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Title: Recombinant cells and organisms having persistent nonstandard amino acid dependence and methods of making them

Recombinant cells and recombinant organisms persistently expressing nonstandard amino acids (NSAAs) are provided. Methods of making recombinant cells and recombinant organisms dependent on persistently expressing NSAAs for survival are also provided. These methods may be used to make safe recombinant cells and recombinant organisms and/or to provide a selective pressure to maintain one or more reassigned codon functions in recombinant cells and recombinant organisms.
Inventors:
; ;
Issue Date:
OSTI Identifier:
1411431
Assignee:
President and Fellows of Harvard College (Cambridge, MA) CHO
Patent Number(s):
9,834,775
Application Number:
15/025,406
Contract Number:
FG02-02ER63445
Resource Relation:
Patent File Date: 2014 Sep 26
Research Org:
President and Fellows of Harvard College, Cambridge, MA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Works referenced in this record:

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Generation of a Bacterium with a 21 Amino Acid Genetic Code
journal, January 2003
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