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Title: Method and apparatus for measuring biofilm thickness and topology

Abstract

An apparatus and method of measuring biofilm and biological activity on a surface is disclosed. The apparatus includes a biofilm, which includes one or more microorganisms, grown on a substrate. A viewing window is placed on a surface of the biofilm and a gas bubble is introduced between the viewing window and the surface of the biofilm. The space between the substrate and the viewing window may be enclosed in a casing that has an inlet and an outlet, forming a flow cell. A microscope system, such as a white light interferometer, captures data of the biofilm in situ and non-destructively. The 3D images of biofilm surface have high resolution while maintaining a large field of view. The apparatuses and methods will be useful for fundamental studies of biofilms, biomedical and environmental screening, and many other applications in biology and the life sciences.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1460555
Patent Number(s):
10005999
Application Number:
15/071,921
Assignee:
BATTELLE MEMORIAL INSTITUTE (Richland, WA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12M - APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
G - PHYSICS G01 - MEASURING G01B - MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS
DOE Contract Number:  
AC0576RL01830
Resource Type:
Patent
Resource Relation:
Patent File Date: 2016 Mar 16
Country of Publication:
United States
Language:
English

Citation Formats

Larimer, Curtis J., Suter, Jonathan D., Addleman, Raymond S., Bonheyo, George T., and Brann, Michelle R. Method and apparatus for measuring biofilm thickness and topology. United States: N. p., 2018. Web.
Larimer, Curtis J., Suter, Jonathan D., Addleman, Raymond S., Bonheyo, George T., & Brann, Michelle R. Method and apparatus for measuring biofilm thickness and topology. United States.
Larimer, Curtis J., Suter, Jonathan D., Addleman, Raymond S., Bonheyo, George T., and Brann, Michelle R. Tue . "Method and apparatus for measuring biofilm thickness and topology". United States. https://www.osti.gov/servlets/purl/1460555.
@article{osti_1460555,
title = {Method and apparatus for measuring biofilm thickness and topology},
author = {Larimer, Curtis J. and Suter, Jonathan D. and Addleman, Raymond S. and Bonheyo, George T. and Brann, Michelle R.},
abstractNote = {An apparatus and method of measuring biofilm and biological activity on a surface is disclosed. The apparatus includes a biofilm, which includes one or more microorganisms, grown on a substrate. A viewing window is placed on a surface of the biofilm and a gas bubble is introduced between the viewing window and the surface of the biofilm. The space between the substrate and the viewing window may be enclosed in a casing that has an inlet and an outlet, forming a flow cell. A microscope system, such as a white light interferometer, captures data of the biofilm in situ and non-destructively. The 3D images of biofilm surface have high resolution while maintaining a large field of view. The apparatuses and methods will be useful for fundamental studies of biofilms, biomedical and environmental screening, and many other applications in biology and the life sciences.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {6}
}

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Works referenced in this record:

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