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Title: Glycoside hydrolases having multiple hydrolase activities

Glycoside hydrolases having at least two different hydrolytic activities are provided. In one embodiment, an isolated recombinant hydrolase having at least two activities selected from a group including asparagine derivatives, glutamine derivatives, and histidine derivatives is provided. Further, a method of generating free sugars from a mixture comprising asparagine derivatives, glutamine derivatives, and histidine derivatives is provided.
Inventors:
; ; ; ;
Issue Date:
OSTI Identifier:
1374579
Assignee:
The Regents of the University of California LBNL
Patent Number(s):
9,725,749
Application Number:
14/114,990
Contract Number:
AC02-05CH11231
Resource Relation:
Patent File Date: 2012 May 01
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Other works cited in this record:

Carboxymethyl cellulase fromthermotoga maritima
patent, October 1999

Methods for generating cellulases
patent, September 2008

Glucanases, Nucleic Acids Encoding Them And Methods For Making And Using Them
patent-application, September 2008

Regulation of Endo-Acting Glycosyl Hydrolases in the Hyperthermophilic Bacterium Thermotoga maritima Grown on Glucan- and Mannan-Based Polysaccharides
journal, February 2002
  • Chhabra, S. R.; Shockley, K. R.; Ward, D. E.
  • Applied and Environmental Microbiology, Vol. 68, Issue 2, p. 545-554
  • DOI: 10.1128/AEM.68.2.545-554.2002

Site-directed mutagenesis and CBM engineering of Cel5A (Thermotoga maritima)
journal, October 2008

Biochemical characterization and crystal structure of endoglucanase Cel5A from the hyperthermophilic Thermotoga maritima
journal, December 2010
  • Pereira, Jose H.; Chen, Zhiwei; McAndrew, Ryan P.
  • Journal of Structural Biology, Vol. 172, Issue 3, p. 372-379
  • DOI: 10.1016/j.jsb.2010.06.018

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