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Title: In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris

The invention provides orthogonal translation systems for the production of polypeptides comprising unnatural amino acids in methylotrophic yeast such as Pichia pastoris. Methods for producing polypeptides comprising unnatural amino acids in methylotrophic yeast such as Pichia pastoris are also provided.
Inventors:
;
Issue Date:
OSTI Identifier:
1375232
Assignee:
The Scripps Research Institute DOESC
Patent Number(s):
9,732,349
Application Number:
14/165,199
Contract Number:
FG03-00ER46051
Resource Relation:
Patent File Date: 2014 Jan 27
Research Org:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Works referenced in this record:

Genomic Exploration of the Hemiascomycetous Yeasts: 15. Pichia sorbitophila
journal, December 2000

High-Level Expression of Tetanus Toxin Fragment C in Pichia Pastoris Strains Containing Multiple Tandem Integrations of the Gene
journal, May 1991
  • Clare, J. J.; Rayment, F. B.; Ballantine, S. P.
  • Nature Biotechnology, Vol. 9, Issue 5, p. 455-460
  • DOI: 10.1038/nbt0591-455

Yeast Systems for the Commercial Production of Heterologous Proteins
journal, November 1991
  • Buckholz, Richard G.; Gleeson, Martin A. G.
  • Bio/Technology, Vol. 9, Issue 11, p. 1067-1072
  • DOI: 10.1038/nbt1191-1067

Addition of p-Azido-L-phenylalanine to the Genetic Code of Escherichia coli
journal, July 2002
  • Chin, Jason W.; Santoro, Stephen W.; Martin, Andrew B.
  • Journal of the American Chemical Society, Vol. 124, Issue 31, p. 9026-9027
  • DOI: 10.1021/ja027007w

An Expanded Eukaryotic Genetic Code
journal, August 2003
  • Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher
  • Science, Vol. 301, Issue 5635, p. 964-967
  • DOI: 10.1126/science.1084772

Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production
journal, January 2005
  • Daly, Rachel; Hearn, Milton T. W.
  • Journal of Molecular Recognition, Vol. 18, Issue 2, p. 119-138
  • DOI: 10.1002/jmr.687

Adding Amino Acids with Novel Reactivity to the Genetic Code of Saccharomyces Cerevisiae
journal, October 2003
  • Deiters, Alexander; Cropp, T. Ashton; Mukherji, Mridul
  • Journal of the American Chemical Society, Vol. 125, Issue 39, p. 11782-11783
  • DOI: 10.1021/ja0370037

Nucleophilic Catalysis of Oxime Ligation
journal, November 2006
  • Dirksen, Anouk; Hackeng, Tilman M.; Dawson, Philip E.
  • Angewandte Chemie International Edition, Vol. 45, Issue 45, p. 7581-7584
  • DOI: 10.1002/anie.200602877

Overview of Protein Expression in Pichia pastoris
book, December 1995

Control of protein phosphorylation with a genetically encoded photocaged amino acid
journal, October 2007
  • Lemke, Edward A.; Summerer, Daniel; Geierstanger, Bernhard H.
  • Nature Chemical Biology, Vol. 3, Issue 12, p. 769-772
  • DOI: 10.1038/nchembio.2007.44

Optimization of humanized IgGs in glycoengineered Pichia pastoris
journal, January 2006
  • Li, Huijuan; Sethuraman, Natarajan; Stadheim, Terrance A.
  • Nature Biotechnology, Vol. 24, Issue 2, p. 210-215
  • DOI: 10.1038/nbt1178

Detailed Analysis Concerning the Biodistribution and Metabolism of Human Calcitonin-Derived Cell-Penetrating Peptides
journal, August 2008
  • Neundorf, I.; Rennert, R.; Franke, J.
  • Bioconjugate Chemistry, Vol. 19, Issue 8, p. 1596-1603
  • DOI: 10.1021/bc800149f

Optimization of human serum albumin production in methylotrophic yeast Pichia pastoris by repeated fed-batch fermentation
journal, January 2005
  • Ohya, Tomoshi; Ohyama, Masao; Kobayashi, Kaoru
  • Biotechnology and Bioengineering, Vol. 90, Issue 7, p. 876-887
  • DOI: 10.1002/bit.20507

A New Orthogonal Suppressor tRNA/Aminoacyl-tRNA Synthetase Pair for Evolving an Organism with an Expanded Genetic Code
journal, September 2000

Unnatural Amino Acid Incorporation into Virus-Like Particles
journal, April 2008
  • Strable, Erica; Prasuhn, Duane E.; Udit, Andrew K.
  • Bioconjugate Chemistry, Vol. 19, Issue 4, p. 866-875
  • DOI: 10.1021/bc700390r

A genetically encoded fluorescent amino acid
journal, June 2006
  • Summerer, D.; Chen, S.; Wu, N.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 26, p. 9785-9789
  • DOI: 10.1073/pnas.0603965103

New Methods Enabling Efficient Incorporation of Unnatural Amino Acids in Yeast
journal, May 2008
  • Wang, Qian; Wang, Lei
  • Journal of the American Chemical Society, Vol. 130, Issue 19, p. 6066-6067
  • DOI: 10.1021/ja800894n

Addition of the keto functional group to the genetic code of Escherichia coli
journal, December 2002
  • Wang, L.; Zhang, Z.; Brock, A.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 1, p. 56-61
  • DOI: 10.1073/pnas.0234824100

Expanding the Genetic Repertoire of the Methylotrophic Yeast Pichia pastoris
journal, March 2009
  • Young, Travis S.; Ahmad, Insha; Brock, Ansgar
  • Biochemistry, Vol. 48, Issue 12, p. 2643-2653
  • DOI: 10.1021/bi802178k

Developing high cell density fed-batch cultivation strategies for heterologous protein production in Pichia pastoris using the nitrogen source-regulated FLD1 Promoter
journal, May 2005
  • Resina, David; Cos, Oriol; Ferrer, Pau
  • Biotechnology and Bioengineering, Vol. 91, Issue 6, p. 760-767
  • DOI: 10.1002/bit.20545