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Title: Methods and compositions for altering lignin composition in plants

Abstract

The invention provides methods for decreasing lignin content in plants by reducing expression of a folylpolyglutamate synthetase 1 (FPGS1) coding sequence in the plant. Also provided are methods for reducing lignin content in a plant by down-regulation of FPGS1 expression in the plant. Nucleic acid molecules for modulation of FPGS1 expression and transgenic plants the same are also provided. Plants described herein may be used, for example, as improved biofuel feedstock and as highly digestible forage crops. Methods for processing plant tissue and for producing biofuels by utilizing such plants are also provided.

Inventors:
; ;
Issue Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1425907
Patent Number(s):
9,909,136
Application Number:
14/710,419
Assignee:
The Samuel Roberts Noble Foundation, Inc. (Ardmore, OK) ORNL
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015 May 12
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Srivastava, Avinash C., Tang, Yuhong, and Blancaflor, Elison. Methods and compositions for altering lignin composition in plants. United States: N. p., 2018. Web.
Srivastava, Avinash C., Tang, Yuhong, & Blancaflor, Elison. Methods and compositions for altering lignin composition in plants. United States.
Srivastava, Avinash C., Tang, Yuhong, and Blancaflor, Elison. Tue . "Methods and compositions for altering lignin composition in plants". United States. https://www.osti.gov/servlets/purl/1425907.
@article{osti_1425907,
title = {Methods and compositions for altering lignin composition in plants},
author = {Srivastava, Avinash C. and Tang, Yuhong and Blancaflor, Elison},
abstractNote = {The invention provides methods for decreasing lignin content in plants by reducing expression of a folylpolyglutamate synthetase 1 (FPGS1) coding sequence in the plant. Also provided are methods for reducing lignin content in a plant by down-regulation of FPGS1 expression in the plant. Nucleic acid molecules for modulation of FPGS1 expression and transgenic plants the same are also provided. Plants described herein may be used, for example, as improved biofuel feedstock and as highly digestible forage crops. Methods for processing plant tissue and for producing biofuels by utilizing such plants are also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {3}
}

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Works referenced in this record:

Functional analysis of folate polyglutamylation and its essential role in plant metabolism and development
journal, September 2010


Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana
journal, August 2003

  • Alonso, J. M.; Stepanova, Anna N.; Leisse, Thomas J.
  • Science, Vol. 301, Issue 5633, p. 653-657
  • DOI: 10.1126/science.1086391

The Genetics of Lignin Biosynthesis: Connecting Genotype to Phenotype
journal, December 2010


Differential expression of genes involved in C1 metabolism and lignin biosynthesis in wooden core and bast tissues of fibre hemp (Cannabis sativa L.)
journal, February 2008


Quantification of plasma S-adenosylmethionine and S-adenosylhomocysteine as their fluorescent 1,N6-etheno derivatives: an adaptation of previously described methodology
journal, July 2002

  • Castro, Rita; Struys, Eduard A.; Jansen, Erwin E. W.
  • Journal of Pharmaceutical and Biomedical Analysis, Vol. 29, Issue 5, p. 963-968
  • DOI: 10.1016/S0731-7085(02)00121-8

A Gateway Cloning Vector Set for High-Throughput Functional Analysis of Genes in Planta
journal, September 2003

  • Curtis, Mark D.; Grossniklaus, Ueli
  • Plant Physiology, Vol. 133, Issue 2, p. 462-469
  • DOI: 10.1104/pp.103.027979

Changes in composition and sugar release across the annual rings of Populus wood and implications on recalcitrance
journal, January 2011


Small-scale and automatable high-throughput compositional analysis of biomass
journal, October 2010

  • DeMartini, Jaclyn D.; Studer, Michael H.; Wyman, Charles E.
  • Biotechnology and Bioengineering, Vol. 108, Issue 2, p. 306-312
  • DOI: 10.1002/bit.22937

An associative analysis of gene expression array data
journal, January 2003


Colorimetric Method for Determination of Sugars and Related Substances
journal, March 1956

  • DuBois, Michel.; Gilles, K. A.; Hamilton, J. K.
  • Analytical Chemistry, Vol. 28, Issue 3, p. 350-356
  • DOI: 10.1021/ac60111a017

Downregulation of Cinnamyl Alcohol Dehydrogenase (CAD) Leads to Improved Saccharification Efficiency in Switchgrass
journal, January 2011


Genetic manipulation of lignin reduces recalcitrance and improves ethanol production from switchgrass
journal, February 2011

  • Fu, Chunxiang; Mielenz, Jonathan R.; Xiao, Xirong
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 9, p. 3803-3808
  • DOI: 10.1073/pnas.1100310108

Ethanol Production from Renewable Resources
book, January 1999

  • Gong, C. S.; Cao, N. J.; Du, J.
  • Recent Progress in Bioconversion of Lignocellulosics, p. 207-241
  • DOI: 10.1007/3-540-49194-5_9

Using the Acetyl Bromide Assay To Determine Lignin Concentrations in Herbaceous Plants:� Some Cautionary Notes
journal, February 1999

  • Hatfield, R. D.; Grabber, J.; Ralph, J.
  • Journal of Agricultural and Food Chemistry, Vol. 47, Issue 2, p. 628-632
  • DOI: 10.1021/jf9808776

Exploration, normalization, and summaries of high density oligonucleotide array probe level data
journal, April 2003


Cellulase digestibility of pretreated biomass is limited by cellulose accessibility
journal, January 2007

  • Jeoh, Tina; Ishizawa, Claudia I.; Davis, Mark F.
  • Biotechnology and Bioengineering, Vol. 98, Issue 1, p. 112-122
  • DOI: 10.1002/bit.21408

New insights into the molecular architecture of hardwood lignins by chemical degradative methods
journal, March 1995

  • Lapierre, Catherine; Pollet, Brigitte; Rolando, Christian
  • Research on Chemical Intermediates, Vol. 21, Issue 3-5, p. 397-412
  • DOI: 10.1007/BF03052266

The maize brown midrib4 (bm4) gene encodes a functional folylpolyglutamate synthase
journal, January 2015

  • Li, Li; Hill-Skinner, Sarah; Liu, Sanzhen
  • The Plant Journal, Vol. 81, Issue 3, p. 493-504
  • DOI: 10.1111/tpj.12745

Model-based analysis of oligonucleotide arrays: Expression index computation and outlier detection
journal, January 2001

  • Li, C.; Wong, W. H.
  • Proceedings of the National Academy of Sciences, Vol. 98, Issue 1, p. 31-36
  • DOI: 10.1073/pnas.98.1.31

Fermentation of lignocellulosic hydrolysates for ethanol production
journal, April 1996

  • Olsson, Lisbeth; Hahn-H�gerdal, B�rbel
  • Enzyme and Microbial Technology, Vol. 18, Issue 5, p. 312-331
  • DOI: 10.1016/0141-0229(95)00157-3

Immunological Approaches to Plant Cell Wall and Biomass Characterization: Glycome Profiling
book, January 2012


A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies
journal, April 2010

  • Pattathil, S.; Avci, U.; Baldwin, D.
  • Plant Physiology, Vol. 153, Issue 2, p. 514-525
  • DOI: 10.1104/pp.109.151985

Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data
journal, March 2003

  • Ramakers, Christian; Ruijter, Jan M.; Deprez, Ronald H. Lekanne
  • Neuroscience Letters, Vol. 339, Issue 1, p. 62-66
  • DOI: 10.1016/S0304-3940(02)01423-4

Methionine catabolism in Arabidopsis cells is initiated by a ?-cleavage process and leads to S-methylcysteine and isoleucine syntheses
journal, October 2006

  • Rebeille, F.; Jabrin, S.; Bligny, R.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 42, p. 15687-15692
  • DOI: 10.1073/pnas.0606195103

Functional characterization of the switchgrass (Panicum virgatum) R2R3-MYB transcription factor PvMYB4 for improvement of lignocellulosic feedstocks
journal, October 2011


Collection and Analysis of Expressed Sequence Tags Derived from Laser Capture Microdissected Switchgrass (Panicum virgatum L. Alamo) Vascular Tissues
journal, February 2010

  • Srivastava, Avinash C.; Palanichelvam, Karuppaiah; Ma, Junying
  • BioEnergy Research, Vol. 3, Issue 3, p. 278-294
  • DOI: 10.1007/s12155-010-9080-8

The Folylpolyglutamate Synthetase Plastidial Isoform Is Required for Postembryonic Root Development in Arabidopsis
journal, January 2011

  • Srivastava, A. C.; Ramos-Parra, P. A.; Bedair, M.
  • Plant Physiology, Vol. 155, Issue 3, p. 1237-1251
  • DOI: 10.1104/pp.110.168278

The plastidial folylpolyglutamate synthetase and root apical meristem maintenance
journal, May 2011

  • Srivastava, Avinash C.; Tang, Yuhong; D�az de la Garza, Roc�o I.
  • Plant Signaling & Behavior, Vol. 6, Issue 5, p. 751-754
  • DOI: 10.4161/psb.6.5.15403

Loss of function of folylpolyglutamate synthetase 1 reduces lignin content and improves cell wall digestibility in Arabidopsis
journal, December 2015

  • Srivastava, Avinash C.; Chen, Fang; Ray, Tui
  • Biotechnology for Biofuels, Vol. 8, Article No. 224
  • DOI: 10.1186/s13068-015-0403-z

Engineering of a high-throughput screening system to identify cellulosic biomass, pretreatments, and enzyme formulations that enhance sugar release
journal, February 2010

  • Studer, Michael H.; DeMartini, Jaclyn D.; Brethauer, Simone
  • Biotechnology and Bioengineering, Vol. 105, Issue 2, p. 231-238
  • DOI: 10.1002/bit.22527

Hydrolysis of lignocellulosic materials for ethanol production: a review
journal, May 2002


Vascular Patterning
journal, January 2003

  • Turner, Simon; Sieburth, Leslie E.
  • The Arabidopsis Book, Vol. 2, Article No. e0073
  • DOI: 10.1199/tab.0073

Lignin biosynthesis perturbations affect secondary cell wall composition and saccharification yield in Arabidopsis thaliana
journal, January 2013

  • Van Acker, Rebecca; Vanholme, Ruben; Storme, V�ronique
  • Biotechnology for Biofuels, Vol. 6, Article No. 46
  • DOI: 10.1186/1754-6834-6-46

Biomass Ethanol: Technical Progress, Opportunities, and Commercial Challenges
journal, November 1999


Virus-Induced Gene Silencing Offers a Functional Genomics Platform for Studying Plant Cell Wall Formation
journal, September 2010

  • Zhu, Xiaohong; Pattathil, Sivakumar; Mazumder, Koushik
  • Molecular Plant, Vol. 3, Issue 5, p. 818-833
  • DOI: 10.1093/mp/ssq023