System and method for detecting components of a mixture including a valving scheme for competition assays

Title: System and method for detecting components of a mixture including a valving scheme for competition assays

Examples are described including measurement systems for conducting competition assays. A first chamber of an assay device may be loaded with a sample containing a target antigen. The target antigen in the sample may be allowed to bind to antibody-coated beads in the first chamber. A control layer separating the first chamber from a second chamber may then be opened to allow a labeling agent loaded in a first portion of the second chamber to bind to any unoccupied sites on the antibodies. A centrifugal force may then be applied to transport the beads through a density media to a detection region for measurement by a detection unit.

Inventors:: Koh, Chung-Yan; Piccini, Matthew E.; Singh, Anup K.

Issue Date:: 2017-09-19

OSTI Identifier:: 1393511

Assignee:: National Technology & Engineering Solutions of Sandia, LLC NNSA

Patent Number(s):: 9,766,230

Application Number:: 15/616,740

Contract Number:: NA0003525

Resource Relation:: Patent File Date: 2017 Jun 07

Research Org:: National Technology & Engineering Solutions of Sandia, LLC., Albuquerque, NM (United States)

Sponsoring Org:: USDOE

Country of Publication:: United States

Language:: English

Subject:: 42 ENGINEERING; 59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION

Full Text
View Full Text

Other works cited in this record:

Multistation, Single Channel Analytical Photometer and Method Of Use
patent, January 1971

Anderson, Norman G.
US Patent Document 3,555,284
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F3555284

Loading disk for photometric analyzer of rotary cuvette type
patent, July 1973

Maddox, Wayne L.; Coleman, Robert L.; Shults, II, Wilbur D.
US Patent Document 3,744,974
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F3744974

Separation of solid and liquid components of mixtures
patent, November 1978

Hunter, William M.
US Patent Document 4,125,375
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F4125375

Apparatus and method for measuring white blood cell and platelet concentrations in blood
patent, May 1979

Wardlaw, Stephen
US Patent Document 4,156,570
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F4156570

Pre-column for preconcentrating substances to be chromatographed as well as device for a liquid chromatography system and coupling member for said device
patent, November 1985

Ruijten, Henri M.; De Bree, Hans; Van Amsterdam, Peter H.
US Patent Document 4,554,071
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F4554071

Heterogeneous binding assay
patent, April 1987

Baker, Terence; Abbott, Stephen; Simpson, John
US Patent Document 4,656,143
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F4656143

Method and apparatus for measuring blood constituent counts
patent, July 1987

Wardlaw, Stephen
US Patent Document 4,683,579
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F4683579

Method for separating the cellular components of blood samples
patent, July 1989

Smith, Ward
US Patent Document 4,844,818
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F4844818

Method of separation employing magnetic particles and second medium
patent, January 1994

Vorpahl, John
US Patent Document 5,279,936
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F5279936

Assay of blood or other biologic samples for target analytes
patent, June 1997

Levine, Robert A.; Wardlaw, Stephen C.; Rodriguez, Rodolfo R.
US Patent Document 5,635,362
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F5635362

DNA purification and isolation using magnetic particles
patent, January 1998

Hawkins, Trevor
US Patent Document 5,705,628
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F5705628

Assay system and method for conducting assays
patent, March 1999

Andrevski, Zygmunt M.; Roach, William Ronald; Southgate, Peter David
US Patent Document 5,882,903
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F5882903

Apparatus and method for carrying out analysis of samples
patent, April 1999

Gordon, John
US Patent Document 5,892,577
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F5892577

Centrifugal hematology disposable
patent, November 2000

Thomas, Bradley S.
US Patent Document 6,153,148
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F6153148

Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system
patent, November 2001

Mian, Alec; Kieffer-Higgins, Stephen G.; Corey, George D.
US Patent Document 6,319,469
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F6319469

Diagnostic tests and kits for Clostridium difficile
patent, January 2003

Valkirs, Gunars E.
US Patent Document 6,503,722
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F6503722

Monolithic microfluidic concentrators and mixers
patent, May 2005

Frechet, Jean M. J.; Svec, Frantisek; Yu, Cong
US Patent Document 6,887,384
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F6887384

Class characterization of circulating cancer cells isolated from body fluids and methods of use
patent, November 2005

Wang, Zheng-Pin; Ts'o, Paul O. P.
US Patent Document 6,960,449
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F6960449

Multi-parameter assays including analysis discs and methods relating thereto
patent, April 2006

Gordon, John Francis
US Patent Document 7,033,747
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F7033747

Optical bio-discs and fluidic circuits for analysis of cells and methods relating thereto
patent, January 2007

Valencia, Ramoncito; Chen, YihFar; Hurt, Susan
US Patent Document 7,157,049
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F7157049

Microfluidic particle-analysis systems
patent, December 2007

Chou, Hou-Pu; Daridon, Antoine; Farrell, Kevin
US Patent Document 7,312,085
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F7312085

Centripetally-motivated microfluidics system for performing in vitro hybridization and amplification of nucleic acids
patent, February 2008

Kellogg, Gregory; Carvalho, Bruce; Sheppard, Norman
US Patent Document 7,332,326
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F7332326

Centrifugal force based microfluidic device, microfluidic system including the same, and method of determining home position of the microfluidic device
patent, July 2010

Lee, Jeong-gun; Lee, Beom-seok; Park, Jong-myeon
US Patent Document 7,758,810
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F7758810

Apparatus for precise transfer and manipulation of fluids by centrifugal and or capillary forces
patent, December 2012

Pugia, Michael J.; Blankenstein, Gert; Peters, Ralf-Peter
US Patent Document 8,337,775
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F8337775

Devices, systems, and methods for conducting sandwich assays using sedimentation
patent, February 2015

Schaff, Ulrich Y.; Sommer, Gregory J.; Singh, Anup K.
US Patent Document 8,945,914
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F8945914

Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation
patent, February 2015

Schaff, Ulrich Y.; Koh, Chung-Yan; Sommer, Gregory J.
US Patent Document 8,962,346
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F8962346

Microfluidic devices, systems, and methods for quantifying particles using centrifugal force
patent, November 2015

Schaff, Ulrich Y.; Sommer, Gregory J.; Singh, Anup K.
US Patent Document 9,186,668
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F9186668

Systems, devices, and methods for agglutination assays using sedimentation
patent, January 2016

Schaff, Ulrich Y.; Sommer, Gregory J.; Singh, Anup K.
US Patent Document 9,244,065
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F9244065

Toxin activity assays, devices, methods and systems therefor
patent, April 2016

Koh, Chung-Yan; Schaff, Ulrich Y.; Sommer, Gregory Jon
US Patent Document 9,304,128
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F9304128

Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation
patent, April 2016

Schaff, Ulrich Y.; Koh, Chung-Yan; Sommer, Gregory J.
US Patent Document 9,304,129
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F9304129

System and method for detecting components of a mixture including tooth elements for alignment
patent, November 2016

Sommer, Gregory; Schaff, Ulrich
US Patent Document 9,500,579
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F9500579

Ricin A-Chain Activity on Stem−Loop and Unstructured DNA Substrates
journal, March 2005

Amukele, Tim K.; Roday, Setu; Schramm, Vern L.
Biochemistry, Vol. 44, Issue 11, p. 4416-4425
DOI: 10.1021/bi0474362

Parallel Nanoliter Microfluidic Analysis System
journal, June 2007

Andersson, Per; Jesson, Gerald; Kylberg, Gunnar
Analytical Chemistry, Vol. 79, Issue 11, p. 4022-4030
DOI: 10.1021/ac061692y

Cell-Free DNA—A Marker to Predict Ischemic Brain Damage in a Rat Stroke Experimental Model
journal, January 2011

Boyko, Matthew; Ohayon, Sharon; Goldsmith, Tomer
Journal of Neurosurgical Anesthesiology, Vol. 23, Issue 3, p. 222-228
DOI: 10.1097/ANA.0b013e31821b536a

Formation of Natural pH Gradients in a Microfluidic Device under Flow Conditions: Model and Experimental Validation
journal, February 2001

Cabrera, Catherine R.; Finlayson, Bruce; Yager, Paul
Analytical Chemistry, Vol. 73, Issue 3, p. 658-666
DOI: 10.1021/ac000495a

Multistage Isoelectric Focusing in a Polymeric Microfluidic Chip
journal, December 2005

Cui, Huanchun; Horiuchi, Keisuke; Dutta, Prashanta
Analytical Chemistry, Vol. 77, Issue 24, p. 7878-7886
DOI: 10.1021/ac050781s

Effects of separation length and voltage on isoelectric focusing in a plastic microfluidic device
journal, September 2006

Das, Champak; Fan, Z. Hugh
ELECTROPHORESIS, Vol. 27, Issue 18, p. 3619-3626
DOI: 10.1002/elps.200600013

Detecting Single Stranded DNA with a Solid State Nanopore
journal, October 2005

Fologea, Daniel; Gershow, Marc; Ledden, Bradley
Nano Letters, Vol. 5, Issue 10, p. 1905-1909
DOI: 10.1021/nl051199m

On-Chip Coupling of Isoelectric Focusing and Free Solution Electrophoresis for Multidimensional Separations
journal, March 2003

Herr, Amy E.; Molho, Joshua I.; Drouvalakis, Katerina A.
Analytical Chemistry, Vol. 75, Issue 5, p. 1180-1187
DOI: 10.1021/ac026239a

Microfabrication of a tapered channel for isoelectric focusing with thermally generated pH gradient
journal, October 2002

Huang, Tiemin; Pawliszyn, Janusz
ELECTROPHORESIS, Vol. 23, Issue 20, p. 3504-3510
DOI: 10.1002/1522-2683(200210)23:20<3504::AID-ELPS3504>3.0.CO;2-X

Rapid isoelectric trapping in a micropreparative-scale multicompartment electrolyzer
journal, June 2007

Lim, Peniel; North, Robert; Vigh, Gyula
ELECTROPHORESIS, Vol. 28, Issue 12, p. 1851-1859
DOI: 10.1002/elps.200600846

Similar records in DOepatents and OSTI.GOV collections:

System and method for detecting components of a mixture including a valving scheme for competition assays

Patent Koh, Chung-Yan ; Piccini, Matthew E. ; Singh, Anup K.

Examples are described including measurement systems for conducting competition assays. A first chamber of an assay device may be loaded with a sample containing a target antigen. The target antigen in the sample may be allowed to bind to antibody-coated beads in the first chamber. A control layer separating the first chamber from a second chamber may then be opened to allow a labeling agent loaded in a first portion of the second chamber to bind to any unoccupied sites on the antibodies. A centrifugal force may then be applied to transport the beads through a density media to amore »« less
Full Text Available July 2017
System and method for detecting components of a mixture including tooth elements for alignment

Patent Sommer, Gregory Jon ; Schaff, Ulrich Y.

Examples are described including assay platforms having tooth elements. An impinging element may sequentially engage tooth elements on the assay platform to sequentially align corresponding detection regions with a detection unit. In this manner, multiple measurements may be made of detection regions on the assay platform without necessarily requiring the starting and stopping of a motor.
Full Text Available November 2016
System and method for detecting cells or components thereof

Patent Porter, Marc D ; Lipert, Robert J ; Doyle, Robert T ; Grubisha, Desiree S ; Rahman, Salma

A system and method for detecting a detectably labeled cell or component thereof in a sample comprising one or more cells or components thereof, at least one cell or component thereof of which is detectably labeled with at least two detectable labels. In one embodiment, the method comprises: (i) introducing the sample into one or more flow cells of a flow cytometer, (ii) irradiating the sample with one or more light sources that are absorbed by the at least two detectable labels, the absorption of which is to be detected, and (iii) detecting simultaneously the absorption of light by themore »« less
Full Text Available January 2009
Method of detecting leakage of reactor core components of liquid metal cooled fast reactors

Patent Holt, Fred E. ; Cash, Robert J. ; Schenter, Robert E.

A method of detecting the failure of a sealed non-fueled core component of a liquid-metal cooled fast reactor having an inert cover gas. A gas mixture is incorporated in the component which includes Xenon-124; under neutron irradiation, Xenon-124 is converted to radioactive Xenon-125. The cover gas is scanned by a radiation detector. The occurrence of 188 Kev gamma radiation and/or other identifying gamma radiation-energy level indicates the presence of Xenon-125 and therefore leakage of a component. Similarly, Xe-126, which transmutes to Xe-127 and Kr-84, which produces Kr-85.sup.m can be used for detection of leakage. Different components are charged with mixturesmore »« less
Full Text Available January 1977
System and method for detecting a faulty object in a system

Patent Gunnels, John A ; Gustavson, Fred Gehrung ; Engle, Robert Daniel

A method (and system) for detecting at least one faulty object in a system including a plurality of objects in communication with each other in an n-dimensional architecture, includes probing a first plane of objects in the n-dimensional architecture and probing at least one other plane of objects in the n-dimensional architecture which would result in identifying a faulty object in the system.
Full Text Available March 2009