Title: Amplification of biological targets via on-chip culture for biosensing

Abstract

The present invention, in part, relates to methods and apparatuses for on-chip amplification and/or detection of various targets, including biological targets and any amplifiable targets. In some examples, the microculture apparatus includes a single-use, normally-closed fluidic valve that is initially maintained in the closed position by a valve element bonded to an adhesive coating. The valve is opened using a magnetic force. The valve element includes a magnetic material or metal. Such apparatuses and methods are useful for in-field or real-time detection of targets, especially in limited resource settings.

Inventors:

Harper, Jason C.; Edwards, Thayne L.; Carson, Bryan; Finley, Melissa; Arndt, William

Issue Date:

2018-01-02

Research Org.:

Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1415449

Patent Number(s):

9857370

Application Number:

14/157,378

Assignee:

National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM)

Patent Classifications (CPCs):

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

F - MECHANICAL ENGINEERING F16 - ENGINEERING ELEMENTS AND UNITS F16K - VALVES

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

B01L2300/0663 - Whole sensors
B01L2300/0816 - Cards, e.g. flat sample carriers usually with flow in two horizontal directions
B01L2300/0887 - Laminated structure
B01L2400/0406 - capillary forces
B01L2400/0633 - with moving parts
B01L3/502738 - {characterised by integrated valves

F - MECHANICAL ENGINEERING F16 - ENGINEERING ELEMENTS AND UNITS F16K - VALVES

F16K2099/0084 - {Chemistry or biology, e.g. "lab-on-a-chip" technology}
F16K31/086 - {the magnet being movable and actuating a second magnet connected to the closing element}
F16K99/003 - {Valves for single use only}
F16K99/0046 - {using magnets}

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/56911 - {Bacteria}

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

Y10T137/87917 - Flow path with serial valves and/or closures

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DOE Contract Number:  

AC04-94AL85000

Resource Type:

Patent

Resource Relation:

Patent File Date: 2014 Jan 16

Country of Publication:

United States

Language:

English

Subject:

47 OTHER INSTRUMENTATION; 59 BASIC BIOLOGICAL SCIENCES