Title: Use of galerina marginata genes and proteins for peptide production

Abstract

The present invention relates to compositions and methods comprising genes and peptides associated with cyclic peptides and cyclic peptide production in mushrooms. In particular, the present invention relates to using genes and proteins from Galerina species encoding peptides specifically relating to amatoxins in addition to proteins involved with processing cyclic peptide toxins. In a preferred embodiment, the present invention also relates to methods for making small peptides and small cyclic peptides including peptides similar to amanitin. Further, the present inventions relate to providing kits for making small peptides.

Inventors:

Hallen-Adams, Heather E.; Scott-Craig, John S.; Walton, Jonathan D.; Luo, Hong

Issue Date:

2018-04-03

Research Org.:

Michigan State Univ., East Lansing, MI (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1433343

Patent Number(s):

9932571

Application Number:

15/437,359

Assignee:

Board of Trustees of Michigan State University (East Lansing, MI)

Patent Classifications (CPCs):

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K14/375 - from Basidiomycetes
C07K7/64 - Cyclic peptides containing only normal peptide links

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N9/58 - derived from fungi

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y304/21026 - Prolyl oligopeptidase

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P21/06 - produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

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DOE Contract Number:  

FG02-91ER20021

Resource Type:

Patent

Resource Relation:

Patent File Date: 2017 Feb 20

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES