Engineering microorganisms to increase ethanol production by metabolic redirection
Abstract
The present invention provides for the manipulation of carbon flux in a recombinant host cell to increase the formation of desirable products. The invention relates to cellulose-digesting organisms that have been genetically modified to allow the production of ethanol at a high yield by redirecting carbon flux at key steps of central metabolism.
- Inventors:
- Issue Date:
- Research Org.:
- Enchi Corporation, Wellesley Hills, MA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1406573
- Patent Number(s):
- 9803221
- Application Number:
- 14/348,231
- Assignee:
- Enchi Corporation
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- FC36-07GO17057; POS2-06ER64304
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2012 Sep 28
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS
Citation Formats
Deng, Yu, Olson, Daniel G., van Dijken, Johannes Pieter, Shaw, IV, Arthur J., Argyros, Aaron, Barrett, Trisha, Caiazza, Nicky, Herring, Christopher D., Rogers, Stephen R., and Agbogbo, Frank. Engineering microorganisms to increase ethanol production by metabolic redirection. United States: N. p., 2017.
Web.
Deng, Yu, Olson, Daniel G., van Dijken, Johannes Pieter, Shaw, IV, Arthur J., Argyros, Aaron, Barrett, Trisha, Caiazza, Nicky, Herring, Christopher D., Rogers, Stephen R., & Agbogbo, Frank. Engineering microorganisms to increase ethanol production by metabolic redirection. United States.
Deng, Yu, Olson, Daniel G., van Dijken, Johannes Pieter, Shaw, IV, Arthur J., Argyros, Aaron, Barrett, Trisha, Caiazza, Nicky, Herring, Christopher D., Rogers, Stephen R., and Agbogbo, Frank. Tue .
"Engineering microorganisms to increase ethanol production by metabolic redirection". United States. https://www.osti.gov/servlets/purl/1406573.
@article{osti_1406573,
title = {Engineering microorganisms to increase ethanol production by metabolic redirection},
author = {Deng, Yu and Olson, Daniel G. and van Dijken, Johannes Pieter and Shaw, IV, Arthur J. and Argyros, Aaron and Barrett, Trisha and Caiazza, Nicky and Herring, Christopher D. and Rogers, Stephen R. and Agbogbo, Frank},
abstractNote = {The present invention provides for the manipulation of carbon flux in a recombinant host cell to increase the formation of desirable products. The invention relates to cellulose-digesting organisms that have been genetically modified to allow the production of ethanol at a high yield by redirecting carbon flux at key steps of central metabolism.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {10}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Recycling system for manipulation of intracellular NADH availability
patent, August 2007
- San, Ka-Yiu; Berrios-Rivera, Susana J.; Bennett, George N.
- US Patent Document 7,256,016
Recycling system for manipulation of intracellular NADH availability
patent, May 2010
- San, Ka-Yiu; Berrios-Rivera, Susan J.; Bennett, George N.
- US Patent Document 7,709,261
High succinate producing bacteria
patent-application, April 2006
- Ka-Yiu, San; Bennett, George N.; Henry, Lin
- US Patent Document 11/228830; 20060073577
Native homoethanol Pathway for ethanol production in E. coli
patent-application, March 2009
- Zhou, Shengde
- US Patent Document 12/156872; 20090082600
Plasmids from Thermophilic Organisms, Vectors Derived Therefrom, and Uses Thereof
patent-application, March 2011
- Caiazza, Nicky; Warner, Anne; Herring, Chris
- US Patent Document 12/677428; 20110059485
Product Recovery From Fermentation of Lignocellulosic Biomass
patent-application, July 2011
- Bardsley, John
- US Patent Document 12/740839; 20110171709
Construction of Protrophic/Celluloytic Yeast Strains Expressing Tethered and Secreted Cellulases
patent-application, August 2011
- Mcbride, John; Lynd, Lee; Deleault, Kristen
- US Patent Document 12/992001; 20110189744
Positive And Negative Selectable Markers For Use In Thermophilic Organisms
patent-application, February 2013
- Tripathi, Shital; Argyros, Aaron; Barrett, Trisha
- US Patent Application 13/393093; 20130052646
Altered regulation of pyruvate kinase or co-overexpression of phosphofructokinase increases glycolytic fluxes in resting Escherichia coli
journal, March 2000
- Emmerling, Marcel; Bailey, James E.; Sauer, Uwe
- Biotechnology and Bioengineering, Vol. 67, Issue 5, p. 623-627
Metabolic engineering of Escherichia coli and Corynebacterium glutamicum for biotechnological production of organic acids and amino acids
journal, June 2006
- Wendisch, Volker F.; Bott, Michael; Eikmanns, Bernhard J.
- Current Opinion in Microbiology, Vol. 9, Issue 3, p. 268-274
Coordination of Adenosylmethionine to a Unique Iron Site of the [4Fe-4S] of Pyruvate Formate-Lyase Activating Enzyme: A Mössbauer Spectroscopic Study
journal, February 2002
- Krebs, Carsten; Broderick, William E.; Henshaw, Timothy F.
- Journal of the American Chemical Society, Vol. 124, Issue 6, p. 912-913
Protein tolerance to random amino acid change
journal, June 2004
- Guo, H. H.; Choe, J.; Loeb, L. A.
- Proceedings of the National Academy of Sciences, Vol. 101, Issue 25, p. 9205-9210
Transforming growth factor alpha: mutation of aspartic acid 47 and leucine 48 results in different biological activities.
journal, March 1988
- Lazar, E.; Watanabe, S.; Dalton, S.
- Molecular and Cellular Biology, Vol. 8, Issue 3, p. 1247-1252
Functional Analysis of Conserved Histidines in ADP-Glucose Pyrophosphorylase from Escherichia coli
journal, March 1998
- Hill, Margaret A.; Preiss, Jack
- Biochemical and Biophysical Research Communications, Vol. 244, Issue 2, p. 573-577
Fermentation metabolism and its evolution in algae
journal, January 2013
- Catalanotti, Claudia; Yang, Wenqiang; Posewitz, Matthew C.
- Frontiers in Plant Science, Vol. 4, Article No. 150
High Ethanol Titers from Cellulose by Using Metabolically Engineered Thermophilic, Anaerobic Microbes
journal, September 2011
- Argyros, D. Aaron; Tripathi, Shital A.; Barrett, Trisha F.
- Applied and Environmental Microbiology, Vol. 77, Issue 23, p. 8288-8294
Metabolic Engineering of Escherichia coli: Increase of NADH Availability by Overexpressing an NAD+-Dependent Formate Dehydrogenase
journal, July 2002
- Berríos-Rivera, Susana; Bennett, George N.; San, Ka-Yiu
- Metabolic Engineering, Vol. 4, Issue 3, p. 217-229
Cellulase, Clostridia, and Ethanol
journal, March 2005
- Demain, A. L.; Newcomb, M.; Wu, J. H. D.
- Microbiology and Molecular Biology Reviews, Vol. 69, Issue 1, p. 124-154
Redirecting carbon flux through exogenous pyruvate kinase to achieve high ethanol yields in Clostridium thermocellum
journal, January 2013
- Deng, Yu; Olson, Daniel G.; Zhou, Jilai
- Metabolic Engineering, Vol. 15, p. 151-158
A novel NADP+-dependent formate dehydrogenase from Burkholderia stabilis 15516: Screening, purification and characterization
journal, June 2010
- Hatrongjit, Rujirat; Packdibamrung, Kanoktip
- Enzyme and Microbial Technology, Vol. 46, Issue 7, p. 557-561
Microbial Cellulose Utilization: Fundamentals and Biotechnology
journal, September 2002
- Lynd, L. R.; Weimer, P. J.; van Zyl, W. H.
- Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577
Deletion of the Cel48S cellulase from Clostridium thermocellum
journal, September 2010
- Olson, Daniel G.; Tripathi, Shital A.; Giannone, Richard J.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 41, p. 17727-17732
Genome-scale metabolic analysis of Clostridium thermocellum for bioethanol production
journal, January 2010
- Roberts, Seth B.; Gowen, Christopher M.; Brooks, J. Paul
- BMC Systems Biology, Vol. 4, Issue 1, Article No. 31
Saccharomyces cerevisiae-Based Molecular Tool Kit for Manipulation of Genes from Gram-Negative Bacteria
journal, July 2006
- Shanks, Robert; Caiazza, Nicky; Hinsa, Shannon
- Applied and Environmental Microbiology, Vol. 72, Issue 7, p. 5027-5036
The codon adaptation index-a measure of directional synonymous codon usage bias, and its potential applications
journal, January 1987
- Sharp, Paul M.; Li, Wen-Hsiung
- Nucleic Acids Research, Vol. 15, Issue 3, p. 1281-1295
Metabolic engineering of a thermophilic bacterium to produce ethanol at high yield
journal, September 2008
- Shaw, A. J.; Podkaminer, K. K.; Desai, S. G.
- Proceedings of the National Academy of Sciences, Vol. 105, Issue 37, p. 13769-13774
Development of pyrF-Based Genetic System for Targeted Gene Deletion in Clostridium thermocellum and Creation of a pta Mutant
journal, August 2010
- Tripathi, S. A.; Olson, D. G.; Argyros, D. A.
- Applied and Environmental Microbiology, Vol. 76, Issue 19, p. 6591-6599
Atypical Glycolysis in Clostridium thermocellum
journal, February 2013
- Zhou, Jilai; Olson, Daniel G.; Argyros, D. Aaron
- Applied and Environmental Microbiology, Vol. 79, Issue 9, p. 3000-3008