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Title: Engineered microbes and methods for microbial oil production

Some aspects of this invention provide engineered microbes for oil production. Methods for microbe engineering and for use of engineered microbes are also provided herein. In some embodiments, microbes are provided that are engineered to modulate a combination of rate-controlling steps of lipid synthesis, for example, a combination of a step generating metabolites, acetyl-CoA, ATP or NADPH for lipid synthesis (a push step), and a step sequestering a product or an intermediate of a lipid synthesis pathway that mediates feedback inhibition of lipid synthesis (a pull step). Such push-and-pull engineered microbes exhibit greatly enhanced conversion yields and TAG synthesis and storage properties.
Inventors:
; ;
Issue Date:
OSTI Identifier:
1416529
Assignee:
Massachusetts Institute of Technology (Cambridge, MA) ARPA-E
Patent Number(s):
9,862,977
Application Number:
14/547,304
Contract Number:
AR0000059
Resource Relation:
Patent File Date: 2014 Nov 19
Research Org:
Massachusetts Institute of Technology, Cambridge, MA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS

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