Devices, systems, and methods for detecting nucleic acids using sedimentation
Abstract
Embodiments of the present invention are directed toward devices, systems, and method for conducting nucleic acid purification and quantification using sedimentation. In one example, a method includes generating complexes which bind to a plurality of beads in a fluid sample, individual ones of the complexes comprising a nucleic acid molecule such as DNA or RNA and a labeling agent. The plurality of beads including the complexes may be transported through a density media, wherein the density media has a density lower than a density of the beads and higher than a density of the fluid sample, and wherein the transporting occurs, at least in part, by sedimentation. Signal may be detected from the labeling agents of the complexes.
- Inventors:
- Issue Date:
- Research Org.:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1404945
- Patent Number(s):
- 9795961
- Application Number:
- 13/423,008
- Assignee:
- National Technology & Engineering Solutions of Sandia, LLC
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- AC04-94AL85000
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2012 Mar 16
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION
Citation Formats
Koh, Chung-Yan, Schaff, Ulrich Y., and Sommer, Gregory J. Devices, systems, and methods for detecting nucleic acids using sedimentation. United States: N. p., 2017.
Web.
Koh, Chung-Yan, Schaff, Ulrich Y., & Sommer, Gregory J. Devices, systems, and methods for detecting nucleic acids using sedimentation. United States.
Koh, Chung-Yan, Schaff, Ulrich Y., and Sommer, Gregory J. Tue .
"Devices, systems, and methods for detecting nucleic acids using sedimentation". United States. https://www.osti.gov/servlets/purl/1404945.
@article{osti_1404945,
title = {Devices, systems, and methods for detecting nucleic acids using sedimentation},
author = {Koh, Chung-Yan and Schaff, Ulrich Y. and Sommer, Gregory J.},
abstractNote = {Embodiments of the present invention are directed toward devices, systems, and method for conducting nucleic acid purification and quantification using sedimentation. In one example, a method includes generating complexes which bind to a plurality of beads in a fluid sample, individual ones of the complexes comprising a nucleic acid molecule such as DNA or RNA and a labeling agent. The plurality of beads including the complexes may be transported through a density media, wherein the density media has a density lower than a density of the beads and higher than a density of the fluid sample, and wherein the transporting occurs, at least in part, by sedimentation. Signal may be detected from the labeling agents of the complexes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {10}
}
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