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Title: Thionin-D4E1 chimeric protein protects plants against bacterial infections

The generation of a chimeric protein containing a first domain encoding either a pro-thionon or thionin, a second domain encoding D4E1 or pro-D4E1, and a third domain encoding a peptide linker located between the first domain and second domain is described. Either the first domain or the second domain is located at the amino terminal of the chimeric protein and the other domain (second domain or first domain, respectively) is located at the carboxyl terminal. The chimeric protein has antibacterial activity. Genetically altered plants and their progeny expressing a polynucleotide encoding the chimeric protein resist diseases caused by bacteria.
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OSTI Identifier:
The United States of America, as represented by the Secretary of Agriculture LANL
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Resource Relation:
Patent File Date: 2015 Apr 21
Research Org:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
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Country of Publication:
United States

Works referenced in this record:

Expression of a magainin-type antimicrobial peptide gene (MSI-99) in tomato enhances resistance to bacterial speck disease
journal, January 2004

Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoter
journal, July 2013
  • Attílio, Lísia Borges; Mourão Filho, Francisco de Assis Alves; Harakava, Ricardo
  • Pesquisa Agropecuária Brasileira, Vol. 48, Issue 7, p. 741-747
  • DOI: 10.1590/S0100-204X2013000700006

In Vitro Microbicidal Activities of Cecropin Peptides D2A21 and D4E1 and Gel Formulations Containing 0.1 to 2% D2A21 against Chlamydia trachomatis
journal, January 2002
  • Ballweber, L. M.; Jaynes, J. E.; Stamm, W. E.
  • Antimicrobial Agents and Chemotherapy, Vol. 46, Issue 1, p. 34-41
  • DOI: 10.1128/AAC.46.1.34-41.2002

Expression of the α-thionin gene from barley in tobacco confers enhanced resistance to bacterial pathogens
journal, March 1993

Transgenic expression of a gene encoding a synthetic antimicrobial peptide results in inhibition of fungal growth in vitro and in planta
journal, May 2000

Cecropin A—Derived Peptides Are Potent Inhibitors of Fungal Plant Pathogens
journal, March 1998
  • Cavallarin, Laura; Andreu, David; San Segundo, Blanca
  • Molecular Plant-Microbe Interactions, Vol. 11, Issue 3, p. 218-227
  • DOI: 10.1094/MPMI.1998.11.3.218

Enhanced resistance to the rice blast fungus Magnaporthe grisea conferred by expression of a cecropin A gene in transgenic rice
journal, October 2005

An engineered innate immune defense protects grapevines from Pierce disease
journal, February 2012
  • Dandekar, A. M.; Gouran, H.; Ibanez, A. M.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 10, p. 3721-3725
  • DOI: 10.1073/pnas.1116027109

Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1
journal, June 1998
  • De Lucca, A. J.; Bland, J. M.; Grimm, C.
  • Canadian Journal of Microbiology, Vol. 44, Issue 6, p. 514-520
  • DOI: 10.1139/w98-032

Overexpression of an Endogenous Thionin Enhances Resistance of Arabidopsis against Fusarium oxysporum
journal, April 1997
  • Epple, P.; Apel, K.; Bohlmann, H.
  • The Plant Cell Online, Vol. 9, Issue 4, p. 509-520
  • DOI: 10.1105/tpc.9.4.509

Acquired resistance to the rice blast in transgenic rice accumulating the antimicrobial peptide thanatin
journal, September 2009
  • Imamura, Tomohiro; Yasuda, Michiko; Kusano, Hiroaki
  • Transgenic Research, Vol. 19, Issue 3, p. 415-424
  • DOI: 10.1007/s11248-009-9320-x

Enhanced Resistance to Seed-Transmitted Bacterial Diseases in Transgenic Rice Plants Overproducing an Oat Cell-Wall-Bound Thionin
journal, June 2002
  • Iwai, Takayoshi; Kaku, H.; Honkura, R.
  • Molecular Plant-Microbe Interactions, Vol. 15, Issue 6, p. 515-521
  • DOI: 10.1094/MPMI.2002.15.6.515

Expression of a Cecropin B lytic peptide analog in transgenic tobacco confers enhanced resistance to bacterial wilt caused by Pseudomonas solanacearum
journal, January 1993

Enhanced resistance to bacterial and fungal pathogens by overexpression of a human cathelicidin antimicrobial peptide (hCAP18/LL-37) in Chinese cabbage
journal, October 2011
  • Jung, Yu-Jin; Lee, Soon-Youl; Moon, Yong-Sun
  • Plant Biotechnology Reports, Vol. 6, Issue 1, p. 39-46
  • DOI: 10.1007/s11816-011-0193-0

Involvement of the Pepper Antimicrobial Protein CaAMP1 Gene in Broad Spectrum Disease Resistance
journal, August 2008
  • Lee, S. C.; Hwang, I. S.; Choi, H. W.
  • Plant Physiology , Vol. 148, Issue 2, p. 1004-1020
  • DOI: 10.1104/pp.108.123836

Identification of Novel Hexapeptides Bioactive against Phytopathogenic Fungi through Screening of a Synthetic Peptide Combinatorial Library
journal, May 2002
  • Lopez-Garcia, B.; Perez-Paya, E.; Marcos, J. F.
  • Applied and Environmental Microbiology, Vol. 68, Issue 5, p. 2453-2460
  • DOI: 10.1128/AEM.68.5.2453-2460.2002

Identification and Characterization of a Hexapeptide with Activity Against Phytopathogenic Fungi That Cause Postharvest Decay in Fruits
journal, August 2000
  • López-García, Belén; González-Candelas, Luis; Pérez-Payá, Enrique
  • Molecular Plant-Microbe Interactions, Vol. 13, Issue 8, p. 837-846
  • DOI: 10.1094/MPMI.2000.13.8.837

Bacterial disease resistance of transgenic hybrid poplar expressing the synthetic antimicrobial peptide D4E1
journal, April 2003

Inhibition of bacterial and fungal plant pathogens by thionins of types I and II
journal, January 1993

De novo designed cyclic cationic peptides as inhibitors of plant pathogenic bacteria
journal, November 2006

Improvement of cyclic decapeptides against plant pathogenic bacteria using a combinatorial chemistry approach
journal, November 2006

Antimicrobial peptides and plant disease control
journal, May 2007

Transgenic sweet potato expressing thionin from barley gives resistance to black rot disease caused by Ceratocystis fimbriata in leaves and storage roots
journal, January 2012
  • Muramoto, Nobuhiko; Tanaka, Tomoko; Shimamura, Takashi
  • Plant Cell Reports, Vol. 31, Issue 6, p. 987-997
  • DOI: 10.1007/s00299-011-1217-5

Deciphering a mechanism of membrane permeabilization by α-hordothionin peptide
journal, June 2011

Transgenic Potatoes Expressing a Novel Cationic Peptide are Resistant to Late Blight and Pink Rot
journal, April 2004

Broad-Spectrum Antimicrobial Activity in vitro of the Synthetic Peptide D4E1
journal, June 2001
  • Rajasekaran, Kanniah; Stromberg, Kurt D.; Cary, Jeffrey W.
  • Journal of Agricultural and Food Chemistry, Vol. 49, Issue 6, p. 2799-2803
  • DOI: 10.1021/jf010154d

Disease resistance conferred by the expression of a gene encoding a synthetic peptide in transgenic cotton (Gossypium hirsutum L.) plants
journal, November 2005

First evidence for improved resistance to fire blight in transgenic pear expressing the attacin E gene from Hyalophora cecropia
journal, November 1999