TNT cloning system
Abstract
Disclosed herein are vectors and components for a nucleic acid cloning system, and methods of use of the vectors and components in cloning nucleic acid fragments of interest. The cloning system includes two families of destination vectors which can be used in alternating form to systematically combine nucleic acid fragments of interest.
- Inventors:
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1463001
- Patent Number(s):
- 10017770
- Application Number:
- 14/789,112
- Assignee:
- UT-BATTELLE, LLC (Oak Ridge, TN)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC05-000R22725
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2015 Jul 01
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Tuskan, Gerald A., Yang, Xiaohan, and Cestari De Paoli, Henrique. TNT cloning system. United States: N. p., 2018.
Web.
Tuskan, Gerald A., Yang, Xiaohan, & Cestari De Paoli, Henrique. TNT cloning system. United States.
Tuskan, Gerald A., Yang, Xiaohan, and Cestari De Paoli, Henrique. Tue .
"TNT cloning system". United States. https://www.osti.gov/servlets/purl/1463001.
@article{osti_1463001,
title = {TNT cloning system},
author = {Tuskan, Gerald A. and Yang, Xiaohan and Cestari De Paoli, Henrique},
abstractNote = {Disclosed herein are vectors and components for a nucleic acid cloning system, and methods of use of the vectors and components in cloning nucleic acid fragments of interest. The cloning system includes two families of destination vectors which can be used in alternating form to systematically combine nucleic acid fragments of interest.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {7}
}
Works referenced in this record:
Nucleocytoplasmic Distribution Is Required for Activation of Resistance by the Potato NB-LRR Receptor Rx1 and Is Balanced by Its Functional Domains
journal, December 2010
- Slootweg, Erik; Roosien, Jan; Spiridon, Laurentiu N.
- The Plant Cell, Vol. 22, Issue 12, p. 4195-4215
REBASE—a database for DNA restriction and modification: enzymes, genes and genomes
journal, November 2014
- Roberts, Richard J.; Vincze, Tamas; Posfai, Janos
- Nucleic Acids Research, Vol. 43, Issue D1, p. D298-D299
The ‘cleavage’ activities of foot-and-mouth disease virus 2A site-directed mutants and naturally occurring ‘2A-like’ sequences
journal, May 2001
- ten Dam, Edwin; Donnelly, Michelle L. L.; Gani, David
- Journal of General Virology, Vol. 82, Issue 5
GoldenBraid: An Iterative Cloning System for Standardized Assembly of Reusable Genetic Modules
journal, July 2011
- Sarrion-Perdigones, Alejandro; Falconi, Erica Elvira; Zandalinas, Sara I.
- PLoS ONE, Vol. 6, Issue 7, Article No. e21622
Fast, easy and efficient: site-specific insertion of transgenes into Enterobacterial chromosomes using Tn7 without need for selection of the insertion event
journal, January 2006
- McKenzie, Gregory J.; Craig, Nancy L.
- BMC Microbiology, Vol. 6, Issue 1, Article No. 39
GoldenBraid 2.0: A Comprehensive DNA Assembly Framework for Plant Synthetic Biology
journal, May 2013
- Sarrion-Perdigones, A.; Vazquez-Vilar, M.; Palaci, J.
- Plant Physiology, Vol. 162, Issue 3, p. 1618-1631
j5 DNA Assembly Design Automation Software
journal, December 2011
- Hillson, Nathan J.; Rosengarten, Rafael D.; Keasling, Jay D.
- ACS Synthetic Biology, Vol. 1, Issue 1, p. 14-21
Arabidopsis Tetraspanins Are Confined to Discrete Expression Domains and Cell Types in Reproductive Tissues and Form Homo- and Heterodimers When Expressed in Yeast
journal, August 2013
- Boavida, L. C.; Qin, P.; Broz, M.
- Plant Physiology, Vol. 163, Issue 2, p. 696-712
Endosomolytic polymers
patent-application, October 2004
- Rozem, David B.; Wakefield, Darren; Wolff, Jon A.
- US Patent Application 10/816081; 20040198687
Iterative plug-and-play methodology for constructing and modifying synthetic gene networks
journal, October 2012
- Litcofsky, Kevin D.; Afeyan, Raffi B.; Krom, Russell J.
- Nature Methods, Vol. 9, Issue 11, p. 1077-1080
Characterizing the Protonation State of Cytosine in Transient G·C Hoogsteen Base Pairs in Duplex DNA
journal, April 2013
- Nikolova, Evgenia N.; Goh, Garrett B.; Brooks, Charles L.
- Journal of the American Chemical Society, Vol. 135, Issue 18, p. 6766-6769
Intercalator conjugates of pyrimidine locked nucleic acid-modified triplex-forming oligonucleotides: improving DNA binding properties and reaching cellular activities
journal, July 2005
- Brunet, Erika; Corgnali, Maddalena; Perrouault, Loïc
- Nucleic Acids Research, Vol. 33, Issue 13, p. 4223-4234
Methods, compositions and kits for a one-step DNA cloning system
patent, December 2015
- Minshull, Jeremy; Ness, Jon E.; Theodorou, Elias
- US Patent Document 9,206,433
Enzymatic assembly of DNA molecules up to several hundred kilobases
journal, April 2009
- Gibson, Daniel G.; Young, Lei; Chuang, Ray-Yuan
- Nature Methods, Vol. 6, Issue 5, p. 343-345
Synthetic biology as it relates to CAM photosynthesis: challenges and opportunities
journal, February 2014
- DePaoli, Henrique C.; Borland, Anne M.; Tuskan, Gerald A.
- Journal of Experimental Botany, Vol. 65, Issue 13, p. 3381-3393
A One Pot, One Step, Precision Cloning Method with High Throughput Capability
journal, November 2008
- Engler, Carola; Kandzia, Romy; Marillonnet, Sylvestre
- PLoS ONE, Vol. 3, Issue 11, Article No. e3647
Type IIS Restriction Enzyme Footprinting I. Measurement of a Triple Helix Dissociation Constant With Eco57I at 25°C
journal, June 1996
- Ward, B.
- Nucleic Acids Research, Vol. 24, Issue 12, p. 2435-2440
Protein Interaction Mapping in C. elegans Using Proteins Involved in Vulval Development
journal, January 2000
- Walhout, Albertha J. M.; Sordella, Raffaella; Lu, Xiaowei
- Science, Vol. 287, Issue 5450, p. 116-122
High Cleavage Efficiency of a 2A Peptide Derived from Porcine Teschovirus-1 in Human Cell Lines, Zebrafish and Mice
journal, April 2011
- Kim, Jin Hee; Lee, Sang-Rok; Li, Li-Hua
- PLoS ONE, Vol. 6, Issue 4, Article No. e18556
Transgenic Expression in Arabidopsis of a Polyprotein Construct Leading to Production of Two Different Antimicrobial Proteins
journal, April 2002
- François, Isabelle E. J. A.; De Bolle, Miguel F. C.; Dwyer, Geoff
- Plant Physiology, Vol. 128, Issue 4, p. 1346-1358
The small, versatilepPZP family ofAgrobacterium binary vectors for plant transformation
journal, September 1994
- Hajdukiewicz, Peter; Svab, Zora; Maliga, Pal
- Plant Molecular Biology, Vol. 25, Issue 6, p. 989-994
Rapid, modular and reliable construction of complex mammalian gene circuits
journal, July 2013
- Guye, Patrick; Li, Yinqing; Wroblewska, Liliana
- Nucleic Acids Research, Vol. 41, Issue 16, p. e156-e156
Method for the preparation of a nucleic acid library
patent, August 2015
- Van Den Brulle, Jan; Fuhrmann, Markus; Strohner, Ralf
- US Patent Document 9,115,352