Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid

Title: Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid

Embodiments of the present invention are directed toward devices, systems, and methods for purifying nucleic acids to conduct polymerase chain reaction (PCR) assays. In one example, a method includes generating complexes of silica beads and nucleic acids in a lysis buffer, transporting the complexes through an immiscible fluid to remove interfering compounds from the complexes, further transporting the complexes into a density medium containing components required for PCR where the nucleic acids disassociate from the silica beads, and thermocycling the contents of the density medium to achieve PCR. Signal may be detected from labeling agents in the components required for PCR.

Inventors:: Koh, Chung-Yan; Light, Yooli Kim; Piccini, Matthew Ernest; Singh, Anup K.

Issue Date:: 2017-10-31

OSTI Identifier:: 1406570

Assignee:: National Technology & Engineering Solutions of Sandia, LLC SNL-A

Patent Number(s):: 9,803,238

Application Number:: 14/090,040

Contract Number:: AC04-94AL85000

Resource Relation:: Patent File Date: 2013 Nov 26

Research Org:: Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Sponsoring Org:: USDOE

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

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Science, Vol. 301, Issue 5630, p. 213-215
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A polymerase chain reaction system for analyzing a sample containing nucleic acid includes providing magnetic beads; providing a flow channel having a polymerase chain reaction chamber, a pre polymerase chain reaction magnet position adjacent the polymerase chain reaction chamber, and a post pre polymerase magnet position adjacent the polymerase chain reaction chamber. The nucleic acid is bound to the magnetic beads. The magnetic beads with the nucleic acid flow to the pre polymerase chain reaction magnet position in the flow channel. The magnetic beads and the nucleic acid are washed with ethanol. The nucleic acid in the polymerase chain reactionmore »« less
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