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Title: System and method for detecting components of a mixture including a valving scheme for competition assays

Examples are described including measurement systems for conducting competition assays. A first chamber of an assay device may be loaded with a sample containing a target antigen. The target antigen in the sample may be allowed to bind to antibody-coated beads in the first chamber. A control layer separating the first chamber from a second chamber may then be opened to allow a labeling agent loaded in a first portion of the second chamber to bind to any unoccupied sites on the antibodies. A centrifugal force may then be applied to transport the beads through a density media to a detection region for measurement by a detection unit.
Inventors:
; ;
Issue Date:
OSTI Identifier:
1369070
Assignee:
National Technology & Engineering Solutions of Sandia, LLC SNL-A
Patent Number(s):
9,702,871
Application Number:
14/546,876
Contract Number:
AC04-94AL85000
Resource Relation:
Patent File Date: 2014 Nov 18
Research Org:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Works referenced in this record:

Protecting agent
patent-application, August 2005

Polyethyleneimine functionalized iron oxide nanoparticles as agents for DNA delivery and transfection
journal, January 2007
  • McBain, S. C.; Yiu, H. H. P.; El Haj, A.
  • Journal of Materials Chemistry, Vol. 17, Issue 24, p. 2561-2565
  • DOI: 10.1039/B617402G

Micro freef-low IEF enhanced by active cooling and functionalized gels
journal, December 2006
  • Albrecht, Jacob W.; Jensen, Klavs F.
  • ELECTROPHORESIS, Vol. 27, Issue 24, p. 4960-4969
  • DOI: 10.1002/elps.200600436

Ricin A-Chain Activity on Stem−Loop and Unstructured DNA Substrates
journal, March 2005
  • Amukele, Tim K.; Roday, Setu; Schramm, Vern L.
  • Biochemistry, Vol. 44, Issue 11, p. 4416-4425
  • DOI: 10.1021/bi0474362

Parallel Nanoliter Microfluidic Analysis System
journal, June 2007
  • Andersson, Per; Jesson, Gerald; Kylberg, Gunnar
  • Analytical Chemistry, Vol. 79, Issue 11, p. 4022-4030
  • DOI: 10.1021/ac061692y

Cell-Free DNA—A Marker to Predict Ischemic Brain Damage in a Rat Stroke Experimental Model
journal, January 2011
  • Boyko, Matthew; Ohayon, Sharon; Goldsmith, Tomer
  • Journal of Neurosurgical Anesthesiology, Vol. 23, Issue 3, p. 222-228
  • DOI: 10.1097/ANA.0b013e31821b536a

Plasma DNA concentration as a predictor of mortality and sepsis in critically ill patients
journal, April 2006
  • Rhodes, Andrew; Wort, Stephen J.; Thomas, Helen
  • Critical Care, Vol. 10, Issue 2, Article No. R60
  • DOI: 10.1186/cc4894

A B Cell-Based Sensor for Rapid Identification of Pathogens
journal, July 2003
  • Rider, Todd H.; Petrovick, Martha S.; Nargi, Frances E.
  • Science, Vol. 301, Issue 5630, p. 213-215
  • DOI: 10.1126/science.1084920

Whole Blood Immunoassay Based on Centrifugal Bead Sedimentation
journal, March 2011

Characterization of EBV-genome negative “null” and “T” cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma
journal, May 1977
  • Schneider, Ulrich; Schwenk, Hans-Ulrich; Bornkamm, Georg
  • International Journal of Cancer, Vol. 19, Issue 5, p. 621-626
  • DOI: 10.1002/ijc.2910190505

Formation of Natural pH Gradients in a Microfluidic Device under Flow Conditions:  Model and Experimental Validation
journal, February 2001
  • Cabrera, Catherine R.; Finlayson, Bruce; Yager, Paul
  • Analytical Chemistry, Vol. 73, Issue 3, p. 658-666
  • DOI: 10.1021/ac000495a

Multistage Isoelectric Focusing in a Polymeric Microfluidic Chip
journal, December 2005
  • Cui, Huanchun; Horiuchi, Keisuke; Dutta, Prashanta
  • Analytical Chemistry, Vol. 77, Issue 24, p. 7878-7886
  • DOI: 10.1021/ac050781s

Effects of separation length and voltage on isoelectric focusing in a plastic microfluidic device
journal, September 2006

Integrated Preconcentration SDS−PAGE of Proteins in Microchips Using Photopatterned Cross-Linked Polyacrylamide Gels
journal, July 2006
  • Hatch, Anson V.; Herr, Amy E.; Throckmorton, Daniel J.
  • Analytical Chemistry, Vol. 78, Issue 14, p. 4976-4984
  • DOI: 10.1021/ac0600454

Microfluidic immunoassays as rapid saliva-based clinical diagnostics
journal, March 2007
  • Herr, A. E.; Hatch, A. V.; Throckmorton, D. J.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 13, p. 5268-5273
  • DOI: 10.1073/pnas.0607254104

On-Chip Coupling of Isoelectric Focusing and Free Solution Electrophoresis for Multidimensional Separations
journal, March 2003
  • Herr, Amy E.; Molho, Joshua I.; Drouvalakis, Katerina A.
  • Analytical Chemistry, Vol. 75, Issue 5, p. 1180-1187
  • DOI: 10.1021/ac026239a

Rapid isoelectric trapping in a micropreparative-scale multicompartment electrolyzer
journal, June 2007
  • Lim, Peniel; North, Robert; Vigh, Gyula
  • ELECTROPHORESIS, Vol. 28, Issue 12, p. 1851-1859
  • DOI: 10.1002/elps.200600846

Integration of nanoporous membranes for sample filtration/preconcentration in microchip electrophoresis
journal, December 2006
  • Long, Zhicheng; Liu, Dayu; Ye, Nannan
  • ELECTROPHORESIS, Vol. 27, Issue 24, p. 4927-4934
  • DOI: 10.1002/elps.200600252

On-Chip Isoelectric Focusing Using Photopolymerized Immobilized pH Gradients
journal, May 2008
  • Sommer, Greg J.; Singh, Anup K.; Hatch, Anson V.
  • Analytical Chemistry, Vol. 80, Issue 9, p. 3327-3333
  • DOI: 10.1021/ac702523g

Miniaturized capillary isoelectric focusing in plastic microfluidic devices
journal, October 2002

Parallel isoelectric focusing chip
journal, September 2004
  • Zilberstein, Gleb; Korol, Leonid; Bukshpan, Shmuel
  • PROTEOMICS, Vol. 4, Issue 9, p. 2533-2540
  • DOI: 10.1002/pmic.200300794

Parallel isoelectric focusing II
journal, November 2004
  • Zilberstein, Gleb V.; Baskin, Emmanuil M.; Bukshpan, Shmuel
  • ELECTROPHORESIS, Vol. 25, Issue 21-22, p. 3643-3651
  • DOI: 10.1002/elps.200406117

Parallel processing in the isoelectric focusing chip
journal, November 2003
  • Zilberstein, Gleb V.; Baskin, Emmanuil M.; Bukshpan, Shmuel
  • ELECTROPHORESIS, Vol. 24, Issue 21, p. 3735-3744
  • DOI: 10.1002/elps.200305663