Title: System and method for detecting components of a mixture including a valving scheme for competition assays

Abstract

Examples are described including measurement systems for conducting competition assays. A first chamber of an assay device may be loaded with a sample containing a target antigen. The target antigen in the sample may be allowed to bind to antibody-coated beads in the first chamber. A control layer separating the first chamber from a second chamber may then be opened to allow a labeling agent loaded in a first portion of the second chamber to bind to any unoccupied sites on the antibodies. A centrifugal force may then be applied to transport the beads through a density media to a detection region for measurement by a detection unit.

Inventors:

Koh, Chung-Yan; Piccini, Matthew E.; Singh, Anup K.

Issue Date:

2017-09-19

Research Org.:

National Technology & Engineering Solutions of Sandia, LLC., Albuquerque, NM (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1393511

Patent Number(s):

9766230

Application Number:

15/616,740

Assignee:

National Technology & Engineering Solutions of Sandia, LLC

Patent Classifications (CPCs):

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

B01L2300/087 - Multiple sequential chambers
B01L3/502738 - {characterised by integrated valves

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/54306 - {Solid-phase reaction mechanisms}

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

B01L2400/0409 - centrifugal forces
B01L2300/0803 - Disc shape
B01L2200/0647 - Handling flowable solids, e.g. microscopic beads, cells, particles
B01L2200/0684 - Venting, avoiding backpressure, avoid gas bubbles
B01L2400/043 - magnetic forces
B01L2300/0874 - Three dimensional network

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/54366 - {Apparatus specially adapted for solid-phase testing}

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

B01L2400/0683 - mechanically breaking a wall or membrane within a channel or chamber
B01L2400/0677 - phase change valves

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DOE Contract Number:  

NA0003525

Resource Type:

Patent

Resource Relation:

Patent File Date: 2017 Jun 07

Country of Publication:

United States

Language:

English

Subject:

42 ENGINEERING; 59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION