Expression of enzymes in yeast for lignocellulose derived oligomer CBP
Abstract
The present invention provides a multi-component enzyme system that hydrolyzes hemicellulose oligomers from hardwood which can be expressed, for example, in yeast such as Saccharomyces cerevisiae. In some embodiments, this invention provides for the engineering of a series of biocatalysts combining the expression and secretion of components of this enzymatic system with robust, rapid xylose utilization, and ethanol fermentation under industrially relevant process conditions for consolidated bioprocessing. In some embodiments, the invention utilizes co-cultures of strains that can achieve significantly improved performance due to the incorporation of additional enzymes in the fermentation system.
- Inventors:
- Issue Date:
- Research Org.:
- Lallemand Hungary Liquidity Management LLC, Budapest, HU (Hungary)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1377880
- Patent Number(s):
- 9745560
- Application Number:
- 14/424,560
- Assignee:
- Lallemand Hungary Liquidity Management LLC
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- FC36-08GO18103
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2013 Mar 15
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS
Citation Formats
McBride, John E., Wiswall, Erin, Shikhare, Indraneel, Xu, Haowen, Thorngren, Naomi, Hau, Heidi H., and Stonehouse, Emily. Expression of enzymes in yeast for lignocellulose derived oligomer CBP. United States: N. p., 2017.
Web.
McBride, John E., Wiswall, Erin, Shikhare, Indraneel, Xu, Haowen, Thorngren, Naomi, Hau, Heidi H., & Stonehouse, Emily. Expression of enzymes in yeast for lignocellulose derived oligomer CBP. United States.
McBride, John E., Wiswall, Erin, Shikhare, Indraneel, Xu, Haowen, Thorngren, Naomi, Hau, Heidi H., and Stonehouse, Emily. Tue .
"Expression of enzymes in yeast for lignocellulose derived oligomer CBP". United States. https://www.osti.gov/servlets/purl/1377880.
@article{osti_1377880,
title = {Expression of enzymes in yeast for lignocellulose derived oligomer CBP},
author = {McBride, John E. and Wiswall, Erin and Shikhare, Indraneel and Xu, Haowen and Thorngren, Naomi and Hau, Heidi H. and Stonehouse, Emily},
abstractNote = {The present invention provides a multi-component enzyme system that hydrolyzes hemicellulose oligomers from hardwood which can be expressed, for example, in yeast such as Saccharomyces cerevisiae. In some embodiments, this invention provides for the engineering of a series of biocatalysts combining the expression and secretion of components of this enzymatic system with robust, rapid xylose utilization, and ethanol fermentation under industrially relevant process conditions for consolidated bioprocessing. In some embodiments, the invention utilizes co-cultures of strains that can achieve significantly improved performance due to the incorporation of additional enzymes in the fermentation system.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {8}
}
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