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Title: In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris

The invention provides orthogonal translation systems for the production of polypeptides comprising unnatural amino acids in methylotrophic yeast such as Pichia pastoris. Methods for producing polypeptides comprising unnatural amino acids in methylotrophic yeast such as Pichia pastoris are also provided.
Inventors:
;
Issue Date:
OSTI Identifier:
1375232
Assignee:
The Scripps Research Institute DOESC
Patent Number(s):
9,732,349
Application Number:
14/165,199
Contract Number:
FG03-00ER46051
Resource Relation:
Patent File Date: 2014 Jan 27
Research Org:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Other works cited in this record:

Glycoprotein synthesis
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In vivo incorporation of unnatural amino acids
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Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs
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Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
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In vivo incorporation of unnatural amino acids
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Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
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Unnatural reactive amino acid genetic code additions
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Site-specific incorporation of redox active amino acids into proteins
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In vivo incorporation of unnatural amino acids
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Expanding the eukaryotic genetic code
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Binding Polypeptides With Diversified And Consensus VH/VL Hypervariable Sequences
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