Methods for generating barcoded combinatorial libraries
Abstract
Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.
- Inventors:
- Issue Date:
- Research Org.:
- Inscripta, Inc., Boulder, CO (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1463088
- Patent Number(s):
- 10017760
- Application Number:
- 15/632,222
- Assignee:
- Inscripta, Inc. (Boulder, CO); The Regents of the University of Colorado, a Body Corporate (Denver, CO)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- SC0008812
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2017 Jun 23
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Gill, Ryan T., Garst, Andrew, Lipscomb, Tanya Elizabeth Warnecke, Bassalo, Marcelo Colika, and Zeitoun, Ramsey Ibrahim. Methods for generating barcoded combinatorial libraries. United States: N. p., 2018.
Web.
Gill, Ryan T., Garst, Andrew, Lipscomb, Tanya Elizabeth Warnecke, Bassalo, Marcelo Colika, & Zeitoun, Ramsey Ibrahim. Methods for generating barcoded combinatorial libraries. United States.
Gill, Ryan T., Garst, Andrew, Lipscomb, Tanya Elizabeth Warnecke, Bassalo, Marcelo Colika, and Zeitoun, Ramsey Ibrahim. Tue .
"Methods for generating barcoded combinatorial libraries". United States. https://www.osti.gov/servlets/purl/1463088.
@article{osti_1463088,
title = {Methods for generating barcoded combinatorial libraries},
author = {Gill, Ryan T. and Garst, Andrew and Lipscomb, Tanya Elizabeth Warnecke and Bassalo, Marcelo Colika and Zeitoun, Ramsey Ibrahim},
abstractNote = {Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jul 10 00:00:00 EDT 2018},
month = {Tue Jul 10 00:00:00 EDT 2018}
}
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