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Title: Multiplane optical microscope

This disclosure provides systems, methods, and apparatus related to optical microscopy. In one aspect, an apparatus includes a sample holder, a first objective lens, a plurality of optical components, a second objective lens, and a mirror. The apparatus may directly image a cross-section of a sample oblique to or parallel to the optical axis of the first objective lens, without scanning.
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Issue Date:
OSTI Identifier:
The Regents of the University of California (Oakland, CA) LBNL
Patent Number(s):
Application Number:
Contract Number:
Resource Relation:
Patent File Date: 2015 Jan 07
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
Country of Publication:
United States

Works referenced in this record:

Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates
journal, February 2012
  • Botcherby, E. J.; Smith, C. W.; Kohl, M. M.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 8, p. 2919-2924
  • DOI: 10.1073/pnas.1111662109

Real-time slit scanning microscopy in the meridional plane
journal, January 2009
  • Botcherby, Edward J.; Booth, Martin J.; Juškaitis, Rimas
  • Optics Letters, Vol. 34, Issue 10, p. 1504-1506
  • DOI: 10.1364/OL.34.001504

Optically sectioned imaging by oblique plane microscopy
journal, January 2008

Imaging in high-aperture optical systems
journal, January 1987
  • Sheppard, C. J. R.; Matthews, H. J.
  • Journal of the Optical Society of America A, Vol. 4, Issue 8, p. 1354-1360
  • DOI: 10.1364/JOSAA.4.001354

Resolution of oblique-plane images in sectioning microscopy
journal, January 2011
  • Smith, C. W.; Botcherby, E. J.; Wilson, T.
  • Optics Express, Vol. 19, Issue 3, p. 2662-2669
  • DOI: 10.1364/OE.19.002662

Agitation-free multiphoton microscopy of oblique planes
journal, January 2011
  • Smith, Christopher W.; Botcherby, Edward J.; Booth, Martin J.
  • Optics Letters, Vol. 36, Issue 5, p. 663-665
  • DOI: 10.1364/OL.36.000663

Aberration-free optical refocusing in high numerical aperture microscopy
journal, January 2007
  • Botcherby, Edward J.; Juskaitis, Rimas; Booth, Martin J.
  • Optics Letters, Vol. 32, Issue 14, p. 2007-2009
  • DOI: 10.1364/OL.32.002007

An optical technique for remote focusing in microscopy
journal, February 2008

Three-dimensional imaging and photostimulation by remote-focusing and holographic light patterning
journal, November 2011
  • Anselmi, F.; Ventalon, C.; Begue, A.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 49, p. 19504-19509
  • DOI: 10.1073/pnas.1109111108

Inclined selective plane illumination microscopy adaptor for conventional microscopes
journal, June 2012
  • Cutrale, Francesco; Gratton, Enrico
  • Microscopy Research and Technique, Vol. 75, Issue 11, p. 1461-1466
  • DOI: 10.1002/jemt.22089

Vectorial point spread function and optical transfer function in oblique plane imaging
journal, January 2014
  • Kim, Jeongmin; Li, Tongcang; Wang, Yuan
  • Optics Express, Vol. 22, Issue 9, p. 11140-11151
  • DOI: 10.1364/OE.22.011140

Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy
journal, August 2004

Combined atomic force microscopy and side-view optical imaging for mechanical studies of cells
journal, April 2009
  • Chaudhuri, Ovijit; Parekh, Sapun H.; Lam, Wilbur A.
  • Nature Methods, Vol. 6, Issue 5, p. 383-387
  • DOI: 10.1038/nmeth.1320

High-speed 2D and 3D fluorescence microscopy of cardiac myocytes
journal, January 2011
  • Kumar, Sunil; Wilding, Dean; Sikkel, Markus B.
  • Optics Express, Vol. 19, Issue 15, p. 13839-13847
  • DOI: 10.1364/OE.19.013839

Axial Plane Optical Microscopy
journal, December 2014
  • Li, Tongcang; Ota, Sadao; Kim, Jeongmin
  • Scientific Reports, Vol. 4, Article No. 7253
  • DOI: 10.1038/srep07253

New Angles on Neuronal Dendrites In Vivo
journal, December 2007
  • Göbel, Werner; Helmchen, Fritjof
  • Journal of Neurophysiology, Vol. 98, Issue 6, p. 3770-3779
  • DOI: 10.1152/jn.00850.2007