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Title: Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control


Highly simplified lateral flow chromatographic nucleic acid sample preparation methods, devices, and integrated systems are provided for the efficient concentration of trace samples and the removal of nucleic acid amplification inhibitors. Methods for capturing and reducing inhibitors of nucleic acid amplification reactions, such as humic acid, using polyvinylpyrrolidone treated elements of the lateral flow device are also provided. Further provided are passive fluid control methods and systems for use in lateral flow assays.

Issue Date:
Research Org.:
Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
OSTI Identifier:
Patent Number(s):
Application Number:
Los Alamos National Security, LLC (Los Alamos, NM)
Patent Classifications (CPCs):
DOE Contract Number:  
Resource Type:
Resource Relation:
Patent File Date: 2015 Dec 08
Country of Publication:
United States

Citation Formats

Cary, Robert B. Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control. United States: N. p., 2018. Web.
Cary, Robert B. Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control. United States.
Cary, Robert B. Tue . "Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control". United States.
title = {Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control},
author = {Cary, Robert B.},
abstractNote = {Highly simplified lateral flow chromatographic nucleic acid sample preparation methods, devices, and integrated systems are provided for the efficient concentration of trace samples and the removal of nucleic acid amplification inhibitors. Methods for capturing and reducing inhibitors of nucleic acid amplification reactions, such as humic acid, using polyvinylpyrrolidone treated elements of the lateral flow device are also provided. Further provided are passive fluid control methods and systems for use in lateral flow assays.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {4}

Works referenced in this record:

Shape matters
journal, March 2003

Helicase-dependent isothermal DNA amplification
journal, August 2004

Transmission of the Citrus Variegated Chlorosis Bacterium Xylella fastidiosa with the Sharpshooter Oncometopia nigricans
journal, November 2002

Genetic Diversity of Pierce's Disease Strains and Other Pathotypes of Xylella fastidiosa
journal, February 2001

Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports
journal, January 1994

A Universal Nucleic Acid Sequence Biosensor with Nanomolar Detection Limits
journal, February 2004

Isothermal reactions for the amplification of oligonucleotides
journal, April 2003

A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection
journal, September 1992

Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication.
journal, March 1990

Helicase structure and mechanism
journal, February 2002

Fluorescent Amplified Fragment Length Polymorphism Analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis Isolates
journal, February 2004

Potent and nontoxic antisense oligonucleotides containing locked nucleic acids
journal, May 2000

Microarray technology – an intellectual property retrospective
journal, September 2003

DMSO and Betaine Greatly Improve Amplification of GC-Rich Constructs in De Novo Synthesis
journal, June 2010

Fidelity of DNA polymerases in DNA amplification.
journal, December 1989

Design, biochemical, biophysical and biological properties of cooperative antisense oligonucleotides
journal, January 1995

Multiplex diagnostic platforms for detection of biothreat agents
journal, November 2004

Identification of A/H5N1 Influenza Viruses Using a Single Gene Diagnostic Microarray
journal, January 2007

Formamide can dramatically improve the specificity of PCR
journal, January 1990

Are We Ready for Pandemic Influenza?
journal, November 2003

Chip PCR. I. Surface passivation of microfabricated silicon-glass chips for PCR
journal, January 1996

Optimization of DNA-tagged dye-encapsulating liposomes for lateral-flow assays based on sandwich hybridization
journal, August 2006

Membrane chromatography - an integrative concept in the downstream processing of proteins
journal, July 1995

Specific PCR detection and identification of Xylella fastidiosa strains causing citrus variegated chlorosis
journal, December 1995

DNA Microarray Technology Devices, Systems, and Applications
journal, August 2002

A novel rapid hybridization technique: paper chromatography hybridization assay (PACHA)
journal, December 1993

Influenza vaccines: present and future
journal, July 2002

Dependence of the melting temperature of DNA on salt concentration
journal, April 1965

Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system.
journal, January 1992

Enhancing PCR amplification and sequencing using DNA-binding proteins
journal, December 1994

Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare
journal, October 2005

Microchromatography and microelectrophoresis on nitrocellulose membranes
journal, May 1970

Purification and characterization of Thermus thermophilus UvrD
journal, February 2003

Fate of Pierce's Disease Strains of Xylella fastidiosa in Common Riparian Plants in California
journal, September 1999

Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides
journal, October 2003

Detection of Cell and Tissue Surface Antigens Using Up-Converting Phosphors: A New Reporter Technology
journal, February 1999

Biosensor for Dengue Virus Detection:  Sensitive, Rapid, and Serotype Specific
journal, March 2002

Encoded evidence: DNA in forensic analysis
journal, October 2004

Recovery and detection of enterovirus, hepatitis A virus and Norwalk virus in hardshell clams (Mercenaria mercenaria) by RT-PCR methods
journal, February 1999

Nucleic acid sequence-based amplification
journal, March 1991

DNA sequencing by primer walking with strings of contiguous hexamers
journal, December 1992

PCR microfluidic devices for DNA amplification
journal, May 2006

Synthesis and hybridization of a series of biotinylated oligonucleotides
journal, January 1988

A rapid biosensor for viable B. anthracis spores
journal, August 2004

Polyvinylpyrrolidone-Agarose Gel Electrophoresis Purification of Polymerase Chain Reaction-Amplifiable DNA from Soils
journal, January 1993

Diagnostic applications of microarrays
journal, August 2006

journal, January 2003

Localized microwave heating in microwells for parallel DNA amplification applications
journal, February 2009

Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format.
journal, February 1989

An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA
journal, July 2000

Rapid single-tube immunocapture RT-PCR for the detection of two yam potyviruses
journal, December 1997

Technology platforms for pharmacogenomic diagnostic assays
journal, September 2004

Amplification and cloning of a long RNA virus genome using immunocapture-long RT-PCR
journal, June 1997

Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model.
journal, January 1997

Thermodynamic effects of formamide on DNA stability
journal, June 1996

Virulence signatures: microarray-based approaches to discovery and analysis
journal, November 2004

Oseltamivir Resistance during Treatment of Influenza A (H5N1) Infection
journal, December 2005

A novel, sensitive detection system for high-density microarrays using dendrimer technology
journal, August 2000

Pierce's Disease Bacterium: Mechanism of Transmission by Leafhopper Vectors
journal, November 1979

Locked nucleic acid (LNA): fine-tuning the recognition of DNA and RNA
journal, January 2001

Quantitative PCR Method for Diagnosis of Citrus Bacterial Canker
journal, June 2001

Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction.
journal, January 1992

Controlled reagent transport in disposable 2D paper networks
journal, January 2010

One-step immobilization of poly(dT)-modified DNA onto non-modified plastic substrates by UV irradiation for microarrays
journal, August 2006

A simple procedure for enhancing PCR specificity.
journal, August 1993

Ligation mediated PCR performed at low denaturation temperatures--PCR melting profiles
journal, September 2003

Characterization of a Thermostable UvrD Helicase and Its Participation in Helicase-dependent Amplification
journal, June 2005

Lab-on-a-chip devices for global health: Past studies and future opportunities
journal, January 2007

An Integrated Nanoliter DNA Analysis Device
journal, October 1998

Automated closed-vessel system for in vitro diagnostics based on polymerase chain reaction
journal, September 1993

Immunoassay of infectious agents
journal, October 2003

Strand displacement amplification—an isothermal, in vitro DNA amplification technique
journal, January 1992

Biosensors for environmental pollutants and food contaminants
journal, October 2003

Capture of Single-Stranded DNA Assisted by Oligonucleotide Modules
journal, January 1998

Advances in Polymerase Chain Reaction on Microfluidic Chips
journal, June 2005

Early diagnosis of bubonic plague using F1 antigen capture ELISA assay and rapid immunogold dipstick
journal, July 2000

Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
journal, January 1988

DnaB from Thermus aquaticus Unwinds Forked Duplex DNA with an Asymmetric Tail Length Dependence
journal, March 1999

Development of a multiplex immunocapture RT-PCR assay for detection and differentiation of tomato and tobacco mosaic tobamoviruses
journal, October 1998

The total influenza vaccine failure of 1947 revisited: Major intrasubtypic antigenic change can explain failure of vaccine in a post-World War II epidemic
journal, July 2002

Detection and Diversity Assessment of Xylella fastidiosa in Field-Collected Plant and Insect Samples by Using 16S rRNA and gyrB Sequences
journal, July 2003

Pierce's Disease of Grapevines: Isolation of the Causal Bacterium
journal, January 1978

Inhibition and facilitation of nucleic acid amplification.
journal, January 1997

Xylella Fastidiosa: Xylem-Limited Bacterial Pathogen of Plants
journal, September 1989

Multi-analyte single-membrane biosensor for the serotype-specific detection of Dengue virus
journal, July 2004

An efficient and economic enhancer mix for PCR
journal, September 2006

PCR in a silicon microstructure
journal, September 1994

Chemiluminescence Detection for Hybridization Assays on the Flow-Thru Chip, a Three-Dimensional Microchannel Biochip
journal, December 2001

Effects of Amplification Facilitators on Diagnostic PCR in the Presence of Blood, Feces, and Meat
journal, January 2000

Detection of Xanthomonas campestris pv. citri by the polymerase chain reaction method.
journal, January 1993

Using a microfluidic device for 1  l DNA microarray hybridization in 500 s
journal, April 2005

Direct detection of potato leafroll virus in potato tubers by immunocapture and the isothermal nucleic acid amplification method NASBA
journal, June 1997

Chromatographic performance of a thin microporous bed of nitrocellulose
journal, November 2001

Design Strategies and Performance of Custom DNA Sequencing Primers
journal, September 1999

The ligase chain reaction in a PCR world.
journal, August 1991

Biosensor for the specific detection of a single viable B. anthracis spore
journal, May 2003

Potential Impact of Antiviral Drug Use during Influenza Pandemic
journal, September 2005

Immunoaffinity concentration and purification of waterborne enteric viruses for detection by reverse transcriptase PCR.
journal, January 1996

Rapid, direct extraction of DNA from soils for PCR analysis using polyvinylpolypyrrolidone spin columns
journal, April 1996

Droplet-based micro oscillating-flow PCR chip
journal, May 2005

A nested-PCR assay for detection of Xylella fastidiosa in citrus plants and sharpshooter leafhoppers
journal, March 2004

Developing rapid, point-of-care, multiplex detection for use in lateral flow devices
conference, November 2005

DNA Detection Using Recombination Proteins
journal, June 2006

A coupled complex of T4 DNA replication helicase (gp41) and polymerase (gp43) can perform rapid and processive DNA strand-displacement synthesis
journal, December 1996

Detection of amantadine-resistant variants among avian influenza viruses isolated in North America and Asia
journal, October 2005

Molecular Crowding Regulates the Structural Switch of the DNA G-Quadruplex
journal, December 2002

Betaine, Dimethyl Sulfoxide, and 7-Deaza-dGTP, a Powerful Mixture for Amplification of GC-Rich DNA Sequences
journal, November 2006

RNA replication: required intermediates and the dissociation of template, product, and Q.beta. replicase
journal, May 1979

Evaluation of NucliSens easyMAG for Automated Nucleic Acid Extraction from Various Clinical Specimens
journal, December 2006

Detection of foodborne pathogens using DNA probes and a dipstick format
journal, December 1996

Immobilization of polynucleotides on magnetic particles. Factors influencing hybridization efficiency
journal, September 1991

Rapid and simple method for purification of nucleic acids.
journal, January 1990

Inclusion of polyvinylpyrrolidone in the polymerase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA
journal, February 1999

Multiplex PCR: Critical Parameters and Step-by-Step Protocol
journal, September 1997

Point-of-care diagnostics: will the hurdles be overcome this time?
journal, July 2006

Rapid method for visual identification of specific DNA sequences based on DNA-tagged liposomes
journal, August 1996

DNA analysis with multiplex microarray-enhanced PCR
journal, January 2005

journal, November 2003

Miniature RT-PCR system for diagnosis of RNA-based viruses
journal, October 2005

Exponential DNA Replication by Laminar Convection
journal, October 2003

Rapid purification of hepatitis B virus DNA from serum.
journal, January 1991

A ligase-mediated gene detection technique
journal, August 1988

Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content.
journal, July 1996

    Works referencing / citing this record:

    Specific Bonding Analysis Method and Specific Bonding Analysis Device Using It
    patent-application, May 2003

    Pharmaceutical composition containing a stabilised mRNA optimised for translation in its coding regions
    patent-application, February 2005

    Rapid screening assay methods and devices
    patent, July 1999

    Amelioration of Heterophile Antibody Immunosensor Interference
    patent-application, September 2010

    Lateral Flow Diagnostic Devices with Instrument Controlled Fluidics
    patent-application, March 2005

    Microwave Assisted PCR Amplification of DNA
    patent-application, October 2007

    Diagnostic marker for tumor hypoxia and prognosis
    patent-application, March 2003

    Liquid transfer devices
    patent, October 1994

    Method for extending the dynamic detection range of assay devices
    patent-application, May 2005

    Method and Apparatus for Amplification of Nucleic Acid Sequences by Using Thermal Convection
    patent-application, August 2004

    Nucleic acid detection system
    patent-application, October 2005

    Methods and Compositions for Polynucleotide Amplification
    patent-application, February 2005

    Devices and Methods for Biochip Multiplexing
    patent-application, November 2002

    Chromatographic test strip for determining ligands or receptors
    patent, October 1990

    Use of photopolymerization for amplification and detection of a molecular recognition event
    patent-application, December 2006

    Gravity-driven micropump
    patent, May 2012

    Sequence-dependent gene sorting techniques
    patent, October 2002

    Test device and method for its use
    patent, November 1980

    Nanoparticle Probes with Raman Spectroscopic Fingerprints for Analyte Detection
    patent-application, May 2004

    Assay devices having detection capabilities within the hook effect region
    patent-application, November 2006

    Isothermal strand displacement nucleic acid amplification
    patent, April 2001

    Barriers for Facilitating Biological Reactions
    patent-application, October 2009

    Mobile Rapid Test System for Nucleic Acid Analysis
    patent-application, February 2011

    Pumpless microfluidics
    patent, June 2004

    Electrically opened micro fluid valve
    patent, January 2007

    Generation and amplification of nucleic acids from ribonucleic acids
    patent, October 2001

    Micro-arrayed organization of transcription factor target genes
    patent-application, April 2005

    Modified Substrates for the Attachment of Biomolecules
    patent-application, March 2003

    Solid supports for nucleic acid hybridization assays
    patent, May 1996

    Method for Amplification of DNA
    patent-application, September 2001

    Systems and Methods for Testing Using Microfluidic Chips
    patent-application, November 2008

    Integrated biochip system for sample preparation and analysis
    patent-application, June 2002

    Single Use Microfluidic Valve
    patent-application, November 2010

    Process for amplifying, detecting, and/or-cloning nucleic acid sequences
    patent, July 1987

    Printed fluid transport devices
    patent, April 1998

    Chromatographic Material
    patent, June 1972

    Lateral flow system for nucleic acid detection
    patent-application, June 2004

    Microfluidic Flow Monitoring Device
    patent-application, February 2007

    Capture and detection of target nucleic acid in dipstick assays
    patent, March 2007

    Enrichment of a Target Sequence
    patent-application, August 2010

    Microfluidic devices comprising biochannels
    patent-application, October 2003

    Lateral flow assay device and method
    patent-application, July 2006

    High surface area substrates for microarrays and methods to make same
    patent, March 2007

    Multiple Hybrid Immunoassay
    patent-application, May 2014

    Process for amplifying, detecting, and/or cloning nucleic acid sequences
    patent, January 1989

    Two step lateral flow assay methods and devices
    patent-application, January 2007

    Controlled flow assay device and method
    patent-application, October 2006

    Microfluidic Cartridge
    patent-application, May 2009

    Systems and methods for detection of analytes in biological fluids
    patent-application, January 2003

    Biosensor and Measurement Method
    patent-application, February 2004

    Autonomous Microfluidic Apparatus
    patent-application, February 2009

    Collection device for single step assay of oral fluids
    patent-application, December 2002

    Method of adjusting the working range of a multi-analyte assay
    patent-application, August 2006

    Multistep Reaction Lateral Flow Capillary Device
    patent-application, June 2008

    Helicase dependent amplification of nucleic acids
    patent-application, March 2004

    Capillary flow liquid transfer device having waste reception area
    patent, April 1997

    Transmission-based optical detection systems
    patent-application, November 2005

    Displacement assay on a porous membrane
    patent-application, March 2002

    Diagnostic assays including multiplexed lateral flow immunoassays with quantum dots
    patent-application, November 2005

    Sample-efficient lateral flow immunoassay
    patent-application, June 2006

    Nucleic acid analysis techniques
    patent-application, April 2003

    Nano-mechanic microsensors and methods for detecting target analytes
    patent-application, February 2006

    Methods and Devices for Microfluidic Point-of-Care Immunoassays
    patent-application, July 2009

    Methods and compositions for amplification of RNA sequences
    patent-application, January 2005

    Self-Contained Microfluidic Biochip and Apparatus
    patent-application, October 2005

    Chip-based isothermal amplification devices and methods
    patent-application, November 2002

    Nucleic acid amplification utilizing microfluidic devices
    patent-application, January 2003

    Method of Sample Analysis and Apparatus Therefor
    patent-application, May 2008

    Nucleic Acid Detection System and Method for Detecting Influenza
    patent-application, July 2015

    Nucleic Acid Amplification Method
    patent-application, June 2005

    Detection Signal and Capture in Dipstick Assays
    patent-application, March 2004

    Asymmetrically branched polymer conjugates and microarray assays
    patent-application, February 2006

    Oxygen sensing membranes and methods of making same
    patent, February 2001

    Method and apparatus for washing magnetically responsive particles
    patent-application, September 2003