System and method for detecting components of a mixture including a valving scheme for competition assays
Abstract
Examples are described including measurement systems for conducting competition assays. A first chamber of an assay device may be loaded with a sample containing a target antigen. The target antigen in the sample may be allowed to bind to antibody-coated beads in the first chamber. A control layer separating the first chamber from a second chamber may then be opened to allow a labeling agent loaded in a first portion of the second chamber to bind to any unoccupied sites on the antibodies. A centrifugal force may then be applied to transport the beads through a density media to a detection region for measurement by a detection unit.
- Inventors:
- Issue Date:
- Research Org.:
- Sandia National Lab. (SNL-CA), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA)
- OSTI Identifier:
- 1393511
- Patent Number(s):
- 9766230
- Application Number:
- 15/616,740
- Assignee:
- National Technology & Engineering Solutions of Sandia, LLC
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- NA0003525
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2017 Jun 07
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 42 ENGINEERING; 59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION
Citation Formats
Koh, Chung-Yan, Piccini, Matthew E., and Singh, Anup K. System and method for detecting components of a mixture including a valving scheme for competition assays. United States: N. p., 2017.
Web.
Koh, Chung-Yan, Piccini, Matthew E., & Singh, Anup K. System and method for detecting components of a mixture including a valving scheme for competition assays. United States.
Koh, Chung-Yan, Piccini, Matthew E., and Singh, Anup K. Tue .
"System and method for detecting components of a mixture including a valving scheme for competition assays". United States. https://www.osti.gov/servlets/purl/1393511.
@article{osti_1393511,
title = {System and method for detecting components of a mixture including a valving scheme for competition assays},
author = {Koh, Chung-Yan and Piccini, Matthew E. and Singh, Anup K.},
abstractNote = {Examples are described including measurement systems for conducting competition assays. A first chamber of an assay device may be loaded with a sample containing a target antigen. The target antigen in the sample may be allowed to bind to antibody-coated beads in the first chamber. A control layer separating the first chamber from a second chamber may then be opened to allow a labeling agent loaded in a first portion of the second chamber to bind to any unoccupied sites on the antibodies. A centrifugal force may then be applied to transport the beads through a density media to a detection region for measurement by a detection unit.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Sep 19 00:00:00 EDT 2017},
month = {Tue Sep 19 00:00:00 EDT 2017}
}
Works referenced in this record:
Devices and Method For Using Centripetal Acceleration To Drive Fluid Movement In A Microfluidics System With On-Board Informatics
patent-application, December 2001
- Mian, Alec; Kieffer-Higgins, Stephen G.; Corey, George D.
- US Patent Document 08/761063; 20010055812
Centrifugal force based microfluidic device, microfluidic system including the same, and method of determining home position of the microfluidic device
patent, July 2010
- Lee, Jeong-gun; Lee, Beom-seok; Park, Jong-myeon
- US Patent Document 7,758,810
Analytical methods and compositions
patent-application, November 2002
- Rao, Galla Chandra; Terstapper, Leon Wmm
- US Patent Application 10/017437; 20020164659
Microfluidic particle-analysis systems
patent-application, April 2004
- Chou, Hou-Pu; Daridon, Antoine; Farrell, Kevin
- US Patent Application 10/405953; 20040072278
Method of separation employing magnetic particles and second medium
patent, January 1994
- Vorpahl, John
- US Patent Document 5,279,936
Optical bio-discs and fluidic circuits for analysis of cells and methods relating thereto
patent, January 2007
- Valencia, Ramoncito M.; Chen, YihFar; Hurt, Susan
- US Patent Document 7,157,049
On-Chip Coupling of Isoelectric Focusing and Free Solution Electrophoresis for Multidimensional Separations
journal, March 2003
- Herr, Amy E.; Molho, Joshua I.; Drouvalakis, Katerina A.
- Analytical Chemistry, Vol. 75, Issue 5, p. 1180-1187
Multistation, Single Channel Analytical Photometer and Method of Use
patent, January 1971
- Anderson, Norman G.
- US Patent Document 3,555,284
Centrifuge with removable core for scalable centrifugation
patent-application, September 2005
- Merino, Sandra Patricia; Dalessio, Steven J.; Rudy Otten, Robin Roy Louis
- US Patent Application 11/129116; 20050215410
Capillary columns with in situ formed porous monolithic packing for micro high-performance liquid chromatography and capillary electrochromatography
journal, September 1999
- Gusev, Isabelle; Huang, Xian; Horváth, Csaba
- Journal of Chromatography A, Vol. 855, Issue 1, p. 273-290
Method and apparatus for measuring blood constituent counts
patent, July 1987
- Wardlaw, Stephen C.
- US Patent Document 4,683,579
Microfluidic devices, systems, and methods for quantifying particles using centrifugal force
patent, November 2015
- Schaff, Ulrich Y.; Sommer, Gregory J.; Singh, Anup K.
- US Patent Document 9,186,668
Diagnostic Analyzers With Pretreatment Carousels and Related Methods
patent-application, September 2014
- Ochranek, Brian L.; Arnquist, David C.
- US Patent Application 14/142474; 20140273241
Rapid isoelectric trapping in a micropreparative-scale multicompartment electrolyzer
journal, June 2007
- Lim, Peniel; North, Robert; Vigh, Gyula
- ELECTROPHORESIS, Vol. 28, Issue 12, p. 1851-1859
Diffusion-based system and method for detecting and monitoring activity of biologic and chemical species
patent-application, October 2003
- Hefti, John
- US Patent Application 10/064392; 20030203504
A Method for Global Analysis of Complex Proteomes Using Sample Prefractionation by Solution Isoelectrofocusing Prior to Two-Dimensional Electrophoresis
journal, September 2000
- Zuo, Xun; Speicher, David W.
- Analytical Biochemistry, Vol. 284, Issue 2
Protecting agent
patent-application, August 2005
- Kange, Rickard; Inganas, Mats; Gustafsson, Magnus
- US Patent Application 11/038712; 20050186685
Method for detecting analytes in a sample
patent-application, December 2009
- Hinnah, Silke Christine; Lambru, Dagmar; Droge, Sonja
- US Patent Application 11/629107; 20090325186
Centrifugation Apparatus For Containers Of Biological Material
patent-application, February 2011
- Pedrazzini, Gianandrea
- US Patent Application 12/867345; 20110045958
Plastic microfluidics enabling two-dimensional protein separations in proteome analysis
patent-application, November 2002
- Lee, Cheng Sheng; DeVoe, Don
- US Patent Application 10/135386; 20020170825
Shiga toxin 1Â acting on DNA in vitro is a heat-stable enzyme not requiring proteolytic activation
journal, April 2004
- Brigotti, Maurizio; Carnicelli, Domenica; Vara, Antonio González
- Biochimie, Vol. 86, Issue 4-5, p. 305-309
Separation of solid and liquid components of mixtures
patent, November 1978
- Hunter, William M.
- US Patent Document 4,125,375
Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation
patent, February 2015
- Schaff, Ulrich Y.; Koh, Chung-Yan; Sommer, Gregory J.
- US Patent Document 8,962,346
Compact disk based platform for separating and detecting immunomagnetic bead labeled cells
patent-application, June 2010
- Wo, Andrew M.; Chen, Chen-Lin; Chen, Ken-Chao
- US Patent Application12/382844; 20100151560
Density-based cell detection system
patent, March 2010
- Kirakossian, Hrair
- US Patent Application 12/380877; 20100068754
Multichannel control in microfluidics
patent-application, October 2002
- Sassi, Alexander; Paulus, Aran
- US Patent Application 10/121378; 20020153251
Microparticle-based methods and systems and applications thereof
patent-application, December 2005
- Prober, James M.; Cui, Xiumin; Dam, Rudy J.
- US Patent Document 11/189080; 20050282220
Heterogeneous binding assay
patent, April 1987
- Baker, Terence Seward; Abbott, Stephen R.; Simpson, John G.
- US Patent Document 4,656,143
Centripetally-motivated microfluidics system for performing in vitro hybridization and amplification of nucleic acids
patent, February 2008
- Kellogg, Gregory John; Carvalho, Bruce L.; Sheppard, Jr., Norman F.
- US Patent Document 7,332,326
Microfluidics devices and methods for performing cell based assays
patent-application, August 2002
- Carvalho, Bruce L.; Sheppard, Norman F. JR.; Feakes, Christina
- US Patent Application 09/858558; 20020106786
Centrifugal Separation System
patent-application, August 2009
- Andersson, Lars
- US Patent Application 12/296982; 20090209402
Ricin A-Chain Activity on Stem−Loop and Unstructured DNA Substrates
journal, March 2005
- Amukele, Tim K.; Roday, Setu; Schramm, Vern L.
- Biochemistry, Vol. 44, Issue 11, p. 4416-4425
Monolithic microfluidic concentrators and mixers
patent, May 2005
- Frechet, Jean M. J.; Svec, Frantisek; Yu, Cong
- US Patent Document 6,887,384
Measurement of Circulating Cell-Free DNA Levels by a New Simple Fluorescent Test in Patients With Primary Colorectal Cancer
journal, February 2011
- Czeiger, David; Shaked, Gad; Eini, Hadar
- American Journal of Clinical Pathology, Vol. 135, Issue 2, p. 264-270
A molecular approach to bioseparations: Protein–protein and protein–salt interactions
journal, February 2006
- Curtis, R. A.; Lue, L.
- Chemical Engineering Science, Vol. 61, Issue 3, p. 907-923
Functional integration of DNA purification and concentration into a real time micro-PCR chip
journal, January 2011
- Min, Junhong; Kim, Joon-Ho; Lee, Youngsun
- Lab on a Chip, Vol. 11, Issue 2, p. 259-265
Parallel Nanoliter Microfluidic Analysis System
journal, June 2007
- Andersson, Per; Jesson, Gerald; Kylberg, Gunnar
- Analytical Chemistry, Vol. 79, Issue 11, p. 4022-4030
Microfluidic particle-analysis systems
patent, December 2007
- Chou, Hou-Pu; Daridon, Antoine; Farrell, Kevin
- US Patent Document 7,312,085
Detecting Single Stranded DNA with a Solid State Nanopore
journal, October 2005
- Fologea, Daniel; Gershow, Marc; Ledden, Bradley
- Nano Letters, Vol. 5, Issue 10, p. 1905-1909
Centrifugal hematology disposable
patent, November 2000
- Thomas, Bradley S.
- US Patent Document 6,153,148
Method for Separating Cells
patent-application, May 2008
- Woodside, Steven M.
- US Patent Application 11/940451; 20080108047
Toxin activity assays, devices, methods and systems therefor
patent, April 2016
- Koh, Chung-Yan; Schaff, Ulrich Y.; Sommer, Gregory J.
- US Patent Document 9,304,128
Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation
patent, April 2016
- Schaff, Ulrich Y.; Koh, Chung-Yan; Sommer, Gregory J.
- US Patent Document 9,304,129
Method For Isolating Nucleic Acids Comprising The Use Of Ethylene Glycol Multimers
patent-application, March 2009
- Finne, Erling
- US Patent Application 11/815967; 20090069554
Pre-column for preconcentrating substances to be chromatographed as well as device for a liquid chromatography system and coupling member for said device
patent, November 1985
- Ruijten, Henri M.; De Bree, Hans; Van Amsterdam, Peter H.
- US Patent Document 4,554,071
Assay system and method for conducting assays
patent, March 1999
- Andrevski, Zygmunt M.; Roach, William R.; Southgate, Peter D.
- US Patent Document 5,882,903
Method and Apparatus For Precise Transfer and Manipulation Of Fluids By Centrifugal and Or Capillary Forces
patent-application, January 2009
- Pugia, Michael J.; Blankenstein, Gert; Peters, Ralf-Peter
- US Patent Document 12/205965; 20090004059
Cell-Free DNA—A Marker to Predict Ischemic Brain Damage in a Rat Stroke Experimental Model
journal, January 2011
- Boyko, Matthew; Ohayon, Sharon; Goldsmith, Tomer
- Journal of Neurosurgical Anesthesiology, Vol. 23, Issue 3, p. 222-228
Multistage Isoelectric Focusing in a Polymeric Microfluidic Chip
journal, December 2005
- Cui, Huanchun; Horiuchi, Keisuke; Dutta, Prashanta
- Analytical Chemistry, Vol. 77, Issue 24, p. 7878-7886
Detection of Listeria monocytogenes and the toxin listeriolysin O in food
journal, February 2006
- Churchill, Robin L. T.; Lee, Hung; Hall, J. Christopher
- Journal of Microbiological Methods, Vol. 64, Issue 2, p. 141-170
Method Of Detecting Analyte-Molecule Interactions
patent-application, June 2008
- Tolley, Luke; McCarroll, Matt
- US Patent Application 11/923079; 20080149484
Formation of Natural pH Gradients in a Microfluidic Device under Flow Conditions:Â Model and Experimental Validation
journal, February 2001
- Cabrera, Catherine R.; Finlayson, Bruce; Yager, Paul
- Analytical Chemistry, Vol. 73, Issue 3, p. 658-666
Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system
patent, November 2001
- Mian, Alec; Kieffer-Higgins, Stephen; Corey, George
- US Patent Document 6,319,469
Systems, devices, and methods for agglutination assays using sedimentation
patent, January 2016
- Schaff, Ulrich Y.; Sommer, Gregory J.; Singh, Anup K.
- US Patent Document 9,244,065
Microfluidic devices and methods
patent-application, January 2003
- Jedrzejewski, Paul; Nock, Steffen; Wagner, Peter
- US Patent Application 10/208381; 20030013203
Systems, Devices, and Methods For Agglutination Assays Using Sedimentation
patent-application, March 2016
- Schaff, Ulrich Y.; Sommer, Gregory Jon; Singh, Anup K.
- US Patent Application14/853660; 20160061829
Infrared controlled waxes for liquid handling and storage on a CD-microfluidic platform
journal, January 2011
- Abi-Samra, Kameel; Hanson, Ryan; Madou, Marc
- Lab on a Chip, Vol. 11, Issue 4, p. 723-726
Assay of blood or other biologic samples for target analytes
patent, June 1997
- Levine, Robert A.; Wardlaw, Stephen C.; Rodriguez, Rodolfo R.
- US Patent Document 5,635,362
Apparatus and method for carrying out analysis of samples
patent, April 1999
- Gordon, John Francis
- US Patent Document 5,892,577
Integrated planar microfluidic bioanalytical systems
patent-application, August 2006
- Hawkins, Aaron R.; Peeni, Bridget; Barber, John
- US Patent Application 11/336646; 20060171654
Multi-parameter assays including analysis discs and methods relating thereto
patent-application, October 2002
- Gordon, John Francis
- US Patent Application 10/121281; 20020151043
Toxin Activity Assays, Devices, Methods And Systems Therefor
patent-application, June 2016
- Koh, Chung-Yan; Schaff, Ulrich Y.; Sommer, Gregory Jon
- US Patent Application 15/056870; 20160178619
Effects of separation length and voltage on isoelectric focusing in a plastic microfluidic device
journal, September 2006
- Das, Champak; Fan, Z. Hugh
- ELECTROPHORESIS, Vol. 27, Issue 18, p. 3619-3626
Class characterization of circulating cancer cells isolated from body fluids and methods of use
patent, November 2005
- Wang, Zheng; Ts'o, Paul O. P.
- US Patent Document 6,960,449
Class characterization of circulating cancer cells isolated from body fluids and methods of use
patent-application, July 2002
- Wang, Zheng-Pin; TS'O, Paul O.P.
- US Patent Document 09/501179; 20020098535
Fully integrated lab-on-a-disc for simultaneous analysis of biochemistry and immunoassay from whole blood
journal, January 2011
- Lee, Beom Seok; Lee, Yang Ui; Kim, Han-Sang
- Lab on a Chip, Vol. 11, Issue 1, p. 70-78
Devices, systems, and methods for conducting sandwich assays using sedimentation
patent, February 2015
- Schaff, Ulrich Y.; Sommer, Gregory J.; Singh, Anup K.
- US Patent Document 8,945,914
Diagnostic tests and kits for Clostridium difficile
patent, January 2003
- Valkirs, Gunars
- US Patent Document 6,503,722
Apparatus and method for measuring white blood cell and platelet concentrations in blood
patent, May 1979
- Wardlaw, Stephen C.
- US Patent Document 4,156,570
DNA purification and isolation using magnetic particles
patent, January 1998
- Hawkins, Trevor
- US Patent Document 5,705,628
System and method for detecting components of a mixture including tooth elements for alignment
patent, November 2016
- Sommer, Gregory J.; Schaff, Ulrich Y.
- US Patent Document 9,500,579
Apparatus for precise transfer and manipulation of fluids by centrifugal and or capillary forces
patent, December 2012
- Pugia, Michael J.; Blankenstein, Gert; Peters, Ralf-Peter
- US Patent Document 8,337,775
Separation Device
patent-application, May 2010
- Froman, Gunnar; Haneskog, Lars; Johansson, Bjorn A.
- US Patent Application12/597925; 20100120596
Loading Disk for Photometric Analyzer of Rotary Cuvette Type
patent, July 1973
- Maddox, Wayne L.; Coleman, Robert L.; Shults III, Wilbur D.
- US Patent Document 3,744,974
Method for separating the cellular components of blood samples
patent, July 1989
- Smith, Ward C.
- US Patent Document 4,844,818
Method for separating cells
patent-application, July 2003
- Woodside, Steven M.
- US Patent Application 10/260560; 2003012471
Multi-parameter assays including analysis discs and methods relating thereto
patent, April 2006
- Gordon, John Francis
- US Patent Document 7,033,747
Microfluidic Devices, Systems, And Methods For Quantifying Particles Using Centrifugal Force
patent-application, December 2015
- Schaff, Ulrich Y.; Sommer, Gregory Jon; Singh, Anup K.
- US Patent Application 14/836665; 20150360225