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Title: Detection of bioagents using a shear horizontal surface acoustic wave biosensor

Abstract

Viruses and other bioagents are of high medical and biodefense concern and their detection at concentrations well below the threshold necessary to cause health hazards continues to be a challenge with respect to sensitivity, specificity, and selectivity. Ideally, assays for accurate and real time detection of viral agents and other bioagents would not necessitate any pre-processing of the analyte, which would make them applicable for example to bodily fluids (blood, sputum) and man-made as well as naturally occurring bodies of water (pools, rivers). We describe herein a robust biosensor that combines the sensitivity of surface acoustic waves (SAW) generated at a frequency of 325 MHz with the specificity provided by antibodies and other ligands for the detection of viral agents. In preferred embodiments, a lithium tantalate based SAW transducer with silicon dioxide waveguide sensor platform featuring three test and one reference delay lines was used to adsorb antibodies directed against Coxsackie virus B4 or the negative-stranded category A bioagent Sin Nombre virus (SNV), a member of the genus Hantavirus, family Bunyaviridae, negative-stranded RNA viruses. Rapid detection (within seconds) of increasing concentrations of viral particles was linear over a range of order of magnitude for both viruses, although the sensor wasmore » approximately 50.times.10.sup.4-fold more sensitive for the detection of SNV. For both pathogens, the sensor's selectivity for its target was not compromised by the presence of confounding Herpes Simplex virus type 1. The biosensor was able to detect SNV at doses lower than the load of virus typically found in a human patient suffering from hantavirus cardiopulmonary syndrome (HCPS). Further, in a proof-of-principle real world application, the SAW biosensor was capable of selectively detecting SNV agents in complex solutions, such as naturally occurring bodies of water (river, sewage effluent) without analyte pre-processing.

Inventors:
; ; ; ; ; ; ; ;
Issue Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1464204
Patent Number(s):
10031135
Application Number:
14/172,429
Assignee:
STC.UNM (Albuquerque, NM); SANDIA CORPORATION (Albuquerque, NM)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Resource Relation:
Patent File Date: 2014 Feb 04
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION; 60 APPLIED LIFE SCIENCES

Citation Formats

Larson, Richard S., Hjelle, Brian, Hall, Pam R., Brown, David C., Bisoffi, Marco, Brozik, Susan M., Branch, Darren W., Edwards, Thayne L., and Wheeler, David. Detection of bioagents using a shear horizontal surface acoustic wave biosensor. United States: N. p., 2018. Web.
Larson, Richard S., Hjelle, Brian, Hall, Pam R., Brown, David C., Bisoffi, Marco, Brozik, Susan M., Branch, Darren W., Edwards, Thayne L., & Wheeler, David. Detection of bioagents using a shear horizontal surface acoustic wave biosensor. United States.
Larson, Richard S., Hjelle, Brian, Hall, Pam R., Brown, David C., Bisoffi, Marco, Brozik, Susan M., Branch, Darren W., Edwards, Thayne L., and Wheeler, David. Tue . "Detection of bioagents using a shear horizontal surface acoustic wave biosensor". United States. https://www.osti.gov/servlets/purl/1464204.
@article{osti_1464204,
title = {Detection of bioagents using a shear horizontal surface acoustic wave biosensor},
author = {Larson, Richard S. and Hjelle, Brian and Hall, Pam R. and Brown, David C. and Bisoffi, Marco and Brozik, Susan M. and Branch, Darren W. and Edwards, Thayne L. and Wheeler, David},
abstractNote = {Viruses and other bioagents are of high medical and biodefense concern and their detection at concentrations well below the threshold necessary to cause health hazards continues to be a challenge with respect to sensitivity, specificity, and selectivity. Ideally, assays for accurate and real time detection of viral agents and other bioagents would not necessitate any pre-processing of the analyte, which would make them applicable for example to bodily fluids (blood, sputum) and man-made as well as naturally occurring bodies of water (pools, rivers). We describe herein a robust biosensor that combines the sensitivity of surface acoustic waves (SAW) generated at a frequency of 325 MHz with the specificity provided by antibodies and other ligands for the detection of viral agents. In preferred embodiments, a lithium tantalate based SAW transducer with silicon dioxide waveguide sensor platform featuring three test and one reference delay lines was used to adsorb antibodies directed against Coxsackie virus B4 or the negative-stranded category A bioagent Sin Nombre virus (SNV), a member of the genus Hantavirus, family Bunyaviridae, negative-stranded RNA viruses. Rapid detection (within seconds) of increasing concentrations of viral particles was linear over a range of order of magnitude for both viruses, although the sensor was approximately 50.times.10.sup.4-fold more sensitive for the detection of SNV. For both pathogens, the sensor's selectivity for its target was not compromised by the presence of confounding Herpes Simplex virus type 1. The biosensor was able to detect SNV at doses lower than the load of virus typically found in a human patient suffering from hantavirus cardiopulmonary syndrome (HCPS). Further, in a proof-of-principle real world application, the SAW biosensor was capable of selectively detecting SNV agents in complex solutions, such as naturally occurring bodies of water (river, sewage effluent) without analyte pre-processing.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {7}
}

Works referenced in this record:

System with Extended Range of Molecular Sensing Through Integrated Multi-modal Data Acquisition
patent-application, August 2008


Detection of viral bioagents using a shear horizontal surface acoustic wave biosensor
journal, April 2008


Chemical Vapor Deposition of Conformal, Functional, and Responsive Polymer Films
journal, December 2009


A Love wave immunosensor for whole E. coli bacteria detection using an innovative two-step immobilisation approach
journal, April 2007


Diagnosis and treatment of new world hantavirus infections
journal, January 2006


Acoustic wave sensor apparatus, method and system using wide bandgap materials
patent-application, October 2003


Surface acoustic wave sensors and method for detecting target analytes
patent-application, April 2004


Real time device for biosensing: design of a bacteriophage model using love acoustic waves
journal, May 2003


Pulse mode shear horizontal-surface acoustic wave (SH-SAW) system for liquid based sensing applications
journal, January 2004


Detection Systems and Methods
patent-application, June 2008


Enteroviruses as agents of emerging infectious diseases
journal, January 2005


Microarray-based method for rapid identification of cells, microorganisms, or protein mixtures
patent-application, December 2002


Differentiation and identification of analogous chemical or biological substances with biosensors
patent-application, September 2006


Guided Shear Horizontal Surface Acoustic Wave Sensors for Chemical and Biochemical Detection in Liquids
journal, December 2001


Rapid Detection of Human Immunodeficiency Virus Types 1 and 2 by Use of an Improved Piezoelectric Biosensor
journal, March 2013


Assay method for biological target complexes on the surface of a biosensor
patent, August 1997


Biosensors for the detection of bacteria
journal, February 2004


Sin Nombre Viral RNA Load in Patients with Hantavirus Cardiopulmonary Syndrome
journal, November 2006


Design and properties of quartz-based Love wave acoustic sensors incorporating silicon dioxide and PMMA guiding layers
journal, December 1997


Systems and methods for molecular recognition
patent-application, February 2006


Suppression of transverse-mode spurious responses for saw resonators on SiO2/Al/LiNbO3 structure by selective removal of SiO2
journal, October 2011


Seroepidemiologic Studies of Hantavirus Infection Among Wild Rodents in California
journal, June 1997


Detection of bioagents using a shear horizontal surface acoustic wave biosensor
patent, April 2014


Selection and characterization of scFv antibodies against the Sin Nombre hantavirus nucleocapsid protein
journal, April 2007


Ambipolar organic transistor
patent-application, May 2003


SAW-grade SiO2 for advanced microfluidic devices
conference, May 2009


Viral Agents as Biological Weapons and Agents of Bioterrorism
journal, June 2002


Detection of Escherichia coli O157:H7 with langasite pure shear horizontal surface acoustic wave sensors
journal, June 2006


Vaccines against hantaviruses
journal, October 2002


4D-4 Love Wave Acoustic Array Biosensor Platform for Autonomous Detection
conference, October 2007