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Title: Xylanases, nucleic acids encoding them and methods for making and using them

The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal .beta.-1,4-xylosidic linkages or endo-.beta.-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.
Issue Date:
OSTI Identifier:
BP Corporation North America Inc. (Houston, TX) DOEEE
Patent Number(s):
Application Number:
Contract Number:
Resource Relation:
Patent File Date: 2015 Jun 05
Research Org:
USDOE Office of Energy Efficiency and Renewable Energy (EE) (United States)
Sponsoring Org:
Country of Publication:
United States

Works referenced in this record:

Using molecular techniques to identify new microbial biocatalysts
journal, June 1998

Stabilization of xylanase by random mutagenesis
journal, January 1993

Crystallographic and mutational analyses of an extremely acidophilic and acid-stable xylanase: biased distribution of acidic residues and importance of Asp37 for catalysis at low pH
journal, December 1998
  • Fushinobu, S.; Ito, K.; Konno, M.
  • Protein Engineering Design and Selection, Vol. 11, Issue 12, p. 1121-1128
  • DOI: 10.1093/protein/11.12.1121