Nucleic acid constructs containing orthogonal site selective recombinases (OSSRs)
Abstract
The present invention provides for a recombinant nucleic acid comprising a nucleotide sequence comprising a plurality of constructs, wherein each construct independently comprises a nucleotide sequence of interest flanked by a pair of recombinase recognition sequences. Each pair of recombinase recognition sequences is recognized by a distinct recombinase. Optionally, each construct can, independently, further comprise one or more genes encoding a recombinase capable of recognizing the pair of recombinase recognition sequences of the construct. The recombinase can be an orthogonal (non-cross reacting), site-selective recombinase (OSSR).
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1377879
- Patent Number(s):
- 9745587
- Application Number:
- 13/088,288
- Assignee:
- The Regents of the University of California
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC02-05CH11231
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2011 Apr 15
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Gilmore, Joshua M., Anderson, J. Christopher, and Dueber, John E. Nucleic acid constructs containing orthogonal site selective recombinases (OSSRs). United States: N. p., 2017.
Web.
Gilmore, Joshua M., Anderson, J. Christopher, & Dueber, John E. Nucleic acid constructs containing orthogonal site selective recombinases (OSSRs). United States.
Gilmore, Joshua M., Anderson, J. Christopher, and Dueber, John E. Tue .
"Nucleic acid constructs containing orthogonal site selective recombinases (OSSRs)". United States. https://www.osti.gov/servlets/purl/1377879.
@article{osti_1377879,
title = {Nucleic acid constructs containing orthogonal site selective recombinases (OSSRs)},
author = {Gilmore, Joshua M. and Anderson, J. Christopher and Dueber, John E.},
abstractNote = {The present invention provides for a recombinant nucleic acid comprising a nucleotide sequence comprising a plurality of constructs, wherein each construct independently comprises a nucleotide sequence of interest flanked by a pair of recombinase recognition sequences. Each pair of recombinase recognition sequences is recognized by a distinct recombinase. Optionally, each construct can, independently, further comprise one or more genes encoding a recombinase capable of recognizing the pair of recombinase recognition sequences of the construct. The recombinase can be an orthogonal (non-cross reacting), site-selective recombinase (OSSR).},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {8}
}
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