Global to push GA events into
skip to main content

Title: System and method for detecting components of a mixture including a valving scheme for competition assays

Examples are described including measurement systems for conducting competition assays. A first chamber of an assay device may be loaded with a sample containing a target antigen. The target antigen in the sample may be allowed to bind to antibody-coated beads in the first chamber. A control layer separating the first chamber from a second chamber may then be opened to allow a labeling agent loaded in a first portion of the second chamber to bind to any unoccupied sites on the antibodies. A centrifugal force may then be applied to transport the beads through a density media to a detection region for measurement by a detection unit.
Inventors:
; ;
Issue Date:
OSTI Identifier:
1369070
Assignee:
National Technology & Engineering Solutions of Sandia, LLC SNL-A
Patent Number(s):
9,702,871
Application Number:
14/546,876
Contract Number:
AC04-94AL85000
Resource Relation:
Patent File Date: 2014 Nov 18
Research Org:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Other works cited in this record:

Multistation, Single Channel Analytical Photometer and Method Of Use
patent, January 1971

Loading disk for photometric analyzer of rotary cuvette type
patent, July 1973

Separation of solid and liquid components of mixtures
patent, November 1978

Apparatus and method for measuring white blood cell and platelet concentrations in blood
patent, May 1979

Pre-column for preconcentrating substances to be chromatographed as well as device for a liquid chromatography system and coupling member for said device
patent, November 1985

Heterogeneous binding assay
patent, April 1987

Method and apparatus for measuring blood constituent counts
patent, July 1987

Method for separating the cellular components of blood samples
patent, July 1989

Method of separation employing magnetic particles and second medium
patent, January 1994

Assay of blood or other biologic samples for target analytes
patent, June 1997

DNA purification and isolation using magnetic particles
patent, January 1998

Assay system and method for conducting assays
patent, March 1999

Centrifugal hematology disposable
patent, November 2000

Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system
patent, November 2001

Diagnostic tests and kits for Clostridium difficile
patent, January 2003

Monolithic microfluidic concentrators and mixers
patent, May 2005

Class characterization of circulating cancer cells isolated from body fluids and methods of use
patent, November 2005

Multi-parameter assays including analysis discs and methods relating thereto
patent, April 2006

Optical bio-discs and fluidic circuits for analysis of cells and methods relating thereto
patent, January 2007

Microfluidic particle-analysis systems
patent, December 2007

Centripetally-motivated microfluidics system for performing in vitro hybridization and amplification of nucleic acids
patent, February 2008

Centrifugal force based microfluidic device, microfluidic system including the same, and method of determining home position of the microfluidic device
patent, July 2010

Apparatus for precise transfer and manipulation of fluids by centrifugal and or capillary forces
patent, December 2012

Devices, systems, and methods for conducting sandwich assays using sedimentation
patent, February 2015

Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation
patent, February 2015

Microfluidic devices, systems, and methods for quantifying particles using centrifugal force
patent, November 2015

Systems, devices, and methods for agglutination assays using sedimentation
patent, January 2016

Toxin activity assays, devices, methods and systems therefor
patent, April 2016

Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation
patent, April 2016

Devices and Method For Using Centripetal Acceleration To Drive Fluid Movement In A Microfluidics System With On-Board Informatics
patent-application, December 2001

Class characterization of circulating cancer cells isolated from body fluids and methods of use
patent-application, July 2002

Microfluidics devices and methods for performing cell based assays
patent-application, August 2002

Reagents for labeling biomolecules having aldehyde or ketone Moieties
patent-application, September 2002

Multi-parameter assays including analysis discs and methods relating thereto
patent-application, October 2002

Multichannel control in microfluidics
patent-application, October 2002

Analytical methods and compositions
patent-application, November 2002

Plastic microfluidics enabling two-dimensional protein separations in proteome analysis
patent-application, November 2002

Microfluidic devices and methods
patent-application, January 2003

Method for separating cells
patent-application, July 2003

Diffusion-based system and method for detecting and monitoring activity of biologic and chemical species
patent-application, October 2003

Microfluidic particle-analysis systems
patent-application, April 2004

Protecting agent
patent-application, August 2005

Centrifuge with removable core for scalable centrifugation
patent-application, September 2005

Microparticle-based methods and systems and applications thereof
patent-application, December 2005

Integrated planar microfluidic bioanalytical systems
patent-application, August 2006

Method for Separating Cells
patent-application, May 2008

Method Of Detecting Analyte-Molecule Interactions
patent-application, June 2008

Method and Apparatus For Precise Transfer and Manipulation Of Fluids By Centrifugal and Or Capillary Forces
patent-application, January 2009

Method For Isolating Nucleic Acids Comprising The Use Of Ethylene Glycol Multimers
patent-application, March 2009

Centrifugal Separation System
patent-application, August 2009

Method for detecting analytes in a sample
patent-application, December 2009

Density-based cell detection system
patent, March 2010

Separation Device
patent-application, May 2010

Compact disk based platform for separating and detecting immunomagnetic bead labeled cells
patent-application, June 2010

Centrifugation Apparatus For Containers Of Biological Material
patent-application, February 2011

Diagnostic Analyzers With Pretreatment Carousels and Related Methods
patent-application, September 2014

Microfluidic Devices, Systems, And Methods For Quantifying Particles Using Centrifugal Force
patent-application, December 2015

Systems, Devices, and Methods For Agglutination Assays Using Sedimentation
patent-application, March 2016

Toxin Activity Assays, Devices, Methods And Systems Therefor
patent-application, June 2016

Polyethyleneimine functionalized iron oxide nanoparticles as agents for DNA delivery and transfection
journal, January 2007
  • McBain, S. C.; Yiu, H. H. P.; El Haj, A.
  • Journal of Materials Chemistry, Vol. 17, Issue 24, p. 2561-2565
  • DOI: 10.1039/B617402G

Micro freef-low IEF enhanced by active cooling and functionalized gels
journal, December 2006
  • Albrecht, Jacob W.; Jensen, Klavs F.
  • ELECTROPHORESIS, Vol. 27, Issue 24, p. 4960-4969
  • DOI: 10.1002/elps.200600436

Ricin A-Chain Activity on Stem−Loop and Unstructured DNA Substrates
journal, March 2005
  • Amukele, Tim K.; Roday, Setu; Schramm, Vern L.
  • Biochemistry, Vol. 44, Issue 11, p. 4416-4425
  • DOI: 10.1021/bi0474362

Parallel Nanoliter Microfluidic Analysis System
journal, June 2007
  • Andersson, Per; Jesson, Gerald; Kylberg, Gunnar
  • Analytical Chemistry, Vol. 79, Issue 11, p. 4022-4030
  • DOI: 10.1021/ac061692y

Cell-Free DNA—A Marker to Predict Ischemic Brain Damage in a Rat Stroke Experimental Model
journal, January 2011
  • Boyko, Matthew; Ohayon, Sharon; Goldsmith, Tomer
  • Journal of Neurosurgical Anesthesiology, Vol. 23, Issue 3, p. 222-228
  • DOI: 10.1097/ANA.0b013e31821b536a

Plasma DNA concentration as a predictor of mortality and sepsis in critically ill patients
journal, April 2006
  • Rhodes, Andrew; Wort, Stephen J.; Thomas, Helen
  • Critical Care, Vol. 10, Issue 2, Article No. R60
  • DOI: 10.1186/cc4894

A B Cell-Based Sensor for Rapid Identification of Pathogens
journal, July 2003
  • Rider, Todd H.; Petrovick, Martha S.; Nargi, Frances E.
  • Science, Vol. 301, Issue 5630, p. 213-215
  • DOI: 10.1126/science.1084920

Whole Blood Immunoassay Based on Centrifugal Bead Sedimentation
journal, March 2011

Characterization of EBV-genome negative “null” and “T” cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma
journal, May 1977
  • Schneider, Ulrich; Schwenk, Hans-Ulrich; Bornkamm, Georg
  • International Journal of Cancer, Vol. 19, Issue 5, p. 621-626
  • DOI: 10.1002/ijc.2910190505

Formation of Natural pH Gradients in a Microfluidic Device under Flow Conditions:  Model and Experimental Validation
journal, February 2001
  • Cabrera, Catherine R.; Finlayson, Bruce; Yager, Paul
  • Analytical Chemistry, Vol. 73, Issue 3, p. 658-666
  • DOI: 10.1021/ac000495a

Multistage Isoelectric Focusing in a Polymeric Microfluidic Chip
journal, December 2005
  • Cui, Huanchun; Horiuchi, Keisuke; Dutta, Prashanta
  • Analytical Chemistry, Vol. 77, Issue 24, p. 7878-7886
  • DOI: 10.1021/ac050781s

Effects of separation length and voltage on isoelectric focusing in a plastic microfluidic device
journal, September 2006

Integrated Preconcentration SDS−PAGE of Proteins in Microchips Using Photopatterned Cross-Linked Polyacrylamide Gels
journal, July 2006
  • Hatch, Anson V.; Herr, Amy E.; Throckmorton, Daniel J.
  • Analytical Chemistry, Vol. 78, Issue 14, p. 4976-4984
  • DOI: 10.1021/ac0600454

Microfluidic immunoassays as rapid saliva-based clinical diagnostics
journal, March 2007
  • Herr, A. E.; Hatch, A. V.; Throckmorton, D. J.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 13, p. 5268-5273
  • DOI: 10.1073/pnas.0607254104

On-Chip Coupling of Isoelectric Focusing and Free Solution Electrophoresis for Multidimensional Separations
journal, March 2003
  • Herr, Amy E.; Molho, Joshua I.; Drouvalakis, Katerina A.
  • Analytical Chemistry, Vol. 75, Issue 5, p. 1180-1187
  • DOI: 10.1021/ac026239a

Rapid isoelectric trapping in a micropreparative-scale multicompartment electrolyzer
journal, June 2007
  • Lim, Peniel; North, Robert; Vigh, Gyula
  • ELECTROPHORESIS, Vol. 28, Issue 12, p. 1851-1859
  • DOI: 10.1002/elps.200600846

Integration of nanoporous membranes for sample filtration/preconcentration in microchip electrophoresis
journal, December 2006
  • Long, Zhicheng; Liu, Dayu; Ye, Nannan
  • ELECTROPHORESIS, Vol. 27, Issue 24, p. 4927-4934
  • DOI: 10.1002/elps.200600252

On-Chip Isoelectric Focusing Using Photopolymerized Immobilized pH Gradients
journal, May 2008
  • Sommer, Greg J.; Singh, Anup K.; Hatch, Anson V.
  • Analytical Chemistry, Vol. 80, Issue 9, p. 3327-3333
  • DOI: 10.1021/ac702523g

Miniaturized capillary isoelectric focusing in plastic microfluidic devices
journal, October 2002

Parallel isoelectric focusing chip
journal, September 2004
  • Zilberstein, Gleb; Korol, Leonid; Bukshpan, Shmuel
  • PROTEOMICS, Vol. 4, Issue 9, p. 2533-2540
  • DOI: 10.1002/pmic.200300794

Parallel isoelectric focusing II
journal, November 2004
  • Zilberstein, Gleb V.; Baskin, Emmanuil M.; Bukshpan, Shmuel
  • ELECTROPHORESIS, Vol. 25, Issue 21-22, p. 3643-3651
  • DOI: 10.1002/elps.200406117

Parallel processing in the isoelectric focusing chip
journal, November 2003
  • Zilberstein, Gleb V.; Baskin, Emmanuil M.; Bukshpan, Shmuel
  • ELECTROPHORESIS, Vol. 24, Issue 21, p. 3735-3744
  • DOI: 10.1002/elps.200305663

Similar records in DOepatents and OSTI.GOV collections: