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Title: Enhanced itaconic acid production in Aspergillus with increased LaeA expression

Abstract

Fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (LaeA), or both, are described. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also described, as are compositions and kits including the disclosed fungi. Further described are Aspergillus terreus fungi overexpressing the LaeA gene and the use of such fungi for the production of itaconic acid.

Inventors:
;
Issue Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1425892
Patent Number(s):
9909152
Application Number:
14/928,511
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015 Oct 30
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Dai, Ziyu, and Baker, Scott E. Enhanced itaconic acid production in Aspergillus with increased LaeA expression. United States: N. p., 2018. Web.
Dai, Ziyu, & Baker, Scott E. Enhanced itaconic acid production in Aspergillus with increased LaeA expression. United States.
Dai, Ziyu, and Baker, Scott E. Tue . "Enhanced itaconic acid production in Aspergillus with increased LaeA expression". United States. https://www.osti.gov/servlets/purl/1425892.
@article{osti_1425892,
title = {Enhanced itaconic acid production in Aspergillus with increased LaeA expression},
author = {Dai, Ziyu and Baker, Scott E.},
abstractNote = {Fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (LaeA), or both, are described. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also described, as are compositions and kits including the disclosed fungi. Further described are Aspergillus terreus fungi overexpressing the LaeA gene and the use of such fungi for the production of itaconic acid.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {3}
}

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