Chimeric OspA genes, proteins and methods of use thereof

Title: Chimeric OspA genes, proteins and methods of use thereof

The invention relates to the development of chimeric OspA molecules for use in a new Lyme vaccine. More specifically, the chimeric OspA molecules comprise the proximal portion from one OspA serotype, together with the distal portion from another OspA serotype, while retaining antigenic properties of both of the parent polypeptides. The chimeric OspA molecules are delivered alone or in combination to provide protection against a variety of Borrelia genospecies. The invention also provides methods for administering the chimeric OspA molecules to a subject in the prevention and treatment of Lyme disease or borreliosis.

Inventors:: Crowe, Brian A.; Livey, Ian; O'Rourke, Maria; Schwendinger, Michael; Dunn, John J.; Luft, Benjamin J.

Issue Date:: 2018-02-20

OSTI Identifier:: 1422826

Assignee:: Brookhaven Science Associates, LLC (Upton, NY) BNL

Patent Number(s):: 9,895,434

Application Number:: 15/049,438

Contract Number:: DE-SC0012704

Resource Relation:: Patent File Date: 2016 Feb 22

Research Org:: Research Foundation of the State University of New York, Amherst, NY; Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Org:: USDOE

Country of Publication:: United States

Language:: English

Full Text
View Full Text

Have feedback or suggestions for a way to improve these results? !

Save / Share this Record no
Export Metadata
- Endnote
- RIS
- Excel
- CSV
- XML
Send to Email

Send to Email

Email address:

Content:

Works referenced in this record:

Recombinant immunoglobin preparations
patent, March 1989

Cabilly, Shmuel; Heyneker, Herbert; Holmes, William
US Patent Document 4,816,567
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F4816567

Basic local alignment search tool
journal, October 1990

Altschul, Stephen F.; Gish, Warren; Miller, Webb
Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
DOI: 10.1016/S0022-2836(05)80360-2

Characterization of diphtheria fusion proteins targeted to the human interleukin-3 receptor
journal, August 2000

Frankel, Arthur E.; Ramage, Jason; Kiser, Melanie
Protein Engineering, Design and Selection, Vol. 13, Issue 8, p. 575-581
DOI: 10.1093/protein/13.8.575

A new technique for the assay of infectivity of human adenovirus 5 DNA
journal, April 1973

Graham, F. L.; van der Eb, A. J.
Virology, Vol. 52, Issue 2, p. 456-467
DOI: 10.1016/0042-6822(73)90341-3

Amino acid substitution matrices from protein blocks.
journal, November 1992

Henikoff, S.; Henikoff, J. G.
Proceedings of the National Academy of Sciences, Vol. 89, Issue 22, p. 10915-10919
DOI: 10.1073/pnas.89.22.10915

Applications and statistics for multiple high-scoring segments in molecular sequences.
journal, June 1993

Karlin, S.; Altschul, S. F.
Proceedings of the National Academy of Sciences, Vol. 90, Issue 12, p. 5873-5877
DOI: 10.1073/pnas.90.12.5873

Continuous cultures of fused cells secreting antibody of predefined specificity
journal, August 1975

Köhler, G.; Milstein, C.
Nature, Vol. 256, Issue 5517, p. 495-497
DOI: 10.1038/256495a0

By-passing immunization: Human antibodies from V-gene libraries displayed on phage
journal, December 1991

Marks, James D.; Hoogenboom, Hennie R.; Bonnert, Timothy P.
Journal of Molecular Biology, Vol. 222, Issue 3, p. 581-597
DOI: 10.1016/0022-2836(91)90498-U

Improved tools for biological sequence comparison.
journal, April 1988

Pearson, W. R.; Lipman, D. J.
Proceedings of the National Academy of Sciences, Vol. 85, Issue 8, p. 2444-2448
DOI: 10.1073/pnas.85.8.2444

Comparison of biosequences
journal, December 1981

Smith, Temple F.; Waterman, Michael S.
Advances in Applied Mathematics, Vol. 2, Issue 4, p. 482-489
DOI: 10.1016/0196-8858(81)90046-4

From genes to protein structure and function: novel applications of computational approaches in the genomic era
journal, January 2000

Skolnick, Jeffrey; Fetrow, Jacquelyn S.
Trends in Biotechnology, Vol. 18, Issue 1, p. 34-39
DOI: 10.1016/S0167-7799(99)01398-0

Similar Records

Similar records in DOepatents and OSTI.GOV collections:

Geobacter strains that use alternate organic compounds, methods of making, and methods of use thereof

Patent Lovley, Derek R ; Summers, Zarath Morgan ; Haveman, Shelley Annette ; Izallalen, Mounir

In preferred embodiments, the present invention provides new isolated strains of Geobacter species that are capable of using a carbon source that is selected from C.sub.3 to C.sub.12 organic compounds selected from pyruvate or metabolic precursors of pyruvate as an electron donor in metabolism and in subsequent energy production. In other aspects, other preferred embodiments of the present invention include methods of making such strains and methods of using such strains. In general, the wild type strain of the microorganisms has been shown to be unable to use these C.sub.3 to C.sub.12 organic compounds as electron donors in metabolic stepsmore »« less
Full Text Available December 2013
Geobacter strains that use alternate organic compounds, methods of making, and methods of use thereof

Patent Lovley, Derek R. ; Summers, Zarath Morgan ; Haveman, Shelley Annette ; Izallalen, Mounir

In preferred embodiments, the present invention provides new isolated strains of a Geobacter species that are capable of using a carbon source that is selected from C.sub.3 to C.sub.12 organic compounds selected from pyruvate or metabolic precursors of pyruvate as an electron donor in metabolism and in subsequent energy production. The wild type strain of the microorganisms has been shown to be unable to use these C.sub.3 to C.sub.12 organic compounds as electron donors. The inventive strains of microorganisms are useful for improving bioremediation applications, including in situ bioremediation (including uranium bioremediation and halogenated solvent bioremediation), microbial fuel cells, powermore »« less
Full Text Available March 2016
Transcriptional control in Alicyclobacillus acidocaldarius and associated genes, proteins, and methods

Patent Lee, Brady Deneys ; Thompson, David N ; Apel, William A. ; Thompson, Vicki Slavchev ; Reed, David W ; Lacey, Jeffrey A

Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of modulating transcription or transcription or transcriptional control using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.
Full Text Available May 2014
Transcriptional control in Alicyclobacillus acidocaldarius and associated genes, proteins, and methods

Patent Lee, Brady D. ; Thompson, David N. ; Apel, William A. ; Thompson, Vicki S. ; Reed, David W. ; Lacey, Jeffrey A.

Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of modulating transcription or transcription or transcriptional control using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.
Full Text Available November 2015
Transcriptional control in alicyclobacillus acidocaldarius and associated genes, proteins, and methods

Patent Lee, Brady D ; Thompson, David N ; Apel, William A ; Thompson, Vicki S ; Reed, David W ; Lacey, Jeffrey A

Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of modulating transcription or transcription or transcriptional control using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.
Full Text Available November 2016