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Title: Unnatural reactive amino acid genetic code additions

This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.
Inventors:
; ; ; ;
Issue Date:
OSTI Identifier:
1404921
Assignee:
The Scripps Research Institute OAK
Patent Number(s):
9,797,908
Application Number:
14/338,113
Contract Number:
FG03-00ER45812
Resource Relation:
Patent File Date: 2014 Jul 22
Research Org:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Other works cited in this record:

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Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs
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Unnatural reactive amino acid genetic code additions
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Expanding the eukaryotic genetic code
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Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
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Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
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Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
patent-application, October 2006

Composition of orthogonal glutamyl-trna and aminoacyl-trna synthetase pairs and uses thereof
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Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof
patent-application, February 2007

Cannabinoid receptor modulator
patent-application, May 2007

Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
patent-application, May 2007

Expanding the eukaryotic genetic code
patent-application, July 2007

Expanding the eukaryotic genetic code
patent-application, July 2007

Glycoprotein synthesis
patent-application, July 2007

Glycoprotein synthesis
patent-application, August 2007

Site specific incorporation of keto amino acids into proteins
patent-application, September 2008

Synthesis and Photophysical Properties of Fluorescent Derivatives of Methylmercury
journal, January 1996
  • Bolletta, Fabrizio; Fabbri, Daniele; Lombardo, Marco
  • Organometallics, Vol. 15, Issue 9, p. 2415-2417
  • DOI: 10.1021/om950793b

In Vivo Photocrosslinking with Unnatural Amino Acid Mutagenesis
journal, November 2002

Addition of p-Azido-L-phenylalanine to the Genetic Code of Escherichia coli
journal, July 2002
  • Chin, Jason W.; Santoro, Stephen W.; Martin, Andrew B.
  • Journal of the American Chemical Society, Vol. 124, Issue 31, p. 9026-9027
  • DOI: 10.1021/ja027007w

Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli
journal, August 2002
  • Chin, J. W.; Martin, A. B.; King, D. S.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 17, p. 11020-11024
  • DOI: 10.1073/pnas.172226299

An Expanded Eukaryotic Genetic Code
journal, August 2003
  • Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher
  • Science, Vol. 301, Issue 5635, p. 964-967
  • DOI: 10.1126/science.1084772

Site-Specific Protein Modification Using a Ketone Handle
journal, January 1996
  • Cornish, Virginia W.; Hahn, Klaus M.; Schultz, Peter G.
  • Journal of the American Chemical Society, Vol. 118, Issue 34, p. 8150-8151
  • DOI: 10.1021/ja961216x

Adding Amino Acids with Novel Reactivity to the Genetic Code of Saccharomyces Cerevisiae
journal, October 2003
  • Deiters, Alexander; Cropp, T. Ashton; Mukherji, Mridul
  • Journal of the American Chemical Society, Vol. 125, Issue 39, p. 11782-11783
  • DOI: 10.1021/ja0370037

A bacterial amber suppressor in Saccharomyces cerevisiae is selectively recognized by a bacterial aminoacyl-tRNA synthetase.
journal, April 1990
  • Edwards, H.; Schimmel, P.
  • Molecular and Cellular Biology, Vol. 10, Issue 4, p. 1633-1641
  • DOI: 10.1128/MCB.10.4.1633

Partition of tRNA synthetases into two classes based on mutually exclusive sets of sequence motifs
journal, September 1990
  • Eriani, Gilbert; Delarue, Marc; Poch, Olivier
  • Nature, Vol. 347, Issue 6289, p. 203-206
  • DOI: 10.1038/347203a0

Expanding tRNA recognition of a tRNA synthetase by a single amino acid change
journal, May 2003
  • Feng, L.; Tumbula-Hansen, D.; Toogood, H.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 10, p. 5676-5681
  • DOI: 10.1073/pnas.0631525100

Programming peptidomimetic syntheses by translating genetic codes designed de novo
journal, May 2003
  • Forster, A. C.; Tan, Z.; Nalam, M. N. L.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 11, p. 6353-6357
  • DOI: 10.1073/pnas.1132122100

Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells
journal, July 1998

A Mutant Escherichia coli Tyrosyl-tRNA Synthetase Utilizes the Unnatural Amino Acid Azatyrosine More Efficiently than Tyrosine
journal, September 2000
  • Hamano-Takaku, Fumie; Iwama, Toshiharu; Saito-Yano, Shio
  • Journal of Biological Chemistry, Vol. 275, Issue 51, p. 40324-40328
  • DOI: 10.1074/jbc.M003696200

Incorporation of azides into recombinant proteins for chemoselective modification by the Staudinger ligation
journal, December 2001
  • Kiick, K. L.; Saxon, E.; Tirrell, D. A.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 1, p. 19-24
  • DOI: 10.1073/pnas.012583299

Import of amber and ochre suppressor tRNAs into mammalian cells: A general approach to site-specific insertion of amino acid analogues into proteins
journal, November 2001
  • Kohrer, C.; Xie, L.; Kellerer, S.
  • Proceedings of the National Academy of Sciences, Vol. 98, Issue 25, p. 14310-14315
  • DOI: 10.1073/pnas.251438898

Click Chemistry: Diverse Chemical Function from a Few Good Reactions
journal, June 2001

Click Chemistry In Situ: Acetylcholinesterase as a Reaction Vessel for the Selective Assembly of a Femtomolar Inhibitor from an Array of Building Blocks
journal, March 2002

Progress toward the evolution of an organism with an expanded genetic code
journal, April 1999
  • Liu, David R.; Schultz, Peter G.
  • Proceedings of the National Academy of Sciences, Vol. 96, Issue 9, p. 4780-4785
  • DOI: 10.1073/pnas.96.9.4780

Engineering Chemical Reactivity on Cell Surfaces Through Oligosaccharide Biosynthesis
journal, May 1997

Activity-Based Protein Profiling in Vivo Using a Copper(I)-Catalyzed Azide-Alkyne [3 + 2] Cycloaddition
journal, April 2003
  • Speers, Anna E.; Adam, Gregory C.; Cravatt, Benjamin F.
  • Journal of the American Chemical Society, Vol. 125, Issue 16, p. 4686-4687
  • DOI: 10.1021/ja034490h

Peptidotriazoles on Solid Phase  [1,2,3]-Triazoles by Regiospecific Copper(I)-Catalyzed 1,3-Dipolar Cycloadditions of Terminal Alkynes to Azides
journal, May 2002
  • Tornøe, Christian W.; Christensen, Caspar; Meldal, Morten
  • The Journal of Organic Chemistry, Vol. 67, Issue 9, p. 3057-3064
  • DOI: 10.1021/jo011148j

Expanding the Genetic Code of Escherichia coli
journal, April 2001

Adding l-3-(2-Naphthyl)alanine to the Genetic Code of E. coli
journal, March 2002
  • Wang, Lei; Brock, Ansgar; Schultz, Peter G.
  • Journal of the American Chemical Society, Vol. 124, Issue 9, p. 1836-1837
  • DOI: 10.1021/ja012307j

Addition of the keto functional group to the genetic code of Escherichia coli
journal, December 2002
  • Wang, L.; Zhang, Z.; Brock, A.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 1, p. 56-61
  • DOI: 10.1073/pnas.0234824100

Bioconjugation by Copper(I)-Catalyzed Azide-Alkyne [3 + 2] Cycloaddition
journal, March 2003
  • Wang, Qian; Chan, Timothy R.; Hilgraf, Robert
  • Journal of the American Chemical Society, Vol. 125, Issue 11, p. 3192-3193
  • DOI: 10.1021/ja021381e

The Selective Incorporation of Alkenes into Proteins in Escherichia coli
journal, August 2002

A New Strategy for the Site-Specific Modification of Proteins in Vivo
journal, June 2003
  • Zhang, Zhiwen; Smith, Brian A. C.; Wang, Lei
  • Biochemistry, Vol. 42, Issue 22, p. 6735-6746
  • DOI: 10.1021/bi0300231

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