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Title: Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control

Abstract

Highly simplified lateral flow chromatographic nucleic acid sample preparation methods, devices, and integrated systems are provided for the efficient concentration of trace samples and the removal of nucleic acid amplification inhibitors. Methods for capturing and reducing inhibitors of nucleic acid amplification reactions, such as humic acid, using polyvinylpyrrolidone treated elements of the lateral flow device are also provided. Further provided are passive fluid control methods and systems for use in lateral flow assays.

Inventors:
Issue Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1435781
Patent Number(s):
9944922
Application Number:
14/962,967
Assignee:
Los Alamos National Security, LLC (Los Alamos, NM)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC52-06NA25396
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015 Dec 08
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Cary, Robert B. Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control. United States: N. p., 2018. Web.
Cary, Robert B. Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control. United States.
Cary, Robert B. Tue . "Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control". United States. https://www.osti.gov/servlets/purl/1435781.
@article{osti_1435781,
title = {Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control},
author = {Cary, Robert B.},
abstractNote = {Highly simplified lateral flow chromatographic nucleic acid sample preparation methods, devices, and integrated systems are provided for the efficient concentration of trace samples and the removal of nucleic acid amplification inhibitors. Methods for capturing and reducing inhibitors of nucleic acid amplification reactions, such as humic acid, using polyvinylpyrrolidone treated elements of the lateral flow device are also provided. Further provided are passive fluid control methods and systems for use in lateral flow assays.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {4}
}

Patent:

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