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Title: Funnel for localizing biological cell placement and arrangement

Abstract

The present disclosure relates to a funnel apparatus for channeling cells onto a plurality of distinct, closely spaced regions of a seeding surface. The funnel apparatus has a body portion having an upper surface and a lower surface. The body portion forms a plurality of flow paths, at least one of which is shaped to have a decreasing cross-sectional area from the upper surface to the lower surface. The flow paths are formed at the lower surface to enable cells deposited into the flow paths at the upper surface of the funnel apparatus to be channeled into a plurality of distinct, closely spaced regions on the seeding surface positioned adjacent the lower surface.

Inventors:
; ;
Issue Date:
Research Org.:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1425913
Patent Number(s):
9909093
Application Number:
14/824,857
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12M - APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
DOE Contract Number:  
AC52-07NA27344
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015 Aug 12
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Soscia, David, Benett, William J., and Mukerjee, Erik V. Funnel for localizing biological cell placement and arrangement. United States: N. p., 2018. Web.
Soscia, David, Benett, William J., & Mukerjee, Erik V. Funnel for localizing biological cell placement and arrangement. United States.
Soscia, David, Benett, William J., and Mukerjee, Erik V. Tue . "Funnel for localizing biological cell placement and arrangement". United States. https://www.osti.gov/servlets/purl/1425913.
@article{osti_1425913,
title = {Funnel for localizing biological cell placement and arrangement},
author = {Soscia, David and Benett, William J. and Mukerjee, Erik V.},
abstractNote = {The present disclosure relates to a funnel apparatus for channeling cells onto a plurality of distinct, closely spaced regions of a seeding surface. The funnel apparatus has a body portion having an upper surface and a lower surface. The body portion forms a plurality of flow paths, at least one of which is shaped to have a decreasing cross-sectional area from the upper surface to the lower surface. The flow paths are formed at the lower surface to enable cells deposited into the flow paths at the upper surface of the funnel apparatus to be channeled into a plurality of distinct, closely spaced regions on the seeding surface positioned adjacent the lower surface.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 06 00:00:00 EST 2018},
month = {Tue Mar 06 00:00:00 EST 2018}
}

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Large-Scale Single-Cell Trapping And Imaging Using Microwell Arrays
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3-D microwell culture of human embryonic stem cells
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