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Title: Engineered microbes and methods for microbial oil production

Abstract

Some aspects of this invention provide engineered microbes for oil production. Methods for microbe engineering and for use of engineered microbes are also provided herein. In some embodiments, microbes are provided that are engineered to modulate a combination of rate-controlling steps of lipid synthesis, for example, a combination of a step generating metabolites, acetyl-CoA, ATP or NADPH for lipid synthesis (a push step), and a step sequestering a product or an intermediate of a lipid synthesis pathway that mediates feedback inhibition of lipid synthesis (a pull step). Such push-and-pull engineered microbes exhibit greatly enhanced conversion yields and TAG synthesis and storage properties.

Inventors:
; ;
Issue Date:
Research Org.:
Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1416529
Patent Number(s):
9862977
Application Number:
14/547,304
Assignee:
Massachusetts Institute of Technology (Cambridge, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
DOE Contract Number:  
AR0000059
Resource Type:
Patent
Resource Relation:
Patent File Date: 2014 Nov 19
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS

Citation Formats

Stephanopoulos, Gregory, Tai, Mitchell, and Chakraborty, Sagar. Engineered microbes and methods for microbial oil production. United States: N. p., 2018. Web.
Stephanopoulos, Gregory, Tai, Mitchell, & Chakraborty, Sagar. Engineered microbes and methods for microbial oil production. United States.
Stephanopoulos, Gregory, Tai, Mitchell, and Chakraborty, Sagar. Tue . "Engineered microbes and methods for microbial oil production". United States. https://www.osti.gov/servlets/purl/1416529.
@article{osti_1416529,
title = {Engineered microbes and methods for microbial oil production},
author = {Stephanopoulos, Gregory and Tai, Mitchell and Chakraborty, Sagar},
abstractNote = {Some aspects of this invention provide engineered microbes for oil production. Methods for microbe engineering and for use of engineered microbes are also provided herein. In some embodiments, microbes are provided that are engineered to modulate a combination of rate-controlling steps of lipid synthesis, for example, a combination of a step generating metabolites, acetyl-CoA, ATP or NADPH for lipid synthesis (a push step), and a step sequestering a product or an intermediate of a lipid synthesis pathway that mediates feedback inhibition of lipid synthesis (a pull step). Such push-and-pull engineered microbes exhibit greatly enhanced conversion yields and TAG synthesis and storage properties.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {1}
}

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Works referenced in this record:

Improving the lipid accumulation properties of the yeast cells for biodiesel production using molasses
journal, October 2010


Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design
journal, August 2005


Hydrophobic substrate utilisation by the yeast Yarrowia lipolytica, and its potential applications
journal, April 2005


Promoter proximal splice sites enhance transcription
journal, November 2002


Fatty acid production in genetically modified cyanobacteria
journal, April 2011


Toward a science of metabolic engineering
journal, June 1991


Lignocellulosic Biomass Pretreatment Using AFEX
book, January 2009


Metabolic-flux and network analysis in fourteen hemiascomycetous yeasts
journal, April 2005


Enhancement of lipid production using biochemical, genetic and transcription factor engineering approaches
journal, April 2009


Lipid particle composition of the yeast Yarrowia lipolytica depends on the carbon source
journal, March 2006


Yarrowia lipolytica as a model for bio-oil production
journal, November 2009


Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine
journal, September 1999


Exon-intron structure of genes in complete fungal genomes
journal, February 2009


Influence of Glucose and Saturated Free-Fatty Acid Mixtures on Citric Acid and Lipid Production by Yarrowia lipolytica
journal, January 2006


Correlation of Lipid Accumulation in Yeasts with Possession of ATP: Citrate Lyase
journal, November 1981


Developments in Directed Evolution for Improving Enzyme Functions
journal, August 2007


A handful of intron-containing genes produces the lion's share of yeast mRNA
journal, September 1999


Lipid-induced cell dysfunction and cell death: Lessons from yeast
journal, December 2007


Advancing oleaginous microorganisms to produce lipid via metabolic engineering technology
journal, October 2013


Prediction of protein function from protein sequence and structure
journal, January 1999


Fatty acid synthesis and elongation in yeast
journal, March 2007


Elevated stearoyl-CoA desaturase-1 expression in skeletal muscle contributes to abnormal fatty acid partitioning in obese humans
journal, October 2005


Overexpression of Acetyl-CoA Carboxylase Gene of Mucor rouxii Enhanced Fatty Acid Content in Hansenula polymorpha
journal, March 2009


Enhancement of single cell oil production by Yarrowia lipolytica growing in the presence of Teucrium polium L. aqueous extract
journal, January 1999


Molecular evolution of eukaryotic genomes: hemiascomycetous yeast spliceosomal introns
journal, February 2003


How introns influence and enhance eukaryotic gene expression
journal, April 2003


Single cell oil production by Yarrowia lipolytica growing on an industrial derivative of animal fat in batch cultures
journal, March 2002


Control of Lipid Accumulation in the Yeast Yarrowia lipolytica
journal, October 2008


Challenges in Engineering Microbes for Biofuels Production
journal, February 2007


Modeling Lipid Accumulation and Degradation in Yarrowia lipolytica Cultivated on Industrial Fats
journal, June 2003


Regulation of acetyl-CoA carboxylase Table 1
journal, April 2006


Obese Yeast: Triglyceride Lipolysis Is Functionally Conserved from Mammals to Yeast
journal, November 2005


Design of an efficient medium for heterologous protein production in Yarrowia lipolytica: case of human interferon alpha 2b
journal, January 2011


Transcriptomic Analyses during the Transition from Biomass Production to Lipid Accumulation in the Oleaginous Yeast Yarrowia lipolytica
journal, November 2011


Expanding the metabolic engineering toolbox more options to engineer cells
journal, March 2007


Overproduction of Acetyl-CoA Carboxylase Activity Increases the Rate of Fatty Acid Biosynthesis in Escherichia coli
journal, July 2000


Direct conversion of inulin into single cell protein by the engineered Yarrowia lipolytica carrying inulinase gene
journal, July 2011


Model-driven evaluation of the production potential for growth-coupled products of Escherichia coli
journal, May 2010


Introns increase gene expression in cultured maize cells.
journal, December 1987


Targeting of the Arabidopsis Homomeric Acetyl-Coenzyme A Carboxylase to Plastids of Rapeseeds
journal, January 1997


Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different
journal, April 2001


Accumulation of a Cocoa-Butter-Like Lipid by Yarrowia lipolytica Cultivated on Agro-Industrial Residues
journal, February 2003


Conversion of proteins into biofuels by engineering nitrogen flux
journal, March 2011


Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids
journal, November 1998


Physiology and genetics of the dimorphic fungus Yarrowia lipolytica
journal, April 1997


Increased fatty acid production in potato by engineering of acetyl-CoA carboxylase
journal, March 2004


Yarrowia lipolytica as a potential producer of citric acid from raw glycerol
journal, April 2002


One-step transformation of the dimorphic yeast Yarrowia lipolytica
journal, August 1997


Lipid production by Yarrowia lipolytica growing on industrial glycerol in a single-stage continuous culture
journal, March 2002


A Single Mutation Converts a Novel Phosphotyrosine Binding Domain into a Dual-specificity Phosphatase
journal, November 1995


An overview of lipid metabolism in yeasts and its impact on biotechnological processes
journal, March 2011


Heterologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica: a review
journal, April 2004


Manufacturing Molecules Through Metabolic Engineering
journal, December 2010


Regulation of Fatty Acid Synthesis
journal, June 1997


Overproduction of fatty acids in engineered Saccharomyces cerevisiae
journal, April 2014


Yarrowia lipolytica, a yeast genetic system to study mitochondrial complex I
journal, September 2002


Lipid production from Yarrowia lipolytica Po1g grown in sugarcane bagasse hydrolysate
journal, October 2011


Protein expression and secretion in the yeast Yarrowia lipolytica
journal, August 2002


Co-expression of heterologous desaturase genes in Yarrowia lipolytica
journal, September 2010


Selection of Direct Transesterification as the Preferred Method for Assay of Fatty Acid Content of Microalgae
journal, September 2010


Driving Forces Enable High-Titer Anaerobic 1-Butanol Synthesis in Escherichia coli
journal, March 2011


The DGA1 Gene Determines a Second Triglyceride Synthetic Pathway in Yeast
journal, December 2001


The role of stearoyl-CoA desaturase in the control of metabolism
journal, July 2005


Comprehensive Algorithm for Quantitative Real-Time Polymerase Chain Reaction
journal, October 2005


Increasing unsaturated fatty acid contents in Escherichia coli by coexpression of three different genes
journal, February 2010


Polypeptide Release Factors in Prokaryotes and Eukaryotes
journal, January 2002


Regulation of stearoyl-CoA desaturases and role in metabolism
journal, March 2004


Dilute Acid and Autohydrolysis Pretreatment
book, August 2009


Perspectives of microbial oils for biodiesel production
journal, August 2008


The use of Yarrowia lipolytica to reduce pollution in olive mill wastewaters
journal, October 1997


Expression of inulinase gene in the oleaginous yeast Yarrowia lipolytica and single cell oil production from inulin-containingmaterials
journal, November 2010