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Title: Growth arrested cells useful for producing compounds


The present invention provides for a genetically modified host cell in a growth-arrested state producing an antibiotic.

Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
OSTI Identifier:
Patent Number(s):
Application Number:
The Regents of the University of California (Oakland, CA)
DOE Contract Number:  
Resource Type:
Resource Relation:
Patent File Date: 2015 May 11
Country of Publication:
United States

Citation Formats

Bokinsky, Gregory E., and Keasling, Jay D. Growth arrested cells useful for producing compounds. United States: N. p., 2018. Web.
Bokinsky, Gregory E., & Keasling, Jay D. Growth arrested cells useful for producing compounds. United States.
Bokinsky, Gregory E., and Keasling, Jay D. Tue . "Growth arrested cells useful for producing compounds". United States.
title = {Growth arrested cells useful for producing compounds},
author = {Bokinsky, Gregory E. and Keasling, Jay D.},
abstractNote = {The present invention provides for a genetically modified host cell in a growth-arrested state producing an antibiotic.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {2}


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Works referenced in this record:

Bacterial persistence by RNA endonucleases
journal, July 2011

  • Maisonneuve, Etienne; Shakespeare, Lana J.; Jørgensen, Mikkel Girke
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 32, p. 13206-13211
  • DOI: 10.1073/pnas.1100186108

The Stringent Response and Cell Cycle Arrest in Escherichia coli
journal, December 2008

  • Ferullo, Daniel J.; Lovett, Susan T.; Burkholder, William F.
  • PLoS Genetics, Vol. 4, Issue 12, Article No. e1000300
  • DOI: 10.1371/journal.pgen.1000300

Kinase Activity of Overexpressed HipA Is Required for Growth Arrest and Multidrug Tolerance in Escherichia coli
journal, October 2006

  • Correia, F. F.; D'Onofrio, A.; Rejtar, T.
  • Journal of Bacteriology, Vol. 188, Issue 24, p. 8360-8367
  • DOI: 10.1128/JB.01237-06

Persister Cells
journal, October 2010

ppGpp is the major source of growth rate control in E. coli
journal, October 2010

Direct binding targets of the stringent response alarmone (p)ppGpp
journal, August 2012

HipA-Triggered Growth Arrest and β-Lactam Tolerance in Escherichia coli Are Mediated by RelA-Dependent ppGpp Synthesis
journal, May 2013

  • Bokinsky, G.; Baidoo, E. E. K.; Akella, S.
  • Journal of Bacteriology, Vol. 195, Issue 14, p. 3173-3182
  • DOI: 10.1128/JB.02210-12

Synthesis of Guanosine Tetra- and Pentaphosphate Requires the Presence of a Codon-Specific, Uncharged Transfer Ribonucleic Acid in the Acceptor Site of Ribosomes
journal, May 1973

  • Haseltine, W. A.; Block, R.
  • Proceedings of the National Academy of Sciences, Vol. 70, Issue 5, p. 1564-1568
  • DOI: 10.1073/pnas.70.5.1564

Direct correlation between overproduction of guanosine 3',5'-bispyrophosphate (ppGpp) and penicillin tolerance in Escherichia coli.
journal, August 1995

Molecular Mechanisms of HipA-Mediated Multidrug Tolerance and Its Neutralization by HipB
journal, January 2009

Regulation of growth and death in Escherichia coli by toxin–antitoxin systems
journal, September 2011

  • Yamaguchi, Yoshihiro; Inouye, Masayori
  • Nature Reviews Microbiology, Vol. 9, Issue 11, p. 779-790
  • DOI: 10.1038/nrmicro2651

ppGpp inhibits the activity of Escherichia coli DnaG primase
journal, January 2010

Transcriptional cross-activation between toxin-antitoxin systems of Escherichia coli
journal, January 2013

  • Kasari, Villu; Mets, Toomas; Tenson, Tanel
  • BMC Microbiology, Vol. 13, Issue 1, Article No. 45
  • DOI: 10.1186/1471-2180-13-45

Nutritional Control of Elongation of DNA Replication by (p)ppGpp
journal, March 2007

DksA Affects ppGpp Induction of RpoS at a Translational Level
journal, August 2002

Ectopic Overexpression of Wild-Type and Mutant hipA Genes in Escherichia coli: Effects on Macromolecular Synthesis and Persister Formation
journal, May 2006

  • Korch, S. B.; Hill, T. M.
  • Journal of Bacteriology, Vol. 188, Issue 11, p. 3826-3836
  • DOI: 10.1128/JB.01740-05

Persister cells and tolerance to antimicrobials
journal, January 2004

Increased Persistence in Escherichia coli Caused by Controlled Expression of Toxins or Other Unrelated Proteins
journal, May 2006

Delayed-relaxed response explained by hyperactivation of RelE
journal, June 2004

Characterization of the hipA7 allele of Escherichia coli and evidence that high persistence is governed by (p)ppGpp synthesis: Persistence and (p)ppGpp synthesis in E. coli
journal, November 2003

Manipulation of the carbon storage regulator system for metabolite remodeling and biofuel production in Escherichia coli
journal, January 2012

  • McKee, Adrienne E.; Rutherford, Becky J.; Chivian, Dylan C.
  • Microbial Cell Factories, Vol. 11, Issue 1, Article No. 79
  • DOI: 10.1186/1475-2859-11-79

Phosphorylation of Elongation Factor Tu Prevents Ternary Complex Formation
journal, June 1995

  • Alexander, Christian; Bilgin, Nese; Lindschau, Carsten
  • Journal of Biological Chemistry, Vol. 270, Issue 24
  • DOI: 10.1074/jbc.270.24.14541

A comprehensive library of fluorescent transcriptional reporters for Escherichia coli
journal, August 2006

  • Zaslaver, Alon; Bren, Anat; Ronen, Michal
  • Nature Methods, Vol. 3, Issue 8, p. 623-628
  • DOI: 10.1038/nmeth895

Heterogeneous bacterial persisters and engineering approaches to eliminate them
journal, October 2011

  • Allison, Kyle R.; Brynildsen, Mark P.; Collins, James J.
  • Current Opinion in Microbiology, Vol. 14, Issue 5, p. 593-598
  • DOI: 10.1016/j.mib.2011.09.002

Active Starvation Responses Mediate Antibiotic Tolerance in Biofilms and Nutrient-Limited Bacteria
journal, November 2011

Bacterial Persistence and Toxin-Antitoxin Loci
journal, October 2012

Bacterial Persistence as a Phenotypic Switch
journal, September 2004

  • Balaban, Nathalie Q.; Merrin, Jack; Chait, Remy
  • Science, Vol. 305, Issue 5690, p. 1622-1625
  • DOI: 10.1126/science.1099390