Method and apparatus for measuring biofilm thickness and topology

Title: Method and apparatus for measuring biofilm thickness and topology

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An apparatus and method of measuring biofilm and biological activity on a surface is disclosed. The apparatus includes a biofilm, which includes one or more microorganisms, grown on a substrate. A viewing window is placed on a surface of the biofilm and a gas bubble is introduced between the viewing window and the surface of the biofilm. The space between the substrate and the viewing window may be enclosed in a casing that has an inlet and an outlet, forming a flow cell. A microscope system, such as a white light interferometer, captures data of the biofilm in situ and non-destructively. The 3D images of biofilm surface have high resolution while maintaining a large field of view. The apparatuses and methods will be useful for fundamental studies of biofilms, biomedical and environmental screening, and many other applications in biology and the life sciences.

Inventors:: Larimer, Curtis J.; Suter, Jonathan D.; Addleman, Raymond S.; Bonheyo, George T.; Brann, Michelle R.

Issue Date:: 2018-06-26

OSTI Identifier:: 1460555

Assignee:: BATTELLE MEMORIAL INSTITUTE (Richland, WA) PNNL

Patent Number(s):: 10,005,999

Application Number:: 15/071,921

Contract Number:: AC0576RL01830

Resource Relation:: Patent File Date: 2016 Mar 16

Research Org:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Org:: USDOE

Country of Publication:: United States

Language:: English

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