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Title: System and method for detecting components of a mixture including a valving scheme for competition assays

Abstract

Examples are described including measurement systems for conducting competition assays. A first chamber of an assay device may be loaded with a sample containing a target antigen. The target antigen in the sample may be allowed to bind to antibody-coated beads in the first chamber. A control layer separating the first chamber from a second chamber may then be opened to allow a labeling agent loaded in a first portion of the second chamber to bind to any unoccupied sites on the antibodies. A centrifugal force may then be applied to transport the beads through a density media to a detection region for measurement by a detection unit.

Inventors:
; ;
Issue Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1369070
Patent Number(s):
9702871
Application Number:
14/546,876
Assignee:
National Technology & Engineering Solutions of Sandia, LLC
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Resource Relation:
Patent File Date: 2014 Nov 18
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Koh, Chung-Yan, Piccini, Matthew E., and Singh, Anup K. System and method for detecting components of a mixture including a valving scheme for competition assays. United States: N. p., 2017. Web.
Koh, Chung-Yan, Piccini, Matthew E., & Singh, Anup K. System and method for detecting components of a mixture including a valving scheme for competition assays. United States.
Koh, Chung-Yan, Piccini, Matthew E., and Singh, Anup K. Tue . "System and method for detecting components of a mixture including a valving scheme for competition assays". United States. https://www.osti.gov/servlets/purl/1369070.
@article{osti_1369070,
title = {System and method for detecting components of a mixture including a valving scheme for competition assays},
author = {Koh, Chung-Yan and Piccini, Matthew E. and Singh, Anup K.},
abstractNote = {Examples are described including measurement systems for conducting competition assays. A first chamber of an assay device may be loaded with a sample containing a target antigen. The target antigen in the sample may be allowed to bind to antibody-coated beads in the first chamber. A control layer separating the first chamber from a second chamber may then be opened to allow a labeling agent loaded in a first portion of the second chamber to bind to any unoccupied sites on the antibodies. A centrifugal force may then be applied to transport the beads through a density media to a detection region for measurement by a detection unit.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {7}
}

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