Growth arrested cells useful for producing compounds
Abstract
The present invention provides for a genetically modified host cell in a growth-arrested state producing an antibiotic.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1423449
- Patent Number(s):
- 9902964
- Application Number:
- 14/709,328
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC02-05CH11231
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2015 May 11
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
Bokinsky, Gregory E., and Keasling, Jay D. Growth arrested cells useful for producing compounds. United States: N. p., 2018.
Web.
Bokinsky, Gregory E., & Keasling, Jay D. Growth arrested cells useful for producing compounds. United States.
Bokinsky, Gregory E., and Keasling, Jay D. Tue .
"Growth arrested cells useful for producing compounds". United States. https://www.osti.gov/servlets/purl/1423449.
@article{osti_1423449,
title = {Growth arrested cells useful for producing compounds},
author = {Bokinsky, Gregory E. and Keasling, Jay D.},
abstractNote = {The present invention provides for a genetically modified host cell in a growth-arrested state producing an antibiotic.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {2}
}
Works referenced in this record:
Bacterial persistence by RNA endonucleases
journal, July 2011
- Maisonneuve, Etienne; Shakespeare, Lana J.; Jørgensen, Mikkel Girke
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 32, p. 13206-13211
The Stringent Response and Cell Cycle Arrest in Escherichia coli
journal, December 2008
- Ferullo, Daniel J.; Lovett, Susan T.; Burkholder, William F.
- PLoS Genetics, Vol. 4, Issue 12, Article No. e1000300
Kinase Activity of Overexpressed HipA Is Required for Growth Arrest and Multidrug Tolerance in Escherichia coli
journal, October 2006
- Correia, F. F.; D'Onofrio, A.; Rejtar, T.
- Journal of Bacteriology, Vol. 188, Issue 24, p. 8360-8367
Persister Cells
journal, October 2010
- Lewis, Kim
- Annual Review of Microbiology, Vol. 64, Issue 1, p. 357-372
ppGpp is the major source of growth rate control in E. coli
journal, October 2010
- Potrykus, Katarzyna; Murphy, Helen; Philippe, Nadège
- Environmental Microbiology, Vol. 13, Issue 3, p. 563-575
Direct binding targets of the stringent response alarmone (p)ppGpp
journal, August 2012
- Kanjee, Usheer; Ogata, Koji; Houry, Walid A.
- Molecular Microbiology, Vol. 85, Issue 6, p. 1029-1043
HipA-Triggered Growth Arrest and β-Lactam Tolerance in Escherichia coli Are Mediated by RelA-Dependent ppGpp Synthesis
journal, May 2013
- Bokinsky, G.; Baidoo, E. E. K.; Akella, S.
- Journal of Bacteriology, Vol. 195, Issue 14, p. 3173-3182
Synthesis of Guanosine Tetra- and Pentaphosphate Requires the Presence of a Codon-Specific, Uncharged Transfer Ribonucleic Acid in the Acceptor Site of Ribosomes
journal, May 1973
- Haseltine, W. A.; Block, R.
- Proceedings of the National Academy of Sciences, Vol. 70, Issue 5, p. 1564-1568
Direct correlation between overproduction of guanosine 3',5'-bispyrophosphate (ppGpp) and penicillin tolerance in Escherichia coli.
journal, August 1995
- Rodionov, D. G.; Ishiguro, E. E.
- Journal of Bacteriology, Vol. 177, Issue 15, p. 4224-4229
Metabolite-enabled eradication of bacterial persisters by aminoglycosides
journal, May 2011
- Allison, Kyle R.; Brynildsen, Mark P.; Collins, James J.
- Nature, Vol. 473, Issue 7346
Molecular Mechanisms of HipA-Mediated Multidrug Tolerance and Its Neutralization by HipB
journal, January 2009
- Schumacher, M. A.; Piro, K. M.; Xu, W.
- Science, Vol. 323, Issue 5912, p. 396-401
hipA, a newly recognized gene of Escherichia coli K-12 that affects frequency of persistence after inhibition of murein synthesis.
journal, January 1983
- Moyed, H. S.; Bertrand, K. P.
- Journal of Bacteriology, Vol. 155, Issue 2
Regulation of growth and death in Escherichia coli by toxin–antitoxin systems
journal, September 2011
- Yamaguchi, Yoshihiro; Inouye, Masayori
- Nature Reviews Microbiology, Vol. 9, Issue 11, p. 779-790
ppGpp inhibits the activity of Escherichia coli DnaG primase
journal, January 2010
- Maciąg, Monika; Kochanowska, Maja; Łyżeń, Robert
- Plasmid, Vol. 63, Issue 1, p. 61-67
Transcriptional cross-activation between toxin-antitoxin systems of Escherichia coli
journal, January 2013
- Kasari, Villu; Mets, Toomas; Tenson, Tanel
- BMC Microbiology, Vol. 13, Issue 1, Article No. 45
Nutritional Control of Elongation of DNA Replication by (p)ppGpp
journal, March 2007
- Wang, Jue D.; Sanders, Glenn M.; Grossman, Alan D.
- Cell, Vol. 128, Issue 5, p. 865-875
DksA Affects ppGpp Induction of RpoS at a Translational Level
journal, August 2002
- Brown, L.; Gentry, D.; Elliott, T.
- Journal of Bacteriology, Vol. 184, Issue 16, p. 4455-4465
Ectopic Overexpression of Wild-Type and Mutant hipA Genes in Escherichia coli: Effects on Macromolecular Synthesis and Persister Formation
journal, May 2006
- Korch, S. B.; Hill, T. M.
- Journal of Bacteriology, Vol. 188, Issue 11, p. 3826-3836
Persister cells and tolerance to antimicrobials
journal, January 2004
- Keren, Iris; Kaldalu, Niilo; Spoering, Amy
- FEMS Microbiology Letters, Vol. 230, Issue 1
Gene cluster for thienamycin biosynthesis, genetic manipulation and utility
patent-application, February 2004
- Nunez, Luz Elena; Blanco, Gloria; Brana, Alfredo
- US Patent Application 10/374903; 20040038250
Increased Persistence in Escherichia coli Caused by Controlled Expression of Toxins or Other Unrelated Proteins
journal, May 2006
- Vazquez-Laslop, N.; Lee, H.; Neyfakh, A. A.
- Journal of Bacteriology, Vol. 188, Issue 10, p. 3494-3497
Delayed-relaxed response explained by hyperactivation of RelE
journal, June 2004
- Christensen, Susanne K.; Gerdes, Kenn
- Molecular Microbiology, Vol. 53, Issue 2, p. 587-597
Characterization of the hipA7 allele of Escherichia coli and evidence that high persistence is governed by (p)ppGpp synthesis: Persistence and (p)ppGpp synthesis in E. coli
journal, November 2003
- Korch, Shaleen B.; Henderson, Thomas A.; Hill, Thomas M.
- Molecular Microbiology, Vol. 50, Issue 4, p. 1199-1213
Molecular cloning and expression of hipA, a gene of Escherichia coli K-12 that affects frequency of persistence after inhibition of murein synthesis.
journal, May 1986
- Moyed, H. S.; Broderick, S. H.
- Journal of Bacteriology, Vol. 166, Issue 2, p. 399-403
Genes involved in the biosynthetic pathway of carbapenem
patent, February 1999
- Salmond, George Peacock Copeland; McGowan, Simon James; Sebaihia, Mohammed
- US Patent Document 5,871,922
Manipulation of the carbon storage regulator system for metabolite remodeling and biofuel production in Escherichia coli
journal, January 2012
- McKee, Adrienne E.; Rutherford, Becky J.; Chivian, Dylan C.
- Microbial Cell Factories, Vol. 11, Issue 1, Article No. 79
Phosphorylation of Elongation Factor Tu Prevents Ternary Complex Formation
journal, June 1995
- Alexander, Christian; Bilgin, Nese; Lindschau, Carsten
- Journal of Biological Chemistry, Vol. 270, Issue 24
A comprehensive library of fluorescent transcriptional reporters for Escherichia coli
journal, August 2006
- Zaslaver, Alon; Bren, Anat; Ronen, Michal
- Nature Methods, Vol. 3, Issue 8, p. 623-628
Heterogeneous bacterial persisters and engineering approaches to eliminate them
journal, October 2011
- Allison, Kyle R.; Brynildsen, Mark P.; Collins, James J.
- Current Opinion in Microbiology, Vol. 14, Issue 5, p. 593-598
Active Starvation Responses Mediate Antibiotic Tolerance in Biofilms and Nutrient-Limited Bacteria
journal, November 2011
- Nguyen, D.; Joshi-Datar, A.; Lepine, F.
- Science, Vol. 334, Issue 6058, p. 982-986
Bacterial Persistence and Toxin-Antitoxin Loci
journal, October 2012
- Gerdes, Kenn; Maisonneuve, Etienne
- Annual Review of Microbiology, Vol. 66, Issue 1, p. 103-123
Bacterial Persistence as a Phenotypic Switch
journal, September 2004
- Balaban, Nathalie Q.; Merrin, Jack; Chait, Remy
- Science, Vol. 305, Issue 5690, p. 1622-1625