Systems, devices, and methods for agglutination assays using sedimentation

Title: Systems, devices, and methods for agglutination assays using sedimentation

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Abstract

Embodiments of the present invention include methods for conducting agglutination assays using sedimentation. Aggregates may be exposed to sedimentation forces and travel through a density medium to a detection area. Microfluidic devices, such as microfluidic disks, are described for conducting the agglutination assays, as are systems for conducting the assays.

Inventors:: Schaff, Ulrich Y.; Sommer, Gregory Jon; Singh, Anup K.

Issue Date:: 2018-07-17

Research Org.:: Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1465194

Patent Number(s):: 10024849

Application Number:: 14/853,660

Assignee:: National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM)

Patent Classifications (CPCs):: B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01D - SEPARATION

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
B01L2400/0457 - passive flow or gravitation
B01L3/5021 - {Test tubes specially adapted for centrifugation purposes
B01L3/502761 - {specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01D - SEPARATION
B01D21/262 - {by using a centrifuge}

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
B01L2300/0861 - Configuration of multiple channels and/or chambers in a single devices
B01L2400/0409 - centrifugal forces

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N33/543 - with an insoluble carrier for immobilising immunochemicals

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
B01L2200/0647 - Handling flowable solids, e.g. microscopic beads, cells, particles
B01L2300/0803 - Disc shape

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N33/537 - with separation of immune complex from unbound antigen or antibody
G01N33/538 - by sorbent column, particles or resin strip {, i.e. sorbent materials}

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
B01L2200/0631 - Purification arrangements, e.g. solid phase extraction [SPE]
B01L2300/0627 - Sensor or part of a sensor is integrated

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N33/54366 - {Apparatus specially adapted for solid-phase testing}

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
B01L3/502715 - {characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces}
B01L2200/10 - Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N33/54313 - {the carrier being characterised by its particulate form}

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
B01L3/50215 - {using a float to separate phases}
B01L3/502753 - {characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
B01L3/502738 - {characterised by integrated valves

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DOE Contract Number:: AC04-94AL85000

Resource Type:: Patent

Resource Relation:: Patent File Date: 2015 Sep 14

Country of Publication:: United States

Language:: English

Citation Formats


                    Schaff, Ulrich Y., Sommer, Gregory Jon, and Singh, Anup K. Systems, devices, and methods for agglutination assays using sedimentation.  United States: N. p., 2018. 
        Web.

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                    Schaff, Ulrich Y., Sommer, Gregory Jon, & Singh, Anup K. Systems, devices, and methods for agglutination assays using sedimentation.  United States.

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                    Schaff, Ulrich Y., Sommer, Gregory Jon, and Singh, Anup K. Tue .  
        "Systems, devices, and methods for agglutination assays using sedimentation".  United States.  https://www.osti.gov/servlets/purl/1465194.

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@article{osti_1465194,

  title        = {Systems, devices, and methods for agglutination assays using sedimentation},

  author       = {Schaff, Ulrich Y. and Sommer, Gregory Jon and Singh, Anup K.},

  abstractNote = {Embodiments of the present invention include methods for conducting agglutination assays using sedimentation. Aggregates may be exposed to sedimentation forces and travel through a density medium to a detection area. Microfluidic devices, such as microfluidic disks, are described for conducting the agglutination assays, as are systems for conducting the assays.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2018},

  month        = {7}

}

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