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Title: Reversible, on-demand generation of aqueous two-phase microdroplets

Abstract

The present invention provides methods of on-demand, reversible generation of aqueous two-phase microdroplets core-shell microbeads, microparticle preparations comprising the core-shell microbeads, and drug delivery formulation comprising the microparticle preparations. Because these aqueous microdroplets have volumes comparable to those of cells, they provide an approach to mimicking the dynamic microcompartmentation of biomaterial that naturally occurs within the cytoplasm of cells. Hence, the present methods generate femtoliter aqueous two-phase droplets within a microfluidic oil channel using gated pressure pulses to generate individual, stationary two-phase microdroplets with a well-defined time zero for carrying out controlled and sequential phase transformations over time. Reversible phase transitions between single-phase, two-phase, and core-shell microbead states are obtained via evaporation-induced dehydration and water rehydration.

Inventors:
; ; ;
Issue Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1375237
Patent Number(s):
9730898
Application Number:
13/970,724
Assignee:
UT-BATTELLE, LLC
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 Aug 20
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Collier, Charles Patrick, Retterer, Scott Thomas, Boreyko, Jonathan Barton, and Mruetusatorn, Prachya. Reversible, on-demand generation of aqueous two-phase microdroplets. United States: N. p., 2017. Web.
Collier, Charles Patrick, Retterer, Scott Thomas, Boreyko, Jonathan Barton, & Mruetusatorn, Prachya. Reversible, on-demand generation of aqueous two-phase microdroplets. United States.
Collier, Charles Patrick, Retterer, Scott Thomas, Boreyko, Jonathan Barton, and Mruetusatorn, Prachya. Tue . "Reversible, on-demand generation of aqueous two-phase microdroplets". United States. https://www.osti.gov/servlets/purl/1375237.
@article{osti_1375237,
title = {Reversible, on-demand generation of aqueous two-phase microdroplets},
author = {Collier, Charles Patrick and Retterer, Scott Thomas and Boreyko, Jonathan Barton and Mruetusatorn, Prachya},
abstractNote = {The present invention provides methods of on-demand, reversible generation of aqueous two-phase microdroplets core-shell microbeads, microparticle preparations comprising the core-shell microbeads, and drug delivery formulation comprising the microparticle preparations. Because these aqueous microdroplets have volumes comparable to those of cells, they provide an approach to mimicking the dynamic microcompartmentation of biomaterial that naturally occurs within the cytoplasm of cells. Hence, the present methods generate femtoliter aqueous two-phase droplets within a microfluidic oil channel using gated pressure pulses to generate individual, stationary two-phase microdroplets with a well-defined time zero for carrying out controlled and sequential phase transformations over time. Reversible phase transitions between single-phase, two-phase, and core-shell microbead states are obtained via evaporation-induced dehydration and water rehydration.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {8}
}

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