DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Mercury methylation genes in bacteria and archaea

Abstract

Two genes required for this mercury methylation have been identified in bacteria and archaea. These genes are the hgcA gene and hgcB, a corrinoid protein that facilitates methyl group transfer to Hg, and a corrinoid protein-associated ferredoxin with two [4Fe-4S] binding motifs involved in generating cob(I)almin, respectively. The invention provides nucleic acid probes and primers for detecting methylmercury and or for assessing mercury methylation potential in environmental, clinical and other samples. The invention also provides antibodies against these proteins, antibodies against these proteins, methods of using the antibodies and methods of biocatalysis.

Inventors:
;
Issue Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1464626
Patent Number(s):
10036072
Application Number:
14/132,906
Assignee:
UT-BATTELLE, LLC (Oak Ridge, TN)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 Dec 18
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Parks, Jerry M., and Johs, Alexander. Mercury methylation genes in bacteria and archaea. United States: N. p., 2018. Web.
Parks, Jerry M., & Johs, Alexander. Mercury methylation genes in bacteria and archaea. United States.
Parks, Jerry M., and Johs, Alexander. Tue . "Mercury methylation genes in bacteria and archaea". United States. https://www.osti.gov/servlets/purl/1464626.
@article{osti_1464626,
title = {Mercury methylation genes in bacteria and archaea},
author = {Parks, Jerry M. and Johs, Alexander},
abstractNote = {Two genes required for this mercury methylation have been identified in bacteria and archaea. These genes are the hgcA gene and hgcB, a corrinoid protein that facilitates methyl group transfer to Hg, and a corrinoid protein-associated ferredoxin with two [4Fe-4S] binding motifs involved in generating cob(I)almin, respectively. The invention provides nucleic acid probes and primers for detecting methylmercury and or for assessing mercury methylation potential in environmental, clinical and other samples. The invention also provides antibodies against these proteins, antibodies against these proteins, methods of using the antibodies and methods of biocatalysis.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {7}
}

Works referenced in this record:

Structural Basis for Electron and Methyl-Group Transfer in a Methyltransferase System Operating in the Reductive Acetyl-CoA Pathway
journal, August 2011


Draft Genome Sequences for Three Mercury-Methylating, Sulfate-Reducing Bacteria
journal, June 2013


Visualizing molecular juggling within a B12-dependent methyltransferase complex
journal, March 2012


Genome Sequence of the Mercury-Methylating Strain Desulfovibrio desulfuricans ND132
journal, February 2011


Acetogenesis and the Wood–Ljungdahl pathway of CO2 fixation
journal, December 2008


The Many Faces of Vitamin B12: Catalysis by Cobalamin-Dependent Enzymes
journal, June 2003


Structural insights into methyltransfer reactions of a corrinoid iron-sulfur protein involved in acetyl-CoA synthesis
journal, September 2006


Development of a Markerless Genetic Exchange System for Desulfovibrio vulgaris Hildenborough and Its Use in Generating a Strain with Increased Transformation Efficiency
journal, October 2009


Metabolic Pathways Leading to Mercury Methylation in Desulfovibrio desulfuricans LS †
journal, January 1994


Contribution of Coexisting Sulfate and Iron Reducing Bacteria to Methylmercury Production in Freshwater River Sediments
journal, February 2012


Sulfate-Reducing Bacteria: Principal Methylators of Mercury in Anoxic Estuarine Sediment †
journal, January 1985


Noncoding RNA genes identified in AT-rich hyperthermophiles
journal, May 2002


Synthesis of Methyl-mercury Compounds by Extracts of a Methanogenic Bacterium
journal, October 1968


The Genetic Basis for Bacterial Mercury Methylation
journal, February 2013


Sulfate-Reducing Bacteria Methylate Mercury at Variable Rates in Pure Culture and in Marine Sediments
journal, June 2000


Simultaneous determination of mercury methylation and demethylation capacities of various sulfate-reducing bacteria using species-specific isotopic tracers
journal, December 2010

  • Bridou, Romain; Monperrus, Mathilde; Gonzalez, Pablo Rodriguez
  • Environmental Toxicology and Chemistry, Vol. 30, Issue 2, p. 337-344
  • https://doi.org/10.1002/etc.395

Sulfate-Reducing Bacterium Desulfovibrio desulfuricans ND132 as a Model for Understanding Bacterial Mercury Methylation
journal, April 2011


Kinetics of substitution of co-ordinated carbanions in cobalt(III) corrinoids
journal, January 1970


Enzymatic catalysis of mercury methylation by Desulfovibrio desulfuricans LS.
journal, January 1994


Catalysis of Methyl Group Transfers Involving Tetrahydrofolate and B12
book, January 2008


The First X-ray Structural Evidence Demonstrating Thiolate Coordination in an Organocobalt B12 Model Complex:  Implications for Methionine Synthase
journal, January 1997


Mercury Methylation Independent of the Acetyl-Coenzyme A Pathway in Sulfate-Reducing Bacteria
journal, September 2003


Methods for Engineering Sulfate Reducing Bacteria of the Genus Desulfovibrio
book, January 2011


Detailed Assessment of the Kinetics of Hg-Cell Association, Hg Methylation, and Methylmercury Degradation in Several Desulfovibrio Species
journal, August 2012


Mercury Methylation by Dissimilatory Iron-Reducing Bacteria
journal, October 2006


Sequencing Intractable DNA to Close Microbial Genomes
journal, July 2012


Cobalt Limitation of Growth and Mercury Methylation in Sulfate-Reducing Bacteria
journal, January 2008