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Title: Metabolic evolution of Escherichia coli strains that produce organic acids

Abstract

This invention relates to the metabolic evolution of a microbial organism previously optimized for producing an organic acid in commercially significant quantities under fermentative conditions using a hexose sugar as sole source of carbon in a minimal mineral medium. As a result of this metabolic evolution, the microbial organism acquires the ability to use pentose sugars derived from cellulosic materials for its growth while retaining the original growth kinetics, the rate of organic acid production and the ability to use hexose sugars as a source of carbon. This invention also discloses the genetic change in the microorganism that confers the ability to use both the hexose and pentose sugars simultaneously in the production of commercially significant quantities of organic acids.

Inventors:
; ;
Issue Date:
Research Org.:
Myriant Corporation, Woburn, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1462996
Patent Number(s):
10017793
Application Number:
14/491,073
Assignee:
Myriant Corporation (Woburn, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
EE0002878
Resource Type:
Patent
Resource Relation:
Patent File Date: 2014 Sep 19
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Grabar, Tammy, Gong, Wei, and Yocum, R. Rogers. Metabolic evolution of Escherichia coli strains that produce organic acids. United States: N. p., 2018. Web.
Grabar, Tammy, Gong, Wei, & Yocum, R. Rogers. Metabolic evolution of Escherichia coli strains that produce organic acids. United States.
Grabar, Tammy, Gong, Wei, and Yocum, R. Rogers. Tue . "Metabolic evolution of Escherichia coli strains that produce organic acids". United States. https://www.osti.gov/servlets/purl/1462996.
@article{osti_1462996,
title = {Metabolic evolution of Escherichia coli strains that produce organic acids},
author = {Grabar, Tammy and Gong, Wei and Yocum, R. Rogers},
abstractNote = {This invention relates to the metabolic evolution of a microbial organism previously optimized for producing an organic acid in commercially significant quantities under fermentative conditions using a hexose sugar as sole source of carbon in a minimal mineral medium. As a result of this metabolic evolution, the microbial organism acquires the ability to use pentose sugars derived from cellulosic materials for its growth while retaining the original growth kinetics, the rate of organic acid production and the ability to use hexose sugars as a source of carbon. This invention also discloses the genetic change in the microorganism that confers the ability to use both the hexose and pentose sugars simultaneously in the production of commercially significant quantities of organic acids.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {7}
}

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Works referenced in this record:

Simultaneous utilization of d-cellobiose, d-glucose, and d-xylose by recombinant Corynebacterium glutamicum under oxygen-deprived conditions
journal, September 2008


Identification by Comprehensive Chimeric Analysis of a Key Residue Responsible for High Affinity Glucose Transport by Yeast HXT2
journal, March 2007


Construction of an Escherichia coli K-12 Mutant for Homoethanologenic Fermentation of Glucose or Xylose without Foreign Genes
journal, January 2007


Mutants of Escherichia coli producing pyrroloquinoline quinone
journal, August 1991


Improved strains of recombinant Escherichia coli for ethanol production from sugar mixtures
journal, April 1995


Ins and Outs of Major Facilitator Superfamily Antiporters
journal, October 2008


Characterization, Expression, and Mutation of the Lactococcus lactis galPMKTE Genes, Involved in Galactose Utilization via the Leloir Pathway
journal, February 2003


Solid-State NMR Spectroscopy Detects Interactions between Tryptophan Residues of the E. coli Sugar Transporter GalP and the α-Anomer of the d-Glucose Substrate
journal, January 2008

  • Patching, Simon G.; Henderson, Peter J. F.; Herbert, Richard B.
  • Journal of the American Chemical Society, Vol. 130, Issue 4, p. 1236-1244
  • https://doi.org/10.1021/ja075584k

Characterization of Constitutive Galactose Permease Mutants in Salmonella typhimurium
journal, January 1973


The mechanisms of carbon catabolite repression in bacteria
journal, April 2008


Combining metabolic engineering and metabolic evolution to develop nonrecombinant strains of Escherichia coli C that produce succinate and malate
journal, January 2008


Repression of galP, the galactose transporter in Escherichia coli, requires the specific regulator of N-acetylglucosamine metabolism
journal, January 2009


Characterization of sugar mixtures utilization by an Escherichia coli mutant devoid of the phosphotransferase system
journal, October 2001


Eight Amino Acid Residues in Transmembrane Segments of Yeast Glucose Transporter Hxt2 Are Required for High Affinity Transport
journal, April 2006


Analysis of NADPH supply during xylitol production by engineered Escherichia coli
journal, January 2009


Characterization of the individual glucose uptake systems of Lactococcus lactis: mannose-PTS, cellobiose-PTS and the novel GlcU permease
journal, February 2009


Two glucose/xylose transporter genes from the yeast Candida intermedia: first molecular characterization of a yeast xylose–H+ symporter
journal, May 2006


Engineering Escherichia coli for efficient conversion of glucose to pyruvate
journal, February 2004


Carbon catabolite repression in bacteria: many ways to make the most out of nutrients
journal, August 2008


Comprehensive Chimeric Analysis of Amino Acid Residues Critical for High Affinity Glucose Transport by Hxt2 of Saccharomyces cerevisiae
journal, May 2004


Transmembrane segments 1, 5, 7 and 8 are required for high-affinity glucose transport by Saccharomyces cerevisiae Hxt2 transporter
journal, May 2003


Expression of the Gxf1 transporter from Candida intermedia improves fermentation performance in recombinant xylose-utilizing Saccharomyces cerevisiae
journal, November 2008


Low salt medium for lactate and ethanol production by recombinant Escherichia coli B
journal, December 2006


How Phosphotransferase System-Related Protein Phosphorylation Regulates Carbohydrate Metabolism in Bacteria
journal, December 2006


Altered Glucose Transport and Shikimate Pathway Product Yields in E. coli
journal, October 2003


Bacterial PEP-Dependent Carbohydrate: Phosphotransferase Systems Couple Sensing and Global Control Mechanisms
book, January 2009


Engineering Escherichia coli for xylitol production from glucose-xylose mixtures
journal, December 2006


Evolutionary engineering of mixed-sugar utilization by a xylose-fermenting strain
journal, July 2005


Minimal Escherichia coli Cell for the Most Efficient Production of Ethanol from Hexoses and Pentoses
journal, April 2008


An in vivo , label-free quick assay for xylose transport in Escherichia coli
journal, July 2009


Asparagine 394 in Putative Helix 11 of the Galactose-H+ Symport Protein (GalP) from Escherichia coli Is Associated with the Internal Binding Site for Cytochalasin B and Sugar
journal, June 1997


Prefermentation improves xylose utilization in simultaneous saccharification and co-fermentation of pretreated spruce
journal, January 2009


Transport of xylose and glucose in the xylose-fermenting yeast Pichia stipitis
journal, February 1988


Eliminating side products and increasing succinate yields in engineered strains of Escherichia coli C
journal, December 2008


Genetically Engineered SaccharomycesYeast Capable of Effective Cofermentation of Glucose and Xylose
journal, May 1998


Engineering Transport Protein Function: Theoretical and Technical Considerations Using the Sugar-Transporting Phosphotransferase System of Escherichia coli as a Model System
journal, January 2006


Metabolic evolution of energy-conserving pathways for succinate production in Escherichia coli
journal, November 2009


Relative substrate affinities of wild-type and mutant forms of the Escherichia coli sugar transporter GalP determined by solid-state NMR
journal, January 2008


Major Facilitator Superfamily
journal, January 1998


Role of xylose transporters in xylitol production from engineered Escherichia coli
journal, April 2008


Proton-linked sugar transport systems in bacteria
journal, August 1990


Improved strains of recombinant Escherichia coli for ethanol production from sugar mixtures
journal, April 1995


Transport of galactose, glucose and their molecular analogues by Escherichia coli K12
journal, February 1977


Use of catabolite repression mutants for fermentation of sugar mixtures to ethanol
journal, July 2001


Xylose transport studies with xylose-utilizing Saccharomyces cerevisiae strains expressing heterologous and homologous permeases
journal, December 2006


Fermentation of sugar mixtures using Escherichia coli catabolite repression mutants engineered for production of L-lactic acid
journal, November 2002


Specific spin labelling of the sugar-H+ symporter, GalP, in cell membranes of Escherichia coli: site mobility and overall rotational diffusion of the protein
journal, February 2001


Hexose and pentose transport in ascomycetous yeasts: an overview
journal, June 2009