Mercury methylation genes in bacteria and archaea
Abstract
Two genes required for this mercury methylation have been identified in bacteria and archaea. These genes are the hgcA gene and hgcB, a corrinoid protein that facilitates methyl group transfer to Hg, and a corrinoid protein-associated ferredoxin with two [4Fe-4S] binding motifs involved in generating cob(I)almin, respectively. The invention provides nucleic acid probes and primers for detecting methylmercury and or for assessing mercury methylation potential in environmental, clinical and other samples. The invention also provides antibodies against these proteins, antibodies against these proteins, methods of using the antibodies and methods of biocatalysis.
- Inventors:
- Issue Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1464626
- Patent Number(s):
- 10,036,072
- Application Number:
- 14/132,906
- Assignee:
- UT-BATTELLE, LLC (Oak Ridge, TN)
- DOE Contract Number:
- AC05-00OR22725
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2013 Dec 18
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Parks, Jerry M., and Johs, Alexander. Mercury methylation genes in bacteria and archaea. United States: N. p., 2018.
Web.
Parks, Jerry M., & Johs, Alexander. Mercury methylation genes in bacteria and archaea. United States.
Parks, Jerry M., and Johs, Alexander. Tue .
"Mercury methylation genes in bacteria and archaea". United States. https://www.osti.gov/servlets/purl/1464626.
@article{osti_1464626,
title = {Mercury methylation genes in bacteria and archaea},
author = {Parks, Jerry M. and Johs, Alexander},
abstractNote = {Two genes required for this mercury methylation have been identified in bacteria and archaea. These genes are the hgcA gene and hgcB, a corrinoid protein that facilitates methyl group transfer to Hg, and a corrinoid protein-associated ferredoxin with two [4Fe-4S] binding motifs involved in generating cob(I)almin, respectively. The invention provides nucleic acid probes and primers for detecting methylmercury and or for assessing mercury methylation potential in environmental, clinical and other samples. The invention also provides antibodies against these proteins, antibodies against these proteins, methods of using the antibodies and methods of biocatalysis.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {7}
}
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Works referenced in this record:
Noncoding RNA genes identified in AT-rich hyperthermophiles
journal, May 2002
- Klein, R. J.; Misulovin, Z.; Eddy, S. R.
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 11, p. 7542-7547
The Many Faces of Vitamin B12: Catalysis by Cobalamin-Dependent Enzymes
journal, June 2003
- Banerjee, Ruma; Ragsdale, Stephen W.
- Annual Review of Biochemistry, Vol. 72, Issue 1, p. 209-247
Simultaneous determination of mercury methylation and demethylation capacities of various sulfate-reducing bacteria using species-specific isotopic tracers
journal, December 2010
- Bridou, Romain; Monperrus, Mathilde; Gonzalez, Pablo Rodriguez
- Environmental Toxicology and Chemistry, Vol. 30, Issue 2, p. 337-344
Mercury Methylation Independent of the Acetyl-Coenzyme A Pathway in Sulfate-Reducing Bacteria
journal, September 2003
- Ekstrom, E. B.; Morel, F. M. M.; Benoit, J. M.
- Applied and Environmental Microbiology, Vol. 69, Issue 9, p. 5414-5422
Structural Basis for Electron and Methyl-Group Transfer in a Methyltransferase System Operating in the Reductive Acetyl-CoA Pathway
journal, August 2011
- Goetzl, Sebastian; Jeoung, Jae-Hun; Hennig, Sandra Elisabeth
- Journal of Molecular Biology, Vol. 411, Issue 1, p. 96-109
Detailed Assessment of the Kinetics of Hg-Cell Association, Hg Methylation, and Methylmercury Degradation in Several Desulfovibrio Species
journal, August 2012
- Graham, Andrew M.; Bullock, Allyson L.; Maizel, Andrew C.
- Applied and Environmental Microbiology, Vol. 78, Issue 20, p. 7337-7346
Development of a Markerless Genetic Exchange System for Desulfovibrio vulgaris Hildenborough and Its Use in Generating a Strain with Increased Transformation Efficiency
journal, October 2009
- Keller, K. L.; Bender, K. S.; Wall, J. D.
- Applied and Environmental Microbiology, Vol. 75, Issue 24, p. 7682-7691
Methods for Engineering Sulfate Reducing Bacteria of the Genus Desulfovibrio
book, January 2011
- Keller, Kimberly L.; Wall, Judy D.; Chhabra, Swapnil
- Synthetic Biology, Part A, p. 503-517
Mercury Methylation by Dissimilatory Iron-Reducing Bacteria
journal, October 2006
- Kerin, E. J.; Gilmour, C. C.; Roden, E.
- Applied and Environmental Microbiology, Vol. 72, Issue 12, p. 7919-7921
Sulfate-Reducing Bacteria Methylate Mercury at Variable Rates in Pure Culture and in Marine Sediments
journal, June 2000
- King, J. K.; Kostka, J. E.; Frischer, M. E.
- Applied and Environmental Microbiology, Vol. 66, Issue 6, p. 2430-2437
Visualizing molecular juggling within a B12-dependent methyltransferase complex
journal, March 2012
- Kung, Yan; Ando, Nozomi; Doukov, Tzanko I.
- Nature, Vol. 484, Issue 7393, p. 265-269
The First X-ray Structural Evidence Demonstrating Thiolate Coordination in an Organocobalt B12 Model Complex: Implications for Methionine Synthase
journal, January 1997
- Polson, Suzette M.; Hansen, Lory; Marzilli, Luigi G.
- Inorganic Chemistry, Vol. 36, Issue 3, p. 307-313
Overview of Mercury Methylation Capacities among Anaerobic Bacteria Including Representatives of the Sulphate-Reducers: Implications for Environmental Studies
journal, January 2009
- Ranchou-Peyruse, M.; Monperrus, M.; Bridou, R.
- Geomicrobiology Journal, Vol. 26, Issue 1, p. 1-8
Reductive dealkylation of alkylcobaloximes, alkylcobalamins, and related compounds: Simulation of corrin dependent reductase and methyl group transfer reactions
journal, January 1973
- Schrauzer, Gerhard N.; Seck, Jane A.; Holland, Robert J.
- Bioinorganic Chemistry, Vol. 2, Issue 2, p. 93-124
Synthesis of Methyl-mercury Compounds by Extracts of a Methanogenic Bacterium
journal, October 1968
- Wood, J. M.; Kennedy, F. Scott; Rosen, C. G.
- Nature, Vol. 220, Issue 5163, p. 173-174
Contribution of Coexisting Sulfate and Iron Reducing Bacteria to Methylmercury Production in Freshwater River Sediments
journal, February 2012
- Yu, Ri-Qing; Flanders, J. R.; Mack, E. Erin
- Environmental Science & Technology, Vol. 46, Issue 5, p. 2684-2691
Genome Sequence of the Mercury-Methylating Strain Desulfovibrio desulfuricans ND132
journal, February 2011
- Brown, S. D.; Gilmour, C. C.; Kucken, A. M.
- Journal of Bacteriology, Vol. 193, Issue 8, p. 2078-2079
Sulfate-Reducing Bacterium Desulfovibrio desulfuricans ND132 as a Model for Understanding Bacterial Mercury Methylation
journal, April 2011
- Gilmour, Cynthia C.; Elias, Dwayne A.; Kucken, Amy M.
- Applied and Environmental Microbiology, Vol. 77, Issue 12, p. 3938-3951
Acetogenesis and the Wood–Ljungdahl pathway of CO2 fixation
journal, December 2008
- Ragsdale, Stephen W.; Pierce, Elizabeth
- Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1784, Issue 12, p. 1873-1898
Catalysis of Methyl Group Transfers Involving Tetrahydrofolate and B12
book, January 2008
- Ragsdale, Stephen W.
- Folic Acid and Folates, p. 293-324
Structural insights into methyltransfer reactions of a corrinoid iron-sulfur protein involved in acetyl-CoA synthesis
journal, September 2006
- Svetlitchnaia, T.; Svetlitchnyi, V.; Meyer, O.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 39, p. 14331-14336
Sequencing Intractable DNA to Close Microbial Genomes
journal, July 2012
- Hurt, Richard A.; Brown, Steven D.; Podar, Mircea
- PLoS ONE, Vol. 7, Issue 7, Article No. e41295
The Genetic Basis for Bacterial Mercury Methylation
journal, February 2013
- Parks, J. M.; Johs, A.; Podar, M.
- Science, Vol. 339, Issue 6125, p. 1332-1335
Cobalt Limitation of Growth and Mercury Methylation in Sulfate-Reducing Bacteria
journal, January 2008
- Ekstrom, Eileen B.; Morel, François M. M.
- Environmental Science & Technology, Vol. 42, Issue 1, p. 93-99
Draft Genome Sequences for Three Mercury-Methylating, Sulfate-Reducing Bacteria
journal, June 2013
- Brown, S. D.; Hurt, R. A.; Gilmour, C. C.
- Genome Announcements, Vol. 1, Issue 4, Article No. e00618-13