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Title: Enhanced processive cellulases

Abstract

Nucleic acid sequences encoding chimeric polypeptides that exhibit enhanced cellulase activities are disclosed herein. These nucleic acids may be expressed in hosts such as fungi, which in turn may be cultured to produce chimeric polypeptides. Also disclosed are chimeric polypeptides and their use in the degradation of cellulosic materials.

Inventors:
; ; ; ; ; ; ; ; ; ;
Issue Date:
Research Org.:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1464739
Patent Number(s):
10036051
Application Number:
15/594,031
Assignee:
Alliance for Sustainable Energy, LLC (Golden, CO)
Patent Classifications (CPCs):
C - CHEMISTRY C10 - PETROLEUM, GAS OR COKE INDUSTRIES C10L - FUELS NOT OTHERWISE PROVIDED FOR
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC36-08GO28308
Resource Type:
Patent
Resource Relation:
Patent File Date: 2017 May 12
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Adney, William S., Beckham, Gregg T., Jarvis, Eric, Himmel, Michael E., Decker, Stephen R., Linger, Jeffrey G., Podkaminer, Kara, Baker, John O., Taylor, II, Larry, Xu, Qi, and Singh, Arjun. Enhanced processive cellulases. United States: N. p., 2018. Web.
Adney, William S., Beckham, Gregg T., Jarvis, Eric, Himmel, Michael E., Decker, Stephen R., Linger, Jeffrey G., Podkaminer, Kara, Baker, John O., Taylor, II, Larry, Xu, Qi, & Singh, Arjun. Enhanced processive cellulases. United States.
Adney, William S., Beckham, Gregg T., Jarvis, Eric, Himmel, Michael E., Decker, Stephen R., Linger, Jeffrey G., Podkaminer, Kara, Baker, John O., Taylor, II, Larry, Xu, Qi, and Singh, Arjun. Tue . "Enhanced processive cellulases". United States. https://www.osti.gov/servlets/purl/1464739.
@article{osti_1464739,
title = {Enhanced processive cellulases},
author = {Adney, William S. and Beckham, Gregg T. and Jarvis, Eric and Himmel, Michael E. and Decker, Stephen R. and Linger, Jeffrey G. and Podkaminer, Kara and Baker, John O. and Taylor, II, Larry and Xu, Qi and Singh, Arjun},
abstractNote = {Nucleic acid sequences encoding chimeric polypeptides that exhibit enhanced cellulase activities are disclosed herein. These nucleic acids may be expressed in hosts such as fungi, which in turn may be cultured to produce chimeric polypeptides. Also disclosed are chimeric polypeptides and their use in the degradation of cellulosic materials.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {7}
}

Works referenced in this record:

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Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum
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Trichoderma reesei cellobiohydrolase I with an endoglucanase cellulose-binding domain: action on bacterial microcrystalline cellulose
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Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum
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A family of thermostable fungal cellulases created by structure-guided recombination
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Polypeptides having endoglucanase activity and polynucleotides encoding same
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SCHEMA Recombination of a Fungal Cellulase Uncovers a Single Mutation That Contributes Markedly to Stability
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Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
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Binding modules alter the activity of chimeric cellulases: Effects of biomass pretreatment and enzyme source
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Enhanced Processive Cellulases
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Developments in Directed Evolution for Improving Enzyme Functions
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Bioengineering for Microbial Inulinases: Trends and Applications
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Cellulases and biofuels
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