Method and apparatus for measuring biofilm thickness and topology
Abstract
An apparatus and method of measuring biofilm and biological activity on a surface is disclosed. The apparatus includes a biofilm, which includes one or more microorganisms, grown on a substrate. A viewing window is placed on a surface of the biofilm and a gas bubble is introduced between the viewing window and the surface of the biofilm. The space between the substrate and the viewing window may be enclosed in a casing that has an inlet and an outlet, forming a flow cell. A microscope system, such as a white light interferometer, captures data of the biofilm in situ and non-destructively. The 3D images of biofilm surface have high resolution while maintaining a large field of view. The apparatuses and methods will be useful for fundamental studies of biofilms, biomedical and environmental screening, and many other applications in biology and the life sciences.
- Inventors:
- Issue Date:
- Research Org.:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1460555
- Patent Number(s):
- 10,005,999
- Application Number:
- 15/071,921
- Assignee:
- BATTELLE MEMORIAL INSTITUTE (Richland, WA) PNNL
- DOE Contract Number:
- AC0576RL01830
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2016 Mar 16
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Larimer, Curtis J., Suter, Jonathan D., Addleman, Raymond S., Bonheyo, George T., and Brann, Michelle R. Method and apparatus for measuring biofilm thickness and topology. United States: N. p., 2018.
Web.
Larimer, Curtis J., Suter, Jonathan D., Addleman, Raymond S., Bonheyo, George T., & Brann, Michelle R. Method and apparatus for measuring biofilm thickness and topology. United States.
Larimer, Curtis J., Suter, Jonathan D., Addleman, Raymond S., Bonheyo, George T., and Brann, Michelle R. Tue .
"Method and apparatus for measuring biofilm thickness and topology". United States. https://www.osti.gov/servlets/purl/1460555.
@article{osti_1460555,
title = {Method and apparatus for measuring biofilm thickness and topology},
author = {Larimer, Curtis J. and Suter, Jonathan D. and Addleman, Raymond S. and Bonheyo, George T. and Brann, Michelle R.},
abstractNote = {An apparatus and method of measuring biofilm and biological activity on a surface is disclosed. The apparatus includes a biofilm, which includes one or more microorganisms, grown on a substrate. A viewing window is placed on a surface of the biofilm and a gas bubble is introduced between the viewing window and the surface of the biofilm. The space between the substrate and the viewing window may be enclosed in a casing that has an inlet and an outlet, forming a flow cell. A microscope system, such as a white light interferometer, captures data of the biofilm in situ and non-destructively. The 3D images of biofilm surface have high resolution while maintaining a large field of view. The apparatuses and methods will be useful for fundamental studies of biofilms, biomedical and environmental screening, and many other applications in biology and the life sciences.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {6}
}