Global to push GA events into
skip to main content

Title: Engineering microorganisms to increase ethanol production by metabolic redirection

The present invention provides for the manipulation of carbon flux in a recombinant host cell to increase the formation of desirable products. The invention relates to cellulose-digesting organisms that have been genetically modified to allow the production of ethanol at a high yield by redirecting carbon flux at key steps of central metabolism.
Inventors:
; ; ; ; ; ; ; ; ;
Issue Date:
OSTI Identifier:
1406573
Assignee:
Enchi Corporation GFO
Patent Number(s):
9,803,221
Application Number:
14/348,231
Contract Number:
FC36-07GO17057; POS2-06ER64304
Resource Relation:
Patent File Date: 2012 Sep 28
Research Org:
Enchi Corporation, Wellesley Hills, MA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS

Works referenced in this record:

Altered regulation of pyruvate kinase or co-overexpression of phosphofructokinase increases glycolytic fluxes in resting Escherichia coli
journal, March 2000

Metabolic engineering of Escherichia coli and Corynebacterium glutamicum for biotechnological production of organic acids and amino acids
journal, June 2006
  • Wendisch, Volker F.; Bott, Michael; Eikmanns, Bernhard J.
  • Current Opinion in Microbiology, Vol. 9, Issue 3, p. 268-274
  • DOI: 10.1016/j.mib.2006.03.001

Coordination of Adenosylmethionine to a Unique Iron Site of the [4Fe-4S] of Pyruvate Formate-Lyase Activating Enzyme:  A Mössbauer Spectroscopic Study
journal, February 2002
  • Krebs, Carsten; Broderick, William E.; Henshaw, Timothy F.
  • Journal of the American Chemical Society, Vol. 124, Issue 6, p. 912-913
  • DOI: 10.1021/ja017562i

Enhanced Hydrogen Production from Formic Acid by Formate Hydrogen Lyase-Overexpressing Escherichia coli Strains
journal, November 2005

Protein tolerance to random amino acid change
journal, June 2004
  • Guo, H. H.; Choe, J.; Loeb, L. A.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 25, p. 9205-9210
  • DOI: 10.1073/pnas.0403255101

Transforming growth factor alpha: mutation of aspartic acid 47 and leucine 48 results in different biological activities.
journal, March 1988
  • Lazar, E.; Watanabe, S.; Dalton, S.
  • Molecular and Cellular Biology, Vol. 8, Issue 3, p. 1247-1252
  • DOI: 10.1128/MCB.8.3.1247

Functional Analysis of Conserved Histidines in ADP-Glucose Pyrophosphorylase from Escherichia coli
journal, March 1998
  • Hill, Margaret A.; Preiss, Jack
  • Biochemical and Biophysical Research Communications, Vol. 244, Issue 2, p. 573-577
  • DOI: 10.1006/bbrc.1998.8301

Fermentation metabolism and its evolution in algae
journal, January 2013
  • Catalanotti, Claudia; Yang, Wenqiang; Posewitz, Matthew C.
  • Frontiers in Plant Science, Vol. 4, Article No. 150
  • DOI: 10.3389%2Ffpls.2013.00150

High Ethanol Titers from Cellulose by Using Metabolically Engineered Thermophilic, Anaerobic Microbes
journal, September 2011
  • Argyros, D. Aaron; Tripathi, Shital A.; Barrett, Trisha F.
  • Applied and Environmental Microbiology, Vol. 77, Issue 23, p. 8288-8294
  • DOI: 10.1128/AEM.00646-11

Metabolic Engineering of Escherichia coli: Increase of NADH Availability by Overexpressing an NAD+-Dependent Formate Dehydrogenase
journal, July 2002
  • Berríos-Rivera, Susana; Bennett, George N.; San, Ka-Yiu
  • Metabolic Engineering, Vol. 4, Issue 3, p. 217-229
  • DOI: 10.1006/mben.2002.0227

Cellulase, Clostridia, and Ethanol
journal, March 2005
  • Demain, A. L.; Newcomb, M.; Wu, J. H. D.
  • Microbiology and Molecular Biology Reviews, Vol. 69, Issue 1, p. 124-154
  • DOI: 10.1128/MMBR.69.1.124-154.2005

Redirecting carbon flux through exogenous pyruvate kinase to achieve high ethanol yields in Clostridium thermocellum
journal, January 2013

Complete Genome Sequence of the Cellulolytic Thermophile Clostridium thermocellum DSM1313
journal, April 2011
  • Feinberg, L.; Foden, J.; Barrett, T.
  • Journal of Bacteriology, Vol. 193, Issue 11, p. 2906-2907
  • DOI: 10.1128/JB.00322-11

A novel NADP+-dependent formate dehydrogenase from Burkholderia stabilis 15516: Screening, purification and characterization
journal, June 2010

Microbial Cellulose Utilization: Fundamentals and Biotechnology
journal, September 2002
  • Lynd, L. R.; Weimer, P. J.; van Zyl, W. H.
  • Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577
  • DOI: 10.1128/MMBR.66.3.506-577.2002

Deletion of the Cel48S cellulase from Clostridium thermocellum
journal, September 2010
  • Olson, Daniel G.; Tripathi, Shital A.; Giannone, Richard J.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 41, p. 17727-17732
  • DOI: 10.1073/pnas.1003584107

Genome-scale metabolic analysis of Clostridium thermocellum for bioethanol production
journal, January 2010
  • Roberts, Seth B.; Gowen, Christopher M.; Brooks, J. Paul
  • BMC Systems Biology, Vol. 4, Issue 1, Article No. 31
  • DOI: 10.1186/1752-0509-4-31

Saccharomyces cerevisiae-Based Molecular Tool Kit for Manipulation of Genes from Gram-Negative Bacteria
journal, July 2006
  • Shanks, Robert; Caiazza, Nicky; Hinsa, Shannon
  • Applied and Environmental Microbiology, Vol. 72, Issue 7, p. 5027-5036
  • DOI: 10.1128/AEM.00682-06

The codon adaptation index-a measure of directional synonymous codon usage bias, and its potential applications
journal, January 1987
  • Sharp, Paul M.; Li, Wen-Hsiung
  • Nucleic Acids Research, Vol. 15, Issue 3, p. 1281-1295
  • DOI: 10.1093/nar/15.3.1281

Metabolic engineering of a thermophilic bacterium to produce ethanol at high yield
journal, September 2008
  • Shaw, A. J.; Podkaminer, K. K.; Desai, S. G.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 37, p. 13769-13774
  • DOI: 10.1073/pnas.0801266105

Development of pyrF-Based Genetic System for Targeted Gene Deletion in Clostridium thermocellum and Creation of a pta Mutant
journal, August 2010
  • Tripathi, S. A.; Olson, D. G.; Argyros, D. A.
  • Applied and Environmental Microbiology, Vol. 76, Issue 19, p. 6591-6599
  • DOI: 10.1128/AEM.01484-10

Atypical Glycolysis in Clostridium thermocellum
journal, February 2013
  • Zhou, Jilai; Olson, Daniel G.; Argyros, D. Aaron
  • Applied and Environmental Microbiology, Vol. 79, Issue 9, p. 3000-3008
  • DOI: 10.1128/AEM.04037-12