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Title: Host cells and methods for producing diacid compounds

Abstract

The present invention provides for a method of producing one or more fatty acid derived dicarboxylic acids in a genetically modified host cell which does not naturally produce the one or more derived fatty acid derived dicarboxylic acids. The invention provides for the biosynthesis of dicarboxylic acid ranging in length from C3 to C26. The host cell can be further modified to increase fatty acid production or export of the desired fatty acid derived compound, and/or decrease fatty acid storage or metabolism.

Inventors:
; ; ;
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1436519
Patent Number(s):
9,951,345
Application Number:
13/900,397
Assignee:
The Regents of the University of California (Oakland, CA) LBNL
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 May 22
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Steen, Eric J., Fortman, Jeffrey L., Dietrich, Jeffrey A., and Keasling, Jay D. Host cells and methods for producing diacid compounds. United States: N. p., 2018. Web.
Steen, Eric J., Fortman, Jeffrey L., Dietrich, Jeffrey A., & Keasling, Jay D. Host cells and methods for producing diacid compounds. United States.
Steen, Eric J., Fortman, Jeffrey L., Dietrich, Jeffrey A., and Keasling, Jay D. Tue . "Host cells and methods for producing diacid compounds". United States. https://www.osti.gov/servlets/purl/1436519.
@article{osti_1436519,
title = {Host cells and methods for producing diacid compounds},
author = {Steen, Eric J. and Fortman, Jeffrey L. and Dietrich, Jeffrey A. and Keasling, Jay D.},
abstractNote = {The present invention provides for a method of producing one or more fatty acid derived dicarboxylic acids in a genetically modified host cell which does not naturally produce the one or more derived fatty acid derived dicarboxylic acids. The invention provides for the biosynthesis of dicarboxylic acid ranging in length from C3 to C26. The host cell can be further modified to increase fatty acid production or export of the desired fatty acid derived compound, and/or decrease fatty acid storage or metabolism.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {4}
}

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Works referenced in this record:

Rational re-design of the substrate binding site of flavocytochrome P450 BM3
journal, December 2000


Characterization of Rabbit Liver Cytochrome P-450 (Laurate ?-1 Hydroxylase) Synthesized in Transformed Yeast Cells
journal, January 1988


A Single Mutation in Cytochrome P450 BM3 Changes Substrate Orientation in a Catalytic Intermediate and the Regiospecificity of Hydroxylation
journal, February 1997

  • Oliver, Catherine F.; Modi, Sandeep; Sutcliffe, Michael J.
  • Biochemistry, Vol. 36, Issue 7, p. 1567-1572
  • DOI: 10.1021/bi962826c

Production of high levels of 8:0 and 10:0 fatty acids in transgenic canola by overexpression of Ch FatB2, a thioesterase cDNA from Cuphea hookeriana
journal, February 1996


Modification of the substrate specificity of an acyl-acyl carrier protein thioesterase by protein engineering.
journal, November 1995

  • Yuan, L.; Voelker, T. A.; Hawkins, D. J.
  • Proceedings of the National Academy of Sciences, Vol. 92, Issue 23, p. 10639-10643
  • DOI: 10.1073/pnas.92.23.10639

Analyzing Lipid Metabolism: Activation and ?-Oxidation of Fatty Acids
book, January 2009


Storage Lipid Synthesis Is Non-essential in Yeast
journal, December 2001

  • Sandager, Line; Gustavsson, Maria H.; St�hl, Ulf
  • Journal of Biological Chemistry, Vol. 277, Issue 8, p. 6478-6482
  • DOI: 10.1074/jbc.M109109200