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Title: Site-specific incorporation of redox active amino acids into proteins

Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate redox active amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with redox active amino acids using these orthogonal pairs.
Inventors:
; ;
Issue Date:
OSTI Identifier:
1398985
Assignee:
The Scripps Research Institute CHO
Patent Number(s):
9,783,798
Application Number:
13/344,436
Contract Number:
FG03-00ER45812
Resource Relation:
Patent File Date: 2012 Jan 05
Research Org:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

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In vivo incorporation of unnatural amino acids
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Methods and compositions for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
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Site-Specific Incorporation of a Redox-Active Amino Acid into Proteins
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