Mercury methylation genes in bacteria and archaea

Title: Mercury methylation genes in bacteria and archaea

Full Record
Similar

Two genes required for this mercury methylation have been identified in bacteria and archaea. These genes are the hgcA gene and hgcB, a corrinoid protein that facilitates methyl group transfer to Hg, and a corrinoid protein-associated ferredoxin with two [4Fe-4S] binding motifs involved in generating cob(I)almin, respectively. The invention provides nucleic acid probes and primers for detecting methylmercury and or for assessing mercury methylation potential in environmental, clinical and other samples. The invention also provides antibodies against these proteins, antibodies against these proteins, methods of using the antibodies and methods of biocatalysis.

Inventors:: Parks, Jerry M.; Johs, Alexander

Issue Date:: 2018-07-31

OSTI Identifier:: 1464626

Assignee:: UT-BATTELLE, LLC (Oak Ridge, TN) ORNL

Patent Number(s):: 10,036,072

Application Number:: 14/132,906

Contract Number:: AC05-00OR22725

Resource Relation:: Patent File Date: 2013 Dec 18

Research Org:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org:: USDOE

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Full Text
View Full Text

Similar records in DOepatents and OSTI.GOV collections:

Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor

Patent Maupin-Furlow, Julie A ; Talarico, Lee Ann ; Raj, Krishnan Chandra ; Ingram, Lonnie O

The invention provides isolated nucleic acids molecules which encode pyruvate decarboxylase enzymes having improved decarboxylase activity, substrate affinity, thermostability, and activity at different pH. The nucleic acids of the invention also have a codon usage which allows for high expression in a variety of host cells. Accordingly, the invention provides recombinant expression vectors containing such nucleic acid molecules, recombinant host cells comprising the expression vectors, host cells further comprising other ethanologenic enzymes, and methods for producing useful substances, e.g., acetaldehyde and ethanol, using such host cells.
Full Text Available February 2008
Type II restriction-modification system methylation subunit of Alicyclobacillus acidocaldarius

Patent Lee, Brady D ; Newby, Deborah T ; Lacey, Jeffrey A ; Thompson, David N ; Thompson, Vicki S ; Apel, William A ; Roberto, Francisco F ; Reed, David W

Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for modulating or altering recombination inside or outside of a cell using isolated and/or purified polypeptides and/or nucleic acid sequences from Alicyclobacillus acidocaldarius.
Full Text Available October 2013
Nested methylation-specific polymerase chain reaction cancer detection method

Patent Belinsky, Steven A ; Palmisano, William A

A molecular marker-based method for monitoring and detecting cancer in humans. Aberrant methylation of gene promoters is a marker for cancer risk in humans. A two-stage, or "nested" polymerase chain reaction method is disclosed for detecting methylated DNA sequences at sufficiently high levels of sensitivity to permit cancer screening in biological fluid samples, such as sputum, obtained non-invasively. The method is for detecting the aberrant methylation of the p16 gene, O 6-methylguanine-DNA methyltransferase gene, Death-associated protein kinase gene, RAS-associated family 1 gene, or other gene promoters. The method offers a potentially powerful approach to population-based screening for the detection ofmore » lung and other cancers.« less
Full Text Available May 2007
Nested methylation-specific polymerase chain reaction cancer detection method

Patent Belinsky, Steven A ; Palmisano, William A

A molecular marker-based method for monitoring and detecting cancer in humans. Aberrant methylation of gene promoters is a marker for cancer risk in humans. A two-stage, or "nested" polymerase chain reaction method is disclosed for detecting methylated DNA sequences at sufficiently high levels of sensitivity to permit cancer screening in biological fluid samples, such as sputum, obtained non-invasively. The method is for detecting the aberrant methylation of the p16 gene, O 6-methylguanine-DNA methyltransferase gene, Death-associated protein kinase gene, RAS-associated family 1 gene, or other gene promoters. The method offers a potentially powerful approach to population-based screening for the detection ofmore » lung and other cancers.« less
Full Text Available May 2007
Type II restriction-modification system methylation subunit of Alicyclobacillus acidocaldarius

Patent Lee, Brady D ; Newby, Deborah T ; Lacey, Jeffrey A ; Thompson, David N ; Thompson, Vicki S ; Apel, William A ; Roberto, Francisco F ; Reed, David W

Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for modulating or altering recombination inside or outside of a cell using isolated and/or purified polypeptides and/or nucleic acid sequences from Alicyclobacillus acidocaldarius.
Full Text Available May 2015