Title: Engineered microbes and methods for microbial oil production

Abstract

Some aspects of this invention provide engineered microbes for oil production. Methods for microbe engineering and for use of engineered microbes are also provided herein. In some embodiments, microbes are provided that are engineered to modulate a combination of rate-controlling steps of lipid synthesis, for example, a combination of a step generating metabolites, acetyl-CoA, ATP or NADPH for lipid synthesis (a push step), and a step sequestering a product or an intermediate of a lipid synthesis pathway that mediates feedback inhibition of lipid synthesis (a pull step). Such push-and-pull engineered microbes exhibit greatly enhanced conversion yields and TAG synthesis and storage properties.

Inventors:

Stephanopoulos, Gregory; Tai, Mitchell; Chakraborty, Sagar

Issue Date:

2018-01-09

Research Org.:

Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1416529

Patent Number(s):

9862977

Application Number:

14/547,304

Assignee:

Massachusetts Institute of Technology (Cambridge, MA)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N1/16 - Yeasts
C12N15/815 - {for yeasts other than Saccharomyces}
C12N9/0083 - {Miscellaneous
C12N9/0097 - {acting on reduced flavodoxin as donor
C12N9/1025 - {Acyltransferases
C12N9/1029 - {transferring groups other than amino-acyl groups
C12N9/88 - Lyases
C12N9/93 - {Ligases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P7/64 - Fats
C12P7/6463 - {obtained from glyceride producing microorganisms, e.g. single cell oil}
C12P7/649 - {Biodiesel, i.e. Fatty acid alkyl esters}

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y114/19001 - Stearoyl-CoA 9-desaturase
C12Y203/03008 - ATP citrate synthase
C12Y604/01002 - Acetyl-CoA carboxylase

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

Y02E50/10 - Biofuels

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DOE Contract Number:  

AR0000059

Resource Type:

Patent

Resource Relation:

Patent File Date: 2014 Nov 19

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS