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Title: Site-specific incorporation of redox active amino acids into proteins

Abstract

Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate redox active amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with redox active amino acids using these orthogonal pairs.

Inventors:
; ;
Issue Date:
Research Org.:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1398985
Patent Number(s):
9783798
Application Number:
13/344,436
Assignee:
The Scripps Research Institute
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
FG03-00ER45812
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Jan 05
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Alfonta, Lital, Schultz, Peter G., and Zhang, Zhiwen. Site-specific incorporation of redox active amino acids into proteins. United States: N. p., 2017. Web.
Alfonta, Lital, Schultz, Peter G., & Zhang, Zhiwen. Site-specific incorporation of redox active amino acids into proteins. United States.
Alfonta, Lital, Schultz, Peter G., and Zhang, Zhiwen. Tue . "Site-specific incorporation of redox active amino acids into proteins". United States. https://www.osti.gov/servlets/purl/1398985.
@article{osti_1398985,
title = {Site-specific incorporation of redox active amino acids into proteins},
author = {Alfonta, Lital and Schultz, Peter G. and Zhang, Zhiwen},
abstractNote = {Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate redox active amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with redox active amino acids using these orthogonal pairs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {10}
}

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Works referenced in this record:

Site-Specific Incorporation of a Redox-Active Amino Acid into Proteins
journal, December 2003


An Expanded Eukaryotic Genetic Code
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Expanding tRNA recognition of a tRNA synthetase by a single amino acid change
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Programming peptidomimetic syntheses by translating genetic codes designed de novo
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Twenty-first aminoacyl-tRNA synthetase-suppressor tRNA pairs for possible use in site-specific incorporation of amino acid analogues into proteins in eukaryotes and in eubacteria
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A New Functional Suppressor tRNA/Aminoacyl−tRNA Synthetase Pair for the in Vivo Incorporation of Unnatural Amino Acids into Proteins
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Expanding the Genetic Code of Escherichia coli
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The Selective Incorporation of Alkenes into Proteins in Escherichia coli
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A New Strategy for the Site-Specific Modification of Proteins in Vivo
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A New Orthogonal Suppressor tRNA/Aminoacyl-tRNA Synthetase Pair for Evolving an Organism with an Expanded Genetic Code
journal, September 2000


Adding l-3-(2-Naphthyl)alanine to the Genetic Code of E. coli
journal, March 2002