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Title: System and method for measuring fluorescence of a sample

Abstract

The present disclosure provides a system and a method for measuring fluorescence of a sample. The sample may be a polymerase-chain-reaction (PCR) array, a loop-mediated-isothermal amplification array, etc. LEDs are used to excite the sample, and a photodiode is used to collect the sample's fluorescence. An electronic offset signal is used to reduce the effects of background fluorescence and the noises from the measurement system. An integrator integrates the difference between the output of the photodiode and the electronic offset signal over a given period of time. The resulting integral is then converted into digital domain for further processing and storage.

Inventors:
Issue Date:
Research Org.:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1366507
Patent Number(s):
9689799
Application Number:
14/602,076
Assignee:
Lawrence Livermore National Security, LLC
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
AC52-07NA27344
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015 Jan 21
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Riot, Vincent J. System and method for measuring fluorescence of a sample. United States: N. p., 2017. Web.
Riot, Vincent J. System and method for measuring fluorescence of a sample. United States.
Riot, Vincent J. Tue . "System and method for measuring fluorescence of a sample". United States. https://www.osti.gov/servlets/purl/1366507.
@article{osti_1366507,
title = {System and method for measuring fluorescence of a sample},
author = {Riot, Vincent J.},
abstractNote = {The present disclosure provides a system and a method for measuring fluorescence of a sample. The sample may be a polymerase-chain-reaction (PCR) array, a loop-mediated-isothermal amplification array, etc. LEDs are used to excite the sample, and a photodiode is used to collect the sample's fluorescence. An electronic offset signal is used to reduce the effects of background fluorescence and the noises from the measurement system. An integrator integrates the difference between the output of the photodiode and the electronic offset signal over a given period of time. The resulting integral is then converted into digital domain for further processing and storage.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {6}
}

Works referenced in this record:

Syringe Pump
patent, June 1969


Manifold with check valve positioned within manifold body
patent, August 2000


Digital offset corrector
patent, August 2001


Fluidics platform and method for sample preparation and analysis
patent, August 2014


Fluidics platform and method for sample preparation
patent, June 2016


Fluid dispensing system and valve control
patent-application, May 2002


Automatic threshold setting and baseline determination for real-time PCR
patent-application, August 2003


Digital signal processor-based detection system, method, and apparatus for optical tomography
patent-application, November 2005


Thermal Cycling System
patent-application, January 2008


Reagent Cartridge
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Method for the analysis of exclusive gene expression profile using a trace amount of sample
patent-application, October 2009


Apparatus And Method For Reverse Power Regulation With Measured Source Side Voltage
patent-application, February 2010


Method of Concentrating Beads in a Droplet
patent-application, May 2011