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Title: Methods for generating barcoded combinatorial libraries

Abstract

Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
Inscripta, Inc., Boulder, CO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1463088
Patent Number(s):
10,017,760
Application Number:
15/632,222
Assignee:
Inscripta, Inc. (Boulder, CO); The Regents of the University of Colorado, a Body Corporate (Denver, CO)
DOE Contract Number:  
SC0008812
Resource Type:
Patent
Resource Relation:
Patent File Date: 2017 Jun 23
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Gill, Ryan T., Garst, Andrew, Lipscomb, Tanya Elizabeth Warnecke, Bassalo, Marcelo Colika, and Zeitoun, Ramsey Ibrahim. Methods for generating barcoded combinatorial libraries. United States: N. p., 2018. Web.
Gill, Ryan T., Garst, Andrew, Lipscomb, Tanya Elizabeth Warnecke, Bassalo, Marcelo Colika, & Zeitoun, Ramsey Ibrahim. Methods for generating barcoded combinatorial libraries. United States.
Gill, Ryan T., Garst, Andrew, Lipscomb, Tanya Elizabeth Warnecke, Bassalo, Marcelo Colika, and Zeitoun, Ramsey Ibrahim. Tue . "Methods for generating barcoded combinatorial libraries". United States. https://www.osti.gov/servlets/purl/1463088.
@article{osti_1463088,
title = {Methods for generating barcoded combinatorial libraries},
author = {Gill, Ryan T. and Garst, Andrew and Lipscomb, Tanya Elizabeth Warnecke and Bassalo, Marcelo Colika and Zeitoun, Ramsey Ibrahim},
abstractNote = {Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {7}
}

Patent:

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  • DOI: 10.1186/1754-6834-6-57

Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination
journal, September 2013

  • Dickinson, Daniel J.; Ward, Jordan D.; Reiner, David J.
  • Nature Methods, Vol. 10, Issue 10, p. 1028-1034
  • DOI: 10.1038/nmeth.2641