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Title: Devices, systems, and methods for detecting nucleic acids using sedimentation

Abstract

Embodiments of the present invention are directed toward devices, systems, and method for conducting nucleic acid purification and quantification using sedimentation. In one example, a method includes generating complexes which bind to a plurality of beads in a fluid sample, individual ones of the complexes comprising a nucleic acid molecule such as DNA or RNA and a labeling agent. The plurality of beads including the complexes may be transported through a density media, wherein the density media has a density lower than a density of the beads and higher than a density of the fluid sample, and wherein the transporting occurs, at least in part, by sedimentation. Signal may be detected from the labeling agents of the complexes.

Inventors:
; ;
Issue Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1404945
Patent Number(s):
9795961
Application Number:
13/423,008
Assignee:
National Technology & Engineering Solutions of Sandia, LLC
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Mar 16
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION

Citation Formats

Koh, Chung-Yan, Schaff, Ulrich Y., and Sommer, Gregory J. Devices, systems, and methods for detecting nucleic acids using sedimentation. United States: N. p., 2017. Web.
Koh, Chung-Yan, Schaff, Ulrich Y., & Sommer, Gregory J. Devices, systems, and methods for detecting nucleic acids using sedimentation. United States.
Koh, Chung-Yan, Schaff, Ulrich Y., and Sommer, Gregory J. Tue . "Devices, systems, and methods for detecting nucleic acids using sedimentation". United States. https://www.osti.gov/servlets/purl/1404945.
@article{osti_1404945,
title = {Devices, systems, and methods for detecting nucleic acids using sedimentation},
author = {Koh, Chung-Yan and Schaff, Ulrich Y. and Sommer, Gregory J.},
abstractNote = {Embodiments of the present invention are directed toward devices, systems, and method for conducting nucleic acid purification and quantification using sedimentation. In one example, a method includes generating complexes which bind to a plurality of beads in a fluid sample, individual ones of the complexes comprising a nucleic acid molecule such as DNA or RNA and a labeling agent. The plurality of beads including the complexes may be transported through a density media, wherein the density media has a density lower than a density of the beads and higher than a density of the fluid sample, and wherein the transporting occurs, at least in part, by sedimentation. Signal may be detected from the labeling agents of the complexes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {10}
}

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