Chimeric OspA genes, proteins and methods of use thereof
Abstract
The invention relates to the development of chimeric OspA molecules for use in a new Lyme vaccine. More specifically, the chimeric OspA molecules comprise the proximal portion from one OspA serotype, together with the distal portion from another OspA serotype, while retaining antigenic properties of both of the parent polypeptides. The chimeric OspA molecules are delivered alone or in combination to provide protection against a variety of Borrelia genospecies. The invention also provides methods for administering the chimeric OspA molecules to a subject in the prevention and treatment of Lyme disease or borreliosis.
- Inventors:
- Issue Date:
- Research Org.:
- Research Foundation of the State University of New York, Amherst, NY; Brookhaven National Lab. (BNL), Upton, NY (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1422826
- Patent Number(s):
- 9895434
- Application Number:
- 15/049,438
- Assignee:
- Brookhaven Science Associates, LLC (Upton, NY)
- Patent Classifications (CPCs):
-
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- DOE Contract Number:
- DE-SC0012704
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2016 Feb 22
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Crowe, Brian A., Livey, Ian, O'Rourke, Maria, Schwendinger, Michael, Dunn, John J., and Luft, Benjamin J. Chimeric OspA genes, proteins and methods of use thereof. United States: N. p., 2018.
Web.
Crowe, Brian A., Livey, Ian, O'Rourke, Maria, Schwendinger, Michael, Dunn, John J., & Luft, Benjamin J. Chimeric OspA genes, proteins and methods of use thereof. United States.
Crowe, Brian A., Livey, Ian, O'Rourke, Maria, Schwendinger, Michael, Dunn, John J., and Luft, Benjamin J. Tue .
"Chimeric OspA genes, proteins and methods of use thereof". United States. https://www.osti.gov/servlets/purl/1422826.
@article{osti_1422826,
title = {Chimeric OspA genes, proteins and methods of use thereof},
author = {Crowe, Brian A. and Livey, Ian and O'Rourke, Maria and Schwendinger, Michael and Dunn, John J. and Luft, Benjamin J.},
abstractNote = {The invention relates to the development of chimeric OspA molecules for use in a new Lyme vaccine. More specifically, the chimeric OspA molecules comprise the proximal portion from one OspA serotype, together with the distal portion from another OspA serotype, while retaining antigenic properties of both of the parent polypeptides. The chimeric OspA molecules are delivered alone or in combination to provide protection against a variety of Borrelia genospecies. The invention also provides methods for administering the chimeric OspA molecules to a subject in the prevention and treatment of Lyme disease or borreliosis.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {2}
}
Works referenced in this record:
Gene Synthesis[New Synthetic Methods(77)]
journal, June 1989
- Engels, Joachim W.; Uhlmann, Eugen
- Angewandte Chemie International Edition in English, Vol. 28, Issue 6, p. 716-734
Crystal structure of Lyme disease antigen outer surface protein A complexed with an Fab
journal, April 1997
- Li, H.; Dunn, J. J.; Luft, B. J.
- Proceedings of the National Academy of Sciences, Vol. 94, Issue 8, p. 3584-3589
Reshaping human antibodies: grafting an antilysozyme activity
journal, March 1988
- Verhoeyen, M.; Milstein, C.; Winter, G.
- Science, Vol. 239, Issue 4847, p. 1534-1536
Molecular analysis of linear plasmid-encoded major surface proteins, OspA and OspB, of the Lyme disease spirochaete Borrelia burgdorferi
journal, April 1989
- Bergström, S.; Bundoc, V. G.; Barbour, A. G.
- Molecular Microbiology, Vol. 3, Issue 4, p. 479-486
Structure-based Design of a Second-generation Lyme Disease Vaccine Based on a C-terminal Fragment of Borrelia burgdorferi OspA
journal, July 2005
- Koide, Shohei; Yang, Xiaohua; Huang, Xiaolin
- Journal of Molecular Biology, Vol. 350, Issue 2, p. 290-299
[14] Protein biotinylation
book, January 1990
- Bayer, Edward A.; Wilchek, Meir
- Avidin-Biotin Technology, p. 138-160
Effects of amino acid sequence changes on antibody-antigen interactions
journal, January 1994
- Colman, P. M.
- Research in Immunology, Vol. 145, Issue 1, p. 33-36
Use of deoxyinosine-containing primers vs degenerate primers for polymerase chain reaction based on ambiguous sequence information
journal, April 1994
- Rossolini, Gian Maria; Cresti, Stefania; Ingianni, Angela
- Molecular and Cellular Probes, Vol. 8, Issue 2, p. 91-98
Identification of LFA-1 as a Candidate Autoantigen in Treatment-Resistant Lyme Arthritis
journal, July 1998
- Gross, Dawn M.; Forsthuber, Thomas; Tary-Lehmann, Magdalena
- Science, Vol. 281, Issue 5377, p. 703-706
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
journal, May 1986
- Studier, F. William; Moffatt, Barbara A.
- Journal of Molecular Biology, Vol. 189, Issue 1, p. 113-130
Characterization of diphtheria fusion proteins targeted to the human interleukin-3 receptor
journal, August 2000
- Frankel, Arthur E.; Ramage, Jason; Kiser, Melanie
- Protein Engineering, Design and Selection, Vol. 13, Issue 8, p. 575-581
Genetic Analysis of Protein Stability and Function
journal, December 1989
- Pakula, A. A.; Sauer, R. T.
- Annual Review of Genetics, Vol. 23, Issue 1, p. 289-310
Analysis of the Lipidated Recombinant Outer Surface Protein A fromBorrelia burgdorferiby Mass Spectrometry
journal, March 1997
- Bouchon, Bernadette; Klein, Michèle; Bischoff, Rainer
- Analytical Biochemistry, Vol. 246, Issue 1, p. 52-61
Enhanced Production of Recombinant Mycobacterium tuberculosis Antigens in Escherichia coli by Replacement of Low-Usage Codons
journal, January 2000
- Lakey, D. L.; Voladri, R. K. R.; Edwards, K. M.
- Infection and Immunity, Vol. 68, Issue 1, p. 233-238
Using Codon Usage to Predict Genes Origin: Is the Escherichia coli Outer Membrane a Patchwork of Products from Different Genomes?
journal, January 1997
- Guerdoux-Jamet, Pascale; Hénaut, Alain; Nitschké, Patrick
- DNA Research, Vol. 4, Issue 4, p. 257-265
From genes to protein structure and function: novel applications of computational approaches in the genomic era
journal, January 2000
- Skolnick, Jeffrey; Fetrow, Jacquelyn S.
- Trends in Biotechnology, Vol. 18, Issue 1, p. 34-39
Antibodies that react with predetermined sites on proteins
journal, February 1983
- Sutcliffe, J.; Shinnick, T.; Green, N
- Science, Vol. 219, Issue 4585, p. 660-666
Prodrugs of Peptides and Proteins for Improved Formulation and Delivery
journal, April 1993
- Oliyai, Reza; Stella, Valentino J.
- Annual Review of Pharmacology and Toxicology, Vol. 33, Issue 1, p. 521-544
Improved tools for biological sequence comparison.
journal, April 1988
- Pearson, W. R.; Lipman, D. J.
- Proceedings of the National Academy of Sciences, Vol. 85, Issue 8, p. 2444-2448
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
journal, September 1979
- Towbin, H.; Staehelin, T.; Gordon, J.
- Proceedings of the National Academy of Sciences, Vol. 76, Issue 9, p. 4350-4354
Strategies for Delivery of Peptides Utilizing Absorption-Enhancing Agents
journal, December 1996
- Fix, Joseph A.
- Journal of Pharmaceutical Sciences, Vol. 85, Issue 12, p. 1282-1285
Role of attached lipid in immunogenicity of Borrelia burgdorferi OspA.
journal, January 1993
- Erdile, L. F.; Brandt, M. A.; Warakomski, D. J.
- Infection and Immunity, Vol. 61, Issue 1
An OspA serotyping system for Borrelia burgdorferi based on reactivity with monoclonal antibodies and OspA sequence analysis.
journal, January 1993
- Wilske, B.; Preac-Mursic, V.; Göbel, U. B.
- Journal of Clinical Microbiology, Vol. 31, Issue 2
Enhanced evolutionary PCR using oligonucleotides with inosine at the 3′-terminus
journal, January 1991
- Batzer, Mark A.; Carlton, James E.; Deininger, Prescott L.
- Nucleic Acids Research, Vol. 19, Issue 18, p. 5081-5081
A new technique for the assay of infectivity of human adenovirus 5 DNA
journal, April 1973
- Graham, F. L.; van der Eb, A. J.
- Virology, Vol. 52, Issue 2, p. 456-467
Safety and Immunogenicity of a Recombinant Outer Surface Protein A Lyme Vaccine
journal, June 1994
- Keller, David; Koster, Frederick T.; Marks, Donald H.
- JAMA: The Journal of the American Medical Association, Vol. 271, Issue 22, p. 1764-1768
Purification of Borrelia burgdorferi outer surface protein A (OspA) and analysis of antibody binding domains.
journal, January 1994
- Jiang, W.; Gorevic, P. D.; Dattwyler, R. J.
- Clinical and diagnostic laboratory immunology, Vol. 1, Issue 4
Reactivity with a specific epitope of outer surface protein A predicts protection from infection with the Lyme disease spirochete, Borrelia burgdorferi.
journal, January 1997
- Golde, W. T.; Piesman, J.; Dolan, M. C.
- Infection and immunity, Vol. 65, Issue 3
By-passing immunisation
journal, September 1992
- Hoogenboom, Hennie R.; Winter, Greg
- Journal of Molecular Biology, Vol. 227, Issue 2, p. 381-388
Reshaping human antibodies for therapy
journal, March 1988
- Riechmann, Lutz; Clark, Michael; Waldmann, Herman
- Nature, Vol. 332, Issue 6162, p. 323-327
Chimeric human antibody molecules: mouse antigen-binding domains with human constant region domains.
journal, November 1984
- Morrison, S. L.; Johnson, M. J.; Herzenberg, L. A.
- Proceedings of the National Academy of Sciences, Vol. 81, Issue 21, p. 6851-6855
Approaches toward the Directed Design of a Vaccine against Borrelia burgdorferi
journal, February 2002
- Luft, Benjamin J.; Dunn, John J.; Lawson, Catherine L.
- The Journal of Infectious Diseases, Vol. 185, Issue s1, p. S46-S51
A Vaccine Consisting of Recombinant Borrelia burgdorferi Outer-Surface Protein A to Prevent Lyme Disease
journal, July 1998
- Sigal, Leonard H.; Zahradnik, John M.; Lavin, Philip
- New England Journal of Medicine, Vol. 339, Issue 4, p. 216-222
Vaccination against Lyme Disease with Recombinant Borrelia burgdorferi Outer-Surface Lipoprotein A with Adjuvant
journal, July 1998
- Steere, Allen C.; Sikand, Vijay K.; Meurice, François
- New England Journal of Medicine, Vol. 339, Issue 4, p. 209-215
By-passing immunization: Human antibodies from V-gene libraries displayed on phage
journal, December 1991
- Marks, James D.; Hoogenboom, Hennie R.; Bonnert, Timothy P.
- Journal of Molecular Biology, Vol. 222, Issue 3, p. 581-597
Continuous cultures of fused cells secreting antibody of predefined specificity
journal, August 1975
- Köhler, G.; Milstein, C.
- Nature, Vol. 256, Issue 5517, p. 495-497
Solid Phase Peptide Synthesis. I. The Synthesis of a Tetrapeptide
journal, July 1963
- Merrifield, R. B.
- Journal of the American Chemical Society, Vol. 85, Issue 14, p. 2149-2154
Basic local alignment search tool
journal, October 1990
- Altschul, Stephen F.; Gish, Warren; Miller, Webb
- Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
Borrelia burgdorferi OspA is an arthropod-specific transmission-blocking Lyme disease vaccine.
journal, January 1996
- de Silva, A. M.; Telford, S. R.; Brunet, L. R.
- Journal of Experimental Medicine, Vol. 183, Issue 1, p. 271-275
A general method applicable to the search for similarities in the amino acid sequence of two proteins
journal, March 1970
- Needleman, Saul B.; Wunsch, Christian D.
- Journal of Molecular Biology, Vol. 48, Issue 3, p. 443-453
Comparison of biosequences
journal, December 1981
- Smith, Temple F.; Waterman, Michael S.
- Advances in Applied Mathematics, Vol. 2, Issue 4, p. 482-489
Induction of CD8+ cytotoxic T cells by immunization with purified HIV-1 envelope protein in ISCOMs
journal, April 1990
- Takahashi, Hidemi; Takeshfta, Toshiyuki; Morein, Bror
- Nature, Vol. 344, Issue 6269, p. 873-875
The Lyme disease vaccine candidate outer surface protein A (OspA) in a formulation compatible with human use protects mice against natural tick transmission of B. burgdorferi
journal, January 1995
- Golde, William T.; Burkot, Thomas R.; Piesman, Joseph
- Vaccine, Vol. 13, Issue 5, p. 435-441
Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid.
journal, July 1984
- Geysen, H. M.; Meloen, R. H.; Barteling, S. J.
- Proceedings of the National Academy of Sciences, Vol. 81, Issue 13, p. 3998-4002
General method for the rapid solid-phase synthesis of large numbers of peptides: specificity of antigen-antibody interaction at the level of individual amino acids.
journal, August 1985
- Houghten, R. A.
- Proceedings of the National Academy of Sciences, Vol. 82, Issue 15, p. 5131-5135
Organization of genes encoding two outer membrane proteins of the Lyme disease agent Borrelia burgdorferi within a single transcriptional unit.
journal, January 1986
- Howe, T. R.; LaQuier, F. W.; Barbour, A. G.
- Infection and Immunity, Vol. 54, Issue 1
Replacing the complementarity-determining regions in a human antibody with those from a mouse
journal, May 1986
- Jones, Peter T.; Dear, Paul H.; Foote, Jefferson
- Nature, Vol. 321, Issue 6069, p. 522-525
Evaluation of the safety, reactogenicity and immunogenicity of three recombinant outer surface protein (OspA) lyme vaccines in healthy adults
journal, December 1996
- Van Hoecke, C.; Comberbach, M.; De Grave, D.
- Vaccine, Vol. 14, Issue 17-18, p. 1620-1626
Expression of tetanus toxin fragment C in E.coli : high level expression by removing rare codons
journal, January 1989
- Makoff, A. J.; Oxer, M. D.; Romanos, M. A.
- Nucleic Acids Research, Vol. 17, Issue 24, p. 10191-10202
Structural identification of a key protective B-cell epitope in lyme disease antigen OspA
journal, October 2000
- Ding, Wei; Huang, Xiaolin; Yang, Xiaohua
- Journal of Molecular Biology, Vol. 302, Issue 5, p. 1153-1164
Abilities of OspA proteins from different seroprotective groups of Borrelia burgdorferi to protect hamsters from infection.
journal, January 1995
- Lovrich, S. D.; Callister, S. M.; DuChateau, B. K.
- Infection and immunity, Vol. 63, Issue 6
SV40 DNA transfection of cells in suspension: analysis of the efficiency of transcription and translation of T-antigen
journal, March 1981
- Chu, Gilbert; Sharp, Phillip A.
- Gene, Vol. 13, Issue 2, p. 197-202
Inhibition of Borrelia burgdorferi-Tick Interactions In Vivo by Outer Surface Protein A Antibody
journal, June 2001
- Pal, U.; Montgomery, R. R.; Lusitani, D.
- The Journal of Immunology, Vol. 166, Issue 12, p. 7398-7403
A comprehensive set of sequence analysis programs for the VAX
journal, January 1984
- Devereux, John; Haeberli, Paul; Smithies, Oliver
- Nucleic Acids Research, Vol. 12, Issue 1, p. 387-395
Sequence analysis of ospA genes shows homogeneity within Borrelia burgdorferi sensu stricto and Borrelia afzelii strains but reveals major subgroups within the Borrelia garinii species
journal, August 1995
- Will, G.; Jauris-Heipke, S.; Schwab, E.
- Medical Microbiology and Immunology, Vol. 184, Issue 2, p. 73-80
Immunization with a polyvalent OspA vaccine protects mice against Ixodes ricinus tick bites infected by Borrelia burgdorferi ss, Borrelia garinii and Borrelia afzelii
journal, October 1997
- Gern, Lise; Hu, Chang Min; Voet, Pierre
- Vaccine, Vol. 15, Issue 14, p. 1551-1557
Amino acid substitution matrices from protein blocks.
journal, November 1992
- Henikoff, S.; Henikoff, J. G.
- Proceedings of the National Academy of Sciences, Vol. 89, Issue 22, p. 10915-10919
Applications and statistics for multiple high-scoring segments in molecular sequences.
journal, June 1993
- Karlin, S.; Altschul, S. F.
- Proceedings of the National Academy of Sciences, Vol. 90, Issue 12, p. 5873-5877