Methods and apparatus for analysis of chromatographic migration patterns
Abstract
A method and apparatus are presented for sharpening signal peaks in a signal representing the distribution of biological or chemical components of a mixture separated by a chromatographic technique such as, but not limited to, electrophoresis. A key step in the method is the use of a blind deconvolution technique, presently embodied as homomorphic filtering, to reduce the contribution of a blurring function to the signal encoding the peaks of the distribution. The invention further includes steps and apparatus directed to determination of a nucleotide sequence from a set of four such signals representing DNA sequence data derived by electrophoretic means. 16 figures.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Utah, Salt Lake City, UT (United States)
- OSTI Identifier:
- 140713
- Patent Number(s):
- 5273632
- Assignee:
- Univ. of Utah Research Foundation, Salt Lake City, UT (United States)
- DOE Contract Number:
- FG02-88ER60700
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 28 Dec 1993
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; ELECTROPHORESIS; DESIGN; IMAGE PROCESSING; CHROMATOGRAPHY; DNA SEQUENCERS; MEASURING METHODS; TECHNOLOGY ASSESSMENT
Citation Formats
Stockham, T G, and Ives, J T. Methods and apparatus for analysis of chromatographic migration patterns. United States: N. p., 1993.
Web.
Stockham, T G, & Ives, J T. Methods and apparatus for analysis of chromatographic migration patterns. United States.
Stockham, T G, and Ives, J T. Tue .
"Methods and apparatus for analysis of chromatographic migration patterns". United States.
@article{osti_140713,
title = {Methods and apparatus for analysis of chromatographic migration patterns},
author = {Stockham, T G and Ives, J T},
abstractNote = {A method and apparatus are presented for sharpening signal peaks in a signal representing the distribution of biological or chemical components of a mixture separated by a chromatographic technique such as, but not limited to, electrophoresis. A key step in the method is the use of a blind deconvolution technique, presently embodied as homomorphic filtering, to reduce the contribution of a blurring function to the signal encoding the peaks of the distribution. The invention further includes steps and apparatus directed to determination of a nucleotide sequence from a set of four such signals representing DNA sequence data derived by electrophoretic means. 16 figures.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1993},
month = {12}
}