Enzymes that cleave non-glycosidic ether bonds between lignins or derivatives thereof and saccharides

Kravit, Nancy G.; Schmidt, Katherine A.

Title: Enzymes that cleave non-glycosidic ether bonds between lignins or derivatives thereof and saccharides

Full Record
Other Related Research

Abstract

The patent application relates to isolated polypeptides that specifically cleave non-glycosidic ether bonds between lignins or derivatives thereof and saccharides, and to cDNAs encoding the polypeptides. The patent application also relates to nucleic acid constructs, expression vectors and host cells comprising the cDNAs, as well as methods of producing and using the isolated polypeptides for treating pulp and biomass to increase soluble saccharide yield and enrich lignin fractions.

Inventors:: Kravit, Nancy G.; Schmidt, Katherine A.

Issue Date:: 2017-10-24

Research Org.:: Tethys Research LLC, Bethesda, MD (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1404941

Patent Number(s):: 9796969

Application Number:: 14/747,606

Assignee:: Tethys Research LLC (Bethesda, MD)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

G - PHYSICS G07 - CHECKING-DEVICES G07G - REGISTERING THE RECEIPT OF CASH, VALUABLES, OR TOKENS

Show more

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C12Q1/527 - involving lyase

G - PHYSICS G07 - CHECKING-DEVICES G07G - REGISTERING THE RECEIPT OF CASH, VALUABLES, OR TOKENS
G07G1/00 - Cash registers

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P1/00 - Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07G - COMPOUNDS OF UNKNOWN CONSTITUTION
C07G1/00 - Lignin

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/88 - Lyases

D - TEXTILES D21 - PAPER-MAKING D21C - PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS
D21C5/005 - {Treatment of cellulose-containing material with microorganisms or enzymes}

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y402/02 - acting on polysaccharides

Show less

DOE Contract Number:: FG02-07ER84788

Resource Type:: Patent

Resource Relation:: Patent File Date: 2015 Jun 23

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Kravit, Nancy G., and Schmidt, Katherine A. Enzymes that cleave non-glycosidic ether bonds between lignins or derivatives thereof and saccharides.  United States: N. p., 2017. 
        Web.

Copy to clipboard


                    Kravit, Nancy G., & Schmidt, Katherine A. Enzymes that cleave non-glycosidic ether bonds between lignins or derivatives thereof and saccharides.  United States.

Copy to clipboard


                    Kravit, Nancy G., and Schmidt, Katherine A. Tue .  
        "Enzymes that cleave non-glycosidic ether bonds between lignins or derivatives thereof and saccharides".  United States.  https://www.osti.gov/servlets/purl/1404941.

Copy to clipboard


                    
@article{osti_1404941,

  title        = {Enzymes that cleave non-glycosidic ether bonds between lignins or derivatives thereof and saccharides},

  author       = {Kravit, Nancy G. and Schmidt, Katherine A.},

  abstractNote = {The patent application relates to isolated polypeptides that specifically cleave non-glycosidic ether bonds between lignins or derivatives thereof and saccharides, and to cDNAs encoding the polypeptides. The patent application also relates to nucleic acid constructs, expression vectors and host cells comprising the cDNAs, as well as methods of producing and using the isolated polypeptides for treating pulp and biomass to increase soluble saccharide yield and enrich lignin fractions.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2017},

  month        = {10}

}

Copy to clipboard

Patent:

Save / Share:

Export Metadata

Save to My Library

Similar Records in DOE Patents and OSTI.GOV collections:

Termite enzymes and uses thereof for in vitro conversion of lignin-containing materials to fermentable products

Patent Scharf, Michael E; Boucias, Drion G; Tartar, Aurelien; ...

The disclosure provides isolated nucleic acid molecules derived from the gut of the termite R flavipes, recombinant nucleic acid molecules comprising a vector and an isolated heterologous nucleic acid molecule operably inserted therein, whereby, when transformed into an appropriate host cell system, the heterologous nucleic acid sequence is expressed as a polypeptide having an activity similar to that when expressed in the gut of the termite R. flavipes. The recombinant nucleic acid molecules can comprise more than one heterologous nucleic acid molecule such that more than one polypeptide may be expressed by the host system. The expressed polypeptides may bemore » « less
Full Text Available
Methods and systems to produce lignin-modifying enzymes and uses thereof

Patent Khlystov, Nikita A.; Sattely, Elizabeth

Embodiments of the invention are generally directed to lignin-modifying enzymes and systems and methods of their manufacture. In many embodiments, the lignin-modifying enzymes are lignin-degrading enzymes capable of breaking down lignin into component parts that are usable for other purposes. Several embodiments are directed to systems for producing lignin-modifying enzymes in vivo, including in yeast and/or plant species, and certain embodiments are directed to methods of creating these systems, including transfecting the species to produce lignin-modifying enzymes.
Full Text Available
Chimeric enzymes for conversion of lignin-derived chemicals

Patent Neidle, Ellen Lee; Johnson, Christopher W.; Beckham, Gregg Tyler; ...

Disclosed herein are enzymes useful for the dealkylation of aromatic substrates, including the conversion of guaiacol or guaethol to catechol. Methods of converting aromatic substrates found in lignin-based feedstocks such as pyrolysis oil into products such as catechol are also disclosed. Also presented herein are methods for rapidly evolving and optimizing genetic regions.
Full Text Available
Enzyme linked immunoassay with stabilized polymer saccharide enzyme conjugates

Patent Callstrom, Matthew R [Columbus, OH]; Bednarski, Mark D [Berkeley, CA]; Gruber, Patrick R [St. Paul, MN]

An improvement in enzyme linked immunoassays is disclosed wherein the enzyme is in the form of a water soluble polymer saccharide conjugate which is stable in hostile environments. The conjugate comprises the enzyme which is linked to the polymer at multiple points through saccharide linker groups.
Full Text Available
System and method for combustion of non-gaseous fuels and derivatives thereof

Patent Fetvedt, Jeremy Eron; Lu, Xijia; Gregory, Brent Allan; ...

The present disclosure relates to apparatuses and methods that are useful for one or more aspects of a power production plant. More particularly, the disclosure relates to combustor apparatuses and methods for a combustor adapted to utilize different fuel mixtures derived from gasification of a solid fuel. Combustion of the different fuel mixtures within the combustor can be facilitated by arranging elements of the combustor controlled so that a defined set of combustion characteristics remains substantially constant across a range of different fuel mixtures.
Full Text Available

Similar Records