Title: Methods of hydrolyzing a cellulose using halophilic, thermostable and ionic liquids tolerant cellulases

Abstract

The present invention provides for an isolated or recombinant polypeptide comprising an amino acid sequence having at least 70% identity with the amino acid sequence of a Halorhabdus utahensis cellulase, such as Hu-CBH1, wherein said amino acid sequence has a halophilic thermostable and/or thermophilic cellobiohydrolase (CBH) activity. In some embodiments, the polypeptide has a CBH activity that is resistant to up to about 20% of ionic liquids. The present invention also provides for compositions comprising and methods using the isolated or recombinant polypeptide.

Inventors:

Zhang, Tao; Datta, Supratim; Simmons, Blake A.; Rubin, Edward M.

Issue Date:

Tue Jan 09 00:00:00 EST 2018

Research Org.:

Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1416354

Patent Number(s):

9862982

Application Number:

15/164,813

Assignee:

The Regents of the University of California (Oakland, CA); Sandia Corporation (Albuquerque, NM)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N9/2437 - {Cellulases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P19/02 - Monosaccharides
C12P19/14 - produced by the action of a carbohydrase {
C12P2201/00 - Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
C12P2203/00 - Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y302/01091 - Cellulose 1,4-beta-cellobiosidase

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02P - CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS

Y02P20/52 - using catalysts, e.g. selective catalysts

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DOE Contract Number:  

AC02-05CH11231

Resource Type:

Patent

Resource Relation:

Patent File Date: 2016 May 25

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES