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Title: Enhanced processive cellulases

Abstract

Nucleic acid sequences encoding chimeric polypeptides that exhibit enhanced cellulase activities are disclosed herein. These nucleic acids may be expressed in hosts such as fungi, which in turn may be cultured to produce chimeric polypeptides. Also disclosed are chimeric polypeptides and their use in the degradation of cellulosic materials.

Inventors:
; ; ; ; ; ; ; ; ; ;
Issue Date:
Research Org.:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1364629
Patent Number(s):
9683248
Application Number:
14/365,200
Assignee:
Alliance for Sustainable Energy, LLC
Patent Classifications (CPCs):
C - CHEMISTRY C10 - PETROLEUM, GAS OR COKE INDUSTRIES C10L - FUELS NOT OTHERWISE PROVIDED FOR
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC36-08G028308
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Dec 17
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Adney, William S., Beckham, Gregg T., Jarvis, Eric, Himmel, Michael E., Decker, Stephen R., Linger, Jeffrey G., Podkaminer, Kara, Baker, John O., Taylor, II, Larry, Xu, Qi, and Singh, Arjun. Enhanced processive cellulases. United States: N. p., 2017. Web.
Adney, William S., Beckham, Gregg T., Jarvis, Eric, Himmel, Michael E., Decker, Stephen R., Linger, Jeffrey G., Podkaminer, Kara, Baker, John O., Taylor, II, Larry, Xu, Qi, & Singh, Arjun. Enhanced processive cellulases. United States.
Adney, William S., Beckham, Gregg T., Jarvis, Eric, Himmel, Michael E., Decker, Stephen R., Linger, Jeffrey G., Podkaminer, Kara, Baker, John O., Taylor, II, Larry, Xu, Qi, and Singh, Arjun. Tue . "Enhanced processive cellulases". United States. https://www.osti.gov/servlets/purl/1364629.
@article{osti_1364629,
title = {Enhanced processive cellulases},
author = {Adney, William S. and Beckham, Gregg T. and Jarvis, Eric and Himmel, Michael E. and Decker, Stephen R. and Linger, Jeffrey G. and Podkaminer, Kara and Baker, John O. and Taylor, II, Larry and Xu, Qi and Singh, Arjun},
abstractNote = {Nucleic acid sequences encoding chimeric polypeptides that exhibit enhanced cellulase activities are disclosed herein. These nucleic acids may be expressed in hosts such as fungi, which in turn may be cultured to produce chimeric polypeptides. Also disclosed are chimeric polypeptides and their use in the degradation of cellulosic materials.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {6}
}

Works referenced in this record:

Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum
patent, November 2008


Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
patent, October 2011


Polypeptides having endoglucanase activity and polynucleotides encoding same
patent, November 2011


A family of thermostable fungal cellulases created by structure-guided recombination
journal, March 2009


SCHEMA Recombination of a Fungal Cellulase Uncovers a Single Mutation That Contributes Markedly to Stability
journal, July 2009


Binding modules alter the activity of chimeric cellulases: Effects of biomass pretreatment and enzyme source
journal, July 2010


A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei
journal, January 1987


Trichoderma reesei cellobiohydrolase I with an endoglucanase cellulose-binding domain: action on bacterial microcrystalline cellulose
journal, September 1997


Cellulases and biofuels
journal, June 2009