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Title: Glycoside hydrolases having multiple hydrolase activities

Abstract

Glycoside hydrolases having at least two different hydrolytic activities are provided. In one embodiment, an isolated recombinant hydrolase having at least two activities selected from a group including asparagine derivatives, glutamine derivatives, and histidine derivatives is provided. Further, a method of generating free sugars from a mixture comprising asparagine derivatives, glutamine derivatives, and histidine derivatives is provided.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1374579
Patent Number(s):
9725749
Application Number:
14/114,990
Assignee:
The Regents of the University of California
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 May 01
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Chen, Zhiwei, Friedland, Gregory D., Chhabra, Swapnil R., Chivian, Dylan C., and Simmons, Blake A. Glycoside hydrolases having multiple hydrolase activities. United States: N. p., 2017. Web.
Chen, Zhiwei, Friedland, Gregory D., Chhabra, Swapnil R., Chivian, Dylan C., & Simmons, Blake A. Glycoside hydrolases having multiple hydrolase activities. United States.
Chen, Zhiwei, Friedland, Gregory D., Chhabra, Swapnil R., Chivian, Dylan C., and Simmons, Blake A. Tue . "Glycoside hydrolases having multiple hydrolase activities". United States. https://www.osti.gov/servlets/purl/1374579.
@article{osti_1374579,
title = {Glycoside hydrolases having multiple hydrolase activities},
author = {Chen, Zhiwei and Friedland, Gregory D. and Chhabra, Swapnil R. and Chivian, Dylan C. and Simmons, Blake A},
abstractNote = {Glycoside hydrolases having at least two different hydrolytic activities are provided. In one embodiment, an isolated recombinant hydrolase having at least two activities selected from a group including asparagine derivatives, glutamine derivatives, and histidine derivatives is provided. Further, a method of generating free sugars from a mixture comprising asparagine derivatives, glutamine derivatives, and histidine derivatives is provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {8}
}

Works referenced in this record:

Regulation of Endo-Acting Glycosyl Hydrolases in the Hyperthermophilic Bacterium Thermotoga maritima Grown on Glucan- and Mannan-Based Polysaccharides
journal, February 2002


Site-directed mutagenesis and CBM engineering of Cel5A (Thermotoga maritima)
journal, October 2008


Biochemical characterization and crystal structure of endoglucanase Cel5A from the hyperthermophilic Thermotoga maritima
journal, December 2010