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Title: Methods of expanding bacteriophage host-range and bacteriophage produced by the methods

Abstract

A method of producing novel bacteriophages with expanded host-range and bacteriophages with expanded host ranges are disclosed. The method produces mutant phage strains which are infectious to a second host and can be more infectious to their natural host than in their natural state. The method includes repeatedly passaging a selected phage strain into bacterial cultures that contain varied ratios of its natural host bacterial strain with a bacterial strain that the phage of interest is unable to infect; the target-host. After each passage the resulting phage are purified and screened for activity against the target-host via double-overlay assays. When mutant phages that are shown to infect the target-host are discovered, they are further propagated in culture that contains only the target-host to produce a stock of the resulting mutant phage.

Inventors:
;
Issue Date:
Research Org.:
Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1456914
Patent Number(s):
9988608
Application Number:
14/883,366
Assignee:
National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015 Oct 14
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Crown, Kevin K., and Santarpia, Joshua. Methods of expanding bacteriophage host-range and bacteriophage produced by the methods. United States: N. p., 2018. Web.
Crown, Kevin K., & Santarpia, Joshua. Methods of expanding bacteriophage host-range and bacteriophage produced by the methods. United States.
Crown, Kevin K., and Santarpia, Joshua. Tue . "Methods of expanding bacteriophage host-range and bacteriophage produced by the methods". United States. https://www.osti.gov/servlets/purl/1456914.
@article{osti_1456914,
title = {Methods of expanding bacteriophage host-range and bacteriophage produced by the methods},
author = {Crown, Kevin K. and Santarpia, Joshua},
abstractNote = {A method of producing novel bacteriophages with expanded host-range and bacteriophages with expanded host ranges are disclosed. The method produces mutant phage strains which are infectious to a second host and can be more infectious to their natural host than in their natural state. The method includes repeatedly passaging a selected phage strain into bacterial cultures that contain varied ratios of its natural host bacterial strain with a bacterial strain that the phage of interest is unable to infect; the target-host. After each passage the resulting phage are purified and screened for activity against the target-host via double-overlay assays. When mutant phages that are shown to infect the target-host are discovered, they are further propagated in culture that contains only the target-host to produce a stock of the resulting mutant phage.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {6}
}

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Works referenced in this record:

Nucleoside Analogue Mutagenesis of a Single-Stranded DNA Virus: Evolution and Resistance
journal, June 2012


Old dogma, new tricks—21st Century phage therapy
journal, January 2004


Bacteriophage T4 Host Range is Expanded by Duplications of a Small Domain of the Tail Fiber Adhesin
journal, May 1996


Antibacterial agents
patent-application, September 2003


Alteration of tail fiber protein gp38 enables T2 phage to infect Escherichia coli O157:H7
journal, January 2005


Arbovirus evolution in vivo is constrained by host alternation
journal, May 2008


Genomic Sequence of Bacteriophage ATCC 8074-B1 and Activity of Its Endolysin and Engineered Variants against Clostridium sporogenes
journal, March 2012


Genome plasticity in the distal tail fiber locus of the T-even bacteriophage: recombination between conserved motifs swaps adhesin specificity
journal, September 1998


Numerical Taxonomy of Clostridium botulinum and Clostridium sporogenes Strains, and Their Susceptibilities to Induced Lysins and to Mitomycin C
journal, May 1973


DNA sequence of genes 38 encoding a receptor-recognizing protein of bacteriophages T2, K3 and of K3 host range mutants
journal, March 1987


Basic concepts in RNA virus evolution.
journal, June 1996