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Title: Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control

Abstract

Highly simplified lateral flow chromatographic nucleic acid sample preparation methods, devices, and integrated systems are provided for the efficient concentration of trace samples and the removal of nucleic acid amplification inhibitors. Methods for capturing and reducing inhibitors of nucleic acid amplification reactions, such as humic acid, using polyvinylpyrrolidone treated elements of the lateral flow device are also provided. Further provided are passive fluid control methods and systems for use in lateral flow assays.

Inventors:
Issue Date:
Research Org.:
Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1435781
Patent Number(s):
9944922
Application Number:
14/962,967
Assignee:
Los Alamos National Security, LLC (Los Alamos, NM)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
AC52-06NA25396
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015 Dec 08
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Cary, Robert B. Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control. United States: N. p., 2018. Web.
Cary, Robert B. Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control. United States.
Cary, Robert B. Tue . "Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control". United States. https://www.osti.gov/servlets/purl/1435781.
@article{osti_1435781,
title = {Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control},
author = {Cary, Robert B.},
abstractNote = {Highly simplified lateral flow chromatographic nucleic acid sample preparation methods, devices, and integrated systems are provided for the efficient concentration of trace samples and the removal of nucleic acid amplification inhibitors. Methods for capturing and reducing inhibitors of nucleic acid amplification reactions, such as humic acid, using polyvinylpyrrolidone treated elements of the lateral flow device are also provided. Further provided are passive fluid control methods and systems for use in lateral flow assays.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {4}
}

Works referenced in this record:

Shape matters
journal, March 2003


Helicase-dependent isothermal DNA amplification
journal, August 2004


Transmission of the Citrus Variegated Chlorosis Bacterium Xylella fastidiosa with the Sharpshooter Oncometopia nigricans
journal, November 2002


Genetic Diversity of Pierce's Disease Strains and Other Pathotypes of Xylella fastidiosa
journal, February 2001


Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports
journal, January 1994


A Universal Nucleic Acid Sequence Biosensor with Nanomolar Detection Limits
journal, February 2004


Isothermal reactions for the amplification of oligonucleotides
journal, April 2003


A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection
journal, September 1992


Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication.
journal, March 1990


Helicase structure and mechanism
journal, February 2002


Fluorescent Amplified Fragment Length Polymorphism Analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis Isolates
journal, February 2004


Potent and nontoxic antisense oligonucleotides containing locked nucleic acids
journal, May 2000


Microarray technology – an intellectual property retrospective
journal, September 2003


DMSO and Betaine Greatly Improve Amplification of GC-Rich Constructs in De Novo Synthesis
journal, June 2010


Fidelity of DNA polymerases in DNA amplification.
journal, December 1989


Design, biochemical, biophysical and biological properties of cooperative antisense oligonucleotides
journal, January 1995


Multiplex diagnostic platforms for detection of biothreat agents
journal, November 2004


Identification of A/H5N1 Influenza Viruses Using a Single Gene Diagnostic Microarray
journal, January 2007


Formamide can dramatically improve the specificity of PCR
journal, January 1990


Are We Ready for Pandemic Influenza?
journal, November 2003


Chip PCR. I. Surface passivation of microfabricated silicon-glass chips for PCR
journal, January 1996


Optimization of DNA-tagged dye-encapsulating liposomes for lateral-flow assays based on sandwich hybridization
journal, August 2006


Membrane chromatography - an integrative concept in the downstream processing of proteins
journal, July 1995


Specific PCR detection and identification of Xylella fastidiosa strains causing citrus variegated chlorosis
journal, December 1995


DNA Microarray Technology Devices, Systems, and Applications
journal, August 2002


A novel rapid hybridization technique: paper chromatography hybridization assay (PACHA)
journal, December 1993


Influenza vaccines: present and future
journal, July 2002


Dependence of the melting temperature of DNA on salt concentration
journal, April 1965


Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system.
journal, January 1992


Enhancing PCR amplification and sequencing using DNA-binding proteins
journal, December 1994


Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare
journal, October 2005


Microchromatography and microelectrophoresis on nitrocellulose membranes
journal, May 1970


Purification and characterization of Thermus thermophilus UvrD
journal, February 2003


Fate of Pierce's Disease Strains of Xylella fastidiosa in Common Riparian Plants in California
journal, September 1999


Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides
journal, October 2003


Detection of Cell and Tissue Surface Antigens Using Up-Converting Phosphors: A New Reporter Technology
journal, February 1999


Biosensor for Dengue Virus Detection:  Sensitive, Rapid, and Serotype Specific
journal, March 2002


Encoded evidence: DNA in forensic analysis
journal, October 2004


Recovery and detection of enterovirus, hepatitis A virus and Norwalk virus in hardshell clams (Mercenaria mercenaria) by RT-PCR methods
journal, February 1999


Nucleic acid sequence-based amplification
journal, March 1991


DNA sequencing by primer walking with strings of contiguous hexamers
journal, December 1992


PCR microfluidic devices for DNA amplification
journal, May 2006


Synthesis and hybridization of a series of biotinylated oligonucleotides
journal, January 1988


A rapid biosensor for viable B. anthracis spores
journal, August 2004


Polyvinylpyrrolidone-Agarose Gel Electrophoresis Purification of Polymerase Chain Reaction-Amplifiable DNA from Soils
journal, January 1993


Diagnostic applications of microarrays
journal, August 2006


Influenza
journal, January 2003


Localized microwave heating in microwells for parallel DNA amplification applications
journal, February 2009


Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format.
journal, February 1989


An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA
journal, July 2000


Rapid single-tube immunocapture RT-PCR for the detection of two yam potyviruses
journal, December 1997


Technology platforms for pharmacogenomic diagnostic assays
journal, September 2004


Amplification and cloning of a long RNA virus genome using immunocapture-long RT-PCR
journal, June 1997


Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model.
journal, January 1997


Thermodynamic effects of formamide on DNA stability
journal, June 1996


Virulence signatures: microarray-based approaches to discovery and analysis
journal, November 2004


Oseltamivir Resistance during Treatment of Influenza A (H5N1) Infection
journal, December 2005


A novel, sensitive detection system for high-density microarrays using dendrimer technology
journal, August 2000


Pierce's Disease Bacterium: Mechanism of Transmission by Leafhopper Vectors
journal, November 1979


Locked nucleic acid (LNA): fine-tuning the recognition of DNA and RNA
journal, January 2001


Quantitative PCR Method for Diagnosis of Citrus Bacterial Canker
journal, June 2001


Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction.
journal, January 1992


Controlled reagent transport in disposable 2D paper networks
journal, January 2010


One-step immobilization of poly(dT)-modified DNA onto non-modified plastic substrates by UV irradiation for microarrays
journal, August 2006


A simple procedure for enhancing PCR specificity.
journal, August 1993


Ligation mediated PCR performed at low denaturation temperatures--PCR melting profiles
journal, September 2003


Characterization of a Thermostable UvrD Helicase and Its Participation in Helicase-dependent Amplification
journal, June 2005


Lab-on-a-chip devices for global health: Past studies and future opportunities
journal, January 2007


An Integrated Nanoliter DNA Analysis Device
journal, October 1998


Automated closed-vessel system for in vitro diagnostics based on polymerase chain reaction
journal, September 1993


Immunoassay of infectious agents
journal, October 2003


Strand displacement amplification—an isothermal, in vitro DNA amplification technique
journal, January 1992


Biosensors for environmental pollutants and food contaminants
journal, October 2003


Capture of Single-Stranded DNA Assisted by Oligonucleotide Modules
journal, January 1998


Advances in Polymerase Chain Reaction on Microfluidic Chips
journal, June 2005


Early diagnosis of bubonic plague using F1 antigen capture ELISA assay and rapid immunogold dipstick
journal, July 2000


Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
journal, January 1988


DnaB from Thermus aquaticus Unwinds Forked Duplex DNA with an Asymmetric Tail Length Dependence
journal, March 1999


Development of a multiplex immunocapture RT-PCR assay for detection and differentiation of tomato and tobacco mosaic tobamoviruses
journal, October 1998


The total influenza vaccine failure of 1947 revisited: Major intrasubtypic antigenic change can explain failure of vaccine in a post-World War II epidemic
journal, July 2002


Detection and Diversity Assessment of Xylella fastidiosa in Field-Collected Plant and Insect Samples by Using 16S rRNA and gyrB Sequences
journal, July 2003


Pierce's Disease of Grapevines: Isolation of the Causal Bacterium
journal, January 1978


Inhibition and facilitation of nucleic acid amplification.
journal, January 1997


Xylella Fastidiosa: Xylem-Limited Bacterial Pathogen of Plants
journal, September 1989


Multi-analyte single-membrane biosensor for the serotype-specific detection of Dengue virus
journal, July 2004


An efficient and economic enhancer mix for PCR
journal, September 2006


PCR in a silicon microstructure
journal, September 1994


Chemiluminescence Detection for Hybridization Assays on the Flow-Thru Chip, a Three-Dimensional Microchannel Biochip
journal, December 2001


Effects of Amplification Facilitators on Diagnostic PCR in the Presence of Blood, Feces, and Meat
journal, January 2000


Detection of Xanthomonas campestris pv. citri by the polymerase chain reaction method.
journal, January 1993


Using a microfluidic device for 1  l DNA microarray hybridization in 500 s
journal, April 2005


Direct detection of potato leafroll virus in potato tubers by immunocapture and the isothermal nucleic acid amplification method NASBA
journal, June 1997


Chromatographic performance of a thin microporous bed of nitrocellulose
journal, November 2001


Design Strategies and Performance of Custom DNA Sequencing Primers
journal, September 1999


The ligase chain reaction in a PCR world.
journal, August 1991


Biosensor for the specific detection of a single viable B. anthracis spore
journal, May 2003


Potential Impact of Antiviral Drug Use during Influenza Pandemic
journal, September 2005


Immunoaffinity concentration and purification of waterborne enteric viruses for detection by reverse transcriptase PCR.
journal, January 1996


Rapid, direct extraction of DNA from soils for PCR analysis using polyvinylpolypyrrolidone spin columns
journal, April 1996


Droplet-based micro oscillating-flow PCR chip
journal, May 2005


A nested-PCR assay for detection of Xylella fastidiosa in citrus plants and sharpshooter leafhoppers
journal, March 2004


Developing rapid, point-of-care, multiplex detection for use in lateral flow devices
conference, November 2005


DNA Detection Using Recombination Proteins
journal, June 2006


A coupled complex of T4 DNA replication helicase (gp41) and polymerase (gp43) can perform rapid and processive DNA strand-displacement synthesis
journal, December 1996


Detection of amantadine-resistant variants among avian influenza viruses isolated in North America and Asia
journal, October 2005


Molecular Crowding Regulates the Structural Switch of the DNA G-Quadruplex
journal, December 2002


Betaine, Dimethyl Sulfoxide, and 7-Deaza-dGTP, a Powerful Mixture for Amplification of GC-Rich DNA Sequences
journal, November 2006


RNA replication: required intermediates and the dissociation of template, product, and Q.beta. replicase
journal, May 1979


Evaluation of NucliSens easyMAG for Automated Nucleic Acid Extraction from Various Clinical Specimens
journal, December 2006


Detection of foodborne pathogens using DNA probes and a dipstick format
journal, December 1996


Immobilization of polynucleotides on magnetic particles. Factors influencing hybridization efficiency
journal, September 1991


Rapid and simple method for purification of nucleic acids.
journal, January 1990


Inclusion of polyvinylpyrrolidone in the polymerase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA
journal, February 1999


Multiplex PCR: Critical Parameters and Step-by-Step Protocol
journal, September 1997


Point-of-care diagnostics: will the hurdles be overcome this time?
journal, July 2006


Rapid method for visual identification of specific DNA sequences based on DNA-tagged liposomes
journal, August 1996


DNA analysis with multiplex microarray-enhanced PCR
journal, January 2005


Influenza
journal, November 2003


Miniature RT-PCR system for diagnosis of RNA-based viruses
journal, October 2005


Exponential DNA Replication by Laminar Convection
journal, October 2003


Rapid purification of hepatitis B virus DNA from serum.
journal, January 1991


A ligase-mediated gene detection technique
journal, August 1988


Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content.
journal, July 1996


    Works referencing / citing this record:

    Specific Bonding Analysis Method and Specific Bonding Analysis Device Using It
    patent-application, May 2003


    Pharmaceutical composition containing a stabilised mRNA optimised for translation in its coding regions
    patent-application, February 2005


    Rapid screening assay methods and devices
    patent, July 1999


    Amelioration of Heterophile Antibody Immunosensor Interference
    patent-application, September 2010


    Lateral Flow Diagnostic Devices with Instrument Controlled Fluidics
    patent-application, March 2005


    Microwave Assisted PCR Amplification of DNA
    patent-application, October 2007


    Diagnostic marker for tumor hypoxia and prognosis
    patent-application, March 2003


    Liquid transfer devices
    patent, October 1994


    Method for extending the dynamic detection range of assay devices
    patent-application, May 2005


    Method and Apparatus for Amplification of Nucleic Acid Sequences by Using Thermal Convection
    patent-application, August 2004


    Nucleic acid detection system
    patent-application, October 2005


    Methods and Compositions for Polynucleotide Amplification
    patent-application, February 2005


    Devices and Methods for Biochip Multiplexing
    patent-application, November 2002


    Chromatographic test strip for determining ligands or receptors
    patent, October 1990


    Use of photopolymerization for amplification and detection of a molecular recognition event
    patent-application, December 2006


    Gravity-driven micropump
    patent, May 2012


    Sequence-dependent gene sorting techniques
    patent, October 2002


    Test device and method for its use
    patent, November 1980


    Nanoparticle Probes with Raman Spectroscopic Fingerprints for Analyte Detection
    patent-application, May 2004


    Assay devices having detection capabilities within the hook effect region
    patent-application, November 2006


    Isothermal strand displacement nucleic acid amplification
    patent, April 2001


    Barriers for Facilitating Biological Reactions
    patent-application, October 2009


    Mobile Rapid Test System for Nucleic Acid Analysis
    patent-application, February 2011


    Pumpless microfluidics
    patent, June 2004


    Electrically opened micro fluid valve
    patent, January 2007


    Generation and amplification of nucleic acids from ribonucleic acids
    patent, October 2001


    Micro-arrayed organization of transcription factor target genes
    patent-application, April 2005


    Modified Substrates for the Attachment of Biomolecules
    patent-application, March 2003


    Solid supports for nucleic acid hybridization assays
    patent, May 1996


    Method for Amplification of DNA
    patent-application, September 2001


    Systems and Methods for Testing Using Microfluidic Chips
    patent-application, November 2008


    Integrated biochip system for sample preparation and analysis
    patent-application, June 2002


    Single Use Microfluidic Valve
    patent-application, November 2010


    Process for amplifying, detecting, and/or-cloning nucleic acid sequences
    patent, July 1987


    Printed fluid transport devices
    patent, April 1998


    Chromatographic Material
    patent, June 1972


    Lateral flow system for nucleic acid detection
    patent-application, June 2004


    Microfluidic Flow Monitoring Device
    patent-application, February 2007


    Capture and detection of target nucleic acid in dipstick assays
    patent, March 2007


    Enrichment of a Target Sequence
    patent-application, August 2010


    Microfluidic devices comprising biochannels
    patent-application, October 2003


    Lateral flow assay device and method
    patent-application, July 2006


    High surface area substrates for microarrays and methods to make same
    patent, March 2007


    Multiple Hybrid Immunoassay
    patent-application, May 2014


    Process for amplifying, detecting, and/or cloning nucleic acid sequences
    patent, January 1989


    Two step lateral flow assay methods and devices
    patent-application, January 2007


    Controlled flow assay device and method
    patent-application, October 2006


    Microfluidic Cartridge
    patent-application, May 2009


    Systems and methods for detection of analytes in biological fluids
    patent-application, January 2003


    Biosensor and Measurement Method
    patent-application, February 2004


    Autonomous Microfluidic Apparatus
    patent-application, February 2009


    Collection device for single step assay of oral fluids
    patent-application, December 2002


    Method of adjusting the working range of a multi-analyte assay
    patent-application, August 2006


    Multistep Reaction Lateral Flow Capillary Device
    patent-application, June 2008


    Helicase dependent amplification of nucleic acids
    patent-application, March 2004


    Capillary flow liquid transfer device having waste reception area
    patent, April 1997


    Transmission-based optical detection systems
    patent-application, November 2005


    Displacement assay on a porous membrane
    patent-application, March 2002


    Diagnostic assays including multiplexed lateral flow immunoassays with quantum dots
    patent-application, November 2005


    Sample-efficient lateral flow immunoassay
    patent-application, June 2006


    Nucleic acid analysis techniques
    patent-application, April 2003


    Nano-mechanic microsensors and methods for detecting target analytes
    patent-application, February 2006


    Methods and Devices for Microfluidic Point-of-Care Immunoassays
    patent-application, July 2009


    Methods and compositions for amplification of RNA sequences
    patent-application, January 2005


    Self-Contained Microfluidic Biochip and Apparatus
    patent-application, October 2005


    Chip-based isothermal amplification devices and methods
    patent-application, November 2002


    Nucleic acid amplification utilizing microfluidic devices
    patent-application, January 2003


    Method of Sample Analysis and Apparatus Therefor
    patent-application, May 2008


    Nucleic Acid Detection System and Method for Detecting Influenza
    patent-application, July 2015


    Nucleic Acid Amplification Method
    patent-application, June 2005


    Detection Signal and Capture in Dipstick Assays
    patent-application, March 2004


    Asymmetrically branched polymer conjugates and microarray assays
    patent-application, February 2006


    Oxygen sensing membranes and methods of making same
    patent, February 2001


    Method and apparatus for washing magnetically responsive particles
    patent-application, September 2003