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Title: Chimeric OspA genes, proteins and methods of use thereof

Abstract

The invention relates to the development of chimeric OspA molecules for use in a new Lyme vaccine. More specifically, the chimeric OspA molecules comprise the proximal portion from one OspA serotype, together with the distal portion from another OspA serotype, while retaining antigenic properties of both of the parent polypeptides. The chimeric OspA molecules are delivered alone or in combination to provide protection against a variety of Borrelia genospecies. The invention also provides methods for administering the chimeric OspA molecules to a subject in the prevention and treatment of Lyme disease or borreliosis.

Inventors:
; ; ; ; ;
Issue Date:
Research Org.:
Research Foundation of the State University of New York, Amherst, NY; Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1422826
Patent Number(s):
9895434
Application Number:
15/049,438
Assignee:
Brookhaven Science Associates, LLC (Upton, NY)
Patent Classifications (CPCs):
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
DOE Contract Number:  
DE-SC0012704
Resource Type:
Patent
Resource Relation:
Patent File Date: 2016 Feb 22
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Crowe, Brian A., Livey, Ian, O'Rourke, Maria, Schwendinger, Michael, Dunn, John J., and Luft, Benjamin J. Chimeric OspA genes, proteins and methods of use thereof. United States: N. p., 2018. Web.
Crowe, Brian A., Livey, Ian, O'Rourke, Maria, Schwendinger, Michael, Dunn, John J., & Luft, Benjamin J. Chimeric OspA genes, proteins and methods of use thereof. United States.
Crowe, Brian A., Livey, Ian, O'Rourke, Maria, Schwendinger, Michael, Dunn, John J., and Luft, Benjamin J. Tue . "Chimeric OspA genes, proteins and methods of use thereof". United States. https://www.osti.gov/servlets/purl/1422826.
@article{osti_1422826,
title = {Chimeric OspA genes, proteins and methods of use thereof},
author = {Crowe, Brian A. and Livey, Ian and O'Rourke, Maria and Schwendinger, Michael and Dunn, John J. and Luft, Benjamin J.},
abstractNote = {The invention relates to the development of chimeric OspA molecules for use in a new Lyme vaccine. More specifically, the chimeric OspA molecules comprise the proximal portion from one OspA serotype, together with the distal portion from another OspA serotype, while retaining antigenic properties of both of the parent polypeptides. The chimeric OspA molecules are delivered alone or in combination to provide protection against a variety of Borrelia genospecies. The invention also provides methods for administering the chimeric OspA molecules to a subject in the prevention and treatment of Lyme disease or borreliosis.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {2}
}

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Works referenced in this record:

Gene Synthesis[New Synthetic Methods(77)]
journal, June 1989


Crystal structure of Lyme disease antigen outer surface protein A complexed with an Fab
journal, April 1997


Reshaping human antibodies: grafting an antilysozyme activity
journal, March 1988


Structure-based Design of a Second-generation Lyme Disease Vaccine Based on a C-terminal Fragment of Borrelia burgdorferi OspA
journal, July 2005


[14] Protein biotinylation
book, January 1990


Effects of amino acid sequence changes on antibody-antigen interactions
journal, January 1994


Use of deoxyinosine-containing primers vs degenerate primers for polymerase chain reaction based on ambiguous sequence information
journal, April 1994


Identification of LFA-1 as a Candidate Autoantigen in Treatment-Resistant Lyme Arthritis
journal, July 1998


Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
journal, May 1986


Characterization of diphtheria fusion proteins targeted to the human interleukin-3 receptor
journal, August 2000


Genetic Analysis of Protein Stability and Function
journal, December 1989


Analysis of the Lipidated Recombinant Outer Surface Protein A fromBorrelia burgdorferiby Mass Spectrometry
journal, March 1997


Enhanced Production of Recombinant Mycobacterium tuberculosis Antigens in Escherichia coli by Replacement of Low-Usage Codons
journal, January 2000


Using Codon Usage to Predict Genes Origin: Is the Escherichia coli Outer Membrane a Patchwork of Products from Different Genomes?
journal, January 1997


From genes to protein structure and function: novel applications of computational approaches in the genomic era
journal, January 2000


Antibodies that react with predetermined sites on proteins
journal, February 1983


Prodrugs of Peptides and Proteins for Improved Formulation and Delivery
journal, April 1993


Improved tools for biological sequence comparison.
journal, April 1988


Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
journal, September 1979


Strategies for Delivery of Peptides Utilizing Absorption-Enhancing Agents
journal, December 1996


Role of attached lipid in immunogenicity of Borrelia burgdorferi OspA.
journal, January 1993


An OspA serotyping system for Borrelia burgdorferi based on reactivity with monoclonal antibodies and OspA sequence analysis.
journal, January 1993


Enhanced evolutionary PCR using oligonucleotides with inosine at the 3′-terminus
journal, January 1991


A new technique for the assay of infectivity of human adenovirus 5 DNA
journal, April 1973


Safety and Immunogenicity of a Recombinant Outer Surface Protein A Lyme Vaccine
journal, June 1994


Purification of Borrelia burgdorferi outer surface protein A (OspA) and analysis of antibody binding domains.
journal, January 1994


By-passing immunisation
journal, September 1992


Reshaping human antibodies for therapy
journal, March 1988


Chimeric human antibody molecules: mouse antigen-binding domains with human constant region domains.
journal, November 1984


Approaches toward the Directed Design of a Vaccine against Borrelia burgdorferi
journal, February 2002

  • Luft, Benjamin J.; Dunn, John J.; Lawson, Catherine L.
  • The Journal of Infectious Diseases, Vol. 185, Issue s1, p. S46-S51
  • https://doi.org/10.1086/338463

A Vaccine Consisting of Recombinant Borrelia burgdorferi Outer-Surface Protein A to Prevent Lyme Disease
journal, July 1998


Vaccination against Lyme Disease with Recombinant Borrelia burgdorferi Outer-Surface Lipoprotein A with Adjuvant
journal, July 1998


By-passing immunization: Human antibodies from V-gene libraries displayed on phage
journal, December 1991


Continuous cultures of fused cells secreting antibody of predefined specificity
journal, August 1975


Solid Phase Peptide Synthesis. I. The Synthesis of a Tetrapeptide
journal, July 1963


Basic local alignment search tool
journal, October 1990


Borrelia burgdorferi OspA is an arthropod-specific transmission-blocking Lyme disease vaccine.
journal, January 1996


A general method applicable to the search for similarities in the amino acid sequence of two proteins
journal, March 1970


Comparison of biosequences
journal, December 1981


Induction of CD8+ cytotoxic T cells by immunization with purified HIV-1 envelope protein in ISCOMs
journal, April 1990


Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid.
journal, July 1984


Replacing the complementarity-determining regions in a human antibody with those from a mouse
journal, May 1986


Expression of tetanus toxin fragment C in E.coli : high level expression by removing rare codons
journal, January 1989


Structural identification of a key protective B-cell epitope in lyme disease antigen OspA
journal, October 2000


Abilities of OspA proteins from different seroprotective groups of Borrelia burgdorferi to protect hamsters from infection.
journal, January 1995


Inhibition of Borrelia burgdorferi-Tick Interactions In Vivo by Outer Surface Protein A Antibody
journal, June 2001


A comprehensive set of sequence analysis programs for the VAX
journal, January 1984


Amino acid substitution matrices from protein blocks.
journal, November 1992


Applications and statistics for multiple high-scoring segments in molecular sequences.
journal, June 1993