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Title: Agrobacterium-mediated transformation of lipomyces

Abstract

This disclosure provides Agrobacterium-mediated transformation methods for the oil-producing (oleaginous) yeast Lipomyces sp., as well as yeast produced by the method. Such methods utilize Agrobacterium sp. cells that have a T-DNA binary plasmid, wherein the T-DNA binary plasmid comprises a first nucleic acid molecule encoding a first protein and a second nucleic acid molecule encoding a selective marker that permits growth of transformed Lipomyces sp. cells in selective culture media comprising an antibiotic.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1426818
Patent Number(s):
9914932
Application Number:
14/540,818
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Patent
Resource Relation:
Patent File Date: 2014 Nov 13
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Dai, Ziyu, Magnuson, Jon K., Deng, Shuang, Bruno, Kenneth S., and Culley, David E.. Agrobacterium-mediated transformation of lipomyces. United States: N. p., 2018. Web.
Dai, Ziyu, Magnuson, Jon K., Deng, Shuang, Bruno, Kenneth S., & Culley, David E.. Agrobacterium-mediated transformation of lipomyces. United States.
Dai, Ziyu, Magnuson, Jon K., Deng, Shuang, Bruno, Kenneth S., and Culley, David E.. Tue . "Agrobacterium-mediated transformation of lipomyces". United States. https://www.osti.gov/servlets/purl/1426818.
@article{osti_1426818,
title = {Agrobacterium-mediated transformation of lipomyces},
author = {Dai, Ziyu and Magnuson, Jon K. and Deng, Shuang and Bruno, Kenneth S. and Culley, David E.},
abstractNote = {This disclosure provides Agrobacterium-mediated transformation methods for the oil-producing (oleaginous) yeast Lipomyces sp., as well as yeast produced by the method. Such methods utilize Agrobacterium sp. cells that have a T-DNA binary plasmid, wherein the T-DNA binary plasmid comprises a first nucleic acid molecule encoding a first protein and a second nucleic acid molecule encoding a selective marker that permits growth of transformed Lipomyces sp. cells in selective culture media comprising an antibiotic.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {3}
}

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Works referenced in this record:

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Agrobacterium-Mediated Transformation of Non-Plant Organisms
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Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae.
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Agrobacterium tumefaciens-mediated transformation of filamentous fungi
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Temperature affects the T-DNA transfer machinery of Agrobacterium tumefaciens.
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The Effect of the Agrobacterium tumefaciens attR Mutation on Attachment and Root Colonization Differs between Legumes and Other Dicots
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Synergistic Action of D-Glucose and Acetosyringone on Agrobacterium Strains for Efficient Dunaliella Transformation
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