Transgenic plants with enhanced growth characteristics
Abstract
The invention relates to transgenic plants exhibiting dramatically enhanced growth rates, greater seed and fruit/pod yields, earlier and more productive flowering, more efficient nitrogen utilization, increased tolerance to high salt conditions, and increased biomass yields. In one embodiment, transgenic plants engineered to over-express both glutamine phenylpyruvate transaminase (GPT) and glutamine synthetase (GS) are provided. The GPT+GS double-transgenic plants of the invention consistently exhibit enhanced growth characteristics, with T0 generation lines showing an increase in biomass over wild type counterparts of between 50% and 300%. Generations that result from sexual crosses and/or selfing typically perform even better, with some of the double-transgenic plants achieving an astounding four-fold biomass increase over wild type plants.
- Inventors:
- Issue Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1416534
- Patent Number(s):
- 9862964
- Application Number:
- 15/256,721
- Assignee:
- Los Alamos National Security, LLC (Los Alamos, NM); University of Maine System Board of Trustees (Bangor, ME)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
- DOE Contract Number:
- AC52-06NA25396
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2016 Sep 05
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Unkefer, Pat J., Anderson, Penelope S., and Knight, Thomas J. Transgenic plants with enhanced growth characteristics. United States: N. p., 2018.
Web.
Unkefer, Pat J., Anderson, Penelope S., & Knight, Thomas J. Transgenic plants with enhanced growth characteristics. United States.
Unkefer, Pat J., Anderson, Penelope S., and Knight, Thomas J. Tue .
"Transgenic plants with enhanced growth characteristics". United States. https://www.osti.gov/servlets/purl/1416534.
@article{osti_1416534,
title = {Transgenic plants with enhanced growth characteristics},
author = {Unkefer, Pat J. and Anderson, Penelope S. and Knight, Thomas J.},
abstractNote = {The invention relates to transgenic plants exhibiting dramatically enhanced growth rates, greater seed and fruit/pod yields, earlier and more productive flowering, more efficient nitrogen utilization, increased tolerance to high salt conditions, and increased biomass yields. In one embodiment, transgenic plants engineered to over-express both glutamine phenylpyruvate transaminase (GPT) and glutamine synthetase (GS) are provided. The GPT+GS double-transgenic plants of the invention consistently exhibit enhanced growth characteristics, with T0 generation lines showing an increase in biomass over wild type counterparts of between 50% and 300%. Generations that result from sexual crosses and/or selfing typically perform even better, with some of the double-transgenic plants achieving an astounding four-fold biomass increase over wild type plants.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 09 00:00:00 EST 2018},
month = {Tue Jan 09 00:00:00 EST 2018}
}
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Works referenced in this record:
Method of Conferring Multiple Stress Tolerance and Early Flowering Plants
patent-application, January 2008
- Kav, Nataraj; Srivastava, Sanjeeva; Shah, Salehuzzaman
- US Patent Document 11/750797; 20080005810
Biosynthesis and secretion of mugineic acid family phytosiderophores in zinc-deficient barley
journal, October 2006
- Suzuki, Motofumi; Takahashi, Michiko; Tsukamoto, Takashi
- The Plant Journal, Vol. 48, Issue 1, p. 85-97
Structural Insight into the Mechanism of Substrate Specificity of Aedes Kynurenine Aminotransferase
journal, February 2008
- Han, Qian; Gao, Yi Gui; Robinson, Howard
- Biochemistry, Vol. 47, Issue 6, p. 1622-1630
Omega-amidase pathway in the degradation of glutamine in Neurospora crassa.
journal, January 1985
- Calderón, J.; Morett, E.; Mora, J.
- Journal of Bacteriology, Vol. 161, Issue 2
2 x 2 x 2 Week Dosing Regimen for Treating Actinic Keratosis with Pharmaceutical Compositions Formulated with 3.75% Imiquimod
patent-application, August 2013
- Nordsiek, Michael; Levy, Sharon; Lee, James
- US Patent Document 13/568003; 20130210855
Dual promoter of Agrobacterium tumefaciens mannopine synthase genes is regulated by plant growth hormones
journal, May 1989
- Langridge, W. H. R.; Fitzgerald, K. J.; Koncz, C.
- Proceedings of the National Academy of Sciences, Vol. 86, Issue 9, p. 3219-3223
Genetic Analysis of Protein Stability and Function
journal, December 1989
- Pakula, A. A.; Sauer, R. T.
- Annual Review of Genetics, Vol. 23, Issue 1, p. 289-310
2×2×2 week treatment regimen for treating actinic keratosis with pharmaceutical compositions formulated with 2.5% imiquimod
patent, July 2012
- Nordsiek, Michael T.; Levy, Sharon F.; Lee, James H.
- US Patent Document 8,222,270
Maize α-tubulin genes are expressed according to specific patterns of cell differentiation
journal, January 1998
- Uribe, Xavier; Torres, Miguel Angel; Capellades, Montserrat
- Plant Molecular Biology, Vol. 37, Issue 6, p. 1069-1078
[21] Electroporation of DNA and RNA into plant protoplasts
book, January 1987
- Fromm, Michael; Callis, Judy; Taylor, Loverine P.
- Methods in Enzymology, p. 351-366
Targeting transgene expression in research, agricultural, and environmental applications: Promoters used in plant transformation
journal, January 2004
- Potenza, Carol; Aleman, Lorenzo; Sengupta-Gopalan, Champa
- In Vitro Cellular & Developmental Biology - Plant, Vol. 40, Issue 1, p. 1-22
Use of prolines for improving growth and other properties of plants and algae
patent, December 2004
- Unkefer, Pat J.; Knight, Thomas J.; Martinez, Rodolfo A.
- US Patent Document 6,831,040
Rice nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement
patent-application, June 2004
- La Rosa, Thomas; Kovalic, David; Zhou, Yihua
- US Patent Document 10/437963; 20040123343
Use of prolines for improving growth and other properties of plants and algae
patent, April 2003
- Unkefer, Pat J.; Knight, Thomas J.; Martinez, Rodolfo A.
- US Patent Document 6,555,500
Method of treating actinic keratosis with 3.75% imiquimod cream
patent, October 2012
- Nordsiek, Michael T.; Levy, Sharon F.; Lee, James H.
- US Patent Document 8,299,109
Efficient transformation of Arabidopsis thaliana: comparison of the efficiencies with various organs, plant ecotypes and Agrobacterium strains
journal, December 1992
- Akama, Kazuhito; Shiraishi, Hideaki; Ohta, Shozo
- Plant Cell Reports, Vol. 12, Issue 1, p. 7-11
Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement
patent-application, January 2007
- Liu, Jingdong; Zhou, Yihua; Kovalic, David
- US Patent Document 11/520715; 20070011783
The Molecular-Genetics of Nitrogen Assimilation Into Amino Acids In Higher Plants
journal, June 1996
- Lam, H.-M.; Coschigano, K. T.; Oliveira, I. C.
- Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 47, Issue 1, p. 569-593
Glutamine synthetase genes of pea encode distinct polypeptides which are differentially expressed in leaves, roots and nodules.
journal, January 1987
- Tingey, S. V.; Walker, E. L.; Coruzzi, G. M.
- The EMBO Journal, Vol. 6, Issue 1, p. 1-9
Preparation of 2-hydroxy-5-oxoproline and analogs thereof
patent, September 2001
- Martinez, Rodolfo A.; Unkefer, Pat J.
- US Patent Document 6,288,240
Primary structure and differential expression of glutamine synthetase genes in nodules, roots and leaves of Phaseolus vulgaris
journal, July 1986
- Gebhardt, Christiane; Oliver, Jane E.; Forde, Brian G.
- The EMBO Journal, Vol. 5, Issue 7, p. 1429-1435
Tissue- and cell-specific activity of a phenylalanine ammonia-lyase promoter in transgenic plants.
journal, July 1989
- Bevan, M.; Shufflebottom, D.; Edwards, K.
- The EMBO Journal, Vol. 8, Issue 7
Enzyme Redundancy and the Importance of 2-Oxoglutarate in Higher Plant Ammonium Assimilation
journal, July 2000
- Lancien, Muriel; Gadal, Pierre; Hodges, Michael
- Plant Physiology, Vol. 123, Issue 3, p. 817-824
Genetic transformation of plants by protoplast electroporation
journal, October 1994
- Bates, George W.
- Molecular Biotechnology, Vol. 2, Issue 2
Modeling and molecular dynamics of glutamine transaminase K/cysteine conjugate β-lyase
journal, September 2003
- Venhorst, Jennifer; ter Laak, Antonius M.; Meijer, Moira
- Journal of Molecular Graphics and Modelling, Vol. 22, Issue 1, p. 55-70
Characterization of a Nodule-Enhanced Glutamine Synthetase from Alfalfa: Nucleotide Sequence, In Situ Localization, and Transcript Analysis
journal, January 1995
- Temple, Stephen J.
- Molecular Plant-Microbe Interactions, Vol. 8, Issue 2
Molecular analysis of the alcohol dehydrogenase (Adhl) gene of maize
journal, May 1984
- Dennis, E.S.; Gerlach, W.L.; Pryor, A.J.
- Nucleic Acids Research, Vol. 12, Issue 9, p. 3983-4000
Oats Tolerant of Pseudomonas syringae pv. tabaci Contain Tabtoxinine-β-Lactam-Insensitive Leaf Glutamine Synthetases
journal, October 1988
- Knight, T. J.; Bush, D. R.; Langston-Unkefer, P. J.
- Plant Physiology, Vol. 88, Issue 2, p. 333-339
Transgenic plants that exhibit enhanced nitrogen assimilation
patent, August 2000
- Coruzzi, Gloria; Brears, Timothy
- US Patent Document 6,107,547
Synthetic promoters genetic control through cis engineering
journal, March 2007
- Venter, Mauritz
- Trends in Plant Science, Vol. 12, Issue 3, p. 118-124
Plant glutamine synthetase complements a glnA mutation in Escherichia coli
journal, June 1986
- DasSarma, S.; Tischer, E.; Goodman, H. M.
- Science, Vol. 232, Issue 4755, p. 1242-1244
An Agrobacterium -transformed cell culture from the monocot Asparagus officinalis
journal, December 1984
- Hernalsteens, J. -P.; Thia-Toong, L.; Schell, J.
- The EMBO Journal, Vol. 3, Issue 13
Lower Dosage Strength Pharmaceutical Compositions Formulated with 2.5% Imiquimod
patent-application, October 2011
- Nordsiek, Michael; Levy, Sharon; Lee, James
- US Patent Document 13/179402; 20110263634
A Rapid and Simple Method of Assaying Plants Transformed with Hygromycin or PPT Resistance Genes
journal, January 1997
- Wang, Ming-Bo; Waterhouse, Peter M.
- Plant Molecular Biology Reporter, Vol. 15, Issue 3, p. 209-215
Expression of soybean lectin gene deletions in tobacco
journal, January 1990
- Lindstrom, Jon T.; Vodkin, Lila O.; Harding, Roy W.
- Developmental Genetics, Vol. 11, Issue 2, p. 160-167
Glutamine synthetase isoenzymes of Phaseolus vulgaris L. subunit composition in developing root nodules and plumules
journal, November 1989
- Bennett, Malcolm J.; Cullimore, Julie V.
- Planta, Vol. 179, Issue 4, p. 433-440
3 x 3 x 3 Week Dosing Regimen for Treating Actinic Keratosis with Pharmaceutical Compositions Formulated with 3.75% Imiquimod
patent-application, October 2011
- Nordsiek, Michael; Levy, Sharon; Lee, James
- US Patent Document 13/184556; 20110263636
Genes and uses for plant improvement
patent-application, November 2008
- Abad, Mark; Goldman, Barry; Chittoor-Vijayanath, Jaishree
Nucleic acids encoding plant glutamine phenylpyruvate transaminase (GPT) and uses thereof
patent, March 2016
- Unkefer, Pat J.; Anderson, Penelope S.; Knight, Thomas J.
- US Patent Document 9,296,998
Amino acid substitution matrices from protein blocks.
journal, November 1992
- Henikoff, S.; Henikoff, J. G.
- Proceedings of the National Academy of Sciences, Vol. 89, Issue 22, p. 10915-10919
Light-Regulated and Cell-Specific Expression of Tomato rbcS-gusA and Rice rbcS-gusA Fusion Genes in Transgenic Rice
journal, July 1993
- Kyozuka, J.; McElroy, D.; Hayakawa, T.
- Plant Physiology, Vol. 102, Issue 3, p. 991-1000
Nucleic Acids Encoding Plants Glutamine Phenylpyruvate Transaminase (GPT) and Uses Thereof
patent-application, October 2016
- Unkefer, Pat; Anderson, Penelope; Knight, Thomas
- US Patent Document 15/064329; 20160289648
Transgenic plants with enhanced growth characteristics
patent, September 2016
- Unkefer, Pat J.; Anderson, Penelope S.; Knight, Thomas J.
- US Patent Document 9,434,956
Use of prolines for improving growth and other properties of plants and algae
patent, July 2003
- Unkefer, Pat J.; Knight, Thomas J.; Martinez, Rodolfo A.
- US Patent Document 6,593,275
Transgenic Algae Engineered for Higher Performance
patent-application, September 2011
- Unkefer, Pat; Anderson, Penelope; Knight, Thomas
- US Patent Document 13/037149; 20110217780
Overproduction of alfalfa glutamine synthetase in transgenic tobacco plants
journal, June 1989
- Eckes, Peter; Schmitt, Petra; Daub, Winfried
- MGG Molecular & General Genetics, Vol. 217, Issue 2-3, p. 263-268
Crystal Structure of Human Kynurenine Aminotransferase I
journal, September 2004
- Rossi, Franca; Han, Qian; Li, Junsuo
- Journal of Biological Chemistry, Vol. 279, Issue 48, p. 50214-50220
Characterization of diphtheria fusion proteins targeted to the human interleukin-3 receptor
journal, August 2000
- Frankel, Arthur E.; Ramage, Jason; Kiser, Melanie
- Protein Engineering, Design and Selection, Vol. 13, Issue 8, p. 575-581
A gene network controlling glutamine and asparagine biosynthesis in plants.
journal, November 1991
- McGrath, R. B.; Coruzzi, G. M.
- The Plant Journal, Vol. 1, Issue 3, p. 275-280
Improved tools for biological sequence comparison.
journal, April 1988
- Pearson, W. R.; Lipman, D. J.
- Proceedings of the National Academy of Sciences, Vol. 85, Issue 8, p. 2444-2448
Overexpression of Cytosolic Glutamine Synthetase. Relation to Nitrogen, Light, and Photorespiration
journal, June 2002
- Oliveira, Igor C.; Brears, Timothy; Knight, Thomas J.
- Plant Physiology, Vol. 129, Issue 3, p. 1170-1180
A Highly Efficient Transformation Protocol for Micro-Tom, a Model Cultivar for Tomato Functional Genomics
journal, March 2006
- Sun, Hyeon-Jin; Uchii, Sayaka; Watanabe, Shin
- Plant and Cell Physiology, Vol. 47, Issue 3, p. 426-431
Lower Dosage Strength Imiquimod Formulations and Shorter Dosing Regimens for Treating Actinic Keratoses
patent-application, January 2011
- Nordsiek, Michael; Levy, Sharon; Lee, James
- US Patent Document 12/636613; 20110021555
Nucleic acids encoding plant glutamine phenylpyruvate transaminase (GPT) and uses thereof
patent-application, February 2011
- Unkefer, Pat; Anderson, Penelope; Knight, Thomas
- US Patent Document 12/660506; 20110030104
Structural and transcriptional analysis of DNA sequences flanking genes that encode 19 kilodalton zeins
journal, April 1987
- Kriz, Alan L.; Boston, Rebecca S.; Larkins, Brian A.
- MGG Molecular & General Genetics, Vol. 207, Issue 1
Fertile transgenic rice plants regenerated from transformed protoplasts
journal, March 1989
- Shimamoto, Ko; Terada, Rie; Izawa, Takeshi
- Nature, Vol. 338, Issue 6212, p. 274-276
Expression of a conifer glutamine synthetase gene in transgenic poplar
journal, November 1999
- Gallardo, Fernando; Fu, Jianming; Cantón, Francisco R.
- Planta, Vol. 210, Issue 1, p. 19-26
Analysis of a chimeric class-I patatin-GUS gene in transgenic potato plants: High-level expression in tubers and sucrose-inducible expression in cultured leaf and stem explants
journal, January 1989
- Wenzler, Herman C.; Mignery, Gregory A.; Fisher, Linda M.
- Plant Molecular Biology, Vol. 12, Issue 1, p. 41-50
Specificity of Agrobacterium-mediated delivery of maize streak virus DNA to members of the Gramineae
journal, January 1989
- Boulton, Margaret I.; Buchholz, Wallace G.; Marks, Melanie S.
- Plant Molecular Biology, Vol. 12, Issue 1, p. 31-40
Modulation of glutamine synthetase gene expression in tobacco by the introduction of an alfalfa glutamine synthetase gene in sense and antisense orientation: molecular and biochemical analysis
journal, January 1993
- Temple, Stephen J.; Knight, Thomas J.; Unkefer, Pat J.
- MGG Molecular & General Genetics, Vol. 236-236, Issue 2-3, p. 315-325
Direct gene transfer to plants
journal, December 1984
- Paszkowski, Jerzy; Shillito, Raymond D.; Saul, Michael
- The EMBO Journal, Vol. 3, Issue 12, p. 2717-2722
Transgenic Plants with Enhanced Growth Characteristics
patent-application, July 2010
- Unkefer, Pat; Anderson, Penelope; Knight, Thomas
- US Patent Document 12/551271; 20100186121
Carbon and Amino Acids Reciprocally Modulate the Expression of Glutamine Synthetase in Arabidopsis
journal, September 1999
- Oliveira, Igor C.; Coruzzi, Gloria M.
- Plant Physiology, Vol. 121, Issue 1, p. 301-310
A simple method for the production of highly competent cells ofAgrobacterium for transformation via electroporation
journal, April 1998
- McCormac, A. C.; Elliott, M. C.; Chen, D. F.
- Molecular Biotechnology, Vol. 9, Issue 2
Overexpression of a soybean gene encoding cytosolic glutamine synthetase in shoots of transgenic Lotus corniculatus L. plants triggers changes in ammonium assimilation and plant development
journal, April 1997
- Vincent, Remi; Fraisier, Vincent; Chaillou, Sylvain
- Planta, Vol. 201, Issue 4, p. 424-433
Plant Glutamine Phenylpyruvate Transaminase Gene and Transgenic Plants Carrying Same
patent-application, May 2017
- Unkefer, Pat; Anderson, Penelope; Knight, Thomas
- US Patent Document 15/233879; 20170121728
Does root glutamine synthetase control plant biomass production in Lotus japonicus L.?
journal, October 1999
- Limami, Anis; Phillipson, Belinda; Ameziane, Rafiqa
- Planta, Vol. 209, Issue 4, p. 495-502
Expression of genes transferred into monocot and dicot plant cells by electroporation.
journal, September 1985
- Fromm, M.; Taylor, L. P.; Walbot, V.
- Proceedings of the National Academy of Sciences, Vol. 82, Issue 17, p. 5824-5828
Sequence of a 21 kd zein gene from maize containing an in-frame stop codon
journal, January 1989
- Wandelt, Christine; Feix, Guenter
- Nucleic Acids Research, Vol. 17, Issue 6
Light Regulation of Gene Expression in Higher Plants
journal, June 1985
- Tobin, E. M.; Silverthorne, J.
- Annual Review of Plant Physiology, Vol. 36, Issue 1, p. 569-593
Cysteine-S-conjugate β-Lyase Activity and Pyridoxal Phosphate Binding Site of Onion Alliin Lyase
journal, January 1997
- Kitamura, Nobuko; Shimomura, Naoya; Iseki, Junji
- Bioscience, Biotechnology, and Biochemistry, Vol. 61, Issue 8, p. 1327-1330
Vascular expression of a bean cell wall glycine-rich protein-β-glucuronidase gene fusion in transgenic tobacco
journal, May 1989
- Keller, Beat; Schmid, Jürg; Lamb, Christopher J.
- The EMBO Journal, Vol. 8, Issue 5, p. 1309-1314
Transgenic plants that exhibit enhanced nitrogen assimilation
patent-application, June 2002
- Kisaka, Hiroaki; Kida, Takao
- US Patent Document 09/729821; 20020069430
GATEWAY™ vectors for Agrobacterium-mediated plant transformation
journal, May 2002
- Karimi, Mansour; Inzé, Dirk; Depicker, Ann
- Trends in Plant Science, Vol. 7, Issue 5, p. 193-195
Method of Treating Actinic Keratosis with 3.75% Imiquimod Cream
patent-application, October 2011
- Nordsiek, Michael; Levy, Sharon; Lee, James
- US Patent Document 13/182433; 20110263635
A zein gene of maize is transcribed from two widely separated promoter regions
journal, October 1983
- Langridge, Peter; Feix, Günter
- Cell, Vol. 34, Issue 3, p. 1015-1022
2 x 2 x 2 Week Treatment Regimen for Treating Actinic Keratosis With Pharmaceutical Compositions Formulated With 2.5% Imiquimod
patent-application, October 2011
- Nordsiek, Michael; Levy, Sharon; Lee, James
- US Patent Document 13/179315; 20110257216
Antibiotic resistance conferred by a plant ABC transporter gene when expressed in transgenic plants
patent, July 2011
- Stewart, C. Neal; Ayalew, Mentewab
- US Patent Document 7,973,213
Nucleic acids encoding plant glutamine phenylpyruvate transaminase (GPT) and uses thereof
patent-application, July 2010
- Unkefer, Pat; Anderson, Penelope; Knight, Thomas
- US Patent Document 12/551193; 20100170009
25324, 50287, 28899, 47007, and 42967 transferase family members and uses therefor
patent-application, February 2002
- Meyers, Rachel; MacBeth, Kyle; Rudolph-Owen, Laura
- US Patent Document 09/798029; 20020019030
Transgenic Algae Engineered for Higher Performance
patent-application, May 2016
- Unkefer, Pat; Anderson, Penelope; Knight, Thomas
- US Patent Document 14/515773; 20160137972
Applications and statistics for multiple high-scoring segments in molecular sequences.
journal, June 1993
- Karlin, S.; Altschul, S. F.
- Proceedings of the National Academy of Sciences, Vol. 90, Issue 12, p. 5873-5877
The glutamine synthetase gene family of Arabidopsis thaliana light-regulation and differential expression in leaves, roots and seeds
journal, November 1991
- Peterman, T. Kaye; Goodman, Howard M.
- MGG Molecular & General Genetics, Vol. 230, Issue 1-2
Electrotransformation of Chlorella vulgaris
journal, May 1999
- Chow, K.-C.; Tung, W. L.
- Plant Cell Reports, Vol. 18, Issue 9, p. 778-780
Characterization of an rbcS gene from Nicotiana plumbaginifolia and expression of an rbcS-CAT chimeric gene in homologous and heterologous nuclear background
journal, November 1986
- Poulsen, Carsten; Fluhr, Robert; Kauffman, John M.
- MGG Molecular & General Genetics, Vol. 205, Issue 2, p. 193-200
Structure and expression of the maize gene encoding the phosphoenolpyruvate carboxylase isozyme involved in C4 photosynthesis
journal, May 1989
- Hudspeth, Richard L.; Grula, John W.
- Plant Molecular Biology, Vol. 12, Issue 5, p. 579-589
The Glutamine Transaminase-ω-Amidase Pathwa
journal, January 1977
- Cooper, Arthur J. L.; Meister, Alton; Snell, Esmond E.
- CRC Critical Reviews in Biochemistry, Vol. 4, Issue 3
Molecular approaches to the study of amino acid biosynthesis in plants
journal, January 1991
- Coruzzi, G. M.
- Plant Science, Vol. 74, Issue 2, p. 145-155
Molecular and general genetics of a hybrid foreign gene introduced into tobacco by direct gene transfer
journal, January 1985
- Potrykus, Ingo; Paszkowski, Jerzy; Saul, Michael W.
- MGG Molecular & General Genetics, Vol. 199, Issue 2, p. 169-177
Cell-specific expression in transgenic plants reveals nonoverlapping roles for chloroplast and cytosolic glutamine synthetase.
journal, May 1990
- Edwards, J. W.; Walker, E. L.; Coruzzi, G. M.
- Proceedings of the National Academy of Sciences, Vol. 87, Issue 9
Preferential Expression of an α-Tubulin Gene of Arabidopsis in Pollen
journal, May 1992
- Carpenter, J. L.
- THE PLANT CELL ONLINE, Vol. 4, Issue 5, p. 557-571
Photosynthesis-Associated Gene Families: Differences in Response to Tissue-Specific and Environmental Factors
journal, July 1986
- Simpson, June; van Montagu, Marc; Herrera-Estrella, Luis
- Science, Vol. 233, Issue 4759, p. 34-38
Plant glutamine phenylpyruvate transaminase gene and transgenic plants carrying same
patent-application, January 2011
- Unkefer, Pat; Anderson, Penelope; Knight, Thomas
- US Patent Document 12/660508; 20110004961
Technical Focus: A guide to Agrobacterium binary Ti vectors
journal, October 2000
- Hellens, Roger; Mullineaux, Philip; Klee, Harry
- Trends in Plant Science, Vol. 5, Issue 10, p. 446-451
Developmentally Regulated Expression of the Gene Family for Cytosolic Glutamine Synthetase in Pisum sativum
journal, October 1989
- Walker, E. L.; Coruzzi, G. M.
- Plant Physiology, Vol. 91, Issue 2, p. 702-708
A promoter sequence involved in cell-specific expression of the pea glutamine synthetase GS3A gene in organs of transgenic tobacco and alfalfa
journal, September 1991
- Brears, Timothy; Walker, Elsbeth L.; Coruzzi, Gloria M.
- The Plant Journal, Vol. 1, Issue 2, p. 235-244
Enhanced evolutionary PCR using oligonucleotides with inosine at the 3′-terminus
journal, January 1991
- Batzer, Mark A.; Carlton, James E.; Deininger, Prescott L.
- Nucleic Acids Research, Vol. 19, Issue 18, p. 5081-5081
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
journal, January 1988
- Saiki, R. K.; Gelfand, D. H; Stoffel, S.
- Science, Vol. 239, Issue 4839, p. 487-491
Shorter T-DNA or additional virulence genes improve Agrobactrium-mediated transformation
journal, November 2000
- Park, S. H.; Lee, B. -M.; Salas, M. G.
- Theoretical and Applied Genetics, Vol. 101, Issue 7
Use of prolines for improving growth and/or yield
patent-application, May 2007
- Unkefer, Pat; Martinez, Rodolfo; Knight, Thomas
- US Patent Document 11/269417; 20070105719
Molecular cloning and expression of a cDNA for human kidney cysteine conjugate β-lyase
journal, March 1995
- Perry, Sandra; Harries, Helen; Scholfield, Claire
- FEBS Letters, Vol. 360, Issue 3, p. 277-280
Molecular analysis of three maize 22 kDa auxin-binding protein genes - transient promoter expression and regulatory regions
journal, September 1993
- Schwob, Etienne; Choi, Soon-Yong; Simmons, Carl
- The Plant Journal, Vol. 4, Issue 3, p. 423-432
[130] Glutamine synthetase Escherichia coli
book, January 1970
- Shapiro, Bennett M.; Stadtman, E.R.
- Methods in Enzymology, Part of volume Metabolism of Amino Acids and Amines Part A, p. 910-922
Three Methods for the Introduction of Foreign DNA into Agrobacterium
book, June 2006
- Binns, Andrew N.; Wise, Arlene A.; Liu, Zhenying
- Agrobacterium Protocols, Vol. 343, p. 43-54
Three cDNA sequences coding for glutamine synthetase polypeptides in Oryza sativa L.
journal, November 1989
- Sakamoto, Atsushi; Ogawa, Masahiro; Masumura, Takehiro
- Plant Molecular Biology, Vol. 13, Issue 5, p. 611-614
Enzymes having transaminase and aminotransferase activity and methods of use thereof
patent, September 2002
- Short, Jay; Warren, Patrick; Swanson, Ronald
- US Patent Document 09/905173; 20020132295
Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter
journal, February 1985
- Odell, Joan T.; Nagy, Ferenc; Chua, Nam-Hai
- Nature, Vol. 313, Issue 6005, p. 810-812
Sequence analysis of a genomic clone encoding a Zc2 protein from Zea mays W64 A
journal, January 1990
- Reina, M.; Ponte, I.; Guillén, P.
- Nucleic Acids Research, Vol. 18, Issue 21
Comparison of biosequences
journal, December 1981
- Smith, Temple F.; Waterman, Michael S.
- Advances in Applied Mathematics, Vol. 2, Issue 4, p. 482-489
Plant cells resistant to herbicidal glutamine synthetase inhibitors
patent, September 1992
- Goodman, Howard; Donn, Gunter
- US Patent Document 5,145,777
Metabolite and light regulation of metabolism in plants: lessons from the study of a single biochemical pathway
journal, May 2001
- Oliveira, I.C.; Brenner, E.; Chiu, J.
- Brazilian Journal of Medical and Biological Research, Vol. 34, Issue 5, p. 567-575
Transformation of Zea mays L. Using Agrobacterium tumefaciens and the Shoot Apex
journal, February 1991
- Gould, J.; Devey, M.; Hasegawa, O.
- Plant Physiology, Vol. 95, Issue 2, p. 426-434
Enzymes
patent-application, September 2005
- Yang, Junming; Dyung Lu, Aina; Yue, Henry
- US Patent Document 10/491183; 20050191627
The inhibitory effect of oak leaf litter tannins on the growth of fungi, in relation to litter decomposition
journal, August 1971
- Harrison, A. F.
- Soil Biology and Biochemistry, Vol. 3, Issue 3, p. 167-172
In Planta Transformation of Tomato
journal, June 2008
- Yasmeen, Abida; Mirza, Bushra; Inayatullah, Samia
- Plant Molecular Biology Reporter, Vol. 27, Issue 1, p. 20-28
Nitrate regulation of metabolism and growth
journal, June 1999
- Stitt, Mark
- Current Opinion in Plant Biology, Vol. 2, Issue 3, p. 178-186
GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
journal, December 1987
- Jefferson, R. A.; Kavanagh, T. A.; Bevan, M. W.
- The EMBO Journal, Vol. 6, Issue 13
Sequence-determined DNA fragments and corresponding polypeptides encoded thereby
patent-application, May 2006
- Alexandrov, Nickolai; Brover, Vyacheslav
- US Patent Document 10/953349; 20060107345
Synthesis of 2-oxoglutaramate from L-glutamine
patent-application, February 2003
- Lalonde, James
- US Patent Document 09/928810; 20030032149
Genes responsible for the supervirulence phenotype of Agrobacterium tumefaciens A281.
journal, October 1987
- Jin, S. G.; Komari, T.; Gordon, M. P.
- Journal of Bacteriology, Vol. 169, Issue 10, p. 4417-4425
Identification of several soybean cytosolic glutamine synthetase transcripts highly or specifically expressed in nodules: expression studies using one of the corresponding genes in transgenic Lotus corniculatus
journal, January 1995
- Marsolier, Marie-Claude; Debrosses, Guilhem; Hirel, Bertrand
- Plant Molecular Biology, Vol. 27, Issue 1, p. 1-15
Cloning and characterization of a nodule-enhanced glutamine synthetase-encoding gene from Lupinus luteus
journal, December 1993
- Boron, Leszek J.; Legocki, Andrzej B.
- Gene, Vol. 136, Issue 1-2, p. 95-102
Molecular cloning of rat kynurenine aminotransferase: Identity with glutamine transaminase K
journal, October 1994
- Mosca, Monica; Cozzi, Liviana; Breton, Jerome
- FEBS Letters, Vol. 353, Issue 1, p. 21-24
Isolation and characterization of a maize chlorophyll a/b binding protein gene that produces high levels of mRNA in the dark
journal, February 1989
- Sullivan, Thomas D.; Christensen, Alan H.; Quail, Peter H.
- MGG Molecular & General Genetics, Vol. 215, Issue 3, p. 431-440
Lower Dosage Strength Pharmaceutical Compositions Formulated With 3.75% Imiquimod
patent-application, October 2011
- Nordsiek, Michael; Levy, Sharon; Lee, James
- US Patent Document 13/181507; 20110257219
3 x 3 x 3 Week Treatment Regimen for Treating Actinic Keratosis With Pharmaceutical Compositions Formulated With 2.5% Imiquimod
patent-application, October 2011
- Nordsiek, Michael; Levy, Sharon; Lee, James
- US Patent Document 13/179363; 20110257217
Methods and compositions for transformation of cereals using cultured shoot meristematic tissue
patent, November 2002
- Zhang, Shibo; Cho, Myeong-Je; Bregitzer, Phillip
- US Patent Document 6,486,384
Transgenic plants with enhanced growth characteristics
patent-application, February 2011
- Unkefer, Pat; Anderson, Penelope; Knight, Thomas
- US Patent Document 12/660501; 20110030089
Nucleic Acids and Proteins and Methods for Making and Using Them
patent-application, January 2010
- Mathur, Eric; Chang, Cathy
- US Patent Document 11/817403; 20100011456
Plants having enhanced nitrogen assimilation/metabolism
patent, July 2000
- Good, Allen G.; Stroeher, Virginia L.; Muench, Douglas G.
- US Patent Document 6,084,153
Structure–function relationships of glutamine synthetases
journal, March 2000
- Eisenberg, David; Gill, Harindarpal S.; Pfluegl, Gaston M.U.
- Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 1477, Issue 1-2, p. 122-145
Plants having enhanced nitrogen assimilation/metabolism
patent-application, January 2005
- Good, Allen G.; Stroeher, Virginia L.; Muench, Douglas G.
- US Patent Application 10/321718; 20050015828
Crystal Structures of Glutamine:Phenylpyruvate Aminotransferase from Thermus thermophilus HB8 INDUCED FIT AND SUBSTRATE RECOGNITION
journal, February 2004
- Goto, Masaru; Omi, Rie; Miyahara, Ikuko
- Journal of Biological Chemistry, Vol. 279, Issue 16, p. 16518-16525
Molecules for diagnostics and therapeutics
patent-application, January 2004
- Hodgson, David; Lincoln, Stephen; Russo, Frank
- US Patent Document 10/378029; 20040014087
Gene transfer in plants: Production of transformed plants using Ti plasmid vectors
journal, January 1986
- Rogers, Stephen G.; Horsch, Robert B.; Fraley, Robert T.
- Plant Molecular Biology, Vol. 118, p. 627-640
Expression of a maize proteinase inhibitor gene is induced in response to wounding and fungal infection: systemic wound-response of a monocot gene
journal, August 1994
- Cordero, Maria Jose; Raventos, Dora; San Segundo, Blanca
- The Plant Journal, Vol. 6, Issue 2, p. 141-150
Nucleic Acids Encoding Plant Glutamine Phenylpyruvate Transaminase (GPT) and Uses Thereof
patent-application, August 2013
- Unkefer, Pat; Anderson, Penelope; Knight, Thomas
- US Patent Document 13/734688; 20130198908
Leaf-specific overexpression of plastidic glutamine synthetase stimulates the growth of transgenic tobacco seedlings
journal, January 2000
- Migge, Andrea; Carrayol, Elisa; Hirel, Bertrand
- Planta, Vol. 210, Issue 2, p. 252-260
Over‐expression of cytosolic glutamine synthetase increases photosynthesis and growth at low nitrogen concentrations
journal, May 2001
- Fuentes, Sara I.; Allen, Damian J.; Ortiz‐Lopez, Adriana
- Journal of Experimental Botany, Vol. 52, Issue 358, p. 1071-1081
[15] Improved vectors for plant transformation: Expression cassette vectors and new selectable markers
journal, January 1987
- Rogers, S. G.; Klee, H. J.; Horsch, R. B.
- Methods in Enzymology, Vol. 153, p. 253-277
Transient and Stable Expression of the Firefly Luciferase Gene in Plant Cells and Transgenic Plants
journal, November 1986
- OW, D. W.; DE WET, J. R.; HELINSKI, D. R.
- Science, Vol. 234, Issue 4778, p. 856-859
The effect of additional virulence genes on transformation efficiency, transgene integration and expression in rice plants using the pGreen/pSoup dual binary vector system
journal, December 2004
- Vain, Philippe; Harvey, Alison; Worland, Barbara
- Transgenic Research, Vol. 13, Issue 6, p. 593-603
Plant Glutamine Phenylpyruvate Transaminase Gene and Transgenic Plants Carrying Same
patent-application, October 2010
- Unkefer, Pat; Anderson, Penelope; Knight, Thomas
- US Patent Document 12/551320; 20100263090
Nitrogen-efficient monocot plants
patent-application, July 2007
- Good, Allen; Depauw, Mary; Kridl, Jean
- US Patent Application 11/644321; 20070162995
Electroporation: Mechanism and transient expression, stable transformation and biological effects in plant protoplasts
journal, February 1991
- Joersbo, Morten; Brunstedt, Janne
- Physiologia Plantarum, Vol. 81, Issue 2, p. 256-264
Plastid transformation
patent-application, December 2004
- Reddy, Siva; Sadhu, Leelawathi; Shukla, Vijaya
- US Patent Document 10/149533; 20040253586
Transgenic algae engineered for higher performance
patent, October 2014
- Unkefer, Pat J.; Anderson, Penelope S.; Knight, Thomas J.
- US Patent Document 8,865,451
The role of glutamine synthetase and glutamate dehydrogenase in nitrogen assimilation and possibilities for improvement in the nitrogen utilization of crops
journal, April 2002
- Miflin, Ben J.; Habash, Dimah Z.
- Journal of Experimental Botany, Vol. 53, Issue 370, p. 979-987
Use of deoxyinosine-containing primers vs degenerate primers for polymerase chain reaction based on ambiguous sequence information
journal, April 1994
- Rossolini, Gian Maria; Cresti, Stefania; Ingianni, Angela
- Molecular and Cellular Probes, Vol. 8, Issue 2, p. 91-98
Ca lectin gene insertion has the structural features of a transposable element
journal, October 1983
- Vodkin, Lila O.; Rhodes, Patsy R.; Goldberg, Robert B.
- Cell, Vol. 34, Issue 3, p. 1023-1031
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
journal, September 1997
- Altschul, Stephen F.; Madden, Thomas L.; Schäffer, Alejandro A.
- Nucleic Acids Research, Vol. 25, Issue 17, p. 3389-3402
Enabling technologies for manipulating multiple genes on complex pathways
journal, January 2001
- Halpin, Claire; Barakate, Abdellah; Askari, Barak M.
- Plant Molecular Biology, Vol. 47, Issue 1-2, p. 295-310
Transient expression from cab-m1 and rbcS-m3 promoter sequences is different in mesophyll and bundle sheath cells in maize leaves.
journal, April 1992
- Bansal, K. C.; Viret, J. F.; Haley, J.
- Proceedings of the National Academy of Sciences, Vol. 89, Issue 8, p. 3654-3658
2×2×2 week dosing regimen for treating actinic keratosis with pharmaceutical compositions formulated with 3.75 % imiquimod
patent, August 2012
- Nordsiek, Michael T.; Levy, Sharon F.; Lee, James H.
- US Patent Document 8,236,816
Structural model for the reaction mechanism of glutamine synthetase, based on five crystal structures of enzyme-substrate complexes
journal, January 1994
- Liaw, Shwu Huey; Eisenberg, David
- Biochemistry, Vol. 33, Issue 3, p. 675-681
Expression of Ti plasmid genes in monocotyledonous plants infected with Agrobacterium tumefaciens
journal, October 1984
- Hooykaas-Van Slogteren, G. M. S.; Hooykaas, P. J. J.; Schilperoort, R. A.
- Nature, Vol. 311, Issue 5988, p. 763-764
Structure, Evolution, and Regulation of RbcS Genes in Higher Plants
journal, June 1989
- Dean, C.; Pichersky, E.; Dunsmuir, P.
- Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 40, Issue 1
Biological functions of proline in morphogenesis and osmotolerance revealed in antisense transgenic Arabidopsis thaliana
journal, April 1999
- Nanjo, Tokihiko; Kobayashi, Masatomo; Yoshiba, Yoshu
- The Plant Journal, Vol. 18, Issue 2, p. 185-193
Genes and uses for plant improvement
patent-application, March 2009
- Goldman, Barry; Darveaux, Bettina; Cleveland, Jaclyn
- US Patent Document 11/330903; 20090070897
Nucleotide sequences and corresponding polypeptides conferring modulated plant characteristics
patent-application, April 2009
- Troukhan, Maxim; Mascia, Peter
- US Patent Document 12/286964; 20090094717
Localization of tobacco cytosolic glutamine synthetase enzymes and the corresponding transcripts shows organ- and cell-specific patterns of protein synthesis and gene expression
journal, July 1996
- Dubois, Frederic; Brugiere, Norbert; Sangwan, Rajbir S.
- Plant Molecular Biology, Vol. 31, Issue 4, p. 803-817
Two Regulatory Genes of the Maize Anthocyanin Pathway Are Homologous: Isolation of B Utilizing R Genomic Sequences
journal, December 1989
- Chandler, V. L.; Radicella, J. P.; Robbins, T.P
- THE PLANT CELL ONLINE, Vol. 1, Issue 12, p. 1175-1183
Nucleotide sequence of an alfalfa glutamine synthetase gene
journal, May 1986
- Tischer, Edmund; DasSarma, Shiladitya; Goodman, Howard M.
- MGG Molecular & General Genetics, Vol. 203, Issue 2, p. 221-229
Drug metabolizing enzymes
patent-application, June 2004
- Baugh, Mariah; Bruns, Christopher; Das, Debopriya
- US Patent Document 10/343593; 20040110259
Chlorophyllous totipotent cell cultures
patent-application, July 2005
- Jayakumar, Pon Samuel; Hopkins, Nicole Linda; Petolino, Joseph
- US Patent Document 11/031896; 20050155119
Plant Glutamine Phenylpyruvate Transaminase Gene and Transgenic Plants Carrying Same
patent-application, September 2013
- Unkefer, Pat; Anderson, Penelope; Knight, Thomas
- US Patent Document 13/718214; 20130232641
A general method applicable to the search for similarities in the amino acid sequence of two proteins
journal, March 1970
- Needleman, Saul B.; Wunsch, Christian D.
- Journal of Molecular Biology, Vol. 48, Issue 3, p. 443-453
Two classes of differentially regulated glutamine synthetase genes are expressed in the soybean nodule: a nodule-specific class and a constitutively expressed class
journal, September 1993
- Roche, Dominique; Temple, Stephen J.; Sengupta-Gopalan, Champa
- Plant Molecular Biology, Vol. 22, Issue 6, p. 971-983
Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement
patent-application, February 2007
- Kovalic, David; Andersen, Scott; Byrum, Joseph
- US Patent Document 10/703032; 20070044171
The structure and expression of maize genes encoding the major heat shock protein, hsp70
journal, March 1986
- Rochester, Dean E.; Winer, Jill A.; Shah, Dilip M.
- The EMBO Journal, Vol. 5, Issue 3
Structure, evolution and anaerobic regulation of a nuclear gene encoding cytosolic glyceraldehyde-3-phosphate dehydrogenase from maize
journal, August 1989
- Martinez, Pascal; Martin, William; Cerff, Rüdiger
- Journal of Molecular Biology, Vol. 208, Issue 4, p. 551-565
Transgenic Plants with Enhanced Growth Characteristics
patent-application, September 2013
- Unkefer, Pat J.
- US Patent Application 13/714948; 20130239256
Agrobacterium-mediated delivery of infectious maize streak virus into maize plants
journal, January 1987
- Grimsley, Nigel; Hohn, Thomas; Davies, Jeffrey W.
- Nature, Vol. 325, Issue 6100, p. 177-179
Isolation of Transcriptionally Regulated Root-Specific Genes from Tobacco
journal, July 1990
- Conkling, M. A.; Cheng, C.-l.; Yamamoto, Y. T.
- Plant Physiology, Vol. 93, Issue 3, p. 1203-1211
Cytosolic Glutamine Synthetase in Soybean Is Encoded by a Multigene Family, and the Members Are Regulated in an Organ-Specific and Developmental Manner
journal, January 2002
- Morey, K. J.; Ortega, J. L.; Sengupta-Gopalan, C.
- Plant Physiology, Vol. 128, Issue 1, p. 182-193
Acetosyringone promotes high efficiency transformation of Arabidopsis thaliana explants by Agrobacterium tumefaciens
journal, January 1987
- Sheikholeslam, Shahla N.; Weeks, Donald P.
- Plant Molecular Biology, Vol. 8, Issue 4
Cloning of the two chalcone flavanone isomerase genes from Petunia hybrida: coordinate, light-regulated and differential expression of flavonoid genes.
journal, May 1988
- van Tunen, A. J.; Koes, R. E.; Spelt, C. E.
- The EMBO Journal, Vol. 7, Issue 5
The duplicated chalcone synthase genes C2 and Whp (white pollen) of Zea mays are independently regulated; evidence for translational control of Whp expression by the anthocyanin intensifying gene in.
journal, September 1991
- Franken, P.; Niesbach-Klösgen, U.; Weydemann, U.
- The EMBO Journal, Vol. 10, Issue 9, p. 2605-2612
Forcing expression of a soybean root glutamine synthetase gene in tobacco leaves induces a native gene encoding cytosolic enzyme
journal, October 1992
- Hirel, Bertrand; Marsolier, Marie C.; Hoarau, Anne
- Plant Molecular Biology, Vol. 20, Issue 2, p. 207-218
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
journal, December 1998
- Clough, Steven J.; Bent, Andrew F.
- The Plant Journal, Vol. 16, Issue 6, p. 735-743
Up to Six Weeks Treatment Regimen for Treating Actinic Keratoses with Pharmaceutical Compositions Formulated with 2.5% Imiquimod
patent-application, October 2011
- Nordsiek, Michael; Levy, Sharon; Lee, James
- US Patent Document 13/179336; 20110263633
Genes and uses for plant improvement
patent-application, April 2006
- Cleveland, Jaclyn; Slaten, Erin; Sayed, Mahmood
- US Patent Document 11/188298; 20060075522
2 x 2 x 2 Week Dosing Regimen for Treating Actinic Keratosis With Pharmaceutical Compositions Formulated With 3.75% Imiquimod
patent-application, October 2011
- Nordsiek, Michael; Levy, Sharon; Lee, James
- US Patent Document 13/181499; 20110257218
Synthese von 1.5-Diaza-cyclooctan-dion-(4.8)-dicarbonsäure-(2.6)
journal, April 1954
- Weygand, Friedrich; Dietrich, Heinz-Jürgen
- Chemische Berichte, Vol. 87, Issue 4, p. 482-488
The maize GapC4 promoter confers anaerobic reporter gene expression and shows homology to the maize anthocyanin regulatory locus C1
journal, December 1995
- Kohler, Uwe; Liaud, Marie-Francoise; Mendel, Ralf R.
- Plant Molecular Biology, Vol. 29, Issue 6, p. 1293-1298
Up to Six Weeks Dosing Regimen for Treating Actinic Keratosis with Pharmaceutical Compositions Formulated with 3.75% Imiquimod
patent-application, October 2011
- Nordsiek, Michael; Levy, Sharon; Lee, James
- US Patent Document 13/184579; 20110263637
Strong functional GC pressure in a light-regulated maize gene encoding subunit GAPA of chloroplast glyceraldehyde-3-phosphate dehydrogenase: Implications for the evolution of GAPA pseudogenes
journal, November 1989
- Quigley, Françoise; Brinkmann, Henner; Martin, William F.
- Journal of Molecular Evolution, Vol. 29, Issue 5, p. 412-421