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Title: Enhanced processive cellulases

Abstract

Nucleic acid sequences encoding chimeric polypeptides that exhibit enhanced cellulase activities are disclosed herein. These nucleic acids may be expressed in hosts such as fungi, which in turn may be cultured to produce chimeric polypeptides. Also disclosed are chimeric polypeptides and their use in the degradation of cellulosic materials.

Inventors:
; ; ; ; ; ; ; ; ; ;
Issue Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1464739
Patent Number(s):
10,036,051
Application Number:
15/594,031
Assignee:
Alliance for Sustainable Energy, LLC (Golden, CO)
DOE Contract Number:  
AC36-08GO28308
Resource Type:
Patent
Resource Relation:
Patent File Date: 2017 May 12
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Adney, William S., Beckham, Gregg T., Jarvis, Eric, Himmel, Michael E., Decker, Stephen R., Linger, Jeffrey G., Podkaminer, Kara, Baker, John O., Taylor, II, Larry, Xu, Qi, and Singh, Arjun. Enhanced processive cellulases. United States: N. p., 2018. Web.
Adney, William S., Beckham, Gregg T., Jarvis, Eric, Himmel, Michael E., Decker, Stephen R., Linger, Jeffrey G., Podkaminer, Kara, Baker, John O., Taylor, II, Larry, Xu, Qi, & Singh, Arjun. Enhanced processive cellulases. United States.
Adney, William S., Beckham, Gregg T., Jarvis, Eric, Himmel, Michael E., Decker, Stephen R., Linger, Jeffrey G., Podkaminer, Kara, Baker, John O., Taylor, II, Larry, Xu, Qi, and Singh, Arjun. Tue . "Enhanced processive cellulases". United States. https://www.osti.gov/servlets/purl/1464739.
@article{osti_1464739,
title = {Enhanced processive cellulases},
author = {Adney, William S. and Beckham, Gregg T. and Jarvis, Eric and Himmel, Michael E. and Decker, Stephen R. and Linger, Jeffrey G. and Podkaminer, Kara and Baker, John O. and Taylor, II, Larry and Xu, Qi and Singh, Arjun},
abstractNote = {Nucleic acid sequences encoding chimeric polypeptides that exhibit enhanced cellulase activities are disclosed herein. These nucleic acids may be expressed in hosts such as fungi, which in turn may be cultured to produce chimeric polypeptides. Also disclosed are chimeric polypeptides and their use in the degradation of cellulosic materials.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {7}
}

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Works referenced in this record:

Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design
journal, August 2005

  • Chica, Roberto A.; Doucet, Nicolas; Pelletier, Joelle N.
  • Current Opinion in Biotechnology, Vol. 16, Issue 4, p. 378-384
  • DOI: 10.1016/j.copbio.2005.06.004

Bioengineering for Microbial Inulinases: Trends and Applications
journal, July 2017


A family of thermostable fungal cellulases created by structure-guided recombination
journal, March 2009

  • Heinzelman, P.; Snow, C. D.; Wu, I.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 14, p. 5610-5615
  • DOI: 10.1073/pnas.0901417106

SCHEMA Recombination of a Fungal Cellulase Uncovers a Single Mutation That Contributes Markedly to Stability
journal, July 2009

  • Heinzelman, Pete; Snow, Christopher D.; Smith, Matthew A.
  • Journal of Biological Chemistry, Vol. 284, Issue 39, p. 26229-26233
  • DOI: 10.1074/jbc.C109.034058

Binding modules alter the activity of chimeric cellulases: Effects of biomass pretreatment and enzyme source
journal, July 2010

  • Kim, Tae-Wan; Chokhawala, Harshal A.; Nadler, Dana
  • Biotechnology and Bioengineering, Vol. 107, Issue 4, p. 601-611
  • DOI: 10.1002/bit.22856

A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei
journal, January 1987


Developments in Directed Evolution for Improving Enzyme Functions
journal, August 2007

  • Sen, S.; Venkata Dasu, V.; Mandal, B.
  • Applied Biochemistry and Biotechnology, Vol. 143, Issue 3, p. 212-223
  • DOI: 10.1007/s12010-007-8003-4

Trichoderma reesei cellobiohydrolase I with an endoglucanase cellulose-binding domain: action on bacterial microcrystalline cellulose
journal, September 1997


Cellulases and biofuels
journal, June 2009