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Title: Enhanced itaconic acid production in Aspergillus with increased LaeA expression

Abstract

Fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (LaeA), or both, are described. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also described, as are compositions and kits including the disclosed fungi. Further described are Aspergillus terreus fungi overexpressing the LaeA gene and the use of such fungi for the production of itaconic acid.

Inventors:
;
Issue Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1425892
Patent Number(s):
9909152
Application Number:
14/928,511
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015 Oct 30
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Dai, Ziyu, and Baker, Scott E. Enhanced itaconic acid production in Aspergillus with increased LaeA expression. United States: N. p., 2018. Web.
Dai, Ziyu, & Baker, Scott E. Enhanced itaconic acid production in Aspergillus with increased LaeA expression. United States.
Dai, Ziyu, and Baker, Scott E. Tue . "Enhanced itaconic acid production in Aspergillus with increased LaeA expression". United States. https://www.osti.gov/servlets/purl/1425892.
@article{osti_1425892,
title = {Enhanced itaconic acid production in Aspergillus with increased LaeA expression},
author = {Dai, Ziyu and Baker, Scott E.},
abstractNote = {Fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (LaeA), or both, are described. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also described, as are compositions and kits including the disclosed fungi. Further described are Aspergillus terreus fungi overexpressing the LaeA gene and the use of such fungi for the production of itaconic acid.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {3}
}

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Works referenced in this record:

LaeA, a global regulator of Aspergillus toxins
journal, January 2006


Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine
journal, September 1999


Effective lead selection for improved protein production in Aspergillus niger based on integrated genomics
journal, March 2009


The effects of culture conditions on the glycosylation of secreted human placental alkaline phosphatase produced in Chinese hamster ovary cells
journal, August 2008


Overexpression of the Aspergillus nidulans histone 4 acetyltransferase EsaA increases activation of secondary metabolite production
journal, August 2012


Molecular cloning and characterization of the global regulator LaeA in Penicillium citrinum
journal, August 2010


CDG-Id caused by homozygosity for an ALG3 mutation due to segmental maternal isodisomy UPD3(q21.3-qter)
journal, April 2005


Assembly of Asparagine-Linked Oligosaccharides
journal, June 1985


Aspergillus oryzae laeA Regulates Kojic Acid Synthesis Genes
journal, September 2011


Requirement of LaeA for secondary metabolism and sclerotial production in Aspergillus flavus
journal, October 2008


Arabidopsis thaliana ALG3 mutant synthesizes immature oligosaccharides in the ER and accumulates unique N-glycans
journal, March 2010


Volatile profiling reveals intracellular metabolic changes in Aspergillus parasiticus: veA regulates branched chain amino acid and ethanol metabolism
journal, January 2010


Biochemistry, molecular biology, and genetics of the oligosaccharyltransferase.
journal, June 1996


Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88
journal, January 2007


Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015 versus enzyme-producing CBS 513.88
journal, May 2011


Genomics of protein folding in the endoplasmic reticulum, secretion stress and glycosylation in the aspergilli
journal, March 2009


Role of Glycosylation in Development
journal, June 2004


Chromatin-level regulation of biosynthetic gene clusters
journal, May 2009


A plant DNA minipreparation: Version II
journal, September 1983


Carbohydrate deficient glycoprotein syndrome type IV: deficiency of dolichyl‐P‐Man:Man5 GlcNAc2‐PP‐dolichyl mannosyltransferase
journal, December 1999


VelB/VeA/LaeA Complex Coordinates Light Signal with Fungal Development and Secondary Metabolism
journal, June 2008


The sequence–structure relationship and protein function prediction
journal, June 2009


Accurate prediction of the Aspergillus nidulans terrequinone gene cluster boundaries using the transcriptional regulator LaeA
journal, November 2007


Transformation of Aspergillus sp. and Trichoderma reesei Using the Pyrithiamine Resistance Gene (ptrA) of Aspergillus oryzae
journal, January 2002


Protein glycosylation pathways in filamentous fungi
journal, May 2008


On the safety of Aspergillus niger - a review
journal, January 2002


Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different
journal, April 2001


Functional analysis of the ALG3 gene encoding the Dol-P-Man: Man5GlcNAc2-PP-Dol mannosyltransferase enzyme of P. pastoris
journal, January 2004


Initiation of translation in prokaryotes and eukaryotes
journal, July 1999


Analytical and computational approaches to define the Aspergillus niger secretome
journal, March 2009


Proteomics of industrial fungi: trends and insights for biotechnology
journal, October 2010


LaeA, a Regulator of Secondary Metabolism in Aspergillus spp.
journal, April 2004


N-Glycan Modification in Aspergillus Species
journal, December 2007


Carbohydrate-Deficient Glycoprotein Syndrome - A Fourth Subtype
journal, October 1995


Heterologous expression system in Aspergillus oryzae for fungal biosynthetic gene clusters of secondary metabolites
journal, November 2011


Human Sperm Binding Is Mediated by the Sialyl-Lewisx Oligosaccharide on the Zona Pellucida
journal, August 2011


Congenital Disorder of Glycosylation Id Presenting with Hyperinsulinemic Hypoglycemia and Islet Cell Hyperplasia
journal, July 2005


Lae1 regulates expression of multiple secondary metabolite gene clusters in Fusarium verticillioides
journal, August 2012


Secondary metabolic gene cluster silencing in Aspergillus nidulans
journal, September 2006


CDG-Id in two siblings with partially different phenotypes
journal, January 2007


Organic Acid Production by Filamentous Fungi
book, January 2004


Unraveling the Mechanism of Protein N-Glycosylation
journal, December 2004


Global analysis of gene transcription regulation in prokaryotes
journal, August 2006


Microbial production of organic acids: expanding the markets
journal, February 2008


Improving itaconic acid production through genetic engineering of an industrial Aspergillus terreus strain
journal, August 2014


Deletion of the TbALG3 gene demonstrates site-specific N-glycosylation and N-glycan processing in Trypanosoma brucei
journal, February 2008


Beyond aflatoxin: four distinct expression patterns and functional roles associated with Aspergillus flavus secondary metabolism gene clusters
journal, March 2010


Heterochromatic marks are associated with the repression of secondary metabolism clusters in Aspergillus nidulans
journal, February 2010


LaeA Control of Velvet Family Regulatory Proteins for Light-Dependent Development and Fungal Cell-Type Specificity
journal, December 2010


Conservation of structure and function of the aflatoxin regulatory geneaflR fromAspergillus nidulans andA. flavus
journal, May 1996


Quality Control and Protein Folding in the Secretory Pathway
journal, November 2003


Centralized Modularity of N-Linked Glycosylation Pathways in Mammalian Cells
journal, October 2009


Molecular cloning and enzymatic characterization of a Trichoderma reesei 1,2-α-d-mannosidase
journal, February 2000


Heterologous protein expression in filamentous fungi
journal, September 2005


Congenital Disorder of Glycosylation Type Id: Clinical Phenotype, Molecular Analysis, Prenatal Diagnosis, and Glycosylation of Fetal Proteins
journal, August 2005


The VeA regulatory system and its role in morphological and chemical development in fungi
journal, July 2008


Cloning and characterization of the ALG3 gene of Saccharomyces cerevisiae
journal, January 1996


Identification and characterization of Penicillium citrinum VeA and LaeA as global regulators for ML-236B production
journal, December 2011


Advances in the production of human therapeutic proteins in yeasts and filamentous fungi
journal, November 2004


Protein Glycosylation in Yeast
journal, June 1987


Filamentous fungi as cell factories for heterologous protein production
journal, May 2002


Approaching the Secrets of N-Glycosylation in Aspergillus fumigatus: Characterization of the AfOch1 Protein
journal, December 2010


On the frequency of protein glycosylation, as deduced from analysis of the SWISS-PROT database
journal, December 1999