafvelin, Guro; Arnér, Elias S.J.
  • Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1726, Issue 1, p. 1-13
  • DOI: 10.1016/j.bbagen.2005.05.010

Characterization of Mammalian Selenoproteomes
journal, May 2003

  • Kryukov, Gregory V.; Castellano, Sergi; Novoselov, Sergey V.
  • Science, Vol. 300, Issue 5624, p. 1439-1443
  • DOI: 10.1126/science.1083516

The discriminator base influences tRNA structure at the end of the acceptor stem and possibly its interaction with proteins.
journal, August 1993

  • Lee, C. P.; Mandal, N.; Dyson, M. R.
  • Proceedings of the National Academy of Sciences, Vol. 90, Issue 15, p. 7149-7152
  • DOI: 10.1073/pnas.90.15.7149

Catalytic Mechanism of Sep-tRNA:Cys-tRNA Synthase: Sulfur Transfer Is Mediated By Disulfide And Persulfide
journal, December 2011

  • Liu, Yuchen; Dos Santos, Patricia C.; Zhu, Xiang
  • Journal of Biological Chemistry, Vol. 287, Issue 8, p. 5426-5433
  • DOI: 10.1074/jbc.M111.313700

The Human SepSecS-tRNASec Complex Reveals the Mechanism of Selenocysteine Formation
journal, July 2009

  • Palioura, S.; Sherrer, R. L.; Steitz, T. A.
  • Science, Vol. 325, Issue 5938, p. 321-325
  • DOI: 10.1126/science.1173755

Diselenium, instead of disulfide, bonded analogs of conotoxins: novel synthesis and pharmacotherapeutic potential
journal, October 2010


The selenocysteine-inserting opal suppressor serine tRNA from E.coli is highly unusual in structure and modification
journal, January 1989

  • Schön, Astrid; Böck, August; Ott, Günther
  • Nucleic Acids Research, Vol. 17, Issue 18, p. 7159-7165
  • DOI: 10.1093/nar/17.18.7159

Identification and characterization of a selenoprotein family containing a diselenide bond in a redox motif
journal, August 2007

  • Shchedrina, V. A.; Novoselov, S. V.; Malinouski, M. Yu.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 35, p. 13919-13924
  • DOI: 10.1073/pnas.0703448104

Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation
journal, January 2008

  • Sherrer, R. Lynn; O’Donoghue, Patrick; Söll, Dieter
  • Nucleic Acids Research, Vol. 36, Issue 4, p. 1247-1259
  • DOI: 10.1093/nar/gkm1134

Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O -phosphoseryl-tRNA Sec kinase
journal, February 2008

  • Sherrer, R. Lynn; Ho, Joanne M. L.; Söll, Dieter
  • Nucleic Acids Research, Vol. 36, Issue 6, p. 1871-1880
  • DOI: 10.1093/nar/gkn036

The antioxidant role of selenium and seleno-compounds
journal, May 2003


New Developments in Selenium Biochemistry: Selenocysteine Biosynthesis in Eukaryotes and Archaea
journal, August 2007

  • Xu, Xue-Ming; Carlson, Bradley A.; Zhang, Yan
  • Biological Trace Element Research, Vol. 119, Issue 3, p. 234-241
  • DOI: 10.1007/s12011-007-8003-9

The many levels of control on bacterial selenoprotein synthesis
journal, November 2009

  • Yoshizawa, Satoko; Böck, August
  • Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1790, Issue 11, p. 1404-1414
  • DOI: 10.1016/j.bbagen.2009.03.010

Distinct genetic code expansion strategies for selenocysteine and pyrrolysine are reflected in different aminoacyl-tRNA formation systems
journal, November 2009


RNA-dependent conversion of phosphoserine forms selenocysteine in eukaryotes and archaea
journal, December 2006

  • Yuan, J.; Palioura, S.; Salazar, J. C.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 50, p. 18923-18927
  • DOI: 10.1073/pnas.0609703104

Probing the role of the proximal heme ligand in cytochrome P450cam by recombinant incorporation of selenocysteine
journal, March 2009

  • Aldag, C.; Gromov, I. A.; Garcia-Rubio, I.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 14, p. 5481-5486
  • DOI: 10.1073/pnas.0810503106

Pyrrolysine is not hardwired for cotranslational insertion at UAG codons
journal, February 2007

  • Ambrogelly, A.; Gundllapalli, S.; Herring, S.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 9, p. 3141-3146
  • DOI: 10.1073/pnas.0611634104

Recombinant Expression of Mammalian Selenocysteine-Containing Thioredoxin Reductase and Other Selenoproteins in Escherichia coli
book, January 2002


Selenoproteins—What unique properties can arise with selenocysteine in place of cysteine?
journal, May 2010


Stationary-phase expression and aminoacylation of a transfer-RNA-like small RNA
journal, August 2005

  • Ataide, Sandro F.; Jester, Brian C.; Devine, Kevin M.
  • EMBO reports, Vol. 6, Issue 8, p. 742-747
  • DOI: 10.1038/sj.embor.7400474

Ribosome-mediated incorporation of a non-standard amino acid into a peptide through expansion of the genetic code
journal, April 1992

  • Bain, J. D.; Switzer, Christopher; Chamberlin, Richard
  • Nature, Vol. 356, Issue 6369, p. 537-539
  • DOI: 10.1038/356537a0

Selenoglutathione:  Efficient Oxidative Protein Folding by a Diselenide
journal, May 2007

  • Beld, Joris; Woycechowsky, Kenneth J.; Hilvert, Donald
  • Biochemistry, Vol. 46, Issue 18, p. 5382-5390
  • DOI: 10.1021/bi700124p

The 2.9 A crystal structure of T. thermophilus seryl-tRNA synthetase complexed with tRNA(Ser)
journal, March 1994


Recoding the Genetic Code with Selenocysteine
journal, November 2013

  • Bröcker, Markus J.; Ho, Joanne M. L.; Church, George M.
  • Angewandte Chemie International Edition, Vol. 53, Issue 1, p. 319-323
  • DOI: 10.1002/anie.201308584

UGA is an additional glycine codon in uncultured SR1 bacteria from the human microbiota
journal, March 2013

  • Campbell, J. H.; O'Donoghue, P.; Campbell, A. G.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 14, p. 5540-5545
  • DOI: 10.1073/pnas.1303090110

Post-transcriptional nucleotide addition is responsible for the formation of the 5' terminus of histidine tRNA
journal, November 1982

  • Cooley, L.; Appel, B.; Soll, D.
  • Proceedings of the National Academy of Sciences, Vol. 79, Issue 21, p. 6475-6479
  • DOI: 10.1073/pnas.79.21.6475

Manipulating Cellular Activities Using an Ultrasound–Chemical Hybrid Tool
journal, August 2017

  • Fan, Ching-Hsiang; Huang, Yao-Shen; Huang, Wei-En
  • ACS Synthetic Biology, Vol. 6, Issue 11, p. 2021-2027
  • DOI: 10.1021/acssynbio.7b00162

Efficient Expression of Glutathione Peroxidase with Chimeric tRNA in Amber-less Escherichia coli
journal, August 2017


The pathway to GTPase activation of elongation factor SelB on the ribosome
journal, November 2016

  • Fischer, Niels; Neumann, Piotr; Bock, Lars V.
  • Nature, Vol. 540, Issue 7631, p. 80-85
  • DOI: 10.1038/nature20560

Reconstitution of Selenocysteine Incorporation Reveals Intrinsic Regulation by SECIS Elements
journal, July 2013

  • Gupta, Nirupama; DeMong, Louise W.; Banda, Sowmya
  • Journal of Molecular Biology, Vol. 425, Issue 14, p. 2415-2422
  • DOI: 10.1016/j.jmb.2013.04.016

Alternative genetic code for amino acids and transfer RNA revisited
journal, January 2013

  • Hamashima, Kiyofumi; Kanai, Akio
  • BioMolecular Concepts, Vol. 4, Issue 3, p. 309-318
  • DOI: 10.1515/bmc-2013-0002

Expansion of Noncanonical V-Arm-Containing tRNAs in Eukaryotes
journal, November 2015

  • Hamashima, Kiyofumi; Tomita, Masaru; Kanai, Akio
  • Molecular Biology and Evolution, Vol. 33, Issue 2, p. 530-540
  • DOI: 10.1093/molbev/msv253

Engineering the elongation factor Tu for efficient selenoprotein synthesis
journal, July 2014

  • Haruna, Ken-ichi; Alkazemi, Muhammad H.; Liu, Yuchen
  • Nucleic Acids Research, Vol. 42, Issue 15, p. 9976-9983
  • DOI: 10.1093/nar/gku691

Conversion of aminoacylation specificity from tRNATyr to tRNASer in vitro
journal, January 1990

  • Himeno, Hyouta; Hasegawa, Tsunemi; Ueda, Takuya
  • Nucleic Acids Research, Vol. 18, Issue 23, p. 6815-6819
  • DOI: 10.1093/nar/18.23.6815

A simple structural feature is a major determinant of the identity of a transfer RNA
journal, May 1988

  • Hou, Ya-Ming; Schimmel, Paul
  • Nature, Vol. 333, Issue 6169, p. 140-145
  • DOI: 10.1038/333140a0

The 9/4 secondary structure of eukaryotic selenocysteine tRNA: More pieces of evidence
journal, September 1998


Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement
journal, July 2011

  • Isaacs, Farren J.; Carr, Peter A.; Wang, Harris H.
  • Science, Vol. 333, Issue 6040, p. 348-353
  • DOI: 10.1126/science.1205822

Crystal structure of human selenocysteine tRNA
journal, August 2009

  • Itoh, Yuzuru; Chiba, Shiho; Sekine, Shun-ichi
  • Nucleic Acids Research, Vol. 37, Issue 18, p. 6259-6268
  • DOI: 10.1093/nar/gkp648

Tertiary structure of bacterial selenocysteine tRNA
journal, May 2013

  • Itoh, Y.; Sekine, S.-i.; Suetsugu, S.
  • Nucleic Acids Research, Vol. 41, Issue 13, p. 6729-6738
  • DOI: 10.1093/nar/gkt321

Decameric SelA•tRNASec Ring Structure Reveals Mechanism of Bacterial Selenocysteine Formation
journal, April 2013


Stop codon reassignments in the wild
journal, May 2014

  • Ivanova, N. N.; Schwientek, P.; Tripp, H. J.
  • Science, Vol. 344, Issue 6186, p. 909-913
  • DOI: 10.1126/science.1250691

An integrated cell-free metabolic platform for protein production and synthetic biology
journal, October 2008

  • Jewett, Michael C.; Calhoun, Kara A.; Voloshin, Alexei
  • Molecular Systems Biology, Vol. 4, Issue 1, Article No. 220
  • DOI: 10.1038/msb.2008.57

Ribosomal synthesis of nonstandard peptides
journal, April 2008

  • Kang, Taek Jin; Suga, Hiroaki
  • Biochemistry and Cell Biology, Vol. 86, Issue 2, p. 92-99
  • DOI: 10.1139/O08-009

Non-canonical roles of tRNAs and tRNA mimics in bacterial cell biology
journal, June 2016

  • Katz, Assaf; Elgamal, Sara; Rajkovic, Andrei
  • Molecular Microbiology, Vol. 101, Issue 4, p. 545-558
  • DOI: 10.1111/mmi.13419

Selenium utilization in thioredoxin and catalytic advantage provided by selenocysteine
journal, June 2015

  • Kim, Moon-Jung; Lee, Byung Cheon; Hwang, Kwang Yeon
  • Biochemical and Biophysical Research Communications, Vol. 461, Issue 4, p. 648-652
  • DOI: 10.1016/j.bbrc.2015.04.082

Recognition of tRNACys by Escherichia coli cysteinyl-tRNA synthetase
journal, July 1993

  • Komatsoulis, George A.; Abelson, John
  • Biochemistry, Vol. 32, Issue 29, p. 7435-7444
  • DOI: 10.1021/bi00080a014

Selenite is a substrate for calf thymus thioredoxin reductase and thioredoxin and elicits a large non-stoichiometric oxidation of NADPH in the presence of oxygen
journal, July 1992


Direct detection of potential selenium delivery proteins by using an Escherichia coli strain unable to incorporate selenium from selenite into proteins
journal, June 2002

  • Lacourciere, G. M.; Levine, R. L.; Stadtman, T. C.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 14, p. 9150-9153
  • DOI: 10.1073/pnas.142291199

Genomically Recoded Organisms Expand Biological Functions
journal, October 2013

  • Lajoie, M. J.; Rovner, A. J.; Goodman, D. B.
  • Science, Vol. 342, Issue 6156, p. 357-360
  • DOI: 10.1126/science.1241459

Clustal W and Clustal X version 2.0
journal, September 2007


ARAGORN, a program to detect tRNA genes and tmRNA genes in nucleotide sequences
journal, January 2004

  • Laslett, Dean; Canback, Bjorn
  • Nucleic Acids Research, Vol. 32, Issue 1, p. 11-16
  • DOI: 10.1093/nar/gkh152

Adding New Chemistries to the Genetic Code
journal, June 2010


Adaptation to tRNA acceptor stem structure by flexible adjustment in the catalytic domain of class I tRNA synthetases
journal, December 2011


Silvibacterium bohemicum gen. nov. sp. nov., an acidobacterium isolated from coniferous soil in the Bohemian Forest National Park
journal, February 2016

  • Lladó, Salvador; Benada, Oldrich; Cajthaml, Tomáš
  • Systematic and Applied Microbiology, Vol. 39, Issue 1, p. 14-19
  • DOI: 10.1016/j.syapm.2015.12.005

A semi-synthetic organism with an expanded genetic alphabet
journal, May 2014

  • Malyshev, Denis A.; Dhami, Kirandeep; Lavergne, Thomas
  • Nature, Vol. 509, Issue 7500, p. 385-388
  • DOI: 10.1038/nature13314

IMG/M 4 version of the integrated metagenome comparative analysis system
journal, October 2013

  • Markowitz, Victor M.; Chen, I-Min A.; Chu, Ken
  • Nucleic Acids Research, Vol. 42, Issue D1, p. D568-D573
  • DOI: 10.1093/nar/gkt919

Expression of synthetic suppressor tRNA genes under the control of a synthetic promoter
journal, January 1986


Changing the identity of a tRNA by introducing a G-U wobble pair near the 3' acceptor end
journal, May 1988


Efficiency of Mammalian Selenocysteine Incorporation
journal, June 2004

  • Mehta, Anupama; Rebsch, Cheryl M.; Kinzy, Scott A.
  • Journal of Biological Chemistry, Vol. 279, Issue 36, p. 37852-37859
  • DOI: 10.1074/jbc.M404639200

Fast purification of a functional elongator tRNAmet expressed from a synthetic gene in vivo
journal, January 1988

  • Meinnel, Thierry; Mechulam, Yves; Fayat, Guy
  • Nucleic Acids Research, Vol. 16, Issue 16, p. 8095-8112
  • DOI: 10.1093/nar/16.16.8095

A synthetic tRNA for EF-Tu mediated selenocysteine incorporation in vivo and in vitro
journal, July 2015


The dual identities of mammalian tRNASec for SerRS and selenocysteine synthase
journal, January 1998

  • Mizutani, Takaharu; Kanaya, Kazuo; Ikeda, Seiei
  • Molecular Biology Reports, Vol. 25, Issue 4, p. 211-216
  • DOI: 10.1023/A:1006879820805

A novel nuclear genetic code alteration in yeasts and the evolution of codon reassignment in eukaryotes
journal, May 2016

  • Mühlhausen, Stefanie; Findeisen, Peggy; Plessmann, Uwe
  • Genome Research, Vol. 26, Issue 7, p. 945-955
  • DOI: 10.1101/gr.200931.115

Facile Recoding of Selenocysteine in Nature
journal, March 2016

  • Mukai, Takahito; Englert, Markus; Tripp, H. James
  • Angewandte Chemie International Edition, Vol. 55, Issue 17, p. 5337-5341
  • DOI: 10.1002/anie.201511657

RNA-Dependent Cysteine Biosynthesis in Bacteria and Archaea
journal, May 2017

  • Mukai, Takahito; Crnković, Ana; Umehara, Takuya
  • mBio, Vol. 8, Issue 3, Article No. e00561-17
  • DOI: 10.1128/mBio.00561-17

Noncanonical Secondary Structure Stabilizes Mitochondrial tRNASer(UCN) by Reducing the Entropic Cost of Tertiary Folding
journal, March 2015

  • Mustoe, Anthony M.; Liu, Xin; Lin, Paul J.
  • Journal of the American Chemical Society, Vol. 137, Issue 10, p. 3592-3599
  • DOI: 10.1021/ja5130308

The selective tRNA aminoacylation mechanism based on a single G•U pair
journal, June 2014

  • Naganuma, Masahiro; Sekine, Shun-ichi; Chong, Yeeting Esther
  • Nature, Vol. 510, Issue 7506, p. 507-511
  • DOI: 10.1038/nature13440

Pyrrolysyl-tRNA synthetase–tRNAPyl structure reveals the molecular basis of orthogonality
journal, December 2008

  • Nozawa, Kayo; O’Donoghue, Patrick; Gundllapalli, Sarath
  • Nature, Vol. 457, Issue 7233, p. 1163-1167
  • DOI: 10.1038/nature07611

The additional guanylate at the 5' terminus of Escherichia coli tRNAHis is the result of unusual processing by RNase P.
journal, February 1986

  • Orellana, O.; Cooley, L.; Söll, D.
  • Molecular and Cellular Biology, Vol. 6, Issue 2, p. 525-529
  • DOI: 10.1128/MCB.6.2.525

Thermodynamic and Kinetic Framework of Selenocysteyl-tRNASec Recognition by Elongation Factor SelB
journal, November 2009

  • Paleskava, Alena; Konevega, Andrey L.; Rodnina, Marina V.
  • Journal of Biological Chemistry, Vol. 285, Issue 5, p. 3014-3020
  • DOI: 10.1074/jbc.M109.081380

Computational identification of the selenocysteine tRNA (tRNASec) in genomes
journal, February 2017

  • Santesmasses, Didac; Mariotti, Marco; Guigó, Roderic
  • PLOS Computational Biology, Vol. 13, Issue 2, Article No. e1005383
  • DOI: 10.1371/journal.pcbi.1005383

Artificial lantipeptides from in vitro translations
journal, January 2011

  • Seebeck, Florian P.; Ricardo, Alonso; Szostak, Jack W.
  • Chemical Communications, Vol. 47, Issue 21, p. 6141-6143
  • DOI: 10.1039/c0cc05663d

Formation of a Ternary Complex for Selenocysteine Biosynthesis in Bacteria
journal, September 2015

  • Silva, Ivan R.; Serrão, Vitor H. B.; Manzine, Livia R.
  • Journal of Biological Chemistry, Vol. 290, Issue 49, p. 29178-29188
  • DOI: 10.1074/jbc.M114.613406

Compilation of tRNA sequences and sequences of tRNA genes
journal, January 1998

  • Sprinzl, Mathias; Horn, Carsten; Brown, Melissa
  • Nucleic Acids Research, Vol. 26, Issue 1, p. 148-153
  • DOI: 10.1093/nar/26.1.148

The ‘polysemous’ codon—a codon with multiple amino acid assignment caused by dual specificity of tRNA identity
journal, March 1997

  • Suzuki, Tsutomu; Ueda, Takuya; Watanabe, Kimitsuna
  • The EMBO Journal, Vol. 16, Issue 5, p. 1122-1134
  • DOI: 10.1093/emboj/16.5.1122

Genetic Codes with No Dedicated Stop Codon: Context-Dependent Translation Termination
journal, July 2016


Selenite Reduction by the Thioredoxin System: Kinetics and Identification of Protein-Bound Selenide
journal, June 2011

  • Tamura, Takashi; Sato, Kumi; Komori, Kentaro
  • Bioscience, Biotechnology, and Biochemistry, Vol. 75, Issue 6, p. 1184-1187
  • DOI: 10.1271/bbb.100847

Evolving tRNASec for Efficient Canonical Incorporation of Selenocysteine
journal, December 2014

  • Thyer, Ross; Robotham, Scott A.; Brodbelt, Jennifer S.
  • Journal of the American Chemical Society, Vol. 137, Issue 1, p. 46-49
  • DOI: 10.1021/ja510695g

Genetic Code Supports Targeted Insertion of Two Amino Acids by One Codon
journal, January 2009

  • Turanov, A. A.; Lobanov, A. V.; Fomenko, D. E.
  • Science, Vol. 323, Issue 5911, p. 259-261
  • DOI: 10.1126/science.1164748

Wobble decoding by the Escherichia coli selenocysteine insertion machinery
journal, August 2013

  • Xu, J.; Croitoru, V.; Rutishauser, D.
  • Nucleic Acids Research, Vol. 41, Issue 21, p. 9800-9811
  • DOI: 10.1093/nar/gkt764

Biosynthesis of Selenocysteine on Its tRNA in Eukaryotes
journal, December 2006