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Title: Methods of expanding bacteriophage host-range and bacteriophage produced by the methods

A method of producing novel bacteriophages with expanded host-range and bacteriophages with expanded host ranges are disclosed. The method produces mutant phage strains which are infectious to a second host and can be more infectious to their natural host than in their natural state. The method includes repeatedly passaging a selected phage strain into bacterial cultures that contain varied ratios of its natural host bacterial strain with a bacterial strain that the phage of interest is unable to infect; the target-host. After each passage the resulting phage are purified and screened for activity against the target-host via double-overlay assays. When mutant phages that are shown to infect the target-host are discovered, they are further propagated in culture that contains only the target-host to produce a stock of the resulting mutant phage.
Inventors:
;
Issue Date:
OSTI Identifier:
1456914
Assignee:
National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM) SNL
Patent Number(s):
9,988,608
Application Number:
14/883,366
Contract Number:
AC04-94AL85000
Resource Relation:
Patent File Date: 2015 Oct 14
Research Org:
Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English