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Title: Quantitative detection of pathogens in centrifugal microfluidic disks

Abstract

A system and methods for detection of a nucleic acid including forming a plurality of nucleic acid detection complexes are described, each of the complexes including a nucleic acid analyte, a detection agent and a functionalized probe. The method further including binding the nucleic acid detection complexes to a plurality of functionalized particles in a fluid sample and separating the functionalized particles having the nucleic acid detection complexes bound thereto from the fluid sample using a density media. The nucleic acid analyte is detected by detecting the detection agent.

Inventors:
; ;
Issue Date:
Research Org.:
Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1423444
Patent Number(s):
9903001
Application Number:
13/941,186
Assignee:
National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 Jul 12
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Koh, Chung-Yan, Schaff, Ulrich Y., and Sommer, Gregory Jon. Quantitative detection of pathogens in centrifugal microfluidic disks. United States: N. p., 2018. Web.
Koh, Chung-Yan, Schaff, Ulrich Y., & Sommer, Gregory Jon. Quantitative detection of pathogens in centrifugal microfluidic disks. United States.
Koh, Chung-Yan, Schaff, Ulrich Y., and Sommer, Gregory Jon. Tue . "Quantitative detection of pathogens in centrifugal microfluidic disks". United States. https://www.osti.gov/servlets/purl/1423444.
@article{osti_1423444,
title = {Quantitative detection of pathogens in centrifugal microfluidic disks},
author = {Koh, Chung-Yan and Schaff, Ulrich Y. and Sommer, Gregory Jon},
abstractNote = {A system and methods for detection of a nucleic acid including forming a plurality of nucleic acid detection complexes are described, each of the complexes including a nucleic acid analyte, a detection agent and a functionalized probe. The method further including binding the nucleic acid detection complexes to a plurality of functionalized particles in a fluid sample and separating the functionalized particles having the nucleic acid detection complexes bound thereto from the fluid sample using a density media. The nucleic acid analyte is detected by detecting the detection agent.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {2}
}

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