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Title: Variants of glycerol dehydrogenase having D-lactate dehydrogenase activity and uses thereof

Abstract

The present invention provides methods of designing and generating glycerol dehydrogenase (GlyDH) variants that have altered function as compared to a parent polypeptide. The present invention further provides nucleic acids encoding GlyDH polypeptide variants having altered function as compared to the parent polypeptide. Host cells comprising polynucleotides encoding GlyDH variants and methods of producing lactic acids are also provided in various aspects of the invention.

Inventors:
; ;
Issue Date:
Research Org.:
UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATED, Gainesville, FL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1378093
Patent Number(s):
9745556
Application Number:
14/347,688
Assignee:
UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATED
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG36-04GO14019; FG36-08GO88142
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Oct 04
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Wang, Qingzhao, Shanmugam, Keelnatham T., and Ingram, Lonnie O'Neal. Variants of glycerol dehydrogenase having D-lactate dehydrogenase activity and uses thereof. United States: N. p., 2017. Web.
Wang, Qingzhao, Shanmugam, Keelnatham T., & Ingram, Lonnie O'Neal. Variants of glycerol dehydrogenase having D-lactate dehydrogenase activity and uses thereof. United States.
Wang, Qingzhao, Shanmugam, Keelnatham T., and Ingram, Lonnie O'Neal. Tue . "Variants of glycerol dehydrogenase having D-lactate dehydrogenase activity and uses thereof". United States. https://www.osti.gov/servlets/purl/1378093.
@article{osti_1378093,
title = {Variants of glycerol dehydrogenase having D-lactate dehydrogenase activity and uses thereof},
author = {Wang, Qingzhao and Shanmugam, Keelnatham T. and Ingram, Lonnie O'Neal},
abstractNote = {The present invention provides methods of designing and generating glycerol dehydrogenase (GlyDH) variants that have altered function as compared to a parent polypeptide. The present invention further provides nucleic acids encoding GlyDH polypeptide variants having altered function as compared to the parent polypeptide. Host cells comprising polynucleotides encoding GlyDH variants and methods of producing lactic acids are also provided in various aspects of the invention.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {8}
}

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Works referenced in this record:

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