Variants of glycerol dehydrogenase having D-lactate dehydrogenase activity and uses thereof
Abstract
The present invention provides methods of designing and generating glycerol dehydrogenase (GlyDH) variants that have altered function as compared to a parent polypeptide. The present invention further provides nucleic acids encoding GlyDH polypeptide variants having altered function as compared to the parent polypeptide. Host cells comprising polynucleotides encoding GlyDH variants and methods of producing lactic acids are also provided in various aspects of the invention.
- Inventors:
- Issue Date:
- Research Org.:
- UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATED, Gainesville, FL (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1378093
- Patent Number(s):
- 9745556
- Application Number:
- 14/347,688
- Assignee:
- UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATED
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- FG36-04GO14019; FG36-08GO88142
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2012 Oct 04
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Wang, Qingzhao, Shanmugam, Keelnatham T., and Ingram, Lonnie O'Neal. Variants of glycerol dehydrogenase having D-lactate dehydrogenase activity and uses thereof. United States: N. p., 2017.
Web.
Wang, Qingzhao, Shanmugam, Keelnatham T., & Ingram, Lonnie O'Neal. Variants of glycerol dehydrogenase having D-lactate dehydrogenase activity and uses thereof. United States.
Wang, Qingzhao, Shanmugam, Keelnatham T., and Ingram, Lonnie O'Neal. Tue .
"Variants of glycerol dehydrogenase having D-lactate dehydrogenase activity and uses thereof". United States. https://www.osti.gov/servlets/purl/1378093.
@article{osti_1378093,
title = {Variants of glycerol dehydrogenase having D-lactate dehydrogenase activity and uses thereof},
author = {Wang, Qingzhao and Shanmugam, Keelnatham T. and Ingram, Lonnie O'Neal},
abstractNote = {The present invention provides methods of designing and generating glycerol dehydrogenase (GlyDH) variants that have altered function as compared to a parent polypeptide. The present invention further provides nucleic acids encoding GlyDH polypeptide variants having altered function as compared to the parent polypeptide. Host cells comprising polynucleotides encoding GlyDH variants and methods of producing lactic acids are also provided in various aspects of the invention.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {8}
}
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Works referenced in this record:
Production of chemicals from lignocellulose, biomass or sugars
patent, August 2006
- Shanmugam, Keelnatham T.; Ingram, Lonnie O'Neal; Patel, Milind A.
- US Patent Document 7,098,009
Engineered microorganisms for increasing product yield in biotransformations, related methods and systems
patent-application, November 2008
- Peters, Matthew W.; Meinhold, Peter; Buelter, Thomas
- US Patent Application 11/881748; 20080293101
Modified Microorganisms with Inactivated Lactate Dehydrogenase Gene
patent-application, August 2009
- Atkinson, Anthony; Cripps, Roger; Thompson, Ann
- US Patent Application 11/915930; 20090197314
Engineering of Thermotolerant Bacillus Coagulans for Production of D(-)-Lactic Acid
patent-application, May 2012
- Wang, Qingzhao; Shanmugam, Keelnatham T.; Ingram, Lonnie O.
- US Patent Application 13/301836; 20120129231
Evolution of D-lactate dehydrogenase activity from glycerol dehydrogenase and its utility for D-lactate production from lignocellulose
journal, November 2011
- Wang, Q.; Ingram, L. O.; Shanmugam, K. T.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 47, p. 18920-18925
Lactic acid: recent advances in products, processes and technologies — a review
journal, January 2006
- Datta, Rathin; Henry, Michael
- Journal of Chemical Technology & Biotechnology, Vol. 81, Issue 7, p. 1119-1129
Factors affecting the fermentative lactic acid production from renewable resources
journal, February 2000
- Hofvendahl, Karin; Hahn–Hägerdal, Bärbel
- Enzyme and Microbial Technology, Vol. 26, Issue 2-4, p. 87-107
Construction of an Escherichia coli K-12 Mutant for Homoethanologenic Fermentation of Glucose or Xylose without Foreign Genes
journal, January 2007
- Kim, Y.; Ingram, L. O.; Shanmugam, K. T.
- Applied and Environmental Microbiology, Vol. 73, Issue 6, p. 1766-1771
Dihydrolipoamide Dehydrogenase Mutation Alters the NADH Sensitivity of Pyruvate Dehydrogenase Complex of Escherichia coli K-12
journal, March 2008
- Kim, Y.; Ingram, L. O.; Shanmugam, K. T.
- Journal of Bacteriology, Vol. 190, Issue 11, p. 3851-3858
Improved Production of Homo-D-Lactic Acid via Xylose Fermentation by Introduction of Xylose Assimilation Genes and Redirection of the Phosphoketolase Pathway to the Pentose Phosphate Pathway in L-Lactate Dehydrogenase Gene-Deficient Lactobacillus plantarum
journal, October 2009
- Okano, K.; Yoshida, S.; Yamada, R.
- Applied and Environmental Microbiology, Vol. 75, Issue 24, p. 7858-7861
Isolation and Characterization of Acid-Tolerant, Thermophilic Bacteria for Effective Fermentation of Biomass-Derived Sugars to Lactic Acid
journal, May 2006
- Patel, M. A.; Ou, M. S.; Harbrucker, R.
- Applied and Environmental Microbiology, Vol. 72, Issue 5, p. 3228-3235
Lactic acid production by Bacillus coagulans—kinetic studies and optimization of culture medium for batch and continuous fermentations
journal, December 1998
- Payot, T.; Chemaly, Z.; Fick, M.
- Enzyme and Microbial Technology, Vol. 24, Issue 3-4, p. 191-199
Functional Replacement of the Escherichia coliD-(-)-Lactate Dehydrogenase Gene (ldhA) with the L-(+)-Lactate Dehydrogenase Gene (ldhL) from Pediococcus acidilactici
journal, April 2003
- Zhou, S.; Shanmugam, K. T.; Ingram, L. O.
- Applied and Environmental Microbiology, Vol. 69, Issue 4, p. 2237-2244
Escherichia coli Strains Engineered for Homofermentative Production of D-Lactic Acid from Glycerol
journal, May 2010
- Mazumdar, S.; Clomburg, J. M.; Gonzalez, R.
- Applied and Environmental Microbiology, Vol. 76, Issue 13, p. 4327-4336
Dehydrogenation of 1,3-butanediol over Cu-based catalyst
journal, July 2007
- Sato, Satoshi; Takahashi, Ryoji; Fukuda, Hiromitsu
- Journal of Molecular Catalysis A: Chemical, Vol. 272, Issue 1-2, p. 164-168
Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design
journal, August 2005
- Chica, Roberto A.; Doucet, Nicolas; Pelletier, Joelle N.
- Current Opinion in Biotechnology, Vol. 16, Issue 4, p. 378-384
Metabolic Flux Control at the Pyruvate Node in an Anaerobic Escherichia coli Strain with an Active Pyruvate Dehydrogenase
journal, January 2010
- Wang, Q.; Ou, M. S.; Kim, Y.
- Applied and Environmental Microbiology, Vol. 76, Issue 7, p. 2107-2114
Metabolic Engineering of Bacillus subtilis for Ethanol Production: Lactate Dehydrogenase Plays a Key Role in Fermentative Metabolism
journal, June 2007
- Romero, S.; Merino, E.; Bolivar, F.
- Applied and Environmental Microbiology, Vol. 73, Issue 16, p. 5190-5198
One-step production of lactate from cellulose as the sole carbon source without any other organic nutrient by recombinant cellulolytic Bacillus subtilis
journal, July 2011
- Zhang, Xiao-Zhou; Sathitsuksanoh, Noppadon; Zhu, Zhiguang
- Metabolic Engineering, Vol. 13, Issue 4, p. 364-372